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Infectious Disease: Outbreaks

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Total 80 results found since Jan 2013.

Designing an effective therapeutic siRNA to silence RdRp gene of SARS-CoV-2
Infect Genet Evol. 2021 Jun 2:104951. doi: 10.1016/j.meegid.2021.104951. Online ahead of print.ABSTRACTThe devastating outbreak of COVID-19 has spread all over the world and has become a global health concern. There is no specific therapeutics to encounter the COVID-19. Small interfering RNA (siRNA)-based therapy is an efficient strategy to control human viral infections employing post-transcriptional gene silencing (PTGS) through neutralizing target complementary mRNA. RNA-dependent RNA polymerase (RdRp) encoded by the viral RdRp gene as a part of the replication-transcription complex can be adopted as an acceptable targe...
Source: Infection, Genetics and Evolution - June 5, 2021 Category: Genetics & Stem Cells Authors: Mohammad Mahfuz Ali Khan Shawan Ashish Ranjan Sharma Manojit Bhattacharya Bidyut Mallik Farhana Akhter Md Salman Shakil Md Mozammel Hossain Subrata Banik Sang-Soo Lee Md Ashraful Hasan Chiranjib Chakraborty Source Type: research

Designing potential siRNA molecule for the nucleocapsid(N) gene silencing of different SARS-CoV-2 strains of Bangladesh: Computational approach
In this study, we attempted to design a siRNA molecule to silence the most suitable nucleocapsid(N) gene of SARS-CoV-2, which play a major role during viral pathogenesis, replication, encapsidation and RNA packaging. At first, 270 complete N gene sequences of different strains in Bangladesh of these viruses were retrieved from the NCBI database. Different computational methods were used to design siRNA molecules. A siRNA molecule was built against these strains using the SiDirect 2.0 server. Using Mfold and the OligoCalc server, the siRNA molecule was tested for its secondary structure and GC material. The Clustal Omega to...
Source: Computational Biology and Chemistry - May 13, 2021 Category: Bioinformatics Authors: Syed Shahariar Bappy Abu Zaffar Shibly Sorna Sultana A K M Mohiuddin Yearul Kabir Source Type: research

Subretinal transfection of chitosan-loaded TLR3-siRNA for the treatment of experimental autoimmune uveitis.
Abstract The local interaction between retinal pigment epithelium (RPE) and immigrated effector T cells is crucial for the pathogenesis of autoimmune uveitis. After being activated by the pattern recognition receptors (PRRs) signaling pathway, RPE can present the antigen reactivated invading autoreactive T cells, resulting in uveitis. In the present study, we showed that the transfection of chitosan-loaded TLR3-siRNA toward RPE could effectively remit experimental autoimmune uveitis (EAU) in B10RIII mice. Initially, we verified the constitutive expression of Tlr3 in RPE at high levels, which was not altered in the...
Source: European Journal of Pharmaceutics and Biopharmaceutics - September 17, 2013 Category: Drugs & Pharmacology Authors: Chen S, Yan H, Sun B, Zuo A, Liang D Tags: Eur J Pharm Biopharm Source Type: research

A computational approach to design potential siRNA molecules as a prospective tool for silencing nucleocapsid phosphoprotein and surface glycoprotein gene of SARS-CoV-2.
Abstract An outbreak, caused by an RNA virus, SARS-CoV-2 named COVID-19 has become pandemic with a magnitude which is daunting to all public health institutions in the absence of specific antiviral treatment. Surface glycoprotein and nucleocapsid phosphoprotein are two important proteins of this virus facilitating its entry into host cell and genome replication. Small interfering RNA (siRNA) is a prospective tool of the RNA interference (RNAi) pathway for the control of human viral infections by suppressing viral gene expression through hybridization and neutralization of target complementary mRNA. So, in this stu...
Source: Genomics - December 12, 2020 Category: Genetics & Stem Cells Authors: Chowdhury UF, Shohan MUS, Hoque KI, Beg MA, Siam MKS, Moni MA Tags: Genomics Source Type: research

Lipid nanoparticle siRNA treatment of Ebola-virus-Makona-infected nonhuman primates
n & Thomas W. Geisbert The current outbreak of Ebola virus in West Africa is unprecedented, causing more cases and fatalities than all previous outbreaks combined, and has yet to be controlled. Several post-exposure interventions have been employed under compassionate use to treat patients repatriated to Europe and the United States. However, the in vivo efficacy of these interventions against the new outbreak strain of Ebola virus is unknown. Here we show that lipid-nanoparticle-encapsulated short interfering RNAs (siRNAs) rapidly adapted to target the Makona outbreak strain of Ebola virus are able to protect 100...
Source: Nature - April 22, 2015 Category: Research Authors: Emily P. ThiChad E. MireAmy C. H. LeeJoan B. GeisbertJoy Z. ZhouKrystle N. AgansNicholas M. SneadDaniel J. DeerTrisha R. BarnardKarla A. FentonIan MacLachlanThomas W. Geisbert Tags: Letter Source Type: research

Difference in the lipid nanoparticle technology employed in three approved siRNA (Patisiran) and mRNA (COVID-19 vaccine) drugs
Drug Metab Pharmacokinet. 2021 Oct 10;41:100424. doi: 10.1016/j.dmpk.2021.100424. Online ahead of print.ABSTRACTNucleic acid therapeutics are developing into precise medicines that can manipulate specific genes. However, the development of safe and effective delivery system for the target cells has remained a challenge. Lipid nanoparticles (LNPs) have provided a revolutionary delivery system that can ensure multiple clinical translation of RNA-based candidates. In 2018, Patisiran (Onpattro) was first approved as an LNP-based siRNA drug. In 2020, during the coronavirus disease 2019 (COVID-19) outbreak, LNPs have enabled the...
Source: Drug Metabolism and Pharmacokinetics - November 10, 2021 Category: Drugs & Pharmacology Authors: Yuta Suzuki Hiroshi Ishihara Source Type: research