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Specialty: Reproduction Medicine

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Total 316 results found since Jan 2013.

Up-regulation of AREG, EGFR and HER2 contributes to increased VEGF expression in granulosa cells of patients with OHSS †.
This study demonstrated that granulosa cell-secreted AREG plays an important role in the development of OHSS, suggesting that the EGFR/HER2-mediated signaling could be a novel drug target for the prevention and treatment of OHSS. PMID: 31167229 [PubMed - as supplied by publisher]
Source: Reproductive Biology - June 4, 2019 Category: Reproduction Medicine Authors: Fang L, Yu Y, Li Y, Wang S, He J, Zhang R, Sun YP Tags: Biol Reprod Source Type: research

SMAD-dependent signaling mediates BMP6-induced stimulation of connective tissue growth factor in luteinized human granulosa cells.
In this study, we demonstrated that BMP6 treatment significantly increased the expression of CTGF in both primary and immortalized human granulosa cells. Using both pharmacological inhibitors and siRNA-mediated knockdown approaches, we showed that ALK2 and ALK3 type I receptors are required for BMP6-induced cellular activities. Furthermore, this effect is most likely mediated by a SMAD-dependent pathway. Our studies provide novel insight into the molecular mechanisms by which an intraovarian growth factor affects the production of another factor via a paracrine effect in human granulosa cells. PMID: 31210269 [PubMed -...
Source: Biology of Reproduction - June 17, 2019 Category: Reproduction Medicine Authors: Liu S, Chang HM, Yi Y, Yao YQ, Leung PCK Tags: Biol Reprod Source Type: research

Endogenous retrovirus-encoded Syncytin-2 contributes to exosome-mediated immunosuppression of T cells.
Abstract Modulation of the activation status of immune cell populations during pregnancy depends on placental villous cytotrophoblast (VCT) cells and the syncytiotrophoblast (STB) layer. Failure in the establishment of this immunoregulatory function leads to pregnancy complications. Our laboratory has been studying Syncytin-2 (Syn-2), an endogenous retroviral protein expressed in placenta and on the surface of placental exosomes. This protein plays an important role in STB formation through its fusogenic properties, but also possesses an active immunosuppressive domain (ISD). Considering that Syn-2 expression is i...
Source: Biology of Reproduction - July 17, 2019 Category: Reproduction Medicine Authors: Lokossou AG, Toudic C, Nguyen PT, Elisseeff X, Vargas A, Rassart É, Lafond J, Leduc L, Bourgault S, Gilbert C, Scorza T, Tolosa J, Barbeau B Tags: Biol Reprod Source Type: research

Yes-associated protein (YAP1) is required for proliferation and function of bovine granulosa cells in vitro$.
Abstract Yes-associated protein (YAP1) is a major component of the Hippo signaling pathway. Although the exact extracellular signals that control the Hippo pathway are currently unknown, increasing evidence supports a critical role for the Hippo pathway in embryonic development, regulation of organ size, and carcinogenesis. Granulosa cells within the ovarian follicle proliferate and produce steroids and growth factors, which facilitate growth of follicle and maturation of the oocyte. We hypothesize that YAP1 plays a role in proliferation and estrogen secretion of granulosa cells. In the current study, we examined ...
Source: Biology of Reproduction - July 26, 2019 Category: Reproduction Medicine Authors: Plewes MR, Hou X, Zhang P, Liang A, Hua G, Wood JR, Cupp AS, Lv X, Wang C, Davis JS Tags: Biol Reprod Source Type: research

Synergistic inhibition of csal1 and csal3 in granulosa cell proliferation and steroidogenesis of hen ovarian prehierarchical development.
This study aimed to explore the potential role and mechanism of csal1 and csal3 in granulosa cells (GCs) proliferation, differentiation, and follicle selection within the PFs of hen ovary. Our data demonstrated that the csal1 and csal3 transcriptions were highly expressed in GCs of the various-sized PFs and their proteins were mainly localized in the cytoplasm of granulosa cells and oocytes of the examined PFs as well as in the ovarian stroma and epithelium. It initially revealed that both csal1 and csal3 may be involved in chicken PF development via a translocation mechanism. Furthermore, our results showed an abundance o...
Source: Biology of Reproduction - July 26, 2019 Category: Reproduction Medicine Authors: Zhu H, Qin N, Xu X, Sun X, Chen X, Zhao J, Xu R, Mishra B Tags: Biol Reprod Source Type: research

