A comprehensive analysis of the microbiota composition and host driver gene mutations in colorectal cancer

AbstractStudies of both, microbiota and target therapy associated with gene mutations in colorectal cancer, (CRC) have attracted increasing attention. However, only a few of them analyzed the combined effects on CRC. we analyzed differences in intestinal microbiota of 44 colorectal cancer patients and 20 healthy controls (HC) using 16S rRNA gene sequencing of fecal samples. For 39 of the CRC patients, targeted Next Generation Sequencing (NGS) was carried out at formalin fixed paraffin embedded (FFPE) samples to identify somatic mutation profiles. Compared to the HC group, the microbial diversity of CRC patients was significantly lower. In the CRC group, we found a microbiome that was significantly enriched for strains ofBifidobacterium,Bacteroides, andMegasphaera whereas in the HC group the abundance ofCollinsella,Faecalibacterium, andAgathobacter strains was higher. Among the mutations detected in the CRC group, the APC gene had the highest mutation rate (77%, 30/39). We found that theKRAS mutant type was closely associated withFaecalibacterium,Roseburia,Megamonas,Lachnoclostridium, andHarryflintia. Notably, Spearman correlation analysis showed thatKRAS mutations were negatively correlated with the existence ofBifidobacterium and positively correlated withFaecalibacterium. By employing 16S rRNA gene sequencing, we identified more unique features of microbiota profiles in CRC patients. For the first time, our study showed that gene mutations could directly be linked to the mi...
Source: Investigational New Drugs - Category: Drugs & Pharmacology Source Type: research