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Specialty: Parasitology

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Total 22 results found since Jan 2013.

Knocking down the expression of the molecular motors, myosin A, C and F genes in Toxoplasma gondii to decrease the parasite virulence
In conclusion, myosin proteins knock down proposes a promising therapeutic strategy to combat toxoplasmosis.PMID:37331576 | DOI:10.1016/j.exppara.2023.108565
Source: Experimental Parasitology - June 18, 2023 Category: Parasitology Authors: Tayebeh Zohrabi Mojtaba Azimi-Resketi Fereshteh Talaei Maryam Yaghoubi Mohamad Reza Ganjalikhany Farzaneh Mohamadi Farsani Abbasali Eskandarian Source Type: research

Comparative proteomic profiles of Schistosoma japonicum male worms derived from single-sex and bisexual infections
In this study, the proteomic profiles of single-sex infected male (SM) worms and bisexual infected mated male (MM) worms of Schistosoma japonicum at 18, 21, 23, and 25 days p.i. were identified through data-independent acquisition. In total, 674 differentially expressed proteins (DEPs) were identified for the SM and MM worms at all four timepoints. Bioinformatic analysis demonstrated that most of the DEPs were involved in biosynthetic processes including locomotion, cell growth and death, cell motility, and metabolic processes such as protein metabolism and glucose metabolism. Schistosoma japonicum glycosyltransferase (SjG...
Source: International Journal for Parasitology - October 20, 2022 Category: Parasitology Authors: Haoran Zhong Luobin Wu Yuqi Ren Fanglin Qin Yamei Jin Source Type: research

Schistosoma japonicum translationally controlled tumor protein, which is associated with the development of female worms, as a target for control of schistosomiasis
In this study, the proteomic profiles of single-sex infected female (SF) worms and bisexual infected mature female (MF) worms of Schistosoma japonicum at 18, 21, 23 and 25 days p.i. were identified with isobaric tags for relative quantitation-coupled liquid chromatography-tandem mass spectrometry. Differentially expressed proteins (DEPs) were subsequently used for bioinformatic analysis. Six highly expressed DEPs in MF worms were selected and long-term interference with small interfering RNA (siRNA) was conducted to determine biological functions. SiRNA against S. japonicum translationally controlled tumor protein (SjTCTP)...
Source: International Journal for Parasitology - March 23, 2022 Category: Parasitology Authors: Haoran Zhong Yuqi Ren Fanglin Qin Xiaochun Li Ling Hou Shaopeng Gu Yamei Jin Source Type: research

In vitro effects of Harmine against Echinococcus granulosus protoscoleces by stimulating DNA damage
In this study, we aim to evaluate the anti-parasitic efficacy and the mechanism of DNA damage of HM against E. granulosus. In vitro, the results indicated that, within two and three days of treatment, ABZ killed 30.4% and 35.3% of protoscoleces, whereas HM killed 52.7% and 100% of protoscoleces, respectively. Furthermore, the presence of abnormalities in the internal structure of protoscoleces was examined by ultrastructural images of TEM, and the result showed that there were scattered nucleoli and heterochromatin margination phenomenon by HM treatment. DNA damage of protoscoleces was examined by using the comet assay, an...
Source: Experimental Parasitology - June 7, 2021 Category: Parasitology Authors: Shuai Lu Limei Wen Yuehong Gong Chunyan Tian Huijing Gao Bei Chen Guodong L ü Jun Zhao Jianhua Wang Source Type: research

In vitro effects of harmine against Echinococcus granulosus protoscoleces by stimulating DNA damage
In this study, we aim to evaluate the anti-parasitic efficacy and the mechanism of DNA damage of HM against E. granulosus. In vitro, the results indicated that, within two and three days of treatment, ABZ killed 30.4% and 35.3% of protoscoleces, whereas HM killed 52.7% and 100% of protoscoleces, respectively. Furthermore, the presence of abnormalities in the internal structure of protoscoleces was examined by ultrastructural images of TEM, and the result showed that there were scattered nucleoli and heterochromatin margination phenomenon by HM treatment. DNA damage of protoscoleces was examined by using the comet assay, an...
Source: Experimental Parasitology - June 7, 2021 Category: Parasitology Authors: Shuai Lu Limei Wen Yuehong Gong Chunyan Tian Huijing Gao Bei Chen Guodong L ü Jun Zhao Jianhua Wang Source Type: research

