Molecules, Vol. 27, Pages 4350: Melatonin Reverses the Warburg-Type Metabolism and Reduces Mitochondrial Membrane Potential of Ovarian Cancer Cells Independent of MT1 Receptor Activation
Molecules, Vol. 27, Pages 4350: Melatonin Reverses the Warburg-Type Metabolism and Reduces Mitochondrial Membrane Potential of Ovarian Cancer Cells Independent of MT1 Receptor Activation
Molecules doi: 10.3390/molecules27144350
Authors:
Maira Smaniotto Cucielo
Roberta Carvalho Cesário
Henrique Spaulonci Silveira
Letícia Barbosa Gaiotte
Sérgio Alexandre Alcantara dos Santos
Debora Aparecida Pires de Campos Zuccari
Fábio Rodrigues Ferreira Seiva
Russel J. Reiter
Luiz Gustavo de Almeida Chuffa
Ovarian cancer (OC) is the most lethal gynecologic malignancy, and melatonin has shown various antitumor properties. Herein, we investigated the influence of melatonin therapy on energy metabolism and mitochondrial integrity in SKOV-3 cells and tested whether its effects depended on MT1 receptor activation. SKOV-3 cells were exposed to different melatonin concentrations, and experimental groups were divided as to the presence of MT1 receptors (melatonin groups) or receptor absence by RNAi silencing (siRNA MT1+melatonin). Intracellular melatonin levels increased after treatment with melatonin independent of the MT1. The mitochondrial membrane potential of SKOV-3 cells decreased in the group treated with the highest melatonin concentration. Melatonin reduced cellular glucose consumption, while MT1 knockdown increased its consumption. Interconversion of lactate to pyruvate increased after treatment with melatonin and was remarkable in siRNA MT1 groups. Moreover, la...
Source: Molecules - Category: Chemistry Authors: Maira Smaniotto Cucielo Roberta Carvalho Ces ário Henrique Spaulonci Silveira Let ícia Barbosa Gaiotte S érgio Alexandre Alcantara dos Santos Debora Aparecida Pires de Campos Zuccari F ábio Rodrigues Ferreira Seiva Russel J. Reiter Luiz Gustavo de Alm Tags: Article Source Type: research