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Specialty: Molecular Biology
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Total 744 results found since Jan 2013.

Isolation and Characterization of Single-Domain Antibodies from Immune Phage Display Libraries
We present and discuss comprehensive, step-by-step methods for isolating and characterization of antigen-specific VHHs. This includes protocols for expression, biotinylation, purification, and characterization of the isolated VHHs. To demonstrate the feasibility of the entire strategy, we present examples of VHHs previously isolated and characterized in our laboratory.PMID:37679618 | DOI:10.1007/978-1-0716-3381-6_7
Source: Mol Biol Cell - September 7, 2023 Category: Molecular Biology Authors: Martin A Rossotti Frederic Trempe Henk van Faassen Greg Hussack Mehdi Arbabi-Ghahroudi Source Type: research

Japanese Encephalitis virus infection in astrocytes modulate microglial function: Correlation with inflammation and oxidative stress
CONCLUSION: Astrocytic mediators like TNF-α, MCP-1 and IL-6 influence microglial response towards JEV infection by promoting inflammation and oxidative stress in them. As a result of increased microglial inflammation and secretion of ROS, viral replication is lessened in conditioned media treated and JEV infected microglial cells as compared to control cells with no conditioned media treatment but only JEV infection.PMID:37567102 | DOI:10.1016/j.cyto.2023.156328
Source: Cytokine - August 11, 2023 Category: Molecular Biology Authors: Stuti Mohapatra Triparna Chakraborty Anirban Basu Source Type: research

Micromorphological observation of HLE cells under knockdown of Fascin using LV-SEM
In this study, we attempted to reveal the relationship between Fascin and HCC using HLE, one of the human HCC cell lines. We performed the study with classical immunocytochemistry and recently developed techniques, such as wound-healing assay, spheroid cultivation, and low-vacuum scanning electron microscopy (LV-SEM). Non-Fascin-knockdown (FKD) cell spheroid had a regular spherical appearance with tight cell-cell connections, while FKD cell spheroid had an irregular shape with loose cell-cell connections. Cells of non-FKD spheroid presented fibrous protrusions on the cell surface, contrarily, cells of FKD spheroids showed ...
Source: Molecular Medicine - August 1, 2023 Category: Molecular Biology Authors: Yoshihiro Hayashi Yumiko Yamamoto Ichiro Murakami Source Type: research

Genetic variability of rice stripe virus after its pandemic in Jiangsu
CONCLUSIONS: Our results suggested that NSvc4 gene was susceptible to selection pressure, and the subtype Ib might be more adaptable for the interaction between RSV and hosts in the non-epidemic ecological conditions.PMID:37422539 | DOI:10.1007/s11033-023-08652-6
Source: Molecular Biology Reports - July 8, 2023 Category: Molecular Biology Authors: Chengye Lu Qian Miu Daoran Jin Aiguo Li Zhaobang Cheng Yijun Zhou Yunyue Wang Shuo Li Source Type: research

First report of the mitogenome of the invasive reef-building polychaete Ficopomatus enigmaticus (Annelida: Serpulidae) and a cryptic lineage from the Japanese Archipelago
CONCLUSIONS: The first mitochondrial genome of F. enigmaticus showed a unique gene order rearrangement, corroborating the remarkable diversity in the previously reported mitogenomes of other serpulid species. The presence of the two lineages of F. enigmaticus identified for the first time in Japan represents another case of cryptic invasion. The first 16S rRNA gene sequences of F. enigmaticus obtained in the present study can be used as reference sequences in future DNA metabarcoding studies.PMID:37407804 | DOI:10.1007/s11033-023-08647-3
Source: Molecular Biology Reports - July 5, 2023 Category: Molecular Biology Authors: Genki Kobayashi Hajime Itoh Nobuyoshi Nakajima Source Type: research

