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Source: Theranostics
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Total 110 results found since Jan 2013.
Interleukin 3- receptor targeted exosomes inhibit in vitro and in vivo Chronic Myelogenous Leukemia cell growth
In this study, we engineered HEK293T cells to express the exosomal protein Lamp2b, fused to a fragment of Interleukin 3 (IL3). The IL3 receptor (IL3-R) is overexpressed in CML blasts compared to normal hematopoietic cells and thus is able to act as a receptor target in a cancer drug delivery system. Here we show that IL3L exosomes, loaded with Imatinib or with BCR-ABL siRNA, are able to target CML cells and inhibit in vitro and in vivo cancer cell growth.
Source: Theranostics - September 12, 2017 Category: Molecular Biology Authors: Daniele Bellavia, Stefania Raimondo, Giovanna Calabrese, Stefano Forte, Marta Cristaldi, Agostina Patinella, Lorenzo Memeo, Mauro Manno, Samuele Raccosta, Patrizia Diana, Girolamo Cirrincione, Gianluca Giavaresi, Francesca Monteleone, Simona Fontana, Giac Tags: Research Paper Source Type: research
Pharmacokinetic Behaviors of Intravenously Administered siRNA in Glandular Tissues
In this study, we comprehensively investigated the behavior of siRNAs in vivo in various tissues and demonstrated that intravenously-injected naked siRNA accumulated remarkably in the submandibular gland, bulbourethral gland, and pancreas, with a respective half-life of ~22.7, ~45.6, and ~30.3 h. This was further confirmed by gel separation of tissue homogenates and/or supernatants. In vivo imaging and cryosectioning suggested that delivery carriers significantly influence the distribution and elimination profiles of siRNA. Gene-silencing assays revealed that neither naked nor liposome-formulated siRNA resul...
Source: Theranostics - June 26, 2016 Category: Molecular Biology Authors: Yuanyu Huang, Qiang Cheng, Jia-Li Ji, Shuquan Zheng, Lili Du, Lingwei Meng, Yidi Wu, Deyao Zhao, Xiaoxia Wang, Li Lai, Huiqing Cao, Kai Xiao, Shan Gao, Zicai Liang Tags: Research Paper Source Type: research
Dihydropyrimidine Dehydrogenase Is a Prognostic Marker for Mesenchymal Stem Cell-Mediated Cytosine Deaminase Gene and 5-Fluorocytosine Prodrug Therapy for the Treatment of Recurrent Gliomas
We investigated a therapeutic strategy for recurrent malignant gliomas using mesenchymal stem cells (MSC), expressing cytosine deaminase (CD), and prodrug 5-Fluorocytosine (5-FC) as a more specific and less toxic option. MSCs are emerging as a novel cell therapeutic agent with a cancer-targeting property, and CD is considered a promising enzyme in cancer gene therapy which can convert non-toxic 5-FC to toxic 5-Fluorouracil (5-FU). Therefore, use of prodrug 5-FC can minimize normal cell toxicity. Analyses of microarrays revealed that targeting DNA damage and its repair is a selectable option for gliomas after the standard c...
Source: Theranostics - June 26, 2016 Category: Molecular Biology Authors: Taemoon Chung, Juri Na, Young-il Kim, Da-Young Chang, Young Il Kim, Hyeonjin Kim, Ho Eun Moon, Keon Wook Kang, Dong Soo Lee, June-Key Chung, Sung-Soo Kim, Haeyoung Suh-Kim, Sun Ha Paek, Hyewon Youn Tags: Research Paper Source Type: research
In vivo MR and Fluorescence Dual-modality Imaging of Atherosclerosis Characteristics in Mice Using Profilin-1 Targeted Magnetic Nanoparticles
Conclusion: PC-NPs is a promising dual modality imaging probe, which may improve molecular diagnosis of plaque characteristics and evaluation of pharmaceutical interventions for atherosclerosis.
