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Source: Nanomedicine: Nanotechnology, Biology and Medicine
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Total 65 results found since Jan 2013.
Intracellular Trafficking and Exocytosis of a Multi-Component siRNA Nanocomplex
The objective of this study is to study post internalization trafficking of this siRNA nanocomplex and its multiple components like siRNA, protamine, and streptavidin, in HSCs. After internalization, the nanocomplex entrapped in early endosomes undergoes three possible routes including endosomal escape, exocytosis, and entrapment in lysosomes. Significant amount of siRNA dissociates form the nanocomplex to exert silencing activity. After escaping from endosomes, protamine dissociates from the nanocomplex and stays inside the cytoplasm. Golgi complex plays an important role in exocytosis of the nanocomplex. We also demonstr...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 11, 2016 Category: Nanotechnology Source Type: research
Delivery of siRNA using folate receptor-targeted pH-sensitive polymeric nanoparticles for rheumatoid arthritis therapy
Publication date: Available online 22 May 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Xiangshi Sun, Shiyan Dong, Xiangyu Li, Kongtong Yu, Fengying Sun, Robert J. Lee, Youxin Li, Lesheng TengAbstractSystemic delivery of siRNA to target tissues is difficult to achieve owing to its limited cellular uptake and poor serum stability. Herein, polymeric nanoparticles were developed for systemic administration of siRNA to inflamed tissues. The polymeric nanoparticles were composed of PK3 as a pH-sensitive polymer, folate-polyethyleneglycol-poly(lactide-co-glycolide) as a targeting ligand, and a DOTAP/si...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - May 23, 2019 Category: Nanotechnology Source Type: research
Conformation-dependent binding and tumor-targeted delivery of siRNA by a designed TRBP2: Affibody fusion protein
Publication date: Available online 23 February 2015 Source:Nanomedicine: Nanotechnology, Biology and Medicine Author(s): Ghulam Hassan Dar , Vijaya Gopal , Madhusudhana Rao Efficiency of systemically delivered siRNA in gene silencing is compromised due to lack of target-specific delivery and rapid clearance of siRNA by in vivo elimination pathways. We designed a fusion protein consisting of a dsRNA binding domain of Transactivation Response RNA Binding Protein (TRBP2) fused to ErbB2 binding affibody (AF) for target specific delivery of siRNA. Designated as TRAF, the fusion protein is stable and binds efficiently and spec...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - February 24, 2015 Category: Nanotechnology Source Type: research
Single-Cell Kinetics of siRNA-Mediated mRNA Degradation
Publication date: Available online 8 August 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Rafał Krzysztoń, Daniel Woschée, Anita Reiser, Gerlinde Schwake, Helmut H. Strey, Joachim O. RädlerAbstractRNA interference (RNAi) enables the therapeutic use of small interfering RNAs (siRNAs) to silence disease-related genes. The efficiency of silencing is commonly assessed by measuring expression levels of the target protein at a given time point post-transfection. Here, we determine the siRNA-induced fold change in mRNA degradation kinetics from single-cell fluorescence time-courses obtained using li...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - August 8, 2019 Category: Nanotechnology Source Type: research
Designing idiosyncratic hmPCL-siRNA Nanoformulated Capsules for Silencing and Cancer Therapy
Publication date: Available online 10 November 2015 Source:Nanomedicine: Nanotechnology, Biology and Medicine Author(s): Vijay Bhooshon Kumar, Himadri Medhi, Zhang Yong, Pradip Paik In this work, we have designed a siRNA-nanoformulation with mesoporous polycaprolactone (hmPCL) for silencing and cancer therapy. Average hollow core size of hmPCL nanocapsules used for nanoformulation is ~180 nm with shell thickness of 10-20 nm and mesopore size of ~5-10 nm in diameter. Idiosyncratic capsules are biocompatible which has been confirmed with normal lymphocyte, K562 leukaemia cancer cells and on HepG2/EGFP cancer cells. In ...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - November 10, 2015 Category: Nanotechnology Source Type: research
Ultrasound-Responsive Microbubbles for Sonography-guided siRNA Delivery
Publication date: Available online 28 December 2015 Source:Nanomedicine: Nanotechnology, Biology and Medicine Author(s): Ping Wang, Tinghui Yin, Jingguo Li, Bowen Zheng, Xiaoli Wang, Yiru Wang, Jian Zheng, Rongqin Zheng, Xintao Shuai RNA interfering is a gene therapeutic approach of great potential for cancer. However, tumor-targeted delivery of small interfering RNA (siRNA) solely based on the enhanced permeability and retention effect of nanocarriers is often insufficient. To address this challenge, siRNA encapsulated ultrasound-responsive microbubble (MB) was developed from polymeric siRNA micelles and liposo...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - December 29, 2015 Category: Nanotechnology Source Type: research
Mannose-conjugated layered double hydroxide nanocomposite for targeted siRNA delivery to enhance cancer therapy
Publication date: Available online 22 June 2017Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Li Li, Run Zhang, Wenyi Gu, Zhi Ping XuAbstractSheet-like layered double hydroxide nanoparticles (LDH NPs) have showed great potentials in biomedical applications such as nanocarriers for drug and gene delivery, biosensors and imaging agents. However, target delivery of drugs and genes using LDH NPs to the desired tumor sites is a major challenge in cancer therapy. The aim of this study is to develop a functional LDH-based nanocomposite for target delivery of siRNA to cancer cells. Mannose as a targeting moie...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - July 5, 2018 Category: Nanotechnology Source Type: research
