Filtered By:
Specialty: Biology
Cancer: Breast Cancer
This page shows you your search results in order of date.
Order by Relevance | Date
Total 21 results found since Jan 2013.
Co-delivery of EGFR and BRD4 siRNA by cell-penetrating peptides-modified redox-responsive complex in triple negative breast cancer cells
Publication date: Available online 10 December 2020Source: Life SciencesAuthor(s): Chi Zhang, Wen Yuan, Ying Wu, Xu Wan, Yanling Gong
Source: Life Sciences - December 13, 2020 Category: Biology Source Type: research
Proteomics evaluation of MDA-MB-231 breast cancer cells in response to RNAi-induced silencing of hPTTG
This study aims to evaluate cell proliferation and the downstream expression pattern of hPTTG1 gene at the mRNA and protein levels after specific down-regulation of hPTTG1 by siRNA.Main methodsThe human breast cancer MDA-MB-231 cell line was transfected with siRNA against hPTTG1. The mRNA and protein expression levels were examined by Real-time PCR and Western blot, respectively. The cell proliferation was assayed by MTS. To investigate the pattern of protein expression, total cellular protein was analyzed by 2D gel electrophoresis and mass spectroscopy. Subsequently, the possible biological consequences were determined by...
Source: Life Sciences - September 13, 2019 Category: Biology Source Type: research
Synergistic effects of 7-O-geranylquercetin and siRNAs on the treatment of human breast cancer
Publication date: Available online 19 April 2019Source: Life SciencesAuthor(s): Jiaxin Zuo, Yameng Jiang, Enxia Zhang, Yuling Chen, Ze Liang, Jie Zhu, Yinan Zhao, Hong Xu, Guoliang Liu, Jiasi Liu, Wei Wang, Shubiao Zhang, Yuhong ZhenAbstractAimsTo investigate the antitumor effect of 7-O-geranylquercetin (GQ) combining with survivin siRNA (siSuvi) or IL-10 siRNA (siIL-10) to breast cancer.Main methodsXenograft tumor model was established by subcutaneously inoculating human breast cancer MCF-7 cells in BALB/c nude mice. Transfection efficiency of siRNA mediated by cationic liposome CDO14 in MCF-7 cells and tumor bearing mice...
Source: Life Sciences - April 19, 2019 Category: Biology Source Type: research
Endoplasmic reticulum stress triggered by Soyasapogenol B promotes apoptosis and autophagy in colorectal cancer
Publication date: Available online 14 December 2018Source: Life SciencesAuthor(s): Luping Wang, Lu Yun, Xiaojun Wang, Liying Sha, Luning Wang, Yingying Sui, Hui ZhangAbstractAimColorectal cancer (CRC) is a common human malignancy which accounts for 600,000 deaths annually at the global level. Soyasapogenol B (Soy B), an ingredient of soybean, has been found to exert anti-proliferative activities in vitro in human breast cancer cells. The current study aimed to evaluate the efficacy of Soy B in suppressing CRC.Methods and materialsThe effect of Soy B on cell viability was assessed using the Cell Counting Kit-8 (CCK-8) assay...
Source: Life Sciences - December 14, 2018 Category: Biology Source Type: research
Fibulin-1 interacts with Sex Hormone Binding Globulin and is linked to less aggressive estrogen-dependent breast cancers
In this study we investigated the expression of fibulins, their functional relationship with SHBG and involvement in behavior of estrogen-sensitive breast cancer.Main methodsGene expression of fibulins was performed by Real time-PCR on two estrogen-sensitive breast cancer cell lines, MCF-7 and T47D. Fibulin-1 protein expression and localization were determined by Western blot and immunofluorescence. SHBG interaction with-fibulin-1 was assessed by GST-pull down assay. MCF-7 cell growth and gene expression, after fibulin-1 silencing by siRNA, were evaluated. Finally, the expression of fibulin-1 was correlated to clinical and...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research
Nrf2 signaling and autophagy are complementary in protecting breast cancer cells during glucose deprivation.
In this study, we explored protective mechanisms in breast cancer cells in the setting of glucose deprivation. While breast cancer cells (MCF7 and T47D) survive in glucose-free medium for three days or more, autophagy is induced in this setting. Blocking autophagy pharmacologically with chloroquine or by knock-out of an essential autophagy gene, such as Beclin 1 or ATG7, markedly reduces the ability of cells to survive during glucose deprivation. Autophagy previously was shown to degrade p62, a protein that sequesters KEAP1, and KEAP1 in turn sequesters Nrf2, a master regulator of the antioxidant response. Hence, we invest...
Source: Free Radical Biology and Medicine - April 9, 2018 Category: Biology Authors: Walker A, Singh A, Tully E, Woo J, Le A, Nguyen T, Biswal S, Sharma D, Gabrielson E Tags: Free Radic Biol Med Source Type: research
Bowhead: Bayesian modelling of cell velocity during concerted cell migration
by Mathias Engel, James Longden, Jesper Ferkinghoff-Borg, Xavier Robin, Gaye Sa ğınç, Rune Linding
Cell migration is a central biological process that requires fine coordination of molecular events in time and space. A deregulation of the migratory phenotype is also associated with pathological conditions including cancer where cell motility has a causal role in tumor spreading and metastasis f ormation. Thus cell migration is of critical and strategic importance across the complex disease spectrum as well as for the basic understanding of cell phenotype. Experimental studies of the migration of cells in monolayers are...
