Human Endometrium ‐Derived Adventitial Cell Spheroid‐Loaded Antimicrobial Microneedles for Uterine Regeneration

The flexible microneedles fabricated with gelatin methacryloyl and lactoferrin exhibit rapid degradation and antimicrobial activity. Benefiting from microwells on microneedles, human endometrium-derived adventitial cells (En-ADVs) rapidly form 3D cell spheroids, displaying higher potentials of cell proliferation, differentiation, and migration in comparison to dissociated cells. Transplantation of En-ADV spheroid-loaded hierarchical microneedles (MN/En-ADV) patches efficiently repairs the injured uterus and restores the reproductive function in Asherman's syndrome model rats. AbstractAsherman's syndrome (AS) occurs as a consequence of severe damage to the endometrial basalis, usually leading to menstrual abnormalities, infertility, and recurrent miscarriage in women. Currently, human endometrium-derived adventitial cells (En-ADVs) are considered ideal seed cells with high pluripotency for regenerative medicine. However, critical issues such as noninvasive repair of tissues, targeting of native stem cells, and continuous action in the injured sites are not well resolved. Herein, En-ADV spheroid-loaded hierarchical microneedles (MN/En-ADV) for in situ intrauterine repair are developed. The flexible microneedles are fabricated with gelatin methacryloyl and lactoferrin, imparting the characteristics of rapid degradation and antimicrobial activity. Benefiting from an array of microwells on microneedles, En-ADVs can rapidly form 3D cell spheroids, which display higher potential for...
Source: Small - Category: Nanotechnology Authors: Tags: Research Article Source Type: research