Engineering Pancreatic Islets to Transiently Codisplay on Their Surface Thrombomodulin and CD47 Immunomodulatory Proteins as a Means of Mitigating Instant Blood-Mediated Inflammatory Reaction following Intraportal Transplantation
This study aimed to test whether islets can be coengineered with SA-TM and SA-CD47 molecules as a combinatorial approach to improve engraftment by inhibiting IBMIR. Mouse islets were effectively coengineered with both molecules without a detectable negative impact on their viability and metabolic function. Coengineered islets were refractory to destruction by IBMIR ex vivo and showed enhanced engraftment and sustained function in a marginal mass syngeneic intraportal transplantation model. Improved engraftment correlated with a reduction in intragraft innate immune infiltrates, particularly neutrophils and M1 macrophages. ...
Source: Journal of Immunology - May 6, 2024 Category: Allergy & Immunology Authors: Ali Turan Mohammad Tarique Lei Zhang Shadab Kazmi Vahap Ulker Mebrahtu G Tedla Darshan Badal Esma S Yolcu Haval Shirwan Source Type: research
IRF7 Exacerbates Candida albicans Infection by Compromising CD209-Mediated Phagocytosis and Autophagy-Mediated Killing in Macrophages
J Immunol. 2024 May 6:ji2300826. doi: 10.4049/jimmunol.2300826. Online ahead of print.ABSTRACTIFN regulatory factor 7 (IRF7) exerts anti-infective effects by promoting the production of IFNs in various bacterial and viral infections, but its role in highly morbid and fatal Candida albicans infections is unknown. We unexpectedly found that Irf7 gene expression levels were significantly upregulated in tissues or cells after C. albicans infection in humans and mice and that IRF7 actually exacerbates C. albicans infection in mice independent of its classical function in inducing IFNs production. Compared to controls, Irf7-/- m...
Source: Journal of Immunology - May 6, 2024 Category: Allergy & Immunology Authors: Furong Qing Lina Sui Wenji He Yayun Chen Li Xu Liangmei He Qiuxiang Xiao Tianfu Guo Zhiping Liu Source Type: research
Hyperosmotic Stress Induces Inflammation and Excessive Th17 Response to Blunt T-Cell Immunity in Tilapia
J Immunol. 2024 May 3:ji2300251. doi: 10.4049/jimmunol.2300251. Online ahead of print.ABSTRACTDespite the advances in study on osmotic physiology in bony fish, the mechanism by which the immune system, especially T-cell immunity, adapts and responds to osmotic stress remains unknown. In the current study, we investigated the response of T cells to hyperosmotic stress in the bony fish Nile tilapia (Oreochromis niloticus). As a euryhaline fish, tilapia was able to adapt to a wide range of salinities; however, hypertonic stress caused inflammation and excessive T-cell activation. Furthermore, hypertonic stress increased the e...
Source: Journal of Immunology - May 3, 2024 Category: Allergy & Immunology Authors: Jiansong Zhang Xiaodan Wang Kang Li Wenzhuo Rao Xinying Jiao Wei Liang Haiyou Gao Ding Wang Yi Cao Xiumei Wei Jialong Yang Source Type: research
Nucleic Acid Sensing by STING Induces an IFN-like Antiviral Response in a Marine Invertebrate
In this study, we present evidence demonstrating that STING, an ortholog found in a marine invertebrate (shrimp) called Litopenaeus vannamei, can directly detect DNA and initiate an IFN-like antiviral response. Unlike its homologs in other eukaryotic organisms, which exclusively function as sensors for cyclic dinucleotides, shrimp STING has the ability to bind to both double-stranded DNA and cyclic dinucleotides, including 2'3'-cGAMP. In vivo, shrimp STING can directly sense DNA nucleic acids from an infected virus, accelerate IFN regulatory factor dimerization and nuclear translocation, induce the expression of an IFN fun...