Source: PLoS Computational Biology - January 8, 2018 Category: Biology Authors: Mathias Engel Source Type: research
Nonsteroidal estrogen receptor isoform ‐selective biphenyls
Estrogen receptor (ER) has been a therapeutic target to treat ER‐positive breast cancer, most notably by agents known as selective estrogen receptor modulators (SERMs). However, resistance and severe adverse effects of known drugs gave impetus to the search for newer agents with better therapeutic profile. ERα and ERβ are two isoforms sharing 56% identity and having different physiological functions and expressions in various tissues. Only two residues differ in the active sites of the two isoforms motivating us to design isoform‐selective ligands. Guided by computational docking and molecular dynamics simulations, w...
Source: Chemical Biology and Drug Design - November 10, 2017 Category: Biology Authors: Seema Bhatnagar, Anjali Soni, Swati Kaushik, Megha Rikhi, Thankayyan Retnabai Santhoshkumar, Bhyravabhotla Jayaram Tags: RESEARCH ARTICLE Source Type: research
Non ‐steroidal Estrogen Receptor Isoform Selective Biphenyls
This article is protected by copyright. All rights reserved.
Novel substituted biphenyl‐2,6‐diethanones are designed and synthesized targeting ERα isoform.
MD simulations along with MM‐GBSA accounted for the binding selectivity of 3b towards ERα over ERβ.
Co‐treatment and E‐screen studies indicated the ERα selective nature of 3b and anti‐estrogenicity.
ERα siRNA silencing experiments further confirmed the isoform selectivity.
Source: Chemical Biology and Drug Design - October 20, 2017 Category: Biology Authors: Seema Bhatnagar, Anjali Soni, Swati Kaushik, Megha Rikhi, T R Santhosh Kumar, B. Jayaram Tags: Research Article Source Type: research
Biological effects of amphiphilic copolymer nanoparticle-encapsulated multi-target chemotherapeutic drugs on MCF-7 human breast cancer cells
ConclusionThese findings provide important insight into the biological effects of nanoparticles and their encapsulated drugs while demonstrate that metabolomics is a powerful approach to evaluate the biological effects of nano-drugs.
Source: Metabolomics - March 2, 2017 Category: Biology Source Type: research
Novel polyacrylate-based cationic nanoparticles for survivin siRNA delivery combined with mitoxantrone for treatment of breast cancer
Publication date: Available online 3 October 2016 Source:Biologicals Author(s): Sanam Arami, Majid Mahdavi, Mohammad Reza Rashidi, Marziyeh Fathi, Mohammad-saeid Hejazi, Nasser Samadi As a gene delivery method in breast cancer therapy, knocking down the undesired genes in the cancerous cells would be promising. Inhibitors of Apoptosis Protein (IAP) family genes are some of the genes whose responsibility is inhibition of apoptosis in cells. Silencing these genes seems to be helpful directing the tumor cells to death. siRNA sequence designed against survivin anti-apoptotic gene can play this role if carried to the cytoplasm...
Source: Biologicals - October 3, 2016 Category: Biology Source Type: research
A novel bisindole-PBD conjugate inhibits angiogenesis by regulating STAT3 and VEGF in breast cancer cells
Publication date: Available online 12 March 2016 Source:Life Sciences Author(s): Pranjal Sarma, M. Janaki Ramaiah, Dhananjaya Pal, Utpal Bhadra, Manika Pal Bhadra Aims Breast cancer is highly resistant to chemotherapeutic approach and hence, alternative strategies have been developed to fight against this heterogeneous group of disease. In particular, many studies have demonstrated about various drugs for the treatment of breast cancer. In our study, we assessed the anti-angiogenenic activities of Bisindole-PBD (5b) in MCF-7 and MDA-MB-231 cell lines. Main methods In vitro Endothelial Cell (HUVEC) Tube Formation As...
Source: Life Sciences - March 12, 2016 Category: Biology Source Type: research
Over-expression of miR-451a can enhance the sensitivity of breast cancer cells to tamoxifen by regulating 14-3-3ζ, estrogen receptor α, and autophagy
Publication date: Available online 17 February 2016 Source:Life Sciences Author(s): Zhen-Ru Liu, Yi Song, Li-Hong Wan, Yuan-Yuan Zhang, Li-Mimg Zhou Aim To investigate the effects and mechanisms of miR-451a in the tamoxifen (TAM) resistance of breast cancer cells. Materials and methods TAM sensitive cells (MCF-7) and resistant cells (LCC2) were employed in the study. The lentivirus vectors of Lv-miR-451a, Lv-miR-451a sponge, and Lv-miR-451a NC were employed to increase or decrease the expression of miR-451a, respectively. SiRNA to 14-3-3ζ was used to inhibit expression of 14-3-3ζ. MTT assay was utilized to detect...
Source: Life Sciences - February 18, 2016 Category: Biology Source Type: research
