Molecules, Vol. 27, Pages 4350: Melatonin Reverses the Warburg-Type Metabolism and Reduces Mitochondrial Membrane Potential of Ovarian Cancer Cells Independent of MT1 Receptor Activation

Molecules, Vol. 27, Pages 4350: Melatonin Reverses the Warburg-Type Metabolism and Reduces Mitochondrial Membrane Potential of Ovarian Cancer Cells Independent of MT1 Receptor Activation Molecules doi: 10.3390/molecules27144350 Authors: Maira Smaniotto Cucielo Roberta Carvalho Cesário Henrique Spaulonci Silveira Letícia Barbosa Gaiotte Sérgio Alexandre Alcantara dos Santos Debora Aparecida Pires de Campos Zuccari Fábio Rodrigues Ferreira Seiva Russel J. Reiter Luiz Gustavo de Almeida Chuffa Ovarian cancer (OC) is the most lethal gynecologic malignancy, and melatonin has shown various antitumor properties. Herein, we investigated the influence of melatonin therapy on energy metabolism and mitochondrial integrity in SKOV-3 cells and tested whether its effects depended on MT1 receptor activation. SKOV-3 cells were exposed to different melatonin concentrations, and experimental groups were divided as to the presence of MT1 receptors (melatonin groups) or receptor absence by RNAi silencing (siRNA MT1+melatonin). Intracellular melatonin levels increased after treatment with melatonin independent of the MT1. The mitochondrial membrane potential of SKOV-3 cells decreased in the group treated with the highest melatonin concentration. Melatonin reduced cellular glucose consumption, while MT1 knockdown increased its consumption. Interconversion of lactate to pyruvate increased after treatment with melatonin and was remarkable in siRNA MT1 groups. Moreover, la...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research