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TLR4 (Toll-Like Receptor 4)-Dependent Signaling Drives Extracellular Catabolism of LDL (Low-Density Lipoprotein) Aggregates.
CONCLUSIONS: We present a mechanism in which interaction of macrophages with agLDL initiates a TLR4 signaling pathway, resulting in formation of the lysosomal synapse, catabolism of agLDL, and lipid accumulation in vitro and in vivo. PMID: 31597445 [PubMed - as supplied by publisher]
Source: Arteriosclerosis, Thrombosis and Vascular Biology - October 9, 2019 Category: Cardiology Authors: Singh RK, Haka AS, Asmal A, Barbosa-Lorenzi VC, Grosheva I, Chin HF, Xiong Y, Hla T, Maxfield FR Tags: Arterioscler Thromb Vasc Biol Source Type: research

A Critical Role of Growth Arrest-Specific Gene 6-Mer Axis in the Pathogenesis of Endothelial Damage Contributing to Thrombotic Microangiopathy Associated with Graft-Versus-Host Disease after Allogeneic Hematopoietic Stem Cell Transplantation
Graft-versus-host disease (GVHD) and thrombotic microangiopathy (TMA) are severe complications of allogeneic hematopoietic stem cell transplantation (allo-SCT). Host endothelial cells are targets of alloreactive donor cytotoxic T lymphocytes or various proinflammatory cytokines following allo-SCT, and endothelial damage plays an important role in the pathogenic mechanism(s) of TMA associated with GVHD. However, the detailed mechanism(s) of TMA as well as GVHD have not yet been fully elucidated. Growth arrest-specific (Gas) 6 structurally belongs to the family of plasma vitamin K-dependent proteins working as a cofactor for...
Source: Blood - November 21, 2018 Category: Hematology Authors: Furukawa, M., Xintao, W., Ohkawara, H., Fukatsu, M., Alkebsi, L., Noji, H., Ogawa, K., Ikezoe, T. Tags: 701. Experimental Transplantation: Basic Biology, Pre-Clinical Models: Poster III Source Type: research

siRNA Mediated Knockdown of Tissue Factor Expression in Pigs for Xenotransplantation
Abstract Acute vascular rejection (AVR), in particular microvascular thrombosis, is an important barrier to successful pig‐to‐primate xenotransplantation. Here, we report the generation of pigs with decreased tissue factor (TF) levels induced by small interfering (si)RNA‐mediated gene silencing. Porcine fibroblasts were transfected with TF‐targeting small hairpin (sh)RNA and used for somatic cell nuclear transfer. Offspring were analyzed for siRNA, TF mRNA and TF protein level. Functionality of TF downregulation was investigated by a whole blood clotting test and a flow chamber assay. TF siRNA was expressed in all ...
Source: American Journal of Transplantation - March 23, 2015 Category: Transplant Surgery Authors: H. E. Ahrens, B. Petersen, D. Herrmann, A. Lucas‐Hahn, P. Hassel, M. Ziegler, W. A. Kues, U. Baulain, W. Baars, R. Schwinzer, J. Denner, D. Rataj, S. Werwitzke, A. Tiede, A. K. Bongoni, P. S. Garimella, A. Despont, R. Rieben, H. Niemann Tags: Brief Communication Source Type: research

Interferon-Induced Transmembrane Protein 1 Regulates Endothelial Lumen Formation During Angiogenesis.
CONCLUSIONS: IFITM1 is essential for the formation of functional blood vessels and stabilizes EC-EC interactions during endothelial lumen formation by regulating tight junction assembly. PMID: 24603679 [PubMed - as supplied by publisher]
Source: Arteriosclerosis, Thrombosis and Vascular Biology - March 6, 2014 Category: Cardiology Authors: Popson SA, Ziegler ME, Chen X, Holderfield MT, Shaaban CI, Fong AH, Welch-Reardon KM, Papkoff J, Hughes CC Tags: Arterioscler Thromb Vasc Biol Source Type: research

Hypoxia Inhibits Cellular Senescence to Restore the Therapeutic Potential of Old Human Endothelial Progenitor Cells via the Hypoxia-Inducible Factor-1α-TWIST-p21 Axis.
CONCLUSIONS: This study introduces ex vivo expansion protocols involving hypoxic preconditioning that are suitable for efficiently expanding old EPCs without senescence through modulation of the hypoxia-induced hypoxia-inducible factor-1α-TWIST-p21 axis. In addition, the expanded cells are shown to be useful for therapeutic vasculogenesis. PMID: 23928864 [PubMed - as supplied by publisher]
Source: Arteriosclerosis, Thrombosis and Vascular Biology - August 8, 2013 Category: Cardiology Authors: Lee SH, Lee JH, Yoo SY, Hur J, Kim HS, Kwon SM Tags: Arterioscler Thromb Vasc Biol Source Type: research

Zink finger nucleases and siRNAs: use in transgenic pig production for xenotransplantation
Emerging technologies, including the use of specific nucleases, transposons and specific siRNAs will bring significant improvements with regard to efficiency, precision and safety of gene transfer. Here, we summarize recent findings from our laboratory on the integration of Zinc‐finger nuclease technology (ZFNs) and specific siRNAs into a research programme towards the development and characterization of multi‐transgenic pigs suitable for xenotransplantation. Zinc‐finger nucleases (ZFNs) are powerful tools for producing gene knockouts (KOs) with very high efficiency. ZFNs were used to induce a biallelic knockout of t...
Source: Xenotransplantation - February 5, 2013 Category: Transplant Surgery Authors: Heiner Niemann, Björn Petersen, Hellen Ahrens, Wilfried Kues Source Type: research