Filtered By:
Source: Theranostics
This page shows you your search results in order of relevance.
Order by Relevance | Date
Total 110 results found since Jan 2013.
Co-delivery of Doxorubicin and Bmi1 siRNA by Folate Receptor Targeted Liposomes Exhibits Enhanced Anti-Tumor Effects in vitro and in vivo
Bmi1 gene overexpression is found in various human tumors and has been shown as a potential target for gene treatment. However, siRNA-based treatments targeting Bmi1 gene have been restricted to limited delivery, low bioavailability and hence relatively reduced efficacy. To overcome these barriers, we developed a folate receptor targeted co-delivery system folate-doxorubicin/Bmi1 siRNA liposome (FA-DOX/siRNA-L). The FA-DOX/siRNA-L was prepared through electrostatic interaction between folate doxorubicin liposome (FA-DOX-L) and Bmi1 siRNA. In vitro and in vivo studies showed that FA-DOX/siRNA-L inhibited tumor growth by com...
Source: Theranostics - November 15, 2014 Category: Molecular Biology Authors: Tan Yang, Bin Li, Shibo Qi, Yong Liu, Yongkang Gai, Peng Ye, Guang Yang, Wendian Zhang, Peng Zhang, Xingxing He, Weijie Li, Zhiping Zhang, Guangya Xiang, Chuanrui Xu Tags: Research Paper Source Type: research
ATP-activated decrosslinking and charge-reversal vectors for siRNA delivery and cancer therapy
Conclusion: These results indicated that the ATP-triggered disassembly and charge reversal strategy provided a new way for developing stimuli-responsive siRNA carriers and showed potential for nucleic acid delivery in the treatment of cancer.
Source: Theranostics - December 7, 2018 Category: Molecular Biology Authors: Zhanwei Zhou, Qingyan Zhang, Minghua Zhang, Huipeng Li, Gang Chen, Chenggen Qian, David Oupicky, Minjie Sun Tags: Research Paper Source Type: research
The pH-Triggered Triblock Nanocarrier Enabled Highly Efficient siRNA Delivery for Cancer Therapy
Small interfering RNA (siRNA) therapies have been hampered by lack of delivery systems in the past decades. Nowadays, a few promising vehicles for siRNA delivery have been developed and it is gradually revealed that enhancing siRNA release from endosomes into cytosol is a very important factor for successful delivery. Here, we designed a novel pH-sensitive nanomicelle, PEG-PTTMA-P(GMA-S-DMA) (PTMS), for siRNA delivery. Owing to rapid hydrolysis in acidic environment, PTMS NPs underwent hydrophobic-to-hydrophilic transition in endosomes that enabled combination of proton sponge effect and raised osmotic pressure in endosome...
Source: Theranostics - September 12, 2017 Category: Molecular Biology Authors: Lili Du, Junhui Zhou, Lingwei Meng, Xiaoxia Wang, Changrong Wang, Yuanyu Huang, Shuquan Zheng, Liandong Deng, Huiqing Cao, Zicai Liang, Anjie Dong, Qiang Cheng Tags: Research Paper Source Type: research
Chitosan/siRNA Nanoparticles Targeting Cyclooxygenase Type 2 Attenuate Unilateral Ureteral Obstruction-induced Kidney Injury in Mice
Cyclooxygenase type 2 (COX-2) plays a predominant role in the progression of kidney injury in obstructive nephropathy. The aim of this study was to test the efficacy of chitosan/small interfering RNA (siRNA) nanoparticles to knockdown COX-2 specifically in macrophages to prevent kidney injury induced by unilateral ureteral obstruction (UUO). Using optical imaging techniques and confocal microscopy, we demonstrated that chitosan/siRNA nanoparticles accumulated in macrophages in the obstructed kidney. Consistent with the imaging data, the obstructed kidney contained a higher amount of siRNA and macrophages. Chitosan-formulat...
Source: Theranostics - January 2, 2015 Category: Molecular Biology Authors: Chuanxu Yang, Line Nilsson, Muhammad Umar Cheema, Yan Wang, Jørgen Frøkiær, Shan Gao, Jørgen Kjems, Rikke Nørregaard Tags: Research Paper Source Type: research
Therapeutic effects of human monoclonal PSMA antibody-mediated TRIM24 siRNA delivery in PSMA-positive castration-resistant prostate cancer
Conclusion: PSMAb mediated TRIM24 siRNA delivery platform could significantly inhibit cell proliferation, colony-formation, and invasion in PSMA+ CRPC in vitro and suppressed tumor growth and bone loss in PSMA+ CRPC xenograft and bone metastasis model.