Schistosoma mansoni venom allergen-like protein 6 (SmVAL6) maintains tegumental barrier function.
Abstract The Schistosoma mansoni venom allergen-like protein (SmVAL) superfamily is a collection of at least 29 molecules that have been classified into two distinctive groups (Group 1 and Group 2 SmVALs). The fundamental basis for SmVAL segregation relates to signal peptide and conserved cysteine retention (present in all Group 1 SmVALs, but absent in all Group 2 SmVALs). These structural differences have led to the hypothesis that most Group 1 SmVALs, found as components of schistosome excretory/secretory (E/S) products, predominantly interact with their environment (intermediate or definitive hosts) whereas the...
Source: International Journal for Parasitology - November 27, 2020 Category: Parasitology Authors: Perally S, Geyer KK, Farani PSG, Chalmers IW, Fernandez-Fuentes N, Maskell DR, Hulme BJ, Forde-Thomas J, Phillips D, Farias LP, Collins JJ, Hoffmann KF Tags: Int J Parasitol Source Type: research

Adenosine A3 Receptor Mediates ERK1/2- and JNK-Dependent TNF- α Production in Toxoplasma gondii-Infected HTR8/SVneo Human Extravillous Trophoblast Cells.
Adenosine A3 Receptor Mediates ERK1/2- and JNK-Dependent TNF-α Production in Toxoplasma gondii-Infected HTR8/SVneo Human Extravillous Trophoblast Cells. Korean J Parasitol. 2020 Aug;58(4):393-402 Authors: Ye W, Sun J, Li C, Fan X, Gong F, Huang X, Deng M, Chu JQ Abstract Toxoplasma gondii is an intracellular parasite that causes severe disease when the infection occurs during pregnancy. Adenosine is a purine nucleoside involved in numerous physiological processes; however, the role of adenosine receptors in T. gondii-induced trophoblast cell function has not been investigated until now. The goal of th...
Source: Korean Journal of Parasitology - September 3, 2020 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Immunomodulator mediated changes in plasma membrane calcium ATPase in controlling visceral leishmaniasis.
Abstract Immunomodulation is an emerging concept to combat infection in recent years. Immunomodulators like arabinosylated-lipoarabinomannan (Ara-LAM) and glycyrrhizic-acid (GA) possess anti-leishmanial property, whereas sodium-antimony-gluconate (SAG) is still considered as the first choice for chemotherapy against leishmaniasis. During infection, invasion of Leishmania donovani needs the potential requirement of Ca2+, which is further responsible for apoptosis in intracellular amastigotes. However, suppression of elevated intracellular calcium by the activation of plasma-membrane-calcium-ATPase (PMCA4) facilitat...
Source: Experimental Parasitology - July 18, 2020 Category: Parasitology Authors: Roy N, Ghosh S, Juin SK, Ghosh R, Majumdar SB, Majumdar S Tags: Exp Parasitol Source Type: research

Development of siRNA mediated RNA interference and functional analysis of novel parasitic nematode-specific protein of Setaria digitata.
In this study, we developed an effective siRNA delivery method by microinjection and utilized the siRNAi tool to knockdown SDNP to study the phenotypic and cellular changes associated with the interference. qPCR analysis revealed, a significant reduction of SDNP transcript levels following siRNA microinjection into S. digitata adult worms. Similarly, immunohistochemical staining indicated a reduction of SDNP protein expression. Furthermore, worms treated with siRNA showed a significant reduction of microfilariae release together with embryonic lethality by arresting an early developmental stage compared to non-treated worm...
Source: Experimental Parasitology - February 12, 2018 Category: Parasitology Authors: Somarathne MBCL, Gunawardena YINS, Chandrasekharan NV, Dassanayake RS Tags: Exp Parasitol Source Type: research

Identification and Characterization of Protein Arginine Methyltransferase 1 in Acanthamoeba castellanii.
In this study, the full-length 1,059 bp cDNA sequence of Acanthamoeba castellanii PRMT1 (AcPRMT1) was cloned for the first time. The AcPRMT1 protein comprised of 352 amino acids with a SAM-dependent methyltransferase PRMT-type domain. The expression level of AcPRMT1 was highly increased during encystation of A. castellanii. The EGFP-AcPRMT1 fusion protein was distributed over the cytoplasm, but it was mainly localized in the nucleus of Acanthamoeba. Knock down of AcPRMT1 by synthetic siRNA with a complementary sequence failed to form mature cysts. These findings suggested that AcPRMT1 plays a critical role in the regulatio...
Source: Korean Journal of Parasitology - May 17, 2017 Category: Parasitology Tags: Korean J Parasitol Source Type: research