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Notch/NICD/RBP-J signaling axis regulates M1 polarization of macrophages mediated by advanced glycation end products
AbstractAdvanced glycation end products (AGEs) aggregation and macrophages polarization are identified as the main factors contributing to bone diseases caused by aging or diabetes, such as senile or diabetic osteoporosis. Here, we aimed to elucidate the involvement and potential mechanism of AGEs in macrophages polarization and osteoclastogenesis. Firstly, AGEs-treated RAW264.7 macrophages were observed to up-regulate the release of nitric oxide (NO), the expression of M1-associated genes and the surface antigen marker CD86. The detection of osteoclast-related markers and TRAP staining revealed that the osteoclastogenic a...
Source: Glycoconjugate Journal - June 6, 2022 Category: Biochemistry Source Type: research

RNA interference-based osteoanabolic therapy for osteoporosis by a bone-formation surface targeting delivery system
In this study, we identified casein kinase-2 interacting protein-1 encoding gene (Ckip-1), a negative regulator of bone formation, as an effective target of small interfering RNAs (siRNAs) for improving bone mass. Moreover, an impressive (DSS)6-Liposome (Lipos) nanoparticle system that could target the bone formation surface was synthesized to enhance the delivery of Ckip-1 siRNA to osteogenic lineage cells. The in vitro results confirmed that the (DSS)6-Lipos system could efficaciously improve the intracellular delivery of Ckip-1 siRNA without obvious cell toxicity. The in vivo application of the delivery system showed sp...
Source: Biochemical and Biophysical Research communications - March 1, 2022 Category: Biochemistry Authors: Ye Gao He Xin Bolei Cai Le Wang Qianxin Lv Yan Hou Fuwei Liu Taiqiang Dai Liang Kong Source Type: research

Resveratrol induces proliferation in preosteoblast cell MC3T3-E1 via GATA-1 activating autophagy
We examined the cell viability, colony formation, cell cyclin expression, autophagy level, and the expression levels of GATA-1 and adenosine 5'-monophosphate (AMP)-activated protein kinase α (AMPKα) in osteoblastic cell strain MC3T3-E1. The results showed that RSV promoted the proliferation process in MC3T3-E1 coupled with increased expression of GATA-1 and phosphorylated AMPKα and activated autophagy. When GATA-1 was interfered with siRNA, both autophagy and proliferation were decreased. Administration of the agonist of phosphorylated AMPKα1 (Thr172) promoted the translocation of GATA-1 into the nucleus. Based on the ...
Source: Acta Biochimica et Biophysica Sinica - October 12, 2021 Category: Biochemistry Authors: Xiang Liu Jun Tao Yueyi Yao Ping Yang Juhui Wang Mali Yu Jianhong Hou Ying Zhang L I Gui Source Type: research

Propionate promotes vitamin D receptor expression via yes-associated protein in rats with short bowel syndrome.
In this study, we aimed to investigate the effect of propionate on the expression of the vitamin D receptor (VDR) in the small intestine of rats with SBS. Firstly, IEC-6 (intestinal epithelioid cell line No. 6) cells were incubated in vitro with 1 mM sodium propionate for 24 h. This resulted in a significant increase in the expression of VDR and yes-associated protein (YAP) compared with that in the control group. Transfection of IEC-6 cells with YAP siRNA significantly down-regulated the expression of VDR. By contrast, after incubating IEC-6 cells with lysophosphatidic acid, an agonist of YAP, upregulation of VDR and...
Source: Biochemical and Biophysical Research communications - January 11, 2020 Category: Biochemistry Authors: Lin H, Huang Y, Tian T, Wang P, Li Y Tags: Biochem Biophys Res Commun Source Type: research

Mangiferin protects osteoblast against oxidative damage by modulation of ERK5/Nrf2 signaling.
This study indicated that MAN can protect osteoblast against oxidative damage by modulation of ERK5/Nrf2 signaling, which can be new agent for osteoporosis. PMID: 28669729 [PubMed - as supplied by publisher]
Source: Biochemical and Biophysical Research communications - June 29, 2017 Category: Biochemistry Authors: Xia G, Li X, Yin X, Ding H, Qiao Y Tags: Biochem Biophys Res Commun Source Type: research

FGF Suppresses Poldip2 Expression in Osteoblasts
This article is protected by copyright. All rights reserved
Source: Journal of Cellular Biochemistry - December 4, 2016 Category: Biochemistry Authors: Sakie Katsumura, Yayoi Izu, Takayuki Yamada, Kathy Griendling, Kiyoshi Harada, Masaki Noda, Yoichi Ezura Tags: Article Source Type: research

MEK5 suppresses osteoblastic differentiation.
In this study, we have demonstrated the role of MEK5 in osteoblastic differentiation in mouse preosteoblastic MC3T3-E1 cells and bone marrow stromal ST2 cells. We found that treatment with BIX02189, an inhibitor of MEK5, increased alkaline phosphatase (ALP) activity and the gene expression of ALP, osteocalcin (OCN) and osterix, as well as it enhanced the calcification of the extracellular matrix. Moreover, osteoblastic cell proliferation decreased at a concentration of greater than 0.5 μM. In addition, knockdown of MEK5 using siRNA induced an increase in ALP activity and in the gene expression of ALP, OCN, and osterix. In...
Source: Biochemical and Biophysical Research communications - May 18, 2015 Category: Biochemistry Authors: Kaneshiro S, Otsuki D, Yoshida K, Yoshikawa H, Higuchi C Tags: Biochem Biophys Res Commun Source Type: research

MiR-21 overexpression improves osteoporosis by targeting RECK.
Abstract Osteoporosis is a kind of metabolic bone disorder. MicroRNA-21 (miR-21) has been proven to play an important role in bone formation, whereas its role in osteoporosis is unclear. In the present study, miR-21 expression was inhibited by TNF-α in mesenchymal stem cells (MSCs). TNF-α induced cell apoptosis, and inhibited cell proliferation and differentiation of MSCs. Whereas the effect was reversed by miR-21 mimics. Expression of reversion-inducing cysteine-rich protein with Kazal motifs (RECK) which is a predicted target of miR-21 was inhibited by miR-21 mimics. A luciferase reporter gene assay showed th...
Source: Molecular and Cellular Biochemistry - April 17, 2015 Category: Biochemistry Authors: Zhao W, Dong Y, Wu C, Ma Y, Jin Y, Ji Y Tags: Mol Cell Biochem Source Type: research

Indole-3-carbinol as inhibitors of glucocorticoid-induced apoptosis in osteoblastic cells through blocking ROS-mediated Nrf2 pathway.
In this study, we showed that, indole-3-carbinol (I3C), a natural product found in broadly consumed plants of the Brassica genus, could block the cytotoxic effects of dexamethasone (Dex), and elucidated the underlying molecular mechanisms. Firstly, we showed that, I3C could effectively suppress Dex-induced cytotoxicity and apoptotic cell death in osteoblastic cells, as evidenced by the decrease in Sub-G1 cell population. Treatment of the cells with Dex resulted in activation of caspase-3/-8/-9 and subsequent cleavage of PARP, which was also effectively blocked by co-incubation of I3C. Moreover, exposure to Dex triggered a ...
Source: Biochemical and Biophysical Research communications - March 17, 2015 Category: Biochemistry Authors: Lin H, Gao X, Chen G, Sun J, Chu J, Jing K, Li P, Zeng R, Wei B Tags: Biochem Biophys Res Commun Source Type: research

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