Source: Journal of Immunology - May 3, 2024 Category: Allergy & Immunology Authors: Haoyang Li Xuanzheng Di Sheng Wang Qinyao Li Shaoping Weng Jianguo He Chaozheng Li Source Type: research
The TNFSF12/TWEAK Modulates Colonic Inflammatory Fibroblast Differentiation and Promotes Fibroblast-Monocyte Interactions
In this study, we explore its role as a driver of inflammatory responses in fibroblasts and its contribution to fibroblast-monocyte interaction using human primary colonic fibroblasts, THP-1 and primary monocytes. Recombinant human TWEAK induced the expression of cytokines, chemokines, and immune receptors in primary colonic fibroblasts. The TWEAK upregulated transcriptome shared 29% homology with a previously published transcriptional profile of inflammatory fibroblasts from ulcerative colitis. TWEAK elevated surface expression of activated fibroblast markers and adhesion molecules (podoplanin [PDPN], ICAM-1, and VCAM-1) ...
Source: Journal of Immunology - May 3, 2024 Category: Allergy & Immunology Authors: Carlos Matellan Ciar án Kennedy Miren Itxaso Santiago-Vela Johanna Hochegger M éabh B Ní Chathail Amanda Wu Christopher Shannon Helen M Roche Seema S Aceves Catherine Godson Mario C Manresa Source Type: research
Hyperosmotic Stress Induces Inflammation and Excessive Th17 Response to Blunt T-Cell Immunity in Tilapia
J Immunol. 2024 May 3:ji2300251. doi: 10.4049/jimmunol.2300251. Online ahead of print.ABSTRACTDespite the advances in study on osmotic physiology in bony fish, the mechanism by which the immune system, especially T-cell immunity, adapts and responds to osmotic stress remains unknown. In the current study, we investigated the response of T cells to hyperosmotic stress in the bony fish Nile tilapia (Oreochromis niloticus). As a euryhaline fish, tilapia was able to adapt to a wide range of salinities; however, hypertonic stress caused inflammation and excessive T-cell activation. Furthermore, hypertonic stress increased the e...
Source: Journal of Immunology - May 3, 2024 Category: Allergy & Immunology Authors: Jiansong Zhang Xiaodan Wang Kang Li Wenzhuo Rao Xinying Jiao Wei Liang Haiyou Gao Ding Wang Yi Cao Xiumei Wei Jialong Yang Source Type: research
Nucleic Acid Sensing by STING Induces an IFN-like Antiviral Response in a Marine Invertebrate
In this study, we present evidence demonstrating that STING, an ortholog found in a marine invertebrate (shrimp) called Litopenaeus vannamei, can directly detect DNA and initiate an IFN-like antiviral response. Unlike its homologs in other eukaryotic organisms, which exclusively function as sensors for cyclic dinucleotides, shrimp STING has the ability to bind to both double-stranded DNA and cyclic dinucleotides, including 2'3'-cGAMP. In vivo, shrimp STING can directly sense DNA nucleic acids from an infected virus, accelerate IFN regulatory factor dimerization and nuclear translocation, induce the expression of an IFN fun...
Source: Journal of Immunology - May 3, 2024 Category: Allergy & Immunology Authors: Haoyang Li Xuanzheng Di Sheng Wang Qinyao Li Shaoping Weng Jianguo He Chaozheng Li Source Type: research
The TNFSF12/TWEAK Modulates Colonic Inflammatory Fibroblast Differentiation and Promotes Fibroblast-Monocyte Interactions
In this study, we explore its role as a driver of inflammatory responses in fibroblasts and its contribution to fibroblast-monocyte interaction using human primary colonic fibroblasts, THP-1 and primary monocytes. Recombinant human TWEAK induced the expression of cytokines, chemokines, and immune receptors in primary colonic fibroblasts. The TWEAK upregulated transcriptome shared 29% homology with a previously published transcriptional profile of inflammatory fibroblasts from ulcerative colitis. TWEAK elevated surface expression of activated fibroblast markers and adhesion molecules (podoplanin [PDPN], ICAM-1, and VCAM-1) ...
