Effects of exercise preconditioning on NLRP3 and mitochondrial fission in isoproterenol-induced myocardial infarcted rats

AbstractMyocardial infarction is a common disease that causes morbidity and mortality in human. Exercise training is an effective strategy to improve cardioprotection. The signaling pathways of exercise preconditioning on the reduction of MI-induced cardiac injuries is one of the topics that has attracted a lot of attention. The purpose of this study was to investigate the effects of exercise preconditioning on cardiac damage and dynamin-related protein 1 (Drp1) as an effective factor for activating the NOD-, LRR-, and pyrin domain-containing protein 3 (NLRP3) following myocardial infarction (MI). Twenty male Wistar rats were randomly divided into 4 groups of high-intensity interval training  + myocardial infarction (HIIT + MI), control + myocardial infarction (CON + MI), HIIT, and sham. Training groups performed 4 weeks (5 days per week) of HIIT. The training protocol consisted of 10*1-min running intervals were separated by a 2-min rest. The training intensity varied every week. For induction of MI, an injection of isoproterenol was used. Creatine kinase-myoglobin binding (CK-MB), lactate dehydrogenase (LDH), Drp1, and NLRP3 gene expression were measured. The results of the present study showed that CK-MB and LDH in CON + MI group were significantly higher than in the HIIT + MI group (P˂ 0.05). Myocardial infarction results in a significant increase in Drp1 gene expression in the CON + MI and HIIT + MI groups relative to the sham group. Th...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research