Source: Theranostics - June 5, 2016 Category: Molecular Biology Authors: Yabin Wang, Jiangwei Chen, Bo Yang, Hongyu Qiao, Lei Gao, Tao Su, Sai Ma, Xiaotian Zhang, Xiujuan Li, Gang Liu, Jianbo Cao, Xiaoyuan Chen, Yundai Chen, Feng Cao Tags: Research Paper Source Type: research
P-glycoprotein Mediates Postoperative Peritoneal Adhesion Formation by Enhancing Phosphorylation of the Chloride Channel-3
P-glycoprotein (P-gp) is encoded by the multidrug resistance (MDR1) gene and is well studied as a multi-drug resistance transporter. Peritoneal adhesion formation following abdominal surgery remains an important clinical problem. Here, we found that P-gp was highly expressed in human adhesion fibroblasts and promoted peritoneal adhesion formation in a rodent model. Knockdown of P-gp expression by intraperitoneal injection of MDR1-targeted siRNA significantly reduced both the peritoneal adhesion development rate and adhesion grades. Additionally, we found that operative injury up-regulated P-gp expression in peritoneal fibr...
Source: Theranostics - June 5, 2016 Category: Molecular Biology Authors: Lulu Deng, Qin Li, Guixian Lin, Dan Huang, Xuxin Zeng, Xinwei Wang, Ping Li, Xiaobao Jin, Haifeng Zhang, Chunmei Li, Lixin Chen, Liwei Wang, Shulin Huang, Hongwei Shao, Bin Xu, Jianwen Mao Tags: Research Paper Source Type: research
Mono-arginine Cholesterol-based Small Lipid Nanoparticles as a Systemic siRNA Delivery Platform for Effective Cancer Therapy
This study describes the development of neutral, small lipid nanoparticles (SLNPs) made of a nontoxic cationic cholesterol derivative, as a suitable carrier of systemic siRNA to treat cancers. The cationic cholesterol derivative, mono arginine-cholesterol (MA-Chol), was synthesized by directly attaching an arginine moiety to cholesterol via a cleavable ester bond. siRNA-loaded SLNPs (siRNA@SLNPs) were prepared using MA-Chol and a neutral helper lipid, dioleoyl phosphatidylethanolamine (DOPE), as major components and a small amount of PEGylated phospholipid mixed with siRNA. The resulting nanoparticles were less than ~...
Source: Theranostics - June 5, 2016 Category: Molecular Biology Authors: Jinju Lee, Phei Er Saw, Vipul Gujrati, Yonghyun Lee, Hyungjun Kim, Sukmo Kang, Minsuk Choi, Jae-Il Kim, Sangyong Jon Tags: Research Paper Source Type: research
Triple-Layered pH-Responsive Micelleplexes Loaded with siRNA and Cisplatin Prodrug for NF-Kappa B Targeted Treatment of Metastatic Breast Cancer
The combination of chemotherapy and RNA interference is a promising approach for efficient cancer therapy. However, the success of such a strategy is hampered by the lack of suitable vectors to coordinate small interfering RNA (siRNA) and chemotherapeutic drug into one single platform. We herein report a novel triple-layered pH-responsive micelleplex loading siRNA and alkylated cisplatin prodrug for NF-Kappa B targeted treatment of metastatic breast cancer. The micelles were self-assembled from poly(ethylene glycol)-block-poly(aminolated glycidyl methacrylate)-block-poly(2-(diisopropyl amino) ethyl methacrylate) (PEG-b-PAG...
Source: Theranostics - June 5, 2016 Category: Molecular Biology Authors: Haijun Yu, Chengyue Guo, Bing Feng, Jianping Liu, Xianzhi Chen, Dangge Wang, Lesheng Teng, Youxin Li, Qi Yin, Zhiwen Zhang, Yaping Li Tags: Research Paper Source Type: research
ICAM-1-Targeted, Lcn2 siRNA-Encapsulating Liposomes are Potent Anti-angiogenic Agents for Triple Negative Breast Cancer
In this study, we have engineered a novel liposomal small interfering RNA (siRNA) delivery system to target triple negative breast cancer (TNBC) via a recently identified molecular target, intercellular adhesion molecule-1 (ICAM-1). This ICAM-1-targeted, Lcn2 siRNA- encapsulating liposome (ICAM-Lcn2-LP) binds human TNBC MDA-MB-231cells significantly stronger than non-neoplastic MCF-10A cells. Efficient Lcn2 knockdown by ICAM-Lcn2-LPs led to a significant reduction in the production of vascular endothelial growth factor (VEGF) from MDA-MB-231 cells, which, in turn, led to reduced angiogenesis both in vitro and in vivo. Angi...
