• Filter By:
• Specialty
• Source
• Condition
• Cancer
• Infectious Disease
• Drug
• Training
• Management
• Procedure
• Therapy
• Vaccine
• Nutrition
• Country

GSE86179 Knockdowns of total NFYA and a NFYA long 3 ’ UTR isoform followed by RNA-seq to determine isoform-specific effects on gene expression
Contributors : Thomas J Sweet ; Jeffrey M Bhasin ; Angela H TingSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensAlternative cleavage and polyadenylation (APA) produces multiple isoforms of the Nuclear Transcription Factor Y Subunit Alpha (NFYA) gene with variable length 3? untranslated regions (UTRs). These isoforms have been detected in HCT116 colon cancer cells via polyA-seq and northern blots, as well as colon cancer tissue RNA-seq data. NFYA is a transcription factor that may regulate cell growth and proliferation in cancer and development. To determine if there may be transcripti...
Source: GEO: Gene Expression Omnibus - December 28, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE191323 Characterization of normal fibroblast transcriptomes upon transient silencing of LTBP2 by RNAseq
Conclusions: Our study explored LTBP2 functions in normal fibroblasts. RNAseq data support its important role. After 48h, LTBP2 silencing induces changes in gene expression of numerous genes involved in fibroblast functions.
Source: GEO: Gene Expression Omnibus - November 22, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE208785 Differentially expressed genes after siRNA transfection in human cancer cell line
Contributors : R Yasudome ; N SekiSeries Type : Expression profiling by arrayOrganism : Homo sapiensTo identify differentially expressed genes by siRNAs transfection in human colon cancer cell line, HCT116 was subjected to Agilent whole genome microarrays.
Source: GEO: Gene Expression Omnibus - July 31, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

GSE164834 Mitochondrial protease Lonp1 localizes to the nucleus in response to heat shock
Contributors : Marcello Pinti ; Lara Gibellini ; Rebecca Borella ; Mattia Forcato ; Giada ZaniniSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensWe analyzed the effects of Lonp1 silencing on the response to heat shock by analyzing the transriptomic profile of SW620 colon cancer cells undergoing heat shock. Briefly, Lonp1 has been silenced by using a specific siRNA, then cells have been kept at 42 °C for 1 hour, and left at 37°C for one hour.
Source: GEO: Gene Expression Omnibus - June 28, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE148795 Study of transcriptional effects of FOXS1 silencing and TGF-beta treatment in SNU-449 hepatocellular carcinoma cell line
Conclusions: FOXS1 is a novel TGFβ target and a key mediator of EMT. Its induction in cancer is associated with a poor prognosis. Thus, determining the exp ression of FOXS1 in advanced tumors in which the pro-metastatic arm of TGFβ is activated could help to identify patients who may benefit from targeted therapies using TGFβ inhibitors.
Source: GEO: Gene Expression Omnibus - June 15, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

GSE155231 RNA-seq data of prefoldin subunits PFDN2 and PFDN5 knockdowns in HCT116 human colon carcinoma cells
We report gene expression data from total RNA-seq of HCT116 human colon carcinoma cells treated with siRNA against PFDN2 and PFDN5 (both part of a complex known as prefoldin). Cells were independently treated with one of the previously mentioned siRNAs or with an exogenous control siRNA (luciferase GL2). All cells were treated with the different siRNAs for 24 hours, then serum-starved them for 48 hours, and finally stimulated by adding serum for 90 minutes. Samples were taken before and after serum stimulation. Prefoldin depletion provoked transcriptional alterations across the genome of human cells. Severe pre-mRNA splici...
Source: GEO: Gene Expression Omnibus - May 4, 2021 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

Overexpression of CASC11 in ovarian squamous cell carcinoma mediates the development of cancer cell resistance to chemotherapy.
Abstract LncRNA CASC11 promotes gastric cancer and colon cancer. Our study analyzed the role of CASC11 in ovarian squamous cell carcinoma (OSCC). In the present study we showed that plasma CASC11 was upregulated in OSCC, and the upregulation of CASC11 distinguished OSCC patients from control group. Plasma levels of CASC11 were further increased after chemotherapy. Treatment with oxaliplatin, tetraplatin, cisplatin, and carboplatin mediated the upregulation of CASC11 in cells of OSCC cell line. In addition, overexpression of CASC11 led to increased cancer cell viability under oxaliplatin, tetraplatin, cisplatin, an...
Source: Gene - June 6, 2019 Category: Genetics & Stem Cells Authors: Shen F, Feng L, Zhou J, Zhang H, Xu Y, Jiang R, Zhang H, Chen Y Tags: Gene Source Type: research

Molecular Mechanisms in Clear Cell Renal Cell Carcinoma: Role of miRNAs and Hypermethylated miRNA Genes in Crucial Oncogenic Pathways and Processes
Eleonora A. Braga1*†, Marina V. Fridman2†, Vitaly I. Loginov1,3, Alexey A. Dmitriev4 and Sergey G. Morozov1 1Institute of General Pathology and Pathophysiology, Moscow, Russia 2Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia 3Research Center of Medical Genetics, Moscow, Russia 4Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia Clear cell renal cell carcinoma (ccRCC) is the third most common urological cancer, and it has the highest mortality rate. The increasing drug resistance of metastatic ccRCC has resulted in the search f...
Source: Frontiers in Genetics - April 23, 2019 Category: Genetics & Stem Cells Source Type: research

