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SIRT1 exerts anti-hypertensive effect via FOXO1 activation in the rostral ventrolateral medulla
Free Radic Biol Med. 2022 Jun 7:S0891-5849(22)00223-4. doi: 10.1016/j.freeradbiomed.2022.06.003. Online ahead of print.ABSTRACTThe rostral ventrolateral medulla (RVLM) is a pivotal region in the central regulation of blood pressure (BP). It has been documented that silent information regulator 2 homolog 1 (SIRT1), a nicotinamide adenine dinucleotide (NAD+)-dependent multifunctional transcription regulatory factor, has many cardiovascular protective effects. However, the role and significance of SIRT1 in the central regulation of cardiovascular activity, especially in RVLM, remains unknown. Therefore, the aim of this study ...
Source: Free Radical Biology and Medicine - June 10, 2022 Category: Biology Authors: Chang-Zhen Ren Zhao-Tang Wu Wen Wang Xing Tan Ya-Hong Yang Yang-Kai Wang Miao-Ling Li Wei-Zhong Wang Source Type: research

Protective Effect of Mitogen- and Stress-Activated Protein Kinase on the Rats with Focal Ischemia-Reperfusion Injury
In conclusion, MSK exerted a protective effect on rat with focal ischemia-reperfusion injury through its anti-apoptotic effect on neurons and anti-inflammatory effect on astrocytes.
Source: Inflammation - November 14, 2019 Category: Allergy & Immunology Source Type: research

Oxidant-induced increase in norepinephrine secretion from PC12  cells is dependent on TRPM8 channel-mediated intracellular calcium elevation.
In this study, we show that cold-sensitive receptor TRPM8 is activated by pro-oxidant tert-butyl hydroperoxide (tBHP). Polymerase chain reaction, Western immunoblotting, and immunofluorescence indicated that TRPM8 channels are expressed in rat pheochromocytoma 12 (PC12) cells, a phenotypic model of sympathetic neurosecretion when differentiated with nerve growth factor. WS-12, a selective TRPM8 channel agonist, and tBHP increased intracellular Ca2+ concentration in differentiated PC12 cells; an effect attenuated by AMTB, a selective TRPM8 channel blocker, and siRNA-mediated TRPM8 knockdown. Blockade of TRPM8 channels als...
Source: Biochemical and Biophysical Research communications - October 27, 2018 Category: Biochemistry Authors: Peixoto-Neves D, Soni H, Adebiyi A Tags: Biochem Biophys Res Commun Source Type: research

The deubiquitinating enzyme UBPy/USP8 interacts with TrkA and inhibits neuronal differentiation in PC12 cells.
In this study we demonstrated that in PC12 cells the TrkA receptor interacts with the deubiquitinating enzyme USP8/UBPy in a NGF-dependent manner, and that it is deubiquitinated in vivo and in vitro by USP8. USP8 overexpression blocked NGF-induced neurites outgrowth while the overexpression of the catalytically inactive mutant USP8/UBPy(C748A) caused a marked increase of cell differentiation. Localization and biochemical experiments have point out that USP8 and TrkA partially co-localize in endosomes after NGF stimulation. Finally we have studied the role played by USP8 on TrkA turnover; using specific siRNA for USP8 we fo...
Source: Experimental Cell Research - February 4, 2015 Category: Cytology Authors: Ceriani M, Amigoni L, D'Aloia A, Berruti G, Martegani E Tags: Exp Cell Res Source Type: research