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Transfection efficacy and drug release depends upon the PEG derivative in cationic lipoplexes: Evaluation in 3D in vitro model and in vivo
J Biomed Mater Res B Appl Biomater. 2023 May 3. doi: 10.1002/jbm.b.35259. Online ahead of print.ABSTRACTThe goal of the study was to estimate transfection efficacy and drug release in function of the PEG derivative in cationic liposomes and lipoplexes in both 2D and 3D in vitro models as well as in a mouse model (in vivo). For this purpose, cationic PEGylated nanocarriers based on OrnOrnGlu(C16 H33 )2 lipopeptides were fabricated and characterized. The nanocarriers were loaded with DNA plasmid pGL3 or with siRNA targeting 5'-UTR region of Hepatitis C virus, and their transfection efficacies were studied by luciferase test ...
Source: Biomed Res - May 3, 2023 Category: Research Authors: Gileva A M Koloskova O O Nosova A S Vishniakova L I Simonova V A Kurbanova L A Egorenkov E A Smirnov V V Budanova U A Sebyakin Yu L Suzina N E Khaitov M R Markvicheva E Source Type: research

siRNA- and miRNA-Based Therapeutics for Liver Fibrosis
Liver fibrosis is a wound-healing process induced by chronic liver injuries, such as nonalcoholic steatohepatitis, hepatitis, alcohol abuse, and metal poisoning. The accumulation of excessive extracellular matrix (ECM) in the liver is a key characteristic of liver fibrosis. Activated hepatic stellate cells (HSCs) are the major producers of ECM and therefore play irreplaceably important roles during the progression of liver fibrosis. Liver fibrogenesis is highly correlated with the activation of HSCs, which is regulated by numerous profibrotic cytokines.
Source: Translational Research - August 12, 2019 Category: Research Authors: Zhen Zhao, Chien-Yu Lin, Kun Cheng Tags: Review Article Source Type: research

URG11 Regulates Prostate Cancer Cell Proliferation, Migration, and Invasion.
Abstract Upregulated gene 11 (URG11), a new gene upregulated by hepatitis B virus X protein, is involved in the development and progression of several tumors, including liver, stomach, lung, and colon cancers. However, the role of URG11 in prostate cancer remains yet to be elucidated. By determined expression in human prostate cancer tissues, URG11 was found significantly upregulated and positively correlated with the severity of prostate cancer, compared with that in benign prostatic hyperplasia tissues. Further, the mRNA and protein levels of URG11 were significantly upregulated in human prostate cancer cell lin...
Source: Biomed Res - June 30, 2018 Category: Research Authors: Pan B, Ye Y, Liu H, Zhen J, Zhou H, Li Y, Qu L, Wu Y, Zeng C, Zhong W Tags: Biomed Res Int Source Type: research

Investigating the antiviral role of cell death‐inducing DFF45‐like effector B in HCV replication
This article is protected by copyright. All rights reserved.
Source: FEBS Journal - June 1, 2014 Category: Research Authors: Ragunath Singaravelu, Julie Delcorde, Rodney K Lyn, Rineke H Steenbergen, Daniel M Jones, D Lorne Tyrrell, Rodney S Russell, John Paul Pezacki Tags: Original Article Source Type: research

Programmable folding of fusion RNA in vivo and in vitro driven by pRNA 3WJ motif of phi29 DNA packaging motor
Misfolding and associated loss of function are common problems in constructing fusion RNA complexes due to changes in energy landscape and the nearest-neighbor principle. Here we report the incorporation and application of the pRNA-3WJ motif of the phi29 DNA packaging motor into fusion RNA with controllable and predictable folding. The motif included three discontinuous ~18 nucleotide (nt) fragments, displayed a distinct low folding energy (Shu D et al., Nature Nanotechnology, 2011, 6:658–667), and folded spontaneously into a leading core that enabled the correct folding of other functionalities fused to the RNA comp...
Source: Nucleic Acids Research - January 26, 2014 Category: Research Authors: Shu, D., Khisamutdinov, E. F., Zhang, L., Guo, P. Tags: RNA characterisation and manipulation Methods Online Source Type: research

A Novel System for Producing Infectious Hepatitis C Virus (HCV) Virions and Development of a Novel Reporter System for Studying HCV Entry
HCV has infected an estimated 3% of the world population in whom viral infection persists for more than two third of the cases, often resulting in life-threatening complications. The standard of care (pegylated interferon alpha-2 plus ribavirin) is efficient in only 50% of treated patients, costly and has numerous side effects. In addition, viral resistance to newly developed drugs -- targeting viral protease or RNA polymerase -- has been described, but no vaccine is yet available. The difficulty in developing HCV vaccines is largely due to the broad sequence-diversity displayed by HCV, the frequent occurrence of viral mut...
Source: NIH OTT Licensing Opportunities - June 23, 2009 Category: Research Authors: ott-admin Source Type: research

A Novel Flavivirus-based System For Production Of Hepatitis C Virus (HCV): Production Of Infectious Virions And Development Of A Novel Reporter System For Studying HCV Virion Entry.
A Novel System for Producing Infectious Hepatitis C Virus (HCV) Virions and Development of a Novel Reporter System for Studying HCV EntryDescription of Technology: HCV has infected an estimated 3% of the world population in whom viral infection persists for more than two third of the cases, often resulting in life-threatening complications. The standard of care (pegylated interferon alpha-2 plus ribavirin) is efficient in only 50% of treated patients, costly and has numerous side effects. In addition, viral resistance to newly developed drugs -- targeting viral protease or RNA polymerase -- has been described, but no vacci...
Source: NIH OTT Licensing Opportunities - June 23, 2009 Category: Research Authors: ott-admin Source Type: research