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Total 16445 results found since Jan 2013.

Current Development of siRNA Bioconjugates: From Research to the Clinic
In this study, it was shown that the main factor determining the nature of the biodistribution of conjugates is their lipophilicity. Conjugates of siRNA with lower lipophilicity; i.e., derivatives of retinoic acid, lithocholic acid, and docosahexanoic acid with greater efficiency than cholesterol conjugates accumulated in the kidneys, bladder, and lungs of the mouse after subcutaneous injection (Biscans et al., 2018). This fact is consistent with previous data that showed that more lipophilic conjugates bind more efficiently to serum components, and thus are not excreted by the kidneys (Wolfrum et al., 2007; Osborn et al.,...
Source: Frontiers in Pharmacology - April 25, 2019 Category: Drugs & Pharmacology Source Type: research

Targeted siRNA-immunoliposomes as a promising therapeutic agent against highly pathogenic avian influenza A (H5N1) virus infection.
This study describes a proof of concept study on the use of siRNA-immunoliposomes as a therapeutic agent against H5N1 influenza virus infection. SiRNA specific for influenza virus nucleoprotein (NP) mRNA was employed as the key antiviral agent to inhibit viral replication in this study. A humanized single-chain Fv antibody (huscFv) against the hemagglutinin (HA) of H5N1 highly pathogenic avian influenza (HPAI) was used as the targeting molecule to HA of H5N1 virus, which is abundantly expressed on the surface of infected cells. The huscFv was decorated onto cationic PEGylated DC-Chol/DOPE liposomes to generate immunoliposo...
Source: Antimicrobial Agents and Chemotherapy - March 10, 2014 Category: Microbiology Authors: Khantasup K, Kopermsub P, Chaichoun K, Dharakul T Tags: Antimicrob Agents Chemother Source Type: research

Scoping Review on Search Queries and Social Media for Disease Surveillance: A Chronology of Innovation
Conclusions: The use of search queries and social media for disease surveillance are relatively recent phenomena (first reported in 2006). Both the tools themselves and the methodologies for exploiting them are evolving over time. While their accuracy, speed, and cost compare favorably with existing surveillance systems, the primary challenge is to refine the data signal by reducing surrounding noise. Further developments in digital disease surveillance have the potential to improve sensitivity and specificity, passively through advances in machine learning and actively through engagement of users. Adoption, even as suppor...
Source: Journal of Medical Internet Research - July 18, 2013 Category: Journals (General) Authors: Theresa Marie BernardoAndrijana RajicIan YoungKatie RobiadekMai T PhamJulie A Funk Source Type: research

Lysosomal escaped protein nanocarriers for nuclear-targeted siRNA delivery
Anal Bioanal Chem. 2021 Mar 26. doi: 10.1007/s00216-021-03297-5. Online ahead of print.ABSTRACTIn the process of drug carrier design, lysosome degradation in cells is often neglected, which makes a considerable number of drugs not play a role. Here, we have constructed a tumor treatment platform (Apn/siRNA/NLS/HA/Apt) with unique lysosomal escape function and excellent cancer treatment effect. Apoferritin (Apn) has attracted more and more attention because of its high uniformity, modifiability, and controllability. Meanwhile, its endogenous nature can avoid the risk of immune response being eliminated. We used aptamer modi...
Source: Analytical and Bioanalytical Chemistry - March 26, 2021 Category: Chemistry Authors: Xiuping Cao Xinxin Shang Yingshu Guo Xiaofei Zheng Wenxin Li Di Wu Li Sun Shanliang Mu Chuanen Guo Source Type: research

Conjugates of HA2 with octaarginine-grafted HPMA copolymer offer effective siRNA delivery and gene silencing in cancer cells.
Abstract The key for successful gene silencing is to design a safe and efficient siRNA delivery system for the transfer of therapeutic nucleic acids into the target cells. Here, we describe the design of hydrophilic N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer displaying multiple copies of octaarginine (R8) and its use in promoting the effective delivery of small interfering RNA (siRNA) molecules intracellularly. Fluorescein-5-isothiocyanate (FITC)-labeled HPMA copolymer-bound R8 (P-R8-FITC) was synthesized with increasing R8 molar ratios (4 to 9.5 mol-%) to define the optimal R8 content that allowed the pol...
Source: European Journal of Pharmaceutics and Biopharmaceutics - September 30, 2016 Category: Drugs & Pharmacology Authors: Golan M, Feinshtein V, David A Tags: Eur J Pharm Biopharm Source Type: research

Bifunctional siRNA containing immunostimulatory motif 2 enhances protection against pandemic H1N1 virus infection.
CONCLUSIONS: This study paves the way for broad-spectrum RNAi-based therapeutics using immunostimulatory motif towards improved antiviral effect. Hence this approach will be useful to confront the sudden emergence of pandemic strains. PMID: 26264705 [PubMed - as supplied by publisher]
Source: Current Gene Therapy - August 12, 2015 Category: Genetics & Stem Cells Authors: Joshi G, Dash PK, Agarwal A, Sharma S, Parida M Tags: Curr Gene Ther Source Type: research

