Mouse cardiomyocyte isolation: Filling the age gaps

In 1960, California, Isaac Harary and Barbara Farley carefully reported the shapes and beating of cells freshly extracted from young rat hearts [1]. This may, they wrote, provide a unique system to study the mammalian heart. Today, isolated cardiomyocytes (CM) are a central component for the molecular investigation of cardiac biology and disease. To this aim, the reliable isolation of high yields of quality cells is challenging, and a constant limitation. CMs in the myocardium associate intimately with neighbouring cells and matrix (ECM) and are sensitive to metabolic, mechanical, ionic and enzymatic disturbances.

Source: Journal of Molecular and Cellular Cardiology - July 8, 2022 Category: Cytology Authors: Matthew Ackers-Johnson, Roger S. Foo, Davor Pavlovic Source Type: research