GSE86172 BORIS knockdown in colorectal cancer cells HCT116 and Caco-2
Contributors : Yanmei Zhang ; Mengdie Fang ; Juan Ren ; Yongfei Song ; Haining Yuan ; Jianfei Fang ; Dongmei Yan ; Xiaoju WangSeries Type : Expression profiling by arrayOrganism : Homo sapiensBORIS expresses abnormally in colorectal cancer cells. Both the expression and the copy number of BORIS are remarkably higher in colorectal cancer cells than in normal colon or rectal cells. BORIS is potential diagnosis, prognosis or therapeutic target for colorectal cancer. To expand our view of the signaling pathway related with BORIS, altered gene expression by BORIS knockdown was assessed by microarray assay.To study the gene regu...
Source: GEO: Gene Expression Omnibus - July 1, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

ZFX acts as a transcriptional activator in multiple types of human tumors by binding downstream from transcription start sites at the majority of CpG island promoters RESEARCH
High expression of the transcription factor ZFX is correlated with proliferation, tumorigenesis, and patient survival in multiple types of human cancers. However, the mechanism by which ZFX influences transcriptional regulation has not been determined. We performed ChIP-seq in four cancer cell lines (representing kidney, colon, prostate, and breast cancers) to identify ZFX binding sites throughout the human genome. We identified roughly 9000 ZFX binding sites and found that most of the sites are in CpG island promoters. Moreover, genes with promoters bound by ZFX are expressed at higher levels than genes with promoters not...
Source: Genome Research - March 1, 2018 Category: Genetics & Stem Cells Authors: Rhie, S. K., Yao, L., Luo, Z., Witt, H., Schreiner, S., Guo, Y., Perez, A. A., Farnham, P. J. Tags: RESEARCH Source Type: research

ZFX acts as a transcriptional activator in multiple types of human tumors by binding downstream of transcription start sites at the majority of CpG island promoters RESEARCH
High expression of the transcription factor ZFX is correlated with proliferation, tumorigenesis, and patient survival in multiple types of human cancers. However, the mechanism by which ZFX influences transcriptional regulation has not been determined. We performed ChIP-seq in four cancer cell lines (representing kidney, colon, prostate, and breast cancers) to identify ZFX binding sites throughout the human genome. We identified ~9,000 ZFX binding sites and found that the majority of the sites are in CpG island promoters. Moreover, genes with promoters bound by ZFX are expressed at higher levels than genes with promoters n...
Source: Genome Research - February 2, 2018 Category: Genetics & Stem Cells Authors: Rhie, S. K., Yao, L., Luo, Z., Witt, H., Schreiner, S., Guo, Y., Perez, A. A., Farnham, P. J. Tags: RESEARCH Source Type: research

Transcriptome-wide targets of alternative splicing by RBM4 and possible role in cancer.
In conclusion, our findings reveal transcriptome-wide targets of RBM4 and point to potential cancer-related targets and mechanisms that may involve RBM4. PMID: 26898347 [PubMed - as supplied by publisher]
Source: Genomics - February 17, 2016 Category: Genetics & Stem Cells Authors: Markus MA, Yang YH, Morris BJ Tags: Genomics Source Type: research

Inhibition of {beta}-catenin signalling promotes DNA damage elicited by benzoapyrene in a model of human colon cancer cells via CYP1 deregulation
Deregulation of Wnt/β-catenin signalling plays an important role in the pathogenesis of colorectal cancer. Interestingly, this pathway has been recently implicated in transcriptional control of cytochrome P450 (CYP) family 1 enzymes, which are responsible for bioactivation of a number of dietary carcinogens. In the present study, we investigated the impact of inhibition of Wnt/β-catenin pathway on metabolism and genotoxicity of benzo[a]pyrene (BaP), a highly mutagenic polycyclic aromatic hydrocarbon and an efficient ligand of the aryl hydrocarbon receptor, which is known as a primary regulator of CYP1 expression,...
Source: Mutagenesis - June 16, 2015 Category: Genetics & Stem Cells Authors: Kabatkova, M., Zapletal, O., Tylichova, Z., Neca, J., Machala, M., Milcova, A., Topinka, J., Kozubik, A., Vondracek, J. Tags: Original Manuscript Source Type: research

Comprehensive analysis of beta-catenin target genes in colorectal carcinoma cell lines with deregulated Wnt/beta-catenin signaling
Conclusions: Based on the large number of potential beta-catenin target genes found to be similarly regulated in DLD1, SW480 and LS174T cells as well as the large overlap with confirmed beta-catenin target genes, we conclude that DLD1 and SW480 colon carcinoma cell lines are suitable model systems to study Wnt/beta-catenin signaling and associated colorectal carcinogenesis. Furthermore, the confirmed and the newly identified potential beta-catenin target genes are useful starting points for further studies.
Source: BMC Genomics - Latest articles - January 28, 2014 Category: Genetics & Stem Cells Authors: Andreas HerbstVindi JurinovicStefan KrebsSusanne ThiemeHelmut BlumBurkhard GökeFrank Kolligs Source Type: research