Polymer Nanocarrier System for Endosome Escape and Timed Release of siRNA with Complete Gene Silencing and Cell Death in Cancer Cells.
Abstract An influenza virus-inspired polymer mimic nanocarrier was used to deliver siRNA for specific and near complete gene knockdown of an osteoscarcom cell line (U-2SO). The polymer was synthesized by single-electron transfer living radical polymerization (SET-LRP) at room temperature to avoid complexities of transfer to monomer or polymer. It was the only LRP method that allowed good block copolymer formation with a narrow molecular weight distribution. At nitrogen to phosphorus (N/P) ratios of equal to or greater than 20 (greater than a polymer concentration of 13.8 μg/mL) with polo-like kinase 1 (PLK1) siRN...
Source: Biomacromolecules - September 4, 2013 Category: Biochemistry Authors: Gu W, Jia Z, Truong NP, Prasadam I, Xiao Y, Monteiro MJ Tags: Biomacromolecules Source Type: research

Optimised design of siRNA based on multi-featured comparison and analysis of H1N1 virus.
In this study, we developed a computational method for designing therapeutics siRNA and applied in the recent HIN1 influenza virus based on its biological characteristics that are substantially different from seasonal influenza virus. We first compared the PA fragments between the H IN1 virus in 2009 and the seasonal influenza virus genes in 2008, and the comparison found significant differences between them not only in sequence features but also in RNA secondary structures. In particular, the RNA secondary structures only share 76.8% identity, which suggests major changes in biological characteristics. Furthermore, we des...
Source: International Journal of Data Mining and Bioinformatics - June 29, 2013 Category: Bioinformatics Authors: Liu Y, Chang Y, Xu D, Li Z, Zhang H, Li J, Tian M Tags: Int J Data Min Bioinform Source Type: research

Combination of specific single chain antibody variable fragment and siRNA has a synergistic inhibitory effect on the propagation of avian influenza virus H5N1 in chicken cells
Conclusions: A novel combination of antiviral siRNAs expressed in chicken cells and chicken antibody single-chain variable fragments (scFvs) secreted from the cells has a synergistic inhibitory effect on the avian influenza viral proliferation in vitro. Intracellular application of scFvs and anti-viral siRNA may provide a new approach to influenza prevention and treatment.
Source: Virology Journal - November 29, 2014 Category: Virology Authors: Shuang WangPeng ZhangFei HeJi-Gui WangJia-Zeng SunZhi-Li LiBao YiJi XiYa-Ping MaoQiang HouDao-Li YuanZi-Ding ZhangWei-Quan Liu Source Type: research

GSE66661 Analysis of global gene expression profiles of A549 cells treated with Xrn2 siRNA upon Influenza A/PR/8/34(ΔNS1) Infection
Contributors : Matthew S Miller ; Alexander Rialdi ; Jessica S Ho ; Micah Tilove ; Luis Martinez-Gil ; Natasha P Moshkina ; Zuleyma Peralta ; Justine Noel ; Camilla Melegari ; Ana Maestre ; Panagiotis Mitsopoulos ; Joaquín Madrenas ; Sven Heinz ; Chris Benner ; John A Young ; Alicia R Feagins ; Christopher F Basler ; Ana Fernandez-Sesma ; Olivier J Bequerel ; Martin F Lavin ; Harm van Bakel ; Ivan MarazziSeries Type : Expression profiling by arrayOrganism : Homo sapiensUntransfected (no siRNA), control siRNA or Xrn2 siRNA treated A549 cells were infected with A/Puerto Rico/8/1934(ΔNS1) for 4 hours
Source: GEO: Gene Expression Omnibus - March 30, 2015 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

GSE52932 Analysis of global gene expression profiles of A549 cells treated with SETX siRNA upon Influenza A/PR/8/34(ΔNS1) Infection
Contributors : Matthew S Miller ; Alexander Rialdi ; Jessica S Ho ; Micah Tilove ; Luis Martinez-Gil ; Natasha P Moshkina ; Zuleyma Peralta ; Justine Noel ; Camilla Melegari ; Ana M Maestre ; Panagiotis Mitsopoulos ; Joaquín Madrenas ; Sven Heinz ; Chris Benner ; John A Young ; Alicia R Feagins ; Christopher F Basler ; Ana Fernandez-Sesma ; Olivier J Bequerel ; Martin F Lavin ; Harm van Bakel ; Ivan MarazziSeries Type : Expression profiling by arrayOrganism : Homo sapiensUntransfected (no siRNA), control siRNA or SETX siRNA treated A549 cells were infected with A/Puerto Rico/8/1934(ΔNS1) for 4 hours
Source: GEO: Gene Expression Omnibus - March 30, 2015 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

Expression of a single siRNA against a conserved region of NP gene strongly inhibits in vitro replication of different Influenza A virus strains of avian and swine origin.
In conclusion, these findings reveal new siRNA sequences able to inhibit Influenza A virus replication and provide a basis for the development of siRNAs as prophylaxis and therapy for influenza infection both in humans and animals. PMID: 25986248 [PubMed - as supplied by publisher]
Source: Antiviral Research - May 16, 2015 Category: Virology Authors: Stoppani E, Bassi I, Dotti S, Lizier M, Ferrari M, Lucchini F Tags: Antiviral Res Source Type: research