Fibrinogen alpha chain is upregulated and affects the pathogenesis of endometriosis
ConclusionsHigh expression of FGA in EM was closely related to disease severity and affected cell adhesion, migration, and invasion, which might play an important role in the pathogenesis of EM.
Source: Reproductive BioMedicine Online - August 21, 2019 Category: Reproduction Medicine Source Type: research

The distribution and expression of vascular endothelial growth factor A (VEGFA) during follicular development and atresia in the pig.
This study investigated the distribution and expression of VEGFA during porcine follicular development and atresia. Pig ovaries were obtained, individual medium-sized (3-5mm in diameter) antral follicles were separated and classified into healthy, early atretic or progressively atretic groups. Immunobiology and quantitative techniques were used to investigate the varied follicular distribution of VEGFA at both the morphological and molecular level. The results indicated that VEGFA protein expression peaked in tertiary follicles, mostly distributed in the thecal and inner granulosa layers, during follicular development whil...
Source: Reproduction, Fertility, and Development - September 23, 2019 Category: Reproduction Medicine Authors: Gao X, Zhang J, Pan Z, Li Q, Liu H Tags: Reprod Fertil Dev Source Type: research

Role of vaspin in porcine ovary: effect on signaling pathways and steroid synthesis via GRP78 receptor and protein kinase A.
Abstract Vaspin, visceral adipose tissue-derived serine protease inhibitor, is involved in the development of obesity, insulin resistance, inflammation and energy metabolism. Our previous study showed vaspin expression and its regulation in the ovary; however, the role of this adipokine in ovarian cells has never been studied. Here, we studied in vitro the effect of vaspin on various kinase signaling pathways: mitogen-activated kinase (MAP3/1), serine/threonine kinase (AKT), signal transducer and activator of transcription 3 (STAT3) protein kinase AMP (PRKAA1), protein kinase A (PKA) and on expression of nuclear f...
Source: Biology of Reproduction - March 8, 2020 Category: Reproduction Medicine Authors: Kurowska P, Mlyczyńska E, Dawid M, Dupont J, Rak A Tags: Biol Reprod Source Type: research

hCG improves luteal function and promotes progesterone output through the activation of JNK pathway in the luteal granulosa cells of the stimulated IVF cycles.
Abstract Human chorionic gonadotropin (hCG) is a luteotropic hormone that promotes the survival and steroidogenic activity of corpus luteum by acting through LH receptors expressed on luteinized theca and granulosa cells. Therefore, it is used to support luteal phase in IVF cycles in order to improve clinical pregnancy rates and prevent miscarriage. However, the molecular mechanism underlying this action of hCG is not well-characterized. To address this question we designed an in vitro translational research study on the luteal granulosa cells (GCs) obtained from 58 IVF patients. hCG treatment at different concent...
Source: Reproductive Biology - March 11, 2020 Category: Reproduction Medicine Authors: Bildik G, Akin N, Esmaeilian Y, Hela F, Yakin K, Onder T, Urman B, Oktem O Tags: Biol Reprod Source Type: research

Interleukin-13 receptor subunit alpha-2 is a target of progesterone receptor and steroid receptor coactivator-1 in the mouse uterus †.
In this study, we employed PGR knockout mice (PRKO) and steroid receptor coactivator-1 knockout mice (SRC-1-/-) to profile the expression of Il13ra2 in the murine endometrium and determine the role of these transcriptional regulators in the hormone-responsiveness of Il13ra2 expression. Furthermore, we utilized a well-established decidualization-inducing steroidogenic cocktail and a siRNA-based knockdown of IL13RA2 to determine the importance of IL13RA2 in the decidualization of primary human endometrial stromal cells. Our findings demonstrate that Il13ra2 is expressed in the sub-epithelial stroma of the murine endometrium ...
Source: Biology of Reproduction - June 18, 2020 Category: Reproduction Medicine Authors: Marquardt RM, Lee K, Kim TH, Lee B, DeMayo FJ, Jeong JW Tags: Biol Reprod Source Type: research