Source: Journal of Immunology - May 3, 2024 Category: Allergy & Immunology Authors: Carlos Matellan Ciar án Kennedy Miren Itxaso Santiago-Vela Johanna Hochegger M éabh B Ní Chathail Amanda Wu Christopher Shannon Helen M Roche Seema S Aceves Catherine Godson Mario C Manresa Source Type: research
Hyperosmotic Stress Induces Inflammation and Excessive Th17 Response to Blunt T-Cell Immunity in Tilapia
J Immunol. 2024 May 3:ji2300251. doi: 10.4049/jimmunol.2300251. Online ahead of print.ABSTRACTDespite the advances in study on osmotic physiology in bony fish, the mechanism by which the immune system, especially T-cell immunity, adapts and responds to osmotic stress remains unknown. In the current study, we investigated the response of T cells to hyperosmotic stress in the bony fish Nile tilapia (Oreochromis niloticus). As a euryhaline fish, tilapia was able to adapt to a wide range of salinities; however, hypertonic stress caused inflammation and excessive T-cell activation. Furthermore, hypertonic stress increased the e...
Source: Journal of Immunology - May 3, 2024 Category: Allergy & Immunology Authors: Jiansong Zhang Xiaodan Wang Kang Li Wenzhuo Rao Xinying Jiao Wei Liang Haiyou Gao Ding Wang Yi Cao Xiumei Wei Jialong Yang Source Type: research
Nucleic Acid Sensing by STING Induces an IFN-like Antiviral Response in a Marine Invertebrate
In this study, we present evidence demonstrating that STING, an ortholog found in a marine invertebrate (shrimp) called Litopenaeus vannamei, can directly detect DNA and initiate an IFN-like antiviral response. Unlike its homologs in other eukaryotic organisms, which exclusively function as sensors for cyclic dinucleotides, shrimp STING has the ability to bind to both double-stranded DNA and cyclic dinucleotides, including 2'3'-cGAMP. In vivo, shrimp STING can directly sense DNA nucleic acids from an infected virus, accelerate IFN regulatory factor dimerization and nuclear translocation, induce the expression of an IFN fun...
Source: Journal of Immunology - May 3, 2024 Category: Allergy & Immunology Authors: Haoyang Li Xuanzheng Di Sheng Wang Qinyao Li Shaoping Weng Jianguo He Chaozheng Li Source Type: research
The TNFSF12/TWEAK Modulates Colonic Inflammatory Fibroblast Differentiation and Promotes Fibroblast-Monocyte Interactions
In this study, we explore its role as a driver of inflammatory responses in fibroblasts and its contribution to fibroblast-monocyte interaction using human primary colonic fibroblasts, THP-1 and primary monocytes. Recombinant human TWEAK induced the expression of cytokines, chemokines, and immune receptors in primary colonic fibroblasts. The TWEAK upregulated transcriptome shared 29% homology with a previously published transcriptional profile of inflammatory fibroblasts from ulcerative colitis. TWEAK elevated surface expression of activated fibroblast markers and adhesion molecules (podoplanin [PDPN], ICAM-1, and VCAM-1) ...
Source: Journal of Immunology - May 3, 2024 Category: Allergy & Immunology Authors: Carlos Matellan Ciar án Kennedy Miren Itxaso Santiago-Vela Johanna Hochegger M éabh B Ní Chathail Amanda Wu Christopher Shannon Helen M Roche Seema S Aceves Catherine Godson Mario C Manresa Source Type: research
BHLHE40 Regulates Myeloid Cell Polarization through IL-10-Dependent and -Independent Mechanisms
In this study, we report that BHLHE40 is required in myeloid cells exposed to GM-CSF, an abundant cytokine in the lung, to promote the expression of genes associated with a proinflammatory state and better control of M. tuberculosis infection. Loss of Bhlhe40 expression in murine bone marrow-derived myeloid cells cultured in the presence of GM-CSF results in lower levels of proinflammatory associated signaling molecules IL-1β, IL-6, IL-12, TNF-α, inducible NO synthase, IL-2, KC, and RANTES, as well as higher levels of the anti-inflammatory-associated molecules MCP-1 and IL-10 following exposure to heat-killed M. tubercul...
