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Infectious Disease: Gastroenteritis
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Total 36193 results found since Jan 2013.
Vacuolar localisation of anthocyanin pigmentation in microgreen cotyledons of basil, cabbage and mustard greens does not impact on colonisation by Shiga-toxigenic Escherichia coli O157:H7
Food Microbiol. 2023 Dec;116:104367. doi: 10.1016/j.fm.2023.104367. Epub 2023 Aug 21.ABSTRACTMicrogreens, the immature plants harvested after a few weeks of growth, are perceived as a heathy, nutritious food ingredient but may be susceptible to colonisation by human pathogens including Shiga-toxigenic Escherichia coli (STEC). Some microgreen cultivars accumulate anthocyanins or secrete essential oils which, when extracted or purified, have been reported to inhibit bacterial growth. Therefore, the impact of anthocyanins on bacterial colonisation by STEC (Sakai) was compared for three species that have pigmented cultivars: b...
Source: Food Microbiology - September 9, 2023 Category: Food Science Authors: Kathryn M Wright Jacqueline Marshall Peter J Wright Nicola J Holden Source Type: research
Prevalence of ciprofloxacin resistance and associated genetic determinants differed among Campylobacter isolated from human and poultry meat sources in Pennsylvania
Food Microbiol. 2023 Dec;116:104349. doi: 10.1016/j.fm.2023.104349. Epub 2023 Aug 3.ABSTRACTPoultry is the primary source of Campylobacter infections and severe campylobacteriosis cases are treated with macrolides and fluoroquinolones. However, these drugs are less effective against antimicrobial-resistant strains. Here, we investigated the prevalence of phenotypic antimicrobial resistance and associated resistance genetic determinants in Campylobacter isolates collected from human clinical (N = 123) and meat (N = 80) sources in Pennsylvania in 2017 and 2018. Our goal was to assess potential differences in the prevalence o...
Source: Food Microbiology - September 9, 2023 Category: Food Science Authors: Runan Yan Nkuchia M M'ikanatha Irving Nachamkin Lauren K Hudson Thomas G Denes Jasna Kovac Source Type: research
Detection of resistance and virulence plasmids in Campylobacter coli and Campylobacter jejuni isolated from North Carolina food animal production, 2018-2019
Food Microbiol. 2023 Dec;116:104348. doi: 10.1016/j.fm.2023.104348. Epub 2023 Aug 11.ABSTRACTCampylobacter remains the leading cause of bacterial foodborne illness in the U.S. and worldwide. Campylobacter plasmids may play a significant role in antimicrobial resistance (AMR) and virulence factor distribution, and potentially drive rapid adaptation. C. coli (n = 345) and C. jejuni (n = 199) isolates collected from live cattle, swine, turkey, and chickens, poultry carcasses at production, and retail meat in N.C. were analyzed to determine plasmid prevalence, extrachromosomal virulence and AMR genes, and the phylogeny of asse...
Source: Food Microbiology - September 9, 2023 Category: Food Science Authors: Dawn M Hull Erin Harrel Lyndy Harden Siddhartha Thakur Source Type: research
The efficiency of UV light-emitting diodes (UV-LED) in decontaminating Campylobacter and Salmonella and natural microbiota in chicken breast, compared to a UV pilot-plant scale device
This study investigated the combined effect of Ultraviolet (UV) light-emitting diode (LED) technology treatment with refrigerated storage of chicken breast meat over 7 days on Campylobacter jejuni, Salmonella enterica serovar Typhimurium, total viable counts (TVC) and total Enterobacteriaceae counts (TEC). An optimised UV-LED treatment at 280 nm for 6 min decreased inoculated S. Typhimurium and C. jejuni populations by 0.6-0.64 log CFU/g, and TVC and TEC population by 1-1.2 log CFU/g in chicken samples. During a 7-day storage at 4 °C, a 0.73 log reduction in C. jejuni was achieved compared with non-treated samples. Moreov...
Source: Food Microbiology - September 9, 2023 Category: Food Science Authors: Arturo B Soro Daniel Ekhlas Sajad Shokri Ming Ming Yem Rui Chao Li Soukaina Barroug Shay Hannon Paul Whyte Declan J Bolton Catherine M Burgess Paula Bourke Brijesh K Tiwari Source Type: research
Modelling norovirus dynamics within oysters emphasises potential food safety issues associated with current testing & amp; depuration protocols
Food Microbiol. 2023 Dec;116:104363. doi: 10.1016/j.fm.2023.104363. Epub 2023 Aug 18.ABSTRACTNorovirus is a significant global cause of viral gastroenteritis, with raw oyster consumption often linked to such outbreaks due to their filter-feeding in harvest waters. National water quality and depuration/relaying times are often classified using Escherichia coli, a poor proxy for norovirus levels in shellfish. The current norovirus assay is limited to only the digestive tracts of oysters, meaning the total norovirus load of an oyster may differ from reported results. These limitations motivated this work, building upon previo...