Characterization of the role for cadherin 6 in the regulation of human endometrial receptivity.
CONCLUSION: Together, our data revealed that CDH6 was dysregulated in the endometrium from women with infertility and altered Ishikawa cell adhesive capacity. Our study supports a role for CDH6 in regulating endometrial adhesion and implantation. PMID: 32600462 [PubMed - in process]
Source: Reproductive Biology - June 28, 2020 Category: Reproduction Medicine Authors: Zhou W, Santos L, Dimitriadis E Tags: Reprod Biol Endocrinol Source Type: research

Embryonic MTHFR contributes to blastocyst development
ConclusionThe results indicate that embryonic MTHFR is indispensable for normal blastocyst development. The findings provide insight into the debatable roles of MTHFR in fertility and may be applicable for the improvement of care for early embryos via modulation of surrounding folate-related nutritional conditions in vitro and/or in utero, depending on the parental and embryonicMTHFR genotype.
Source: Journal of Assisted Reproduction and Genetics - August 6, 2020 Category: Reproduction Medicine Source Type: research

The role of ORC4 in enucleation of Murine Erythroleukemia (MEL) cells is similar to that in oocyte polar body extrusion.
Abstract The Origin Replication Complex subunit 4 (ORC4) is one in six subunits of the Origin Replication Complexes (ORCs) which is essential for initiating licensing at DNA replication origins and recruiting adaptor molecules necessary for various cellular processes. Previously, we reported that ORC4 also plays a vital role in polar body extrusion (PBE) during oogenesis in which half the chromosomes are extruded from the oocyte. We hypothesized that ORC4 might play a broader role in chromatin elimination. We tested its role in enucleation during the development of erythrocytes. Murine erythroleukemia (MEL) cells ...
Source: Systems Biology in Reproductive Medicine - September 23, 2020 Category: Reproduction Medicine Authors: Nguyen H, Ung A, Ward WS Tags: Syst Biol Reprod Med Source Type: research

Sirtuin-1 Inhibits Endothelin-2 Expression in Human Granulosa-Lutein Cells via HIF1A and Epigenetic Modifications †.
Sirtuin-1 Inhibits Endothelin-2 Expression in Human Granulosa-Lutein Cells via HIF1A and Epigenetic Modifications†. Biol Reprod. 2020 Oct 28;: Authors: Szymanska M, Manthe S, Shrestha K, Girsh E, Harlev A, Kisliouk T, Meidan R Abstract Endothelin-2 (EDN2) expression in granulosa cells was previously shown to be highly dependent on the hypoxic mediator, HIF1A. Here we investigated whether sirtuin-1 (SIRT1), by deacetylating HIF1A and class III histones, modulates EDN2 in human granulosa-lutein cells (hGLCs). We found that HIF1A was markedly suppressed in the presence of resveratrol or a specific SIRT...
Source: Biology of Reproduction - October 28, 2020 Category: Reproduction Medicine Authors: Szymanska M, Manthe S, Shrestha K, Girsh E, Harlev A, Kisliouk T, Meidan R Tags: Biol Reprod Source Type: research

Osteoprotegerin interacts with syndecan-1 to promote human endometrial stromal decidualization by decreasing Akt phosphorylation
In this study, based on a number of approaches, it was demonstrated that OPG mediated the repression of Akt that occurs during human stromal cell decidualization, however, the molecular link between OPG and Akt signaling was not determined, and still requires further exploration.WIDER IMPLICATIONS OF THE FINDINGSOPG is required for decidualization, and a decrease in OPG levels is associated with RPL. These findings provide a new candidate molecule for the diagnosis and potential treatment of RPL.STUDY FUNDING/COMPETING INTEREST(S)This work was supported in part by the National Natural Science Foundation of China U1605223 (...
Source: Human Reproduction - October 13, 2020 Category: Reproduction Medicine Source Type: research