GHR-mutant pig derived from domestic pig and microminipig hybrid zygotes using CRISPR/Cas9 system
CONCLUSIONS: We have successfully demonstrated the generation of biallelic GHR-mutant small-stature pigs. Backcrossing of GHR-deficient pig with microminipig will establish the smallest pig strain which can contribute significantly to the field of biomedical research.PMID:37101010 | DOI:10.1007/s11033-023-08388-3
Source: Molecular Biology Reports - April 26, 2023 Category: Molecular Biology Authors: Fuminori Tanihara Maki Hirata Zhao Namula Manita Wittayarat Lanh Thi Kim Do Qingyi Lin Koki Takebayashi Hiromasa Hara Megumi Nagahara Takeshige Otoi Source Type: research

Complete chloroplast genome and phylogenetic analysis of Anemone shikokiana
CONCLUSIONS: The results filled the gap of cp genome sequence information of A. shikokiana, laying the foundation to explore the evolutionary relationships of A. shikokiana in future studies. It provided a valuable genetic resource for the molecular identification and phylogenetic study of Anemone.PMID:36542234 | DOI:10.1007/s11033-022-08204-4
Source: Molecular Biology Reports - December 21, 2022 Category: Molecular Biology Authors: Kang An Chunxia Zhou Lei Feng Qing Zhang Yujuan Pang Lixia Li Fuhua Bian Source Type: research

Novel nomogram developed for determining suitability of metastatic castration-resistant prostate cancer patients to receive maximum benefit from radium-223 dichloride treatment-Japanese Ra-223 Therapy in Prostate Cancer using Bone Scan Index (J-RAP-BSI) Trial
Eur J Nucl Med Mol Imaging. 2022 Dec 21. doi: 10.1007/s00259-022-06082-3. Online ahead of print.NO ABSTRACTPMID:36539508 | DOI:10.1007/s00259-022-06082-3
Source: Molecular Medicine - December 20, 2022 Category: Molecular Biology Authors: Kazuhiro Kitajima Masataka Igeta Junpei Kuyama Takashi Kawahara Tsuyoshi Suga Tomoaki Otani Shigeyasu Sugawara Yumiko Kono Yukihisa Tamaki Ayumi Seko-Nitta Yoshinobu Ishiwata Kimiteru Ito Akira Toriihara Shiro Watanabe Makoto Hosono Hideaki Miyake Shingo Source Type: research

The emergence of human metapneumovirus G gene duplication in hospitalized patients with respiratory tract infection, India, 2016-2018
CONCLUSION: In the current study, we report that A2c is the sub-lineage in India from April 2016 to August 2018. This study is the first retrospective study reporting the circulation of the A2c sub-lineage among adults in India with 180- and 111-nucleotide duplications in the G gene of human metapneumovirus.PMID:36399244 | DOI:10.1007/s11033-022-08092-8
Source: Molecular Biology Reports - November 18, 2022 Category: Molecular Biology Authors: Preetiparna Parida Sudheesh N Sanjay E R Anitha Jagadesh Srilatha Marate Arunkumar Govindakaranavar Source Type: research

The journey of hull-fouling mobile invaders: basibionts and boldness mediate dislodgement risk during transit
Biofouling. 2022 Nov 1:1-15. doi: 10.1080/08927014.2022.2138754. Online ahead of print.ABSTRACTVessel hull-fouling is responsible for most bioinvasion events in the marine environment, yet it lacks regulation in most countries. Although experts advocate a preventative approach, research efforts on pre-arrival processes are limited. The performance of mobile epifauna during vessel transport was evaluated via laboratory simulations, using the well-known invasive Japanese skeleton shrimp (Caprella mutica), and its native congener C. laeviuscula as case study. The invader did not possess any advantage in terms of inherent resi...
Source: Biofouling - November 1, 2022 Category: Molecular Biology Authors: Gemma Mart ínez-Laiz Colin D MacLeod Amelia V Hesketh Cassandra A Konecny Macarena Ros Jos é M Guerra-García Christopher D G Harley Source Type: research