Rational modification of oligoarginine for highly efficient siRNA delivery: structure–activity relationship and mechanism of intracellular trafficking of siRNA
This study present a novel cell penetrating peptide-based siRNA delivery system utilizing modified oligo-arginine demonstrating a successful siRNA delivery approach. Graphical abstract
Source: Nanomedicine: Nanotechnology, Biology and Medicine - February 13, 2015 Category: Nanotechnology Source Type: research
Intranasal Brain Delivery of Cationic Nanoemulsion-Encapsulated TNFα siRNA in Prevention of Experimental Neuroinflammation
Publication date: Available online 6 January 2016 Source:Nanomedicine: Nanotechnology, Biology and Medicine Author(s): Sunita Yadav, Srujan K. Gandham, Riccardo Panicucci, Mansoor M. Amiji Neuroinflammation is a hallmark of acute and chronic neurodegenerative disorders. Activated microglia and secreted factors such as tumor necrosis factor-alpha (TNFα) are key mediators of neuroinflammation and may contribute to neuronal dysfunction. The main aim of this study was to evaluate the therapeutic efficacy of intranasal cationic nanoemulsions encapsulating an anti-TNFα siRNA, for potential anti-inflammatory therapy, test...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - January 12, 2016 Category: Nanotechnology Source Type: research
Enriched chitosan nanoparticles loaded with siRNA are effective in lowering Huntington's disease gene expression following intranasal administration
Publication date: Available online 27 October 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Vasyl Sava, Oksana Fihurka, Anastasia Khvorova, Juan Sanchez-RamosAbstractTherapies to lower gene expression in brain disease currently require chronic administration into the cerebrospinal fluid (CSF) by intrathecal infusions or direct intracerebral injections. Though well-tolerated in the short-term, this approach is not tenable for a life-time of administration. Nose-to-brain delivery of enriched chitosan-based nanoparticles loaded with anti-HTT siRNA was studied in a transgenic YAC128 mouse model of Hu...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - October 28, 2019 Category: Nanotechnology Source Type: research
Multifunctional Selenium Nanoparticles: Chiral Selectivity of Delivering MDR-siRNA for Reversal of Multidrug Resistance and Real-time Biofluorescence Imaging
Publication date: Available online 8 May 2015 Source:Nanomedicine: Nanotechnology, Biology and Medicine Author(s): Qingchang Chen , Qianqian Yu , Yanan Liu , Dhairya Bhavsar , Licong Yang , Xiaofan Ren , Dongdong Sun , Wenjing Zheng , Jie Liu , Lan-mei Chen In this paper, chiral selenium nanoparticles (L-SeNPs/D-SeNPs) modified with a dinuclear Ruthenium (II) complex were used to effectively deliver siRNA targeting the MDR1 gene. In this co-delivery system, the luminescent dinuclear Ruthenium (II) complex was developed to act as a gene carrier and anti-tumor drug, while offering luminescent imaging to follow the intracel...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - May 9, 2015 Category: Nanotechnology Source Type: research
Robust neuroprotective effects of intranasally delivered iNOS siRNA encapsulated in gelatin nanoparticles in the postischemic brain
Publication date: Available online 3 March 2016 Source:Nanomedicine: Nanotechnology, Biology and Medicine Author(s): Il-Doo Kim, Elizabeth Sawicki, Hye-Kyung Lee, Eun-Hwa Lee, Heon Joo Park, Pyung-Lim Han, Kyekyoon (Kevin) Kim, Hyungsoo Choi, Ja-Kyeong Lee The therapeutic efficacy of intranasal iNOS siRNA delivery was investigated in the postischemic rat brain after encapsulating on in gelatin nanoparticles (GNPs; diameter 188.0±60.9 nm) cross-linked with 0.0667% glutaraldehyde (GA). Intranasally delivered GNPs were found in extracellular and intracellular compartments of many brain regions, including the olfac...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 4, 2016 Category: Nanotechnology Source Type: research
Mannose-conjugated layered double hydroxide nanocomposite for targeted siRNA delivery to enhanced cancer therapy
Publication date: Available online 22 June 2017 Source:Nanomedicine: Nanotechnology, Biology and Medicine Author(s): Li Li, Run Zhang, Wenyi Gu, Zhi Ping Xu Sheet-like layered double hydroxide nanoparticles (LDH NPs) have showed great potentials in biomedical applications such as nanocarriers for drug and gene delivery, biosensors and imaging agents. However, target delivery of drugs and genes using LDH NPs to the desired tumor sites is a major challenge in cancer therapy. The aim of this study is to develop a functional LDH-based nanocomposite for target delivery of siRNA to cancer cells. Mannose as a targeting moiety wa...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - June 23, 2017 Category: Nanotechnology Source Type: research
Mannose-conjugated layered double hydroxide nanocomposite for targeted siRNA delivery to enhance cancer therapy
Publication date: Available online 22 June 2017 Source:Nanomedicine: Nanotechnology, Biology and Medicine Author(s): Li Li, Run Zhang, Wenyi Gu, Zhi Ping Xu Sheet-like layered double hydroxide nanoparticles (LDH NPs) have showed great potentials in biomedical applications such as nanocarriers for drug and gene delivery, biosensors and imaging agents. However, target delivery of drugs and genes using LDH NPs to the desired tumor sites is a major challenge in cancer therapy. The aim of this study is to develop a functional LDH-based nanocomposite for target delivery of siRNA to cancer cells. Mannose as a targeting moiety wa...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - July 1, 2017 Category: Nanotechnology Source Type: research
