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Molecules, Vol. 28, Pages 4204: Triggering RNA Interference by Photoreduction under Red Light Irradiation
khir RNA interference (RNAi) using small interfering RNAs (siRNAs) is a powerful tool to target any protein of interest and is becoming more suitable for in vivo applications due to recent developments in RNA delivery systems. To exploit RNAi for cancer treatment, it is desirable to increase its selectivity, e.g., by a prodrug approach to activate the siRNAs upon external triggering, e.g., by using light. Red light is especially well suited for in vivo applications due to its low toxicity and higher tissue penetration. Known molecular (not nanoparticle-based) red-light-activatable siRNA prodrugs rely on singlet oxygen ...
Source: Molecules - May 20, 2023 Category: Chemistry Authors: Jennifer R ühle Insa Klemt Andriy Mokhir Tags: Article Source Type: research

Molecules, Vol. 27, Pages 4350: Melatonin Reverses the Warburg-Type Metabolism and Reduces Mitochondrial Membrane Potential of Ovarian Cancer Cells Independent of MT1 Receptor Activation
ustavo de Almeida Chuffa Ovarian cancer (OC) is the most lethal gynecologic malignancy, and melatonin has shown various antitumor properties. Herein, we investigated the influence of melatonin therapy on energy metabolism and mitochondrial integrity in SKOV-3 cells and tested whether its effects depended on MT1 receptor activation. SKOV-3 cells were exposed to different melatonin concentrations, and experimental groups were divided as to the presence of MT1 receptors (melatonin groups) or receptor absence by RNAi silencing (siRNA MT1+melatonin). Intracellular melatonin levels increased after treatment with melatonin in...
Source: Molecules - July 7, 2022 Category: Chemistry Authors: Maira Smaniotto Cucielo Roberta Carvalho Ces ário Henrique Spaulonci Silveira Let ícia Barbosa Gaiotte S érgio Alexandre Alcantara dos Santos Debora Aparecida Pires de Campos Zuccari F ábio Rodrigues Ferreira Seiva Russel J. Reiter Luiz Gustavo de Alm Tags: Article Source Type: research

Calcium-doped mesoporous silica nanoparticles as a lysosomolytic nanocarrier for amine-free loading and cytosolic delivery of siRNA
Publication date: 25 January 2020Source: Journal of Industrial and Engineering Chemistry, Volume 81Author(s): Eunshil Choi, Dong-Kwon Lim, Sehoon KimAbstractFor efficacious gene therapeutics, cytosolic transport of the endocytosed siRNA is crucial, not to mention a non-toxic delivery carrier composition. In this paper, we report facile achievement of amine-free loading and lysosomolytic delivery of siRNA in an unconventional way by using calcium (Ca2+)-doped mesoporous silica nanoparticles (CMSNs) as a host material along with a pore-loaded endosomal disruptor, chloroquine (CQ). It is demonstrated that CMSNs are capable of...
Source: Journal of Industrial and Engineering Chemistry - November 16, 2019 Category: Chemistry Source Type: research

Stress effects on FosB and interleukin-8 (IL8)-driven ovarian cancer growth and metastasis. Additions and Corrections
VOLUME 285 (2010) PAGES 35462–35470PAGE 35468:In Fig. 8C, the FosB siRNA-DOPC panels for no stress and stress were inadvertently duplicated. Additionally, the figure legend for Fig. 8C should read “CD31 (mouse endothelial cells are brown; tumor cell nuclei are blue).” These errors have now been corrected and do not affect the results or conclusions of this work.jbc;293/26/10041/FU1F1FU1
Source: Journal of Biological Chemistry - June 29, 2018 Category: Chemistry Authors: Mian M. K. Shahzad, Jesusa M. Arevalo, Guillermo N. Armaiz-Pena, Chunhua Lu, Rebecca L. Stone, Myrthala Moreno-Smith, Masato Nishimura, Jeong-Won Lee, Nicholas B. Jennings, Justin Bottsford-Miller, Pablo Vivas-Mejia, Susan K. Lutgendorf, Gabriel Lopez-Ber Tags: Additions and Corrections Source Type: research

Low concentrations of bisphenol A promote human ovarian cancer cell proliferation and glycolysis-based metabolism through the estrogen receptor- α pathway.
This study would thus open a new path to understand BPA-induced biological effects on tumor cells. PMID: 28709040 [PubMed - as supplied by publisher]
Source: Chemosphere - July 9, 2017 Category: Chemistry Authors: Shi XY, Wang Z, Liu L, Feng LM, Li N, Liu S, Gao H Tags: Chemosphere Source Type: research

Ectodomain shedding of the cell adhesion molecule Nectin-4 in ovarian cancer is mediated by ADAM10 and ADAM17 Membrane Biology
We report that ADAM17 and ADAM10 cleave Nectin-4 and release soluble Nectin-4 (sN4). Small molecule inhibitors and siRNA knockdown of both ADAM proteases confirmed these results. In matched samples from 11 high-grade serous ovarian cancer patients, we detected 2–20-fold more sN4 in ascites fluid than serum. Co-incubation of ovarian cancer cells with ascites fluid significantly increased sN4 shedding, which could be blocked using a dual inhibitor of ADAM10 and ADAM17. Furthermore, we detected RNA for Nectin-4, ADAM10, and ADAM17 in primary ovarian carcinoma tumors, secondary omental metastases, and ascites cells isolated ...
Source: Journal of Biological Chemistry - April 14, 2017 Category: Chemistry Authors: Petra C. Buchanan, Kristin L. M. Boylan, Bruce Walcheck, Rachel Heinze, Melissa A. Geller, Peter A. Argenta, Amy P. N. Skubitz Tags: Cell Biology Source Type: research