Source: Food Microbiology - September 9, 2023 Category: Food Science Authors: Paul McMenemy Adam Kleczkowski Nick G H Taylor Source Type: research
Ability of Latilactobacillus curvatus FAM25164 to produce tryptamine: Identification of a novel tryptophan decarboxylase
This study describes a novel bacterial tryptophan decarboxylase (EC 4.1.1.105) that may be responsible for tryptamine formation in cheese.PMID:37689414 | DOI:10.1016/j.fm.2023.104343
Source: Food Microbiology - September 9, 2023 Category: Food Science Authors: Stefan Irmler Tharmatha Bavan Eliane Binz Reto Portmann Source Type: research
Unsaturated fatty acid synthesis in bacteria: Mechanisms and regulation of canonical and remarkably noncanonical pathways
Biochimie. 2023 Sep 6:S0300-9084(23)00216-X. doi: 10.1016/j.biochi.2023.09.007. Online ahead of print.ABSTRACTUnsaturated phospholipid acyl chains are required for membrane function in most bacteria. The double bonds of the cis monoenoic chains arise by two distinct pathways depending on whether oxygen is required. The oxygen-independent pathway (traditionally called the anaerobic pathway) introduces the cis double bond by isomerization of the trans double bond intermediate of the fatty acid elongation cycle. Double bond isomerization occurs at an intermediate chain length (e.g., C10) and the isomerization product is elong...
Source: Biochimie - September 8, 2023 Category: Biochemistry Authors: John E Cronan Source Type: research
DamID-seq: A Genome-Wide DNA Methylation Method that Captures Both Transient and Stable TF-DNA Interactions in Plant Cells
Methods Mol Biol. 2023;2698:87-107. doi: 10.1007/978-1-0716-3354-0_7.ABSTRACTCapturing the dynamic and transient interactions of a transcription factor (TF) with its genome-wide targets whose regulation leads to plants' adaptation to their changing environment is a major technical challenge. This is a widespread problem with biochemical methods such as chromatin immunoprecipitation-sequencing (ChIP-seq) which are biased towards capturing stable TF-target gene interactions. Herein, we describe how DNA adenine methyltransferase identification and sequencing (DamID-seq) can be used to capture both transient and stable TF-targ...
Source: Mol Biol Cell - September 8, 2023 Category: Molecular Biology Authors: Jos é M Alvarez Will E Hinckley Lauriebeth Leonelli Matthew D Brooks Gloria M Coruzzi Source Type: research
Interventional effects of oral microecological agents on perioperative indicators of colorectal cancer: a meta-analysis
CONCLUSION: By increasing the number of beneficial flora such as Lactobacillus and Bifidobacterium and decreasing the number of harmful flora such as Escherichia coli, the micro-ecological preparation group is beneficial in improving the ecological dysregulation in colorectal cancer patients receiving different treatments in the perioperative period. The microecological preparation group was able to reduce many types of adverse drug reactions, such as infections and gastrointestinal discomfort, compared to the control group. The microecological agents also reduced inflammatory responses, decreased the increase in harmful m...
Source: Cancer Control - September 8, 2023 Category: Cancer & Oncology Authors: Xueyan Wang Lijun Pan Feiqing Wang Fengxi Long Bing Yang Dongxin Tang Source Type: research
Selection for antimicrobial prophylaxis in emergency and elective transurethral procedures: Susceptibility pattern in T ürkiye
CONCLUSION: We found that currently recommended antimicrobials provide poor coverage for the most common pathogens isolated. Urologists should consider patient-based antibiotic prophylaxis in endoscopic urethral procedures, follow appropriate proto-cols, and consider local antibiotic resistance.PMID:37681729 | DOI:10.14744/tjtes.2023.99663
Source: Turkish Journal of Trauma and Emergency Surgery : TJTES - September 8, 2023 Category: Emergency Medicine Authors: Tanju Keten Melih Balci Ünsal Eroğlu Ali Yasin Özercan Şeref Coşer Serdar Ba şboğa Koray Tatl ıcı Anil Erkan Çağdaş Şenel Remzi Salar Özer Güzel Y ılmaz Aslan Altug Tuncel Ali Atan Source Type: research
A soft multifunctional film from chitosan modified with disulfide bond cross-links and prepared by a simple method
Int J Biol Macromol. 2023 Sep 6:126774. doi: 10.1016/j.ijbiomac.2023.126774. Online ahead of print.ABSTRACTChitosan was modified with thioctic acid and used to prepare soft films. As confirmed by FTIR and XPS measurements, a condensation reaction occurred between the amino groups in the chitosan and the carboxyl groups in the lipoic acid to form amide bonds in the modified chitosan. Films were then prepared by casting at ambient conditions, and the effects of the chemical modification on the physical-mechanical, antibacterial, and thermal properties of the films were investigated. The results showed that the tensile streng...