Source: Theranostics - June 13, 2019 Category: Molecular Biology Authors: Sheng-Jia Shi, Li-Juan Wang, Dong-Hui Han, Jie-Heng Wu, Dian Jiao, Kai-Liang Zhang, Jiang-Wei Chen, Yu Li, Fa Yang, Jing-Liang Zhang, Guo-Xu Zheng, An-Gang Yang, Ai-Zhi Zhao, Wei-Jun Qin, Wei-Hong Wen Tags: Research Paper Source Type: research
Biocompatible AIEgen/p-glycoprotein siRNA@reduction-sensitive paclitaxel polymeric prodrug nanoparticles for overcoming chemotherapy resistance in ovarian cancer
Conclusion: Our findings demonstrated the Py-TPE/siRNA@PMP as a promising agent for the highly efficient treatment of PTX-resistant cells and overcoming the shortage of chemotherapy in ovarian cancer.
Source: Theranostics - April 19, 2021 Category: Molecular Biology Authors: Jun Wu, Quan Wang, Xiaoqi Dong, Min Xu, Juliang Yang, Xiaoqing Yi, Biao Chen, Xiyuan Dong, Ying Wang, Xiaoding Lou, Fan Xia, Shixuan Wang, Jun Dai Tags: Research Paper Source Type: research
High-Yield Synthesis of Monomeric LMWP(CPP)-siRNA Covalent Conjugate for Effective Cytosolic Delivery of siRNA
Because of the unparalleled efficiency and universal utility in treating a variety of disease types, siRNA agents have evolved as the future drug-of-choice. Yet, the inability of the polyanionic siRNA macromolecules to cross the cell membrane remains as the bottleneck of possible clinical applications. With the cell penetrating peptides (CPP) being discovered lately, the most effective tactic to achieve the highest intracellular siRNA delivery deems to be by covalently conjugating the drug to a CPP; for instance, the arginine-rich Tat or low molecular weight protamine (LMWP) peptides. However, construction of such a chemic...
Source: Theranostics - September 12, 2017 Category: Molecular Biology Authors: Junxiao Ye, Ergang Liu, Junbo Gong, Jianxin Wang, Yongzhuo Huang, Huining He, Victor C. Yang Tags: Research Paper Source Type: research
Mono-arginine Cholesterol-based Small Lipid Nanoparticles as a Systemic siRNA Delivery Platform for Effective Cancer Therapy
This study describes the development of neutral, small lipid nanoparticles (SLNPs) made of a nontoxic cationic cholesterol derivative, as a suitable carrier of systemic siRNA to treat cancers. The cationic cholesterol derivative, mono arginine-cholesterol (MA-Chol), was synthesized by directly attaching an arginine moiety to cholesterol via a cleavable ester bond. siRNA-loaded SLNPs (siRNA@SLNPs) were prepared using MA-Chol and a neutral helper lipid, dioleoyl phosphatidylethanolamine (DOPE), as major components and a small amount of PEGylated phospholipid mixed with siRNA. The resulting nanoparticles were less than ~...
Source: Theranostics - June 5, 2016 Category: Molecular Biology Authors: Jinju Lee, Phei Er Saw, Vipul Gujrati, Yonghyun Lee, Hyungjun Kim, Sukmo Kang, Minsuk Choi, Jae-Il Kim, Sangyong Jon Tags: Research Paper Source Type: research
Pharmacokinetic Behaviors of Intravenously Administered siRNA in Glandular Tissues
In this study, we comprehensively investigated the behavior of siRNAs in vivo in various tissues and demonstrated that intravenously-injected naked siRNA accumulated remarkably in the submandibular gland, bulbourethral gland, and pancreas, with a respective half-life of ~22.7, ~45.6, and ~30.3 h. This was further confirmed by gel separation of tissue homogenates and/or supernatants. In vivo imaging and cryosectioning suggested that delivery carriers significantly influence the distribution and elimination profiles of siRNA. Gene-silencing assays revealed that neither naked nor liposome-formulated siRNA resul...
Source: Theranostics - June 26, 2016 Category: Molecular Biology Authors: Yuanyu Huang, Qiang Cheng, Jia-Li Ji, Shuquan Zheng, Lili Du, Lingwei Meng, Yidi Wu, Deyao Zhao, Xiaoxia Wang, Li Lai, Huiqing Cao, Kai Xiao, Shan Gao, Zicai Liang Tags: Research Paper Source Type: research
Cyclodextrin and Polyethylenimine Functionalized Mesoporous Silica Nanoparticles for Delivery of siRNA Cancer Therapeutics
Effective delivery holds the key to successful in vivo application of therapeutic small interfering RNA (siRNA). In this work, we have developed a universal siRNA carrier consisting of a mesoporous silica nanoparticle (MSNP) functionalized with cyclodextrin-grafted polyethylenimine (CP). CP provides positive charge for loading of siRNA through electrostatic interaction and enables effective endosomal escape of siRNA. Using intravital microscopy we were able to monitor tumor enrichment of CP-MSNP/siRNA particles in live mice bearing orthotopic MDA-MB-231 xenograft tumors. CP-MSNP delivery of siRNA targeting the M2 isoform o...
