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Infectious Disease: Gastroenteritis
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Total 36194 results found since Jan 2013.
Investigation of the potential roles of adipose stem cells and substances of natural origin in the healing process of E. coli infected wound model in Rats
Tissue Cell. 2023 Sep 4;85:102214. doi: 10.1016/j.tice.2023.102214. Online ahead of print.ABSTRACTSkin infections by pathogenic microorganisms are a serious problem due to the potential of dissemination through the bloodstream to various organs causing toxic effects that may be up to mortality. Escherichia coli (E. coli) is one of the most predominant Gram-negative bacterial species present globally with great attention for investigation. The current study is designed to investigate the possible role of adipose tissue-derived stem cells (ADSCs), as well as natural products such as Trichoderma viride (T. viride) extract, Sa...
Source: Tissue and Cell - September 10, 2023 Category: Cytology Authors: Heba R Hashem Basma H Amin Mohammed Yosri Source Type: research
Structure-guided approach to modify the substrate specificity of the protein human deglycase-1 (hDJ-1)
Biochem Biophys Res Commun. 2023 Sep 1;679:122-128. doi: 10.1016/j.bbrc.2023.08.058. Online ahead of print.ABSTRACTGlycation is a non-enzymatic reaction wherein sugars or dicarbonyls such as methylglyoxal (MGO) and glyoxal (GO) react with proteins, leading to protein inactivation. The hydrolysing enzyme human deglycase-1 (hDJ-1) is reported to decrease glycative stress by deglycating the modified proteins, specifically at cysteine, lysine, and arginine sites. This specificity of hDJ-1 is thought to be regulated by its active site cysteine residue (Cys106). Structural analysis of hDJ-1 by molecular docking and simulation st...
Source: Biochemical and Biophysical Research communications - September 9, 2023 Category: Biochemistry Authors: Sera A Fernandes Subrata Dasgupta Rashmi S Tupe Ejaj K Pathan Source Type: research
Encapsulation of Dicranopteris linearis extract using cellulose microparticles for antiulcer medication
Int J Biol Macromol. 2023 Sep 7:126795. doi: 10.1016/j.ijbiomac.2023.126795. Online ahead of print.ABSTRACTDicranopteris linearis (DL) is a fern in the Gleicheniaceae family, locally known as resam by the Malay community. It has numerous pharmacological benefits, with antiulcer and gastroprotective properties. Peptic ulcer is a chronic and recurring disease that significantly impacts morbidity and mortality, affecting nearly 20 % of the world's population. Despite the effectiveness of peptic ulcer drugs, there is no perfect treatment for the ailment. Encapsulation is an advanced technique that can treat peptic ulcers by in...
Source: International Journal of Biological Macromolecules - September 9, 2023 Category: Biochemistry Authors: Anis Adilah Ahmad Khairul Farihan Kasim Subash C B Gopinath Periasamy Anbu Noor Soffalina Sofian Seng Source Type: research
Biomimetic helical fiber cellulose acetate/thermoplastic polyurethanes photodynamic antibacterial membrane: Synthesis, characterization, and antibacterial application
This study designed a novel co-electrospun cellulose acetate (CA)/thermoplastic polyurethane (TPU) photodynamic helical fiber antibacterial membrane as a potential environmentally friendly medical protective material. A central combined design method (CCD) based on response surface methodology (RSM) was used to analyze essential variables' influence. The optimized parameters for CCD were TPU (wt%) 11.68 %, CA (wt%) 13.89 %, DMAc/ACE volume ratio 0.147, LiCl (wt%) 1.39 %, and voltage (kV) 14.43 V. Pitch and pitch diameter were the response process as the critical output variable. The membranes were characterized by SEM, TG,...
Source: International Journal of Biological Macromolecules - September 9, 2023 Category: Biochemistry Authors: Xianjin Hu Lu Yang Ying Zhang Bingbing Shou Hai-Tao Ren Jia-Horng Lin Ching-Wen Lou Ting-Ting Li Source Type: research
Polymeric curcumin nanospheres for lysozyme aggregation inhibition, antibacterial, and wound healing applications
Environ Sci Pollut Res Int. 2023 Sep 9. doi: 10.1007/s11356-023-29160-x. Online ahead of print.ABSTRACTThe present study reports highly stable polymeric nanoparticles comprising curcumin and polyvinylpyrrolidone, and then conjugated with gold nanoparticles, resulting in C-PVP and C-PVP-Au, respectively. The synthesized conjugates C-PVP and C-PVP-Au were investigated for amyloid aggregation inhibition activity, antimicrobial activity, and wound healing applications. The anti-amyloidogenic capacity of nanoconjugates were studied for model protein, hen egg-white lysozyme (HEWL). The ThT binding assay, fibril size measurement,...
