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1063 Topically applied NanoFlares to measure gene expression in vivo: Proof-of-concept
Spherical nucleic acids (SNAs) are topical agents that penetrate human skin to knock down genes via antisense and siRNA pathways. NanoFlares, SNAs that release sequence-specific fluorescence when binding to intracellular mRNA targets, measure mRNA levels in live cells. We hypothesized that NanoFlares could be topically applied to measure gene expression in vivo. As proof-of-concept, we detected connective tissue growth factor (CTGF), known to be increased in hypertrophic scars and keloids. Using fibroblasts from normal skin, keloids, hypertrophic scars and peri-scar tissue, we found that: a) NanoFlares are specific; b) exp...
Source: Journal of Investigative Dermatology - April 27, 2018 Category: Dermatology Authors: A.S. Paller, D. Yeo, C. Wiraja, C. Mirkin, C. Xu Tags: Pharmacology and Drug Development Source Type: research

Transforming growth factor-beta inducible early gene-1 (TIEG1) represses Smad7-mediated activation of TGF- β1/Smad signaling in keloid pathogenesis
Transforming growth factor β (TGF-β)/Smad signaling plays a key role in excessive fibrosis and keloid formations. Smad7 is a negative feedback regulator that prevents activation of TGF-β/Smad signaling. However, the regulatory mechanism for Smad7 in the keloid pathogenic process remains elusive. Here, we show that expressi on of TGF-β inducible early gene-1 (TIEG1) is markedly higher in keloid fibroblasts (KFs), while protein, mRNA, and promoter activity levels of Smad7 are decreased. When TIEG1 was knocked down with small interfering RNA (siRNA), both the promoter activity and protein expression of Smad7 were increa s...
Source: Journal of Investigative Dermatology - January 16, 2017 Category: Dermatology Authors: Zhi-Cheng Hu, Fen Shi, Peng Liu, Jian Zhang, Dong Guo, Xiao-ling Cao, Chu-Fen Chen, Shanqiang Qu, Jia-Yuan Zhu, Bing Tang Tags: Original Article Source Type: research

A Role for Neuregulin-1 in Promoting Keloid Fibroblast Migration via ErbB2-mediated Signaling.
Abstract Keloid disease is a fibroproliferative tumour characterised by aggressive local invasion, evident from a clinically and histologically active migrating margin. During combined laser capture microdissection and microarray analysis-based in situ gene expression profiling, we identified upregulation of the polypeptide growth factor neuregulin-1 (NRG1) and ErbB2 oncogene in keloid margin dermis, leading to the hypothesis that NRG1 contributed to keloid margin migration through ErbB2-mediated signalling. The aim of this study was to probe this hypothesis through functional in vitro studies. Exogenous NRG1 addi...
Source: Acta Dermato-Venereologica - November 23, 2016 Category: Dermatology Authors: Jumper N, Hodgkinson T, Paus R, Bayat A Tags: Acta Derm Venereol Source Type: research

miR-21 promotes collagen production in keloid via Smad7
Conclusions miR-21 promoted collagen production in keloid by negatively regulating the expression of the Smad7.
Source: Burns - October 4, 2016 Category: Dermatology Source Type: research

Oxidative Damage and Nuclear Factor Erythroid 2-Related Factor 2 Protein Expression in Normal Skin and Keloid Tissue.
CONCLUSION: Collectively, our data indicate that Nrf2 expression is downregulated in keloid tissues, and that Nrf2 is involved in the development of apoptosis in Nrf2 siRNA-transfected fibroblasts. We propose that a defective antioxidant system and apoptotic dysregulation may participate in keloid pathogenesis. PMID: 26512164 [PubMed]
Source: Annals of Dermatology - October 31, 2015 Category: Dermatology Tags: Ann Dermatol Source Type: research

2ME2 increase radiation‐induced apoptosis of keloid fibroblasts by targeting HIF‐1α in vitro
ConclusionsThe present study indicates that HIF‐1α might serve as a therapeutic target for keloids. Furthermore, suppression of HIF‐1α by 2ME2 may be a promising therapeutic adjuvant in radiation therapy for keloids.
Source: Australasian Journal of Dermatology - April 15, 2015 Category: Dermatology Authors: Fei Long, Loubin Si, Xiao Long, Bob Yang, Xiaojun Wang, Fuquan Zhang Tags: Original Research Source Type: research

2ME2 increase radiation ‐induced apoptosis of keloid fibroblasts by targeting HIF‐1α in vitro
ConclusionsThe present study indicates that HIF‐1α might serve as a therapeutic target for keloids. Furthermore, suppression of HIF‐1α by 2ME2 may be a promising therapeutic adjuvant in radiation therapy for keloids.
Source: Australasian Journal of Dermatology - April 14, 2015 Category: Dermatology Authors: Fei Long, Loubin Si, Xiao Long, Bob Yang, Xiaojun Wang, Fuquan Zhang Tags: Original Research Source Type: research

NADPH oxidase‐2 is a key regulator of human dermal fibroblasts: a potential therapeutic strategy for the treatment of skin fibrosis
This article is protected by copyright. All rights reserved.
Source: Experimental Dermatology - June 30, 2014 Category: Dermatology Authors: Guo‐You Zhang, Liang‐Cai Wu, Tao Dai, Shi‐Yi Chen, An‐Yuan Wang, Kang Lin, Da‐Mu Lin, Jing‐Quan Yang, Biao Cheng, Li Zhang, Wei‐Yang Gao, Zhi‐Jie Li Tags: Regular Article Source Type: research