Mannose-conjugated layered double hydroxide nanocomposite for targeted siRNA delivery to enhance cancer therapy

Publication date: Available online 22 June 2017Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Li Li, Run Zhang, Wenyi Gu, Zhi Ping XuAbstractSheet-like layered double hydroxide nanoparticles (LDH NPs) have showed great potentials in biomedical applications such as nanocarriers for drug and gene delivery, biosensors and imaging agents. However, target delivery of drugs and genes using LDH NPs to the desired tumor sites is a major challenge in cancer therapy. The aim of this study is to develop a functional LDH-based nanocomposite for target delivery of siRNA to cancer cells. Mannose as a targeting moiety was firstly conjugated onto SiO2-coated LDH nanocomposite. Cellular uptake data have demonstrated that siRNA is more efficiently delivered to osteosarcoma (U2OS) cells by mannose-conjugated SiO2 coated LDH nanocomposite (Man-SiO2@LDH) compared to unmodified LDH NP. A commercial cell death-siRNA (CD-siRNA) delivered by Man-SiO2@LDH can kill cancer cells more effectively. These results reveal that the Man-SiO2@LDH nanocomposite is capable of target-delivering siRNA or drugs to tumor cells for more effective cancer treatment, which provides great potentials in cancer therapy.Graphical AbstractIn this work, mannose-conjugated SiO2-coated LDH (Man-SiO2@LDH) nanocomposite has been developed by grafting mannose onto nNH2-SiO2@LDH. This sheet-like Man-SiO2@LDH nanocomposite has resulted in more efficient uptake of siRNA by U2OS cells via receptor-mediated interna...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - Category: Nanotechnology Source Type: research