Source: Theranostics - November 15, 2014 Category: Molecular Biology Authors: Jianliang Shen, Han-Cheon Kim, Hua Su, Feng Wang, Joy Wolfram, Dickson Kirui, Junhua Mai, Chaofeng Mu, Liang-Nian Ji, Zong-Wan Mao, Haifa Shen Tags: Research Paper Source Type: research
Acid-degradable Dextran as an Image Guided siRNA Carrier for COX-2 Downregulation
Conclusions: We successfully developed an efficient method to produce an acid-degradable dextran nanopolymer containing cleavable amine groups as the siRNA carrier. Because of its biocompatibility, this degradable dextran delivered COX-2 siRNA within tumors and efficiently downregulated COX-2 expression.
Source: Theranostics - June 20, 2018 Category: Molecular Biology Authors: Zhihang Chen, Balaji Krishnamachary, Marie-France Penet, Zaver M. Bhujwalla Tags: Research Paper Source Type: research
Development of a Biocompatible Copolymer Nanocomplex to Deliver VEGF siRNA for Triple Negative Breast Cancer
Conclusion: PLEGP1800 is a safe and efficient copolymer to deliver siRNAs for TNBC therapy. It could potentially be applied to other cancers by changing the cargo and incorporating tumor-specific ligands.
Source: Theranostics - June 13, 2019 Category: Molecular Biology Authors: Zhen Zhao, Yuanke Li, Ravi Shukla, Hao Liu, Akshay Jain, Ashutosh Barve, Kun Cheng Tags: Research Paper Source Type: research
Thyroid hormone plus dual-specificity phosphatase-5 siRNA increases the number of cardiac muscle cells and improves left ventricular contractile function in chronic doxorubicin-injured hearts
Conclusion: RNA therapies are showing great potential. Importantly, a GMP compliant in vivo-jetPEI system for delivery of siRNA is already in use in humans, as is T3. Given these considerations, our findings provide a potentially highly translatable strategy for addressing doxorubicin cardiomyopathy, a currently untreatable condition.
Source: Theranostics - April 19, 2021 Category: Molecular Biology Authors: Lin Tan, Nikolay Bogush, Emmen Naqvi, John W. Calvert, Robert M. Graham, W. Robert Taylor, Nawazish Naqvi, Ahsan Husain Tags: Research Paper Source Type: research
Megalin-Mediated Specific Uptake of Chitosan/siRNA Nanoparticles in Mouse Kidney Proximal Tubule Epithelial Cells Enables AQP1 Gene Silencing
In conclusion, specific targeting PTECs with the chitosan nanoparticle system may prove to be a useful strategy for knockdown of specific genes in PTECs, and provides a potential therapeutic strategy for treating various kidney diseases.
Source: Theranostics - November 15, 2014 Category: Molecular Biology Authors: Shan Gao, San Hein, Frederik Dagnæs-Hansen, Kathrin Weyer, Chuanxu Yang, Rikke Nielsen, Erik I Christensen, Robert A Fenton, Jørgen Kjems Tags: Research Paper Source Type: research
Triple-Layered pH-Responsive Micelleplexes Loaded with siRNA and Cisplatin Prodrug for NF-Kappa B Targeted Treatment of Metastatic Breast Cancer
The combination of chemotherapy and RNA interference is a promising approach for efficient cancer therapy. However, the success of such a strategy is hampered by the lack of suitable vectors to coordinate small interfering RNA (siRNA) and chemotherapeutic drug into one single platform. We herein report a novel triple-layered pH-responsive micelleplex loading siRNA and alkylated cisplatin prodrug for NF-Kappa B targeted treatment of metastatic breast cancer. The micelles were self-assembled from poly(ethylene glycol)-block-poly(aminolated glycidyl methacrylate)-block-poly(2-(diisopropyl amino) ethyl methacrylate) (PEG-b-PAG...
Source: Theranostics - June 5, 2016 Category: Molecular Biology Authors: Haijun Yu, Chengyue Guo, Bing Feng, Jianping Liu, Xianzhi Chen, Dangge Wang, Lesheng Teng, Youxin Li, Qi Yin, Zhiwen Zhang, Yaping Li Tags: Research Paper Source Type: research