Characterization of H type 1 and type 1 N-acetyllactosamine glycan epitopes on ovarian cancer specifically recognized by the anti-glycan monoclonal antibody mAb-A4 Molecular Bases of Disease
Cancer-specific glycans of ovarian cancer are promising epitopes for targeting with monoclonal antibodies (mAb). Despite their potential, structural characterization of these glycan epitopes remains a significant challenge in mAb preclinical development. Our group generated the monoclonal antibody mAb-A4 against human embryonic stem cells (hESC), which also bound specifically to N-glycans present on 11 of 19 ovarian cancer (OC) and 8 of 14 breast cancer cell lines tested. Normal cell lines and tissue were unstained by mAb-A4. To characterize the N-linked glycan epitopes on OC cell lines targeted by mAb-A4, we used glycosid...
Source: Journal of Biological Chemistry - April 14, 2017 Category: Chemistry Authors: Matthew Choo, Heng Liang Tan, Vanessa Ding, Roberto Castangia, Omar Belgacem, Brian Liau, Lauren Hartley-Tassell, Stuart M. Haslam, Anne Dell, Andre Choo Tags: Glycobiology and Extracellular Matrices Source Type: research

Proteomic Delineation of Ovarian Cancer Cell Line Aggregates Genomics and Proteomics
Ovarian cancer is a lethal gynecological disease that is characterized by peritoneal metastasis and increased resistance to conventional chemotherapies. This increased resistance and the ability to spread is often attributed to the formation of multicellular aggregates or spheroids in the peritoneal cavity, which seed abdominal surfaces and organs. Given that the presence of metastatic implants is a predictor of poor survival, a better understanding of how spheroids form is critical to improving patient outcome, and may result in the identification of novel therapeutic targets. Thus, we attempted to gain insight into the p...
Source: Journal of Biological Chemistry - July 9, 2015 Category: Chemistry Authors: Musrap, N., Tuccitto, A., Karagiannis, G. S., Saraon, P., Batruch, I., Diamandis, E. P. Tags: Genomics and Proteomics Source Type: research

p53 Is Required for Mitochondrial Fragmentation Cell Biology
Mitochondria are highly dynamic organelles, and mitochondrial fission is a crucial step of apoptosis. Although Oma1 is believed to be responsible for long form Opa1 (L-Opa1) processing during mitochondrial fragmentation, whether and how Oma1 is involved in L-Opa1 processing and participates in the regulation of chemoresistance is unknown. Chemosensitive and chemoresistant ovarian (OVCA) and cervical (CECA) cancer cells were treated with cisplatin (CDDP). Mitochondrial dynamics and protein contents were assessed by immunofluorescence and Western blot, respectively. The requirements of Oma1 and p53 for CDDP-induced L-Opa1 pr...
Source: Journal of Biological Chemistry - September 25, 2014 Category: Chemistry Authors: Kong, B., Wang, Q., Fung, E., Xue, K., Tsang, B. K. Tags: Molecular Bases of Disease Source Type: research

Autophagy and Cisplatin Resistance Signal Transduction
Cisplatin-based treatment is the first line chemotherapy for several cancers including ovarian cancer. The development of cisplatin resistance results in treatment failure, but the underlying mechanisms are not fully understood. Here we show that the induction of autophagy plays an important role in cisplatin resistance in ovarian cancer cells. Specifically, we show that cisplatin resistance is correlated with autophagy induction in a panel of ovarian cancer cells but not in immortalized human ovarian surface epithelial cells. Mechanistically, cisplatin treatment activates ERK and subsequently promotes autophagy. The inhib...
Source: Journal of Biological Chemistry - June 12, 2014 Category: Chemistry Authors: Wang, J., Wu, G. S. Tags: Molecular Bases of Disease Source Type: research

Pyrido2,3-dpyrimidines: Discovery and preliminary SAR of a novel series of DYRK1B and DYRK1A inhibitors.
Abstract DYRK1B is a kinase over-expressed in certain cancer cells (including colon, ovarian, pancreatic, etc.). Recent publications have demonstrated inhibition of DYRK1B could be an attractive target for cancer therapy. From a data-mining effort, the team has discovered analogues of pyrido[2,3-d]pyrimidines as potent enantio-selective inhibitors of DYRK1B. Cells treated with a tool compound from this series showed the same cellular effects as down regulation of DYRK1B with siRNA. Such effects are consistent with the proposed mechanism of action. Progress of the SAR study is presented. PMID: 24239188 [PubMed...
Source: Bioorganic and Medicinal Chemistry Letters - November 1, 2013 Category: Chemistry Authors: Anderson K, Chen Y, Chen Z, Dominique R, Glenn K, He Y, Janson C, Luk KC, Lukacs C, Polonskaia A, Qiao Q, Railkar A, Rossman P, Sun H, Xiang Q, Vilenchik M, Wovkulich P, Zhang X Tags: Bioorg Med Chem Lett Source Type: research

Novel Roles of Mitochondrial Uniporter in Ovarian Cancer Cell Biology
This study highlights the potential of nanomaterials as a tool to broaden our understanding of cellular processes, establishes MICU1 as a novel regulator of the machinery in cancer cells that prevents apoptosis, and emphasizes the need to synergize nanoparticle design with understanding of mitochondrial machinery for enhancing targeted cellular toxicity.
Source: Journal of Biological Chemistry - June 14, 2013 Category: Chemistry Authors: Arvizo, R. R., Moyano, D. F., Saha, S., Thompson, M. A., Bhattacharya, R., Rotello, V. M., Prakash, Y. S., Mukherjee, P. Tags: Signal Transduction Source Type: research