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Application of flow cytometry in transfusion medicine: The Sanjay Gandhi Post Graduate Institute of Medical Sciences, India experience
Asian J Transfus Sci. 2022 Jul-Dec;16(2):159-166. doi: 10.4103/ajts.ajts_61_22. Epub 2022 Sep 28.ABSTRACTThe application of flow cytometry (FC) is diverse and this powerful tool in used in multiple disciplines such as molecular biology, immunology, cancer biology, virology, and infectious disease screening. FC analyzes a single cell or a particle very rapidly as they flow past single or multiple lasers while suspended in buffered solution. FC has a great impact in the field of transfusion medicine (TM) due to its ability to analyze individual cell population and cell epitopes by sensitive, reproducible, and objective metho...
Source: Cancer Control - January 23, 2023 Category: Cancer & Oncology Authors: Rajendra Chaudhary Sudipta Sekhar Das Source Type: research

Making personalized, autologous cell therapies accessible: interview with Nabiha Saklayen and Marinna Madrid of  Cellino Biotech Inc
Regen Med. 2022 Mar 8. doi: 10.2217/rme-2022-0027. Online ahead of print.ABSTRACTNabiha Saklayen is CEO and Co-Founder of Cellino. Cellino's proprietary technology makes stem cell-derived therapies scalable for the first time. Nabiha was selected as a Pioneer in MIT Tech Review's 35 Innovators under 35 list for her patented inventions in cellular laser editing. She received her PhD in physics from Harvard University as a Howard Hughes Medical Institute (HHMI) International Fellow. She is also the inaugural Tory Burch Foundation Fellow in Genomics at the Innovative Genomics Institute led by Nobel laureate, Dr Jennifer Doudn...
Source: Regenerative Medicine - March 8, 2022 Category: Genetics & Stem Cells Authors: Nabiha Saklayen Marinna Madrid Source Type: research

Global Gene Expression Signatures in Response to Citrate-coated Silver Nanoparticles Exposure
Toxicology. 2021 Aug 14:152898. doi: 10.1016/j.tox.2021.152898. Online ahead of print.ABSTRACTSilver nanoparticles (AgNPs) are widely used in medical and commercial products for their unique antibacterial functions. However, the impact of AgNPs on human neural development is not well understood. To investigate the effect of AgNPs on human neural development, various doses of 20 nm citrate-coated AgNP (AgSC) were administered to human embryonic stem cell derived neural progenitors during the neuronal differentiation. Immunofluorescence staining with neuronal progenitor markers SOX2 (sex determining region Y-box 2) and Nesti...
Source: Toxicology - August 17, 2021 Category: Toxicology Authors: Hao Li Qingshun Q Li Yiling Hong Source Type: research

Myelodysplastic Syndrome-Associated SRSF2 Mutations Cause Splicing Changes by Altering Binding Motif Sequences
In this study, we characterized MDS-associated SRSF2 mutants (P95H, P95L, and P95R). We found that those mutants and wild-type SRSF2 proteins showed nuclear localization in HeLa cells. In vitro splicing reaction also revealed that mutant proteins associated with both precursor and spliced mRNAs, suggesting that the mutants directly participate in splicing. We established the human myeloid leukemia K562 cell lines that stably expressed myc-tagged wild-type or mutant SRSF2 proteins, and then performed RNA-sequence to analyze the splicing pattern of each cell line. The results revealed that both wild-type and mutants affected...
Source: Frontiers in Genetics - April 15, 2019 Category: Genetics & Stem Cells Source Type: research

Next-Generation Cancer Immunotherapy Targeting Glypican-3
This study was supported in part by the National Cancer Center Research and Development Fund (25-A-7 and 28-A-8); Health and Labor Science Research Grants for Clinical Research, Japan; and joint research funding from Takeda Pharmaceutical Co, Ltd.; Noile-Immune Biotech Inc.; Ono Pharmaceutical Co., Ltd.; BrightPath Biotherapeutics Co., Ltd.; and Sysmex Co., Ltd. This study was performed as part of a research program of the Project for Development of Innovative Research on Cancer Therapeutics (P-Direct), Ministry of Education, Culture, Sports, Science and Technology of Japan. Conflict of Interest Statement TN, TS, and TY ...
Source: Frontiers in Oncology - April 9, 2019 Category: Cancer & Oncology Source Type: research

Statistical Approaches for Estimating Sex-Specific Effects in Endocrine Disruptors Research
Conclusions: Investigators should consider sexual heterogeneity of confounder associations when choosing an analytic approach to estimate sex-specific effects of endocrine disruptors on health. In the presence of sex-dependent confounding, our augmented product term approach may be advantageous over stratification when there is prior knowledge available to fit reduced models or when investigators seek an automated test for effect measure modification. https://doi.org/10.1289/EHP334 Received: 08 April 2016 Revised: 29 November 2016 Accepted: 12 December 2016 Published: 23 June 2017 Address correspondence to J.P. Buckl...
Source: EHP Research - June 24, 2017 Category: Environmental Health Authors: Karla Gonzalez Tags: Research Source Type: research

Individual and Joint Effects of Early-Life Ambient PM2.5 Exposure and Maternal Prepregnancy Obesity on Childhood Overweight or Obesity
Conclusions: In the present study, we observed that early life exposure to PM2.5 may play an important role in the early life origins of COWO and may increase the risk of COWO in children of mothers who were overweight or obese before pregnancy beyond the risk that can be attributed to MPBMI alone. Our findings emphasize the clinical and public health policy relevance of early life PM2.5 exposure. https://doi.org/10.1289/EHP261 Received: 29 March 2016 Revised: 08 August 2016 Accepted: 23 August 2016 Published: 14 June 2017 Address correspondence to X. Wang, Center on the Early Life Origins of Disease, Department of P...
Source: EHP Research - June 14, 2017 Category: Environmental Health Authors: Daniil Lyalko Tags: Research Source Type: research

Neuromuscular disorders: genes, genetic counseling and therapeutic trials
Abstract Neuromuscular disorders (NMD) are a heterogeneous group of genetic conditions, with autosomal dominant, recessive, or X-linked inheritance. They are characterized by progressive muscle degeneration and weakness. Here, we are presenting our major contributions to the field during the past 30 years. We have mapped and identified several novel genes responsible for NMD. Genotype-phenotype correlations studies enhanced our comprehension on the effect of gene mutations on related proteins and their impact on clinical findings. The search for modifier factors allowed the identification of a novel "protective"; variant w...
Source: Genetics and Molecular Biology - August 30, 2016 Category: Genetics & Stem Cells Source Type: research

The NIEHS Superfund Research Program: 25 Years of Translational Research for Public Health
Conclusion: SRP must continue to address the legacy of hazardous waste in the United States, respond to new issues caused by rapid advances in technology, and train the next generation of leaders in environmental health science while recognizing that most of the world’s worst toxic hot spots are now located in low- and middle-income countries. Citation: Landrigan PJ, Wright RO, Cordero JF, Eaton DL, Goldstein BD, Hennig B, Maier RM, Ozonoff DM, Smith MT, Tukey RH. 2015. The NIEHS Superfund Research Program: 25 years of translational research for public health. Environ Health Perspect 123:909–918; http:/...
Source: EHP Research - September 30, 2015 Category: Environmental Health Authors: Sam Duvall Tags: Commentary October 2015 Source Type: research