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Specialty: Biochemistry

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Total 111164 results found since Jan 2013.

Assessment of the coordinated role of ST3GAL3, ST3GAL4 and ST3GAL6 on the α 2,3 sialylation linkage of mammalian glycoproteins.
Abstract In this research, we examined which genes are involved in N-linked sialylation in Chinese Hamster Ovary (CHO) cells using siRNA knockdown approaches. Three genes from the sialyltransferase family (ST3GAL3, ST3GAL4 and ST3GAL6) were chosen as knockdown targets with siRNA applied to reduce their expression. Single, double and triple gene knockdowns were investigated, and the reduction levels of sialylation on the total cell lysate were monitored by enzyme-linked lectin absorption assays (ELLA) and sialic acid quantification with high performance liquid chromatography (HPLC). All transfection groups showed e...
Source: Biochemical and Biophysical Research communications - May 18, 2015 Category: Biochemistry Authors: Chung CY, Yin B, Wang Q, Chuang KY, Chu JH, Betenbaugh MJ Tags: Biochem Biophys Res Commun Source Type: research

Development of Guanidinium-rich Protein Mimics for Efficient siRNA Delivery into Human T Cells.
Abstract RNA interference is gaining attention as a means to explore new molecular pathways and for its potential as a therapeutic; however, its application in immortal and primary T cells is limited due to challenges with efficient delivery in these cells types. Herein, we report the development of guanidinium-rich protein transduction domain mimics (PTDMs) based on a ring-opening metathesis polymerization scaffold that delivers siRNA into Jurkat T cells and human peripheral blood mononuclear cells (hPBMCs). Homopolymer and block copolymer PTDMs with varying numbers of guanidinium moieties were designed and teste...
Source: Biomacromolecules - August 31, 2015 Category: Biochemistry Authors: deRonde BM, Torres JA, Minter LM, Tew GN Tags: Biomacromolecules Source Type: research

Folate Receptor Targeted Delivery of siRNA and Paclitaxel to Ovarian Cancer Cells via Folate Conjugated Triblock Co-polymer to Overcome TLR4 Driven Chemotherapy Resistance.
This study demonstrates that PEI-g-PCL-b-PEG-Fol conjugates are a reliable delivery system for siRNA and are able to mediate therapeutic protein knockdown within ovarian cancer cells. Additionally, this study provides further evidence to link TLR4 levels to chemotherapy resistance. PMID: 26636884 [PubMed - as supplied by publisher]
Source: Biomacromolecules - December 4, 2015 Category: Biochemistry Authors: Jones SK, Lizzio V, Merkel O Tags: Biomacromolecules Source Type: research

Optimal Hydrophobicity in ROMP-based Protein Mimics Required for siRNA Internalization.
Abstract Exploring the role of polymer structure for the internalization of biologically relevant cargo, specifically siRNA, is of critical importance to the development of improved delivery reagents. Herein, we report guanidinium-rich protein transduction domain mimics (PTDMs) based on a ring-opening metathesis polymerization scaffold containing tunable hydrophobic moieties that promote siRNA internalization. Structure-activity relationships using Jurkat T cells and HeLa cells were explored to determine how the length of the hydrophobic block and the hydrophobic side chain compositions of these PTDMs impacted siR...
Source: Biomacromolecules - April 20, 2016 Category: Biochemistry Authors: deRonde BM, Posey ND, Otter R, Caffrey LM, Minter LM, Tew GN Tags: Biomacromolecules Source Type: research

Dual-functionalized graphene oxide for enhanced siRNA delivery to breast cancer cells
Publication date: 1 November 2016 Source:Colloids and Surfaces B: Biointerfaces, Volume 147 Author(s): Rana Imani, Wei Shao, Samira Taherkhani, Shahriar Hojjati Emami, Satya Prakash, Shahab Faghihi The aim of this study is to improve hydrocolloid stability and siRNA transfection ability of a reduced graphene oxide (rGO) based nano-carrier using a phospholipid-based amphiphilic polymer (PL-PEG) and cell penetrating peptide (CPPs). The dual functionalized nano-carrier is comprehensively characterized for its chemical structure, size, surface charge and morphology as well as thermal stability. The nano-carrier cytocompatibil...
Source: Colloids and Surfaces B: Biointerfaces - August 17, 2016 Category: Biochemistry Source Type: research