Source: Theranostics - June 5, 2016 Category: Molecular Biology Authors: Peng Guo, Jiang Yang, Di Jia, Marsha A. Moses, Debra T. Auguste Tags: Research Paper Source Type: research
EpCAM Aptamer-mediated Survivin Silencing Sensitized Cancer Stem Cells to Doxorubicin in a Breast Cancer Model
Understanding the molecular basis of drug resistance and utilising this information to overcome chemoresistance remains a key challenge in oncology. Here we report that survivin, a key protein implicated in drug resistance, is overexpressed in cancer stem cell pool of doxorubicin-resistant breast cancer cells. Moreover, by utilising an active targeting system consisting of an RNA aptamer targeted against the epithelial cell adhesion molecule and a Dicer substrate survivin siRNA, we could deliver a high dose of the siRNA to cancer stem cells in xenograft tumours. Importantly, silencing of survivin with this aptamer-siRNA ch...
Source: Theranostics - October 20, 2015 Category: Molecular Biology Authors: Tao Wang, Michael P. Gantier, Dongxi Xiang, Andrew G Bean, Matthew Bruce, Shu-Feng Zhou, Mustafa Khasraw, Alister Ward, Li Wang, Ming Q. Wei, Hadi AlShamaileh, Lijue Chen, Xiaodong She, Jia Lin, Lingxue Kong, Sarah Shigdar, Wei Duan Tags: Research Paper Source Type: research
Photoluminescent Mesoporous Silicon Nanoparticles with siCCR2 Improve the Effects of Mesenchymal Stromal Cell Transplantation after Acute Myocardial Infarction
Conclusions: PMSNs-siCCR2-mediated CCR2 gene silencing in Ly6Chigh monocytes improved the effectiveness of MSC transplantation and selectively ameliorated myocardial remodeling after AMI. These results suggest that PMSNs-siCCR2 could potentially be used to develop an anti-inflammatory therapy for post-AMI MSC transplantation.
Source: Theranostics - September 18, 2015 Category: Molecular Biology Authors: Wenbin Lu, ZhuoYing Xie, Yong Tang, Ling Bai, Yuyu Yao, Cong Fu, Genshan Ma Tags: Research Paper Source Type: research
A Light-Driven Therapy of Pancreatic Adenocarcinoma Using Gold Nanorods-Based Nanocarriers for Co-Delivery of Doxorubicin and siRNA
In this work, we report the engineering of polyelectrolyte polymers coated Gold nanorods (AuNRs)-based nanocarriers that are capable of co-delivering small interfering RNA (siRNA) and an anticancer drug doxorubicin (DOX) to Panc-1 cancer cells for combination of both chemo- and siRNA-mediated mutant K-Ras gene silencing therapy. Superior anticancer efficacy was observed through synergistic combination of promoted siRNA and DOX release upon irradiating the nanoplex formulation with 665 nm light. Our antitumor study shows that the synergistic effect of AuNRs nanoplex formulation with 665 nm light treatment is able to inhibit...
Source: Theranostics - June 13, 2015 Category: Molecular Biology Authors: Feng Yin, Chengbin Yang, Qianqian Wang, Shuwen Zeng, Rui Hu, Guimiao Lin, Jinglin Tian, Siyi Hu, Rong Feng Lan, Ho Sup Yoon, Fei Lu, Kuan Wang, Ken-Tye Yong Tags: Research Paper Source Type: research
Zwitterionic Poly(carboxybetaine)-based Cationic Liposomes for Effective Delivery of Small Interfering RNA Therapeutics without Accelerated Blood Clearance Phenomenon
For efficient delivery of small interfering RNA (siRNA) to the target diseased site in vivo, it is important to design suitable vehicles to control the blood circulation of siRNA. It has been shown that surface modification of cationic liposome/siRNA complexes (lipoplexes) with polyethylene glycol (PEG) could enhance the circulation time of lipoplexes. However, the first injection of PEGylated lipoplexes in vivo induces accelerated blood clearance and enhances hepatic accumulation of the following injected PEGylated lipoplexes, which is known as the accelerated blood clearance (ABC) phenomenon. Herein, we developed zwitter...