Source: Environmental Science and Pollution Research International - September 9, 2023 Category: Environmental Health Authors: Pranita Rananaware Samir Bauri Rangappa Keri Monalisa Mishra Varsha Brahmkhatri Source Type: research
Escherichia coli BL21(DE3) optimized deletion mutant as the host for whole-cell biotransformation of N ‑acetyl‑D‑neuraminic acid
In this study, via single-plasmid biotransformation system, we showed that the AGE gene BT0453, cloned from human gut microorganism Bacteroides thetaiotaomicron VPI-5482, showed the highest biotransformation yield among the AGE genes tested; and there is no clear Neu5Ac yield difference between the BT0453 coupled with one aldolase coding nanA gene and two nanA genes. Next, Escherichia coli chromosomal genes involved in substrate degradation, product exportation and pH change were deleted via recombineering and CRISPR/Cas9. With the final E. coli BL21(DE3) ΔnanA Δnag ΔpoxB as host, a significant 16.5% yield improvement w...
Source: Biotechnology Letters - September 9, 2023 Category: Biotechnology Authors: Qiong Zhang Jiao Zhang Yanhong Shao Guangdong Shang Source Type: research
Vacuolar localisation of anthocyanin pigmentation in microgreen cotyledons of basil, cabbage and mustard greens does not impact on colonisation by Shiga-toxigenic Escherichia coli O157:H7
Food Microbiol. 2023 Dec;116:104367. doi: 10.1016/j.fm.2023.104367. Epub 2023 Aug 21.ABSTRACTMicrogreens, the immature plants harvested after a few weeks of growth, are perceived as a heathy, nutritious food ingredient but may be susceptible to colonisation by human pathogens including Shiga-toxigenic Escherichia coli (STEC). Some microgreen cultivars accumulate anthocyanins or secrete essential oils which, when extracted or purified, have been reported to inhibit bacterial growth. Therefore, the impact of anthocyanins on bacterial colonisation by STEC (Sakai) was compared for three species that have pigmented cultivars: b...
Source: Food Microbiology - September 9, 2023 Category: Food Science Authors: Kathryn M Wright Jacqueline Marshall Peter J Wright Nicola J Holden Source Type: research
Prevalence of ciprofloxacin resistance and associated genetic determinants differed among Campylobacter isolated from human and poultry meat sources in Pennsylvania
Food Microbiol. 2023 Dec;116:104349. doi: 10.1016/j.fm.2023.104349. Epub 2023 Aug 3.ABSTRACTPoultry is the primary source of Campylobacter infections and severe campylobacteriosis cases are treated with macrolides and fluoroquinolones. However, these drugs are less effective against antimicrobial-resistant strains. Here, we investigated the prevalence of phenotypic antimicrobial resistance and associated resistance genetic determinants in Campylobacter isolates collected from human clinical (N = 123) and meat (N = 80) sources in Pennsylvania in 2017 and 2018. Our goal was to assess potential differences in the prevalence o...
Source: Food Microbiology - September 9, 2023 Category: Food Science Authors: Runan Yan Nkuchia M M'ikanatha Irving Nachamkin Lauren K Hudson Thomas G Denes Jasna Kovac Source Type: research
Detection of resistance and virulence plasmids in Campylobacter coli and Campylobacter jejuni isolated from North Carolina food animal production, 2018-2019
Food Microbiol. 2023 Dec;116:104348. doi: 10.1016/j.fm.2023.104348. Epub 2023 Aug 11.ABSTRACTCampylobacter remains the leading cause of bacterial foodborne illness in the U.S. and worldwide. Campylobacter plasmids may play a significant role in antimicrobial resistance (AMR) and virulence factor distribution, and potentially drive rapid adaptation. C. coli (n = 345) and C. jejuni (n = 199) isolates collected from live cattle, swine, turkey, and chickens, poultry carcasses at production, and retail meat in N.C. were analyzed to determine plasmid prevalence, extrachromosomal virulence and AMR genes, and the phylogeny of asse...
Source: Food Microbiology - September 9, 2023 Category: Food Science Authors: Dawn M Hull Erin Harrel Lyndy Harden Siddhartha Thakur Source Type: research
The efficiency of UV light-emitting diodes (UV-LED) in decontaminating Campylobacter and Salmonella and natural microbiota in chicken breast, compared to a UV pilot-plant scale device
This study investigated the combined effect of Ultraviolet (UV) light-emitting diode (LED) technology treatment with refrigerated storage of chicken breast meat over 7 days on Campylobacter jejuni, Salmonella enterica serovar Typhimurium, total viable counts (TVC) and total Enterobacteriaceae counts (TEC). An optimised UV-LED treatment at 280 nm for 6 min decreased inoculated S. Typhimurium and C. jejuni populations by 0.6-0.64 log CFU/g, and TVC and TEC population by 1-1.2 log CFU/g in chicken samples. During a 7-day storage at 4 °C, a 0.73 log reduction in C. jejuni was achieved compared with non-treated samples. Moreov...
