• Filter By:
• Specialty
• Source
• Condition
• Cancer
• Infectious Disease
• Drug
• Training
• Management
• Procedure
• Therapy
• Vaccine
• Nutrition
• Country

Spray drying siRNA-lipid nanoparticles for dry powder pulmonary delivery
This study verifies the successful spray drying procedure of LNP-siRNA systems maintaining their integrity and mediating strong gene silencing efficiency on mRNA and protein levels both in vitro and ex vivo. The successful spray drying procedure of LNP-siRNA formulations in 5% lactose solution creates a novel siRNA-based therapy option to target respiratory diseases such as lung cancer, asthma, COPD, cystic fibrosis and viral infections.PMID:36126785 | DOI:10.1016/j.jconrel.2022.09.021
Source: Cancer Control - September 20, 2022 Category: Cancer & Oncology Authors: Christoph M Zimmermann Domizia Baldassi Karen Chan Nathan B P Adams Alina Neumann Diana Leidy Porras-Gonzalez Xin Wei Nikolaus Kneidinger Mircea Gabriel Stoleriu Gerald Burgstaller Dominik Witzigmann Paola Luciani Olivia M Merkel Source Type: research

Effect of pre ‑freezing and saccharide types in freeze‑drying of siRNA lipoplexes on gene‑silencing effects in the cells by reverse transfection.
Effect of pre‑freezing and saccharide types in freeze‑drying of siRNA lipoplexes on gene‑silencing effects in the cells by reverse transfection. Mol Med Rep. 2020 Aug 05;: Authors: Tang M, Hu S, Hattori Y Abstract Our previous study reported that reverse (Rev)‑transfection with small interfering RNA (siRNA)/cationic liposome complexes (siRNA lipoplexes) freeze‑dried in trehalose or sucrose solution resulted in high gene‑silencing activity in cells. The current study investigated whether pre‑freezing or saccharide types present during the freeze‑drying of siRNA lipoplexes affected geneâ€...
Source: Molecular Medicine Reports - September 20, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Growth factor receptor bound protein-7 regulates proliferation, cell cycle, and mitochondrial apoptosis of thyroid cancer cells via MAPK/ERK signaling.
Abstract It is of great significance to explore the molecular mechanism of thyroid cancer (TC) pathogenesis for its improvement and therapy. Growth factor receptor bound protein-7 (GRB7) has been regarded as an important regulatory gene in the developments of various malignant tumors. Our study aimed to illustrate the role of GRB7 in the TC pathology mechanism. Firstly, GRB7 was found to be significantly upregulated in 49 cases of TC tissues and 5 TC cell lines by using real-time quantitative polymerase chain reaction (RT-qPCR) and western blotting. Silencing GRB7 with siRNA dramatically inhibited proliferation an...
Source: Molecular and Cellular Biochemistry - June 22, 2020 Category: Biochemistry Authors: Tang H, Yang P, Yang X, Peng S, Hu X, Bao G Tags: Mol Cell Biochem Source Type: research

GRP78 regulates milk biosynthesis and the proliferation of bovinemammaryepithelial cells through the mTOR signaling pathway.
Conclusions: These data reveal that GRP78 is an important regulator of milk biosynthesis and the proliferation of BMECs through the mTOR signaling pathway. PMID: 31660059 [PubMed - in process]
Source: Cellular and Molecular Biology Letters - October 31, 2019 Category: Biochemistry Authors: Liu Y, Wang X, Zhen Z, Yu Y, Qiu Y, Xiang W Tags: Cell Mol Biol Lett Source Type: research

Transcription factor E2F4 is a positive regulator of milk biosynthesis and proliferation of bovine mammary epithelial cells.
This article is protected by copyright. All rights reserved. PMID: 31475773 [PubMed - as supplied by publisher]
Source: Cell Biology International - September 1, 2019 Category: Cytology Authors: Zhen Z, Zhang M, Yuan X, Li M Tags: Cell Biol Int Source Type: research

Preparation and Cell Activities of Lactosylated Curdlan-triornithine Nanoparticles for Enhanced DNA/siRNA Delivery in Hepatoma Cells
Publication date: Available online 27 August 2019Source: Carbohydrate PolymersAuthor(s): Xia Wang, Yuxuan Qi, Lixia Liu, Tsogzolmaa Ganbold, Huricha Baigude, Jingfen HanAbstractA long-anticipated cancer therapy would deliver the right type of therapeutic agents to the target in control with minimal systemic toxicity. The purpose of this study was to prepare lactosylated curdlan-triornithine nanocarriers (CTOLs), and target deliver gene to hepatoma cells. Structures and biophysical properties had been elucidated with physical and chemical methods. The results revealed that those functionalized polymers can completely conden...
Source: Carbohydrate Polymers - August 28, 2019 Category: Biomedical Science Source Type: research

The system with zwitterionic lactose-based surfactant for complexation and delivery of small interfering ribonucleic acid—A structural and spectroscopic study
This article presents results of a study on the structural effects associated with siRNA complexation by a surfactant comprising a lactose group (N-(3-propanesulfone)-N-dodecyl-amino-beta-D-lactose hydrochloride, LA12). The double stranded siRNA oligomer (21 base pairs) used in this study is responsible for silencing a gene that can be important in the therapy of myotonic dystrophy type 1. The obtained siRNA/LA12 lipoplexes were studied using the methods of small angle scattering of synchrotron radiation, circular dichroism spectroscopy, Fourier transform infrared spectroscopy, and electrophoretic mobility tests. ...
Source: Applied Physics Letters - May 24, 2016 Category: Physics Authors: Michalina Skupin, Krzysztof Sobczak, Ryszard Zieliński and Maciej Kozak Source Type: research