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Specialty: Dermatology
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Total 964 results found since Jan 2013.
334 Invalidation of ADAR1 in human keratinocytes unravels its contribution to epithelial cell death and immunity
Adenosine Deaminase Acting on RNA 1 (ADAR1) is a dsRNA A/I editing enzyme which loss-of-function (LOF) mutations account for monogenic disorders with skin manifestations called interferonopathies. However the role of ADAR1 in primary human keratinocytes (HK) remains unknown. This work aims to assess ADAR1 functions in HK homeostasis in vitro. ADAR1 knock-out (KO) or knocked-down (KD) HK were generated respectively by crispr-cas9 and by siRNA. Viability (Trypan blue), KO score (ICE analysis) and mRNA and protein expression of several.
Source: Journal of Investigative Dermatology - November 18, 2022 Category: Dermatology Authors: F. Assan, A. Izmiryan, M. Fremond, H. Bachelez Source Type: research
374 Eukaryotic translation initiation factor 4E (eIF4E) as a target of anti-psoriatic treatment
Eukaryotic translation initiation factor 4E (eIF4E) has been known to play a critical role in the regulation of gene expression in eukaryotes and to affect many essential cellular processes, including proliferation, apoptosis and differentiation. We and others found eIF4E and other eIF subunits upregulated in human psoriatic skin and thus hypothesized that they are involved in the pathophysiology of psoriasis and can serve as direct topical targets for anti-psoriatic treatment. We used specific eIF4E siRNA or briciclib an eIF4E inhibitor in HaCaT cells, a human 3D-psoriasis tissue model as well as the imiquimod and TGF ß ...
Source: Journal of Investigative Dermatology - November 18, 2022 Category: Dermatology Authors: N. Golob-Schwarzl, J. Pilic, T. Benezeder, N. Bordag, C. Painsi, P. Wolf Source Type: research
The paranuclear dot in Merkel cell carcinoma, how does it form and what does it do?
Background and aims: Staining for Cytokeratin 20 (CK20) and other intermediate filaments in a paranuclear dot pattern is a histological feature used to diagnose the neuroendocrine skin cancer Merkel cell carcinoma (MCC). The functional significance of these paranuclear protein aggregates is unknown. Therefore, we sought to investigate the structure and function of the paranuclear dot in MCC. Methods: In native tumors and MCC cell lines we used: immunostaning, microscopy, chemical inhibition, siRNA knockdown, and cell death modulators to study the paranuclear dot.
Source: Journal of Investigative Dermatology - September 21, 2022 Category: Dermatology Authors: Natasha T. Hill, Loren Collado, Tyler Kellenberger, Kunio Nagashima, Serena Vilasi, Paul W. Harms, Isaac Brownell Source Type: research
652 EPAC-mTORC1 signaling regulates proliferation of primary melanoma cells and loss of dependence on EPAC signaling correlates with melanoma progression
In this study, we show that pharmacological inhibition or siRNA-mediated knockdown of EPAC selectively inhibits the growth and survival of primary melanoma cells by downregulation of cell cycle proteins and inhibiting the cell cycle progression independent of ERK1/2 phosphorylation.
Source: Journal of Investigative Dermatology - July 20, 2022 Category: Dermatology Authors: M.K. Singh, A. Krishnan, A.I. Bhasker, C.I. Rodriguez, E. Castro-Perez, M. Ndiaye, N. Ahmad, H. Khan, S.M. Schieke, V. Setaluri Source Type: research
622 Regulation of XPC binding dynamics and global nucleotide excision repair by p63 and vitamin D receptor
p63 and the vitamin D receptor (VDR) play important roles in epidermal development and differentiation, but their roles and relationship in the response to ultraviolet (UV) radiation are unclear. Using TERT-immortalized human keratinocytes expressing shRNA targeting p63 in concert with exogenously applied siRNA targeting VDR, we assessed p63 and VDR ’s separate and combined effect on nucleotide excision repair (NER) of UV-induced 6-4 photoproducts (6-4PP) with a focus on the XPC DNA damage recognition protein.
Source: Journal of Investigative Dermatology - July 20, 2022 Category: Dermatology Authors: C. Wong, D.H. Oh Source Type: research
Effects of & lt;10- µm Particulate Matter on Cultured Human Sebocytes and Outer Root Sheath Cells and Usefulness of Siegesbeckia Herba Extract
CONCLUSION: Effects of PM10 on cultured human sebocytes and ORS cells can be regulated by SH.PMID:35721334 | PMC:PMC9171181 | DOI:10.5021/ad.2022.34.3.163
Source: Annals of Dermatology - June 20, 2022 Category: Dermatology Authors: Mi Hee Kwack Nam Gyoung Ha Weon Ju Lee Source Type: research