Lipophilic 2'-O-Acetalester RNAs:Synthesis, Thermal Duplex Stability, Nuclease Resistance, Cellular Uptake and siRNA Activity after Spontaneous Naked Delivery.
We report synthesis, biochemical and biophysical characteristics of 2'-O-phenylisobutyryloxymethyl (PiBuOM)-modified siRNA and their impact on biological activity. In case of spontaneous cellular uptake of naked PiBuOM-modified siRNA, we observe increased target suppression in human cells versus unmodified or pivaloyloxymethyl (PivOM)-modified siRNA. We provide evidence for improved spontaneous cellular uptake of naked PiBuOM-modified siRNA and for substantial target suppression in human cells in serum-containing medium. PMID: 27569765 [PubMed - as supplied by publisher]
Source: Chembiochem - August 28, 2016 Category: Biochemistry Authors: Biscans A, Bertrand JR, Dubois J, Rüger J, Vasseur JJ, Sczakiel G, Dupouy C, Debart F Tags: Chembiochem Source Type: research

Mapping Optimal Charge Density and Length of ROMP-based PTDMs for siRNA Internalization.
Abstract A fundamental understanding of how polymer structure impacts internalization and delivery abilities of biologically relevant cargoes, particularly siRNA, is of critical importance to the successful design of improved delivery reagents. Herein we report the use of ring-opening metathesis polymerization (ROMP) methods to synthesize two series of guanidinium-rich protein transduction domain mimics (PTDMs): one based on an imide scaffold that contains one guanidinium moiety per repeat unit and another based on a diester scaffold that contains two guanidinium moieties per repeat unit. By varying both the degre...
Source: Biomacromolecules - September 5, 2016 Category: Biochemistry Authors: Caffrey LM, deRonde BM, Minter LM, Tew GN Tags: Biomacromolecules Source Type: research

Recent advances in mechanism-based chemotherapy drug-siRNA pairs in co-delivery systems for cancer: A review
Publication date: 1 September 2017 Source:Colloids and Surfaces B: Biointerfaces, Volume 157 Author(s): Mingfang Wang, Jinyu Wang, Bingcheng Li, Lingxin Meng, Zhaoxing Tian Co-delivery of chemotherapy drugs and siRNA for cancer therapy has achieved remarkable results according to synergistic/combined antitumor effects, and is recognized as a promising therapeutic modality. However, little attention has been paid to the extremely complex mechanisms of chemotherapy drug-siRNA pairs during co-delivery process. Proper selection of chemotherapy drug-siRNA pairs is beneficial for achieving desirable cancer therapeutic effects. ...
Source: Colloids and Surfaces B: Biointerfaces - June 12, 2017 Category: Biochemistry Source Type: research

Synthesis and characterization of amino acid-functionalized calcium phosphate nanoparticles for siRNA delivery
Publication date: 1 October 2017 Source:Colloids and Surfaces B: Biointerfaces, Volume 158 Author(s): Feray Bakan, Goknur Kara, Melike Cokol Cakmak, Murat Cokol, Emir Baki Denkbas Small interfering RNAs (siRNA) are short nucleic acid fragments of about 20–27 nucleotides, which can inhibit the expression of specific genes. siRNA based RNAi technology has emerged as a promising method for the treatment of a variety of diseases. However, a major limitation in the therapeutic use of siRNA is its rapid degradation in plasma and cellular cytoplasm, resulting in short half-life. In addition, as siRNA molecules cannot penetrate...
Source: Colloids and Surfaces B: Biointerfaces - July 8, 2017 Category: Biochemistry Source Type: research

Additive polyplexes to undertake siRNA therapy against CDC20 and survivin in breast cancer cells.
Abstract Small interfering RNA (siRNA) delivered to silence overexpressed genes associated with malignancies is a promising targeted therapy to decrease the uncontrolled growth of malignant cells. To create potent delivery agents for siRNA, here we formulated additive polyplexes of siRNA using linoleic acid-substituted polyethylenimine and additive polymers (hyaluronic acid, polyacrylic acid, dextran sulfate and methyl cellulose), and characterized their physicochemical properties and effectiveness. Incorporating polyanionic polymer along with anionic siRNA in polyplexes was found to decrease the ζ-potential of p...
Source: Biomacromolecules - September 17, 2018 Category: Biochemistry Authors: Parmar MB, K C RB, Löbenberg R, Uludag H Tags: Biomacromolecules Source Type: research