Source: International Journal of Biological Macromolecules - September 8, 2023 Category: Biochemistry Authors: Xinyue Wang Qiang Wang Ping Wang Man Zhou Bo Xu Ying Liu Yuanyuan Yu Source Type: research
Synthesis of novel naphthalene-chimonanthine scaffolds hybrids with potent antibacterial or antifungal activity
Nat Prod Res. 2023 Oct-Nov;37(19):3261-3266. doi: 10.1080/14786419.2022.2067851. Epub 2022 Apr 24.ABSTRACTIn this work, a total of 19 novel naphthalene hybrids with chimonanthine scaffolds were efficiently synthesised from indole-3-acetonitrile in good yields. The prepared compounds were evaluated for biological activity against Cryptococcus neoformans, Escherichia coli, Shigella spp, Candida albicans, Salmonella spp, and Staphylococcus aureus. The preliminary bioassays showed that most of the synthesised compounds exhibited significant antibacterial or antifungal activity. Notably, compound 8 showed potent activity agains...
Source: Natural Product Research - September 8, 2023 Category: Biochemistry Authors: Shaojun Zheng Wenbin Wu Qiaoju Jiang Chuansong Lin Yue Fang Huihui Dai Bing Tang Yi Tan Source Type: research
Antibacterial activity and mechanisms of α-terpineol against foodborne pathogenic bacteria
This study aimed to evaluate the antibacterial activities of α-terpineol against common foodborne pathogenic bacteria by agar well diffusion, broth microdilution, and colony counting assay. Propulsive research was conducted to reveal the antibacterial mechanisms, including morphology, infrared spectroscopy, membrane fluidity, membrane permeability, proton motive force, and oxidative phosphorylation. Results indicated that the antibacterial activity of α-terpineol decreased in the following order: Escherichia coli O157:H7, Salmonella typhimurium, Listeria monocytogenes, and Staphylococcus aureus. With an initial cell coun...
Source: Applied Microbiology and Biotechnology - September 8, 2023 Category: Microbiology Authors: Xiaoling Yang Shunan Zhao Yong Deng Weidong Xu Zonghan Wang Wenjun Wang Ruiling Lv Donghong Liu Source Type: research
Efficient production of α-ketoglutaric acid using an economical double-strain cultivation and catalysis system
Appl Microbiol Biotechnol. 2023 Sep 8. doi: 10.1007/s00253-023-12757-0. Online ahead of print.ABSTRACTThe whole-cell catalysis strategy of alpha-ketoglutaric acid (α-KG) production from L-glutamic acid (L-Glu) using recombinant Escherichia coli, in which L-glutamate oxidase (LGox) was over-expressed, has replaced the traditional chemical synthesis strategy. However, large amounts of toxic by-product, H2O2, should be eliminated through co-expressing catalase (Cat), thus severely increasing burden in cells. To efficiently and economically produce α-KG, here, the genes SpLGox (from Streptomyces platensis NTU3304) and SlCat ...
Source: Applied Microbiology and Biotechnology - September 8, 2023 Category: Microbiology Authors: Kun Liu Yan Liu Xiangfei Li Xiushan Zhang Zhenglian Xue Ming Zhao Source Type: research
Peripheral substitution effects on unnatural base pairs: A case of brominated TPT3 to enhance replication fidelity
Bioorg Chem. 2023 Aug 30;140:106827. doi: 10.1016/j.bioorg.2023.106827. Online ahead of print.ABSTRACTThe high fidelity poses a central role in developing unnatural base pairs (UBPs), which means the high pairing capacity of unnatural bases with their partners, and low mispairing with all the natural bases. Different strategies have been used to develop higher-fidelity UBPs, including optimizing hydrophobic interaction forces between UBPs. Variant substituent groups are allowed to fine tune the hydrophobic forces of different UBPs' candidates. However, the modifications on the skeleton of TPT3 base are rare and the replica...
Source: Bioorganic Chemistry - September 8, 2023 Category: Chemistry Authors: Bianbian Huo Chao Wang Xiaoqi Hu Honglei Wang Gongming Zhu Anlian Zhu Lingjun Li Source Type: research
