Fibulin-1 interacts with Sex Hormone Binding Globulin and is linked to less aggressive estrogen-dependent breast cancers

In this study we investigated the expression of fibulins, their functional relationship with SHBG and involvement in behavior of estrogen-sensitive breast cancer.Main methodsGene expression of fibulins was performed by Real time-PCR on two estrogen-sensitive breast cancer cell lines, MCF-7 and T47D. Fibulin-1 protein expression and localization were determined by Western blot and immunofluorescence. SHBG interaction with-fibulin-1 was assessed by GST-pull down assay. MCF-7 cell growth and gene expression, after fibulin-1 silencing by siRNA, were evaluated. Finally, the expression of fibulin-1 was correlated to clinical and pathological data of 21 breast cancer tissue samples.Key findingsFibulin-1 was expressed in both cell lines and it was increased by estradiol. SHBG interacted with fibulin-1C; proteins co-localized at MCF-7 cell membranes and SHBG localization at membranes disappeared after silencing fibulin-1. Fibulin-1 silencing, moreover, generated MCF-7 cells unresponsive to estradiol and SHBG and characterized by increased proliferation. Finally, in breast cancer tissue samples expressing fibulin-1 the proliferation index was significantly lower than in fibulin-1 negative samples.SignificanceFibulin-1 interacts with SHBG, it is associated with a less aggressive behavior of breast cancer cells and correlates to a better prognosis of the tumor.
Source: Life Sciences - Category: Biology Source Type: research

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Publication date: Available online 23 May 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Andrei Sakai, Ana Paula de Sousa Mesquista, Erica Valadares de Castro Levatti, Anita Hilda Straus, Helena B. Nader, Carla Cristina Lopes, Luciano CaseliAbstractTrastuzumab (Tmab) is a monoclonal antibody administered as targeted therapy for HER2-positive breast cancer whose molecular interactions at the HER2 receptor microenvironment are not completely clarified yet. This paper describes the influence of Tmab in the molecular organization of films of biological-relevant molecules at the air water interface. Fo...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - Category: Biochemistry Source Type: research
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Source: Lymphology - Category: Internal Medicine Tags: Lymphology Source Type: research
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Source: Lymphology - Category: Internal Medicine Tags: Lymphology Source Type: research
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Source: Medscape Medical News Headlines - Category: Consumer Health News Tags: Hematology-Oncology News Alert Source Type: news
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Source: Journal of Geriatric Oncology - Category: Cancer & Oncology Source Type: research
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Source: Reuters: Health - Category: Consumer Health News Tags: healthNews Source Type: news
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Source: Medscape Medical News Headlines - Category: Consumer Health News Tags: Hematology-Oncology News Source Type: news
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Source: IRBM - Category: Biomedical Engineering Source Type: research
Condition:   Estrogen Receptor-positive Breast Cancer Interventions:   Dietary Supplement: 2-Week Ketogenic Diet;   Drug: Letrozole Sponsor:   Vanderbilt-Ingram Cancer Center Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
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Source: European Review for Medical and Pharmacological Sciences - Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research
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