Source: Theranostics - June 13, 2015 Category: Molecular Biology Authors: Yan Li, Ruiyuan Liu, Yuanjie Shi, Zhenzhong Zhang, Xin Zhang Tags: Research Paper Source Type: research
Chitosan/siRNA Nanoparticles Targeting Cyclooxygenase Type 2 Attenuate Unilateral Ureteral Obstruction-induced Kidney Injury in Mice
Cyclooxygenase type 2 (COX-2) plays a predominant role in the progression of kidney injury in obstructive nephropathy. The aim of this study was to test the efficacy of chitosan/small interfering RNA (siRNA) nanoparticles to knockdown COX-2 specifically in macrophages to prevent kidney injury induced by unilateral ureteral obstruction (UUO). Using optical imaging techniques and confocal microscopy, we demonstrated that chitosan/siRNA nanoparticles accumulated in macrophages in the obstructed kidney. Consistent with the imaging data, the obstructed kidney contained a higher amount of siRNA and macrophages. Chitosan-formulat...
Source: Theranostics - January 2, 2015 Category: Molecular Biology Authors: Chuanxu Yang, Line Nilsson, Muhammad Umar Cheema, Yan Wang, Jørgen Frøkiær, Shan Gao, Jørgen Kjems, Rikke Nørregaard Tags: Research Paper Source Type: research
Parthenolide Inhibits Cancer Stem-Like Side Population of Nasopharyngeal Carcinoma Cells via Suppression of the NF-κB/COX-2 Pathway
In this study, cyclooxygenase-2 (COX-2) was found to regulate cancer stem-like side population cells of nasopharyngeal carcinoma cells and enhance cancer stem-like cells' characteristics such as higher colony formation efficiency and overexpression of stemness-associated genes. The regulatory effect of COX-2 on cancer stem-like characteristics may be mediated by ABCG2. COX-2 overexpression by a gain-of-function experiment increased the proportion of side population cells and their cancer stemness properties. The present study also demonstrated that in contrast to the classical chemotherapy drug 5-fluorouracil, which increa...
Source: Theranostics - January 1, 2015 Category: Molecular Biology Authors: Kun Liao, Bin Xia, Qun-Ying Zhuang, Meng-Jun Hou, Yu-Jing Zhang, Bing Luo, Yang Qiu, Yan-Fang Gao, Xiao-Jie Li, Hui-Feng Chen, Wen-Hua Ling, Cheng-Yong He, Yi-Jun Huang, Yu-Chun Lin, Zhong-Ning Lin Tags: Research Paper Source Type: research
Cyclodextrin and Polyethylenimine Functionalized Mesoporous Silica Nanoparticles for Delivery of siRNA Cancer Therapeutics
Effective delivery holds the key to successful in vivo application of therapeutic small interfering RNA (siRNA). In this work, we have developed a universal siRNA carrier consisting of a mesoporous silica nanoparticle (MSNP) functionalized with cyclodextrin-grafted polyethylenimine (CP). CP provides positive charge for loading of siRNA through electrostatic interaction and enables effective endosomal escape of siRNA. Using intravital microscopy we were able to monitor tumor enrichment of CP-MSNP/siRNA particles in live mice bearing orthotopic MDA-MB-231 xenograft tumors. CP-MSNP delivery of siRNA targeting the M2 isoform o...
Source: Theranostics - November 15, 2014 Category: Molecular Biology Authors: Jianliang Shen, Han-Cheon Kim, Hua Su, Feng Wang, Joy Wolfram, Dickson Kirui, Junhua Mai, Chaofeng Mu, Liang-Nian Ji, Zong-Wan Mao, Haifa Shen Tags: Research Paper Source Type: research