Source: Food Microbiology - September 9, 2023 Category: Food Science Authors: Arturo B Soro Daniel Ekhlas Sajad Shokri Ming Ming Yem Rui Chao Li Soukaina Barroug Shay Hannon Paul Whyte Declan J Bolton Catherine M Burgess Paula Bourke Brijesh K Tiwari Source Type: research
Modelling norovirus dynamics within oysters emphasises potential food safety issues associated with current testing & amp; depuration protocols
Food Microbiol. 2023 Dec;116:104363. doi: 10.1016/j.fm.2023.104363. Epub 2023 Aug 18.ABSTRACTNorovirus is a significant global cause of viral gastroenteritis, with raw oyster consumption often linked to such outbreaks due to their filter-feeding in harvest waters. National water quality and depuration/relaying times are often classified using Escherichia coli, a poor proxy for norovirus levels in shellfish. The current norovirus assay is limited to only the digestive tracts of oysters, meaning the total norovirus load of an oyster may differ from reported results. These limitations motivated this work, building upon previo...
Source: Food Microbiology - September 9, 2023 Category: Food Science Authors: Paul McMenemy Adam Kleczkowski Nick G H Taylor Source Type: research
Escherichia coli BL21(DE3) optimized deletion mutant as the host for whole-cell biotransformation of N ‑acetyl‑D‑neuraminic acid
In this study, via single-plasmid biotransformation system, we showed that the AGE gene BT0453, cloned from human gut microorganism Bacteroides thetaiotaomicron VPI-5482, showed the highest biotransformation yield among the AGE genes tested; and there is no clear Neu5Ac yield difference between the BT0453 coupled with one aldolase coding nanA gene and two nanA genes. Next, Escherichia coli chromosomal genes involved in substrate degradation, product exportation and pH change were deleted via recombineering and CRISPR/Cas9. With the final E. coli BL21(DE3) ΔnanA Δnag ΔpoxB as host, a significant 16.5% yield improvement w...
Source: Biotechnology Letters - September 9, 2023 Category: Biotechnology Authors: Qiong Zhang Jiao Zhang Yanhong Shao Guangdong Shang Source Type: research
Ability of Latilactobacillus curvatus FAM25164 to produce tryptamine: Identification of a novel tryptophan decarboxylase
This study describes a novel bacterial tryptophan decarboxylase (EC 4.1.1.105) that may be responsible for tryptamine formation in cheese.PMID:37689414 | DOI:10.1016/j.fm.2023.104343
Source: Food Microbiology - September 9, 2023 Category: Food Science Authors: Stefan Irmler Tharmatha Bavan Eliane Binz Reto Portmann Source Type: research
Encapsulation of Dicranopteris linearis extract using cellulose microparticles for antiulcer medication
Int J Biol Macromol. 2023 Sep 7:126795. doi: 10.1016/j.ijbiomac.2023.126795. Online ahead of print.ABSTRACTDicranopteris linearis (DL) is a fern in the Gleicheniaceae family, locally known as resam by the Malay community. It has numerous pharmacological benefits, with antiulcer and gastroprotective properties. Peptic ulcer is a chronic and recurring disease that significantly impacts morbidity and mortality, affecting nearly 20 % of the world's population. Despite the effectiveness of peptic ulcer drugs, there is no perfect treatment for the ailment. Encapsulation is an advanced technique that can treat peptic ulcers by in...
Source: International Journal of Biological Macromolecules - September 9, 2023 Category: Biochemistry Authors: Anis Adilah Ahmad Khairul Farihan Kasim Subash C B Gopinath Periasamy Anbu Noor Soffalina Sofian Seng Source Type: research
Biomimetic helical fiber cellulose acetate/thermoplastic polyurethanes photodynamic antibacterial membrane: Synthesis, characterization, and antibacterial application
This study designed a novel co-electrospun cellulose acetate (CA)/thermoplastic polyurethane (TPU) photodynamic helical fiber antibacterial membrane as a potential environmentally friendly medical protective material. A central combined design method (CCD) based on response surface methodology (RSM) was used to analyze essential variables' influence. The optimized parameters for CCD were TPU (wt%) 11.68 %, CA (wt%) 13.89 %, DMAc/ACE volume ratio 0.147, LiCl (wt%) 1.39 %, and voltage (kV) 14.43 V. Pitch and pitch diameter were the response process as the critical output variable. The membranes were characterized by SEM, TG,...
Source: International Journal of Biological Macromolecules - September 9, 2023 Category: Biochemistry Authors: Xianjin Hu Lu Yang Ying Zhang Bingbing Shou Hai-Tao Ren Jia-Horng Lin Ching-Wen Lou Ting-Ting Li Source Type: research