Source: Journal of Immunology - April 29, 2024 Category: Allergy & Immunology Authors: Skyler V Hendrix Yassin Mreyoud Michael E McNehlan Asya Smirnov Sthefany M Chavez Brian Hie Megan M Chamberland Tara R Bradstreet Ashlee M Webber Darren Kreamalmeyer Reshma Taneja Bryan D Bryson Brian T Edelson Christina L Stallings Source Type: research
A Significant Contribution of the Classical Pathway of Complement in SARS-CoV-2 Neutralization of Convalescent and Vaccinee Sera
In this study, we report that the SARS-CoV-2 neutralization titers of human convalescent sera were relatively consistent across all disease states except for severe COVID-19, which yielded significantly higher neutralization titers. Furthermore, we show that heat inactivation of human serum significantly lowered neutralization activity in a live virus SARS-CoV-2 neutralization assay. Heat inactivation of human convalescent serum was shown to inactivate complement proteins, and the contribution of complement in SARS-CoV-2 neutralization was often >50% of the neutralizing activity of human sera without heat inactivation a...
Source: Journal of Immunology - April 29, 2024 Category: Allergy & Immunology Authors: Patrick Budylowski Serena L L Chau Arinjay Banerjee Furkan Guvenc Reuben Samson Queenie Hu Lindsey Fiddes Laurie Seifried Gary Chao Megan Buchholz Antonio Estacio Patti Lou Cheatley Katerina Pavenski Christopher J Patriquin Yanling Liu Salma Sheikh-Mohame Source Type: research
Polyclonally Derived Alloantigen-Specific T Regulatory Cells Exhibit Target-Specific Suppression and Capture MHC Class II from Dendritic Cells
J Immunol. 2024 Apr 29:ji2300780. doi: 10.4049/jimmunol.2300780. Online ahead of print.ABSTRACTFoxp3+ T regulatory (Treg) cells prevent allograft rejection and graft-versus-host disease. Although polyclonal Tregs have been used both in animal models and in humans, the fine specificity of their suppressive function is poorly defined. We have generated mouse recipient-derived alloantigen-specific Tregs in vitro and explored the fine specificity of their suppressive function and their mechanism of action in vitro and in vivo. In vitro, when alloantigen and peptide Ag were both presented on the same dendritic cell, both respon...
Source: Journal of Immunology - April 29, 2024 Category: Allergy & Immunology Authors: Yoshihiro Oya Yasuyo Tanaka Takuya Nakazawa Ryutaro Matsumura Deborah D Glass Hiroshi Nakajima Ethan M Shevach Source Type: research
BHLHE40 Regulates Myeloid Cell Polarization through IL-10-Dependent and -Independent Mechanisms
In this study, we report that BHLHE40 is required in myeloid cells exposed to GM-CSF, an abundant cytokine in the lung, to promote the expression of genes associated with a proinflammatory state and better control of M. tuberculosis infection. Loss of Bhlhe40 expression in murine bone marrow-derived myeloid cells cultured in the presence of GM-CSF results in lower levels of proinflammatory associated signaling molecules IL-1β, IL-6, IL-12, TNF-α, inducible NO synthase, IL-2, KC, and RANTES, as well as higher levels of the anti-inflammatory-associated molecules MCP-1 and IL-10 following exposure to heat-killed M. tubercul...
Source: Journal of Immunology - April 29, 2024 Category: Allergy & Immunology Authors: Skyler V Hendrix Yassin Mreyoud Michael E McNehlan Asya Smirnov Sthefany M Chavez Brian Hie Megan M Chamberland Tara R Bradstreet Ashlee M Webber Darren Kreamalmeyer Reshma Taneja Bryan D Bryson Brian T Edelson Christina L Stallings Source Type: research