Pyruvate responsiveness based on α-oxohydrazone formation for intracellular siRNA release from polyion complex (PIC)-based carriers.
This study provides the rationale for a smart design of pyruvate-responsive materials in the cell, based on α-oxohydrazone formation. PMID: 31091092 [PubMed - as supplied by publisher]
Source: Biomacromolecules - May 14, 2019 Category: Biochemistry Authors: Takemoto H, Wang CL, Nomoto T, Matsui M, Tomoda K, Nishiyama N Tags: Biomacromolecules Source Type: research

Design of curdlan-based pH-sensitive polymers with endosome buffering functionality for siRNA delivery.
Abstract Developing nucleic acid-based tools to control disease-relevant gene expression in human disorders, such as siRNAs, opens up potential opportunities for therapeutics. Because of their high molecular weight and polyanionic nature, synthetic siRNAs fail to cross biological membranes by passive diffusion and therefore, generally require transmembrane siRNA delivery technologies to access the cytoplasm of target cells. To create a biocompatible siRNA delivery agent, we chemically modified natural polysaccharide curdlan derivative 6AC-100 in a regioselective manner to introduce different ratios of imidazole ri...
Source: International Journal of Biological Macromolecules - November 24, 2019 Category: Biochemistry Authors: Su Z, Erdene-Ochir T, Ganbold T, Baigude H Tags: Int J Biol Macromol Source Type: research

Hyperbranched cationic polysaccharide derivatives for efficient siRNA delivery and diabetic wound healing enhancement.
Abstract Gene vectors are important for successful siRNA delivery. Four types of hyperbranched cationic polysaccharide derivatives (HCP) were synthesized by conjuncting 1,2-ethylenediamine (EDA) and diethylenetriamine (DETA) with glycogen or amylopectin respectively and named as G-EDA, G-DETA, A-EDA and A-DETA. The efficiency and safety of these HCPs to deliver siRNA were explored in vitro and in vivo. Our results showed that HCPs could form complexes with siRNA. All HCP/siRNA exhibited negligible cytotoxicity. Compared with A-EDA and A-DETA, G-EDA and G-DETA could carry much more siRNA into cells and then escape ...
Source: International Journal of Biological Macromolecules - March 16, 2020 Category: Biochemistry Authors: Lan B, Wu J, Li N, Pan C, Yan L, Yang C, Zhang L, Yang L, Ren M Tags: Int J Biol Macromol Source Type: research

Recent Advances of Polycationic siRNA Vectors for Cancer Therapy.
Abstract Small interfering RNAs (siRNAs) have recently emerged as a new class of biopharmaceuticals for the treatment of various diseases, including genetic diseases, viral infections, heritable disorders, and most prominently, cancer. However clinical applications of siRNA-based therapeutics through intravenous administration have been limited due to their rapid degradation and renal clearance, poor cellular uptake, low cytoplasmic release by escaping endocytic uptake and off-target effects. The success of siRNA-based therapeutics depends upon the design and creation of efficient delivery vectors that should be a...
Source: Biomacromolecules - June 21, 2020 Category: Biochemistry Authors: Bholakant R, Qian H, Zhang J, Huang X, Huang D, Feijen J, Zhong Y, Chen W Tags: Biomacromolecules Source Type: research

PLGA nanoparticles containing α-fetoprotein siRNA induce apoptosis and enhance the cytotoxic effects of doxorubicin in human liver cancer cell line
In this study, the siRNA targeting α-fetoprotein (AFP) mRNA was found to specifically induce apoptosis and significant cell death in HepG2 cells. It also enhanced the cytotoxic effects of doxorubicin by about two-fold, making it the candidate therapeutic molecule for HCC treatment. To deliver the siRNAs into HCC cells, the AFP siRNAs were loaded into the nanoparticles based on poly (lactic-co-glycolic) acid (PLGA). These nanoparticles induced apoptosis in HepG2 cells and synergistically increased the cytotoxicity of doxorubicin. In summary, the delivery of the AFP siRNA-loaded PLGA nanoparticles in combination with doxoru...
Source: Biochemical and Biophysical Research communications - March 28, 2021 Category: Biochemistry Authors: Theeraphong Pho-Iam Primana Punnakitikashem Chayapol Somboonyosdech Sirinapa Sripinitchai Patarabutr Masaratana Vorapan Sirivatanauksorn Yongyut Sirivatanauksorn Chamaiphorn Wongwan Kytai T Nguyen Chatchawan Srisawat Source Type: research