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Total 442 results found since Jan 2013.

Kisspeptin-54 attenuates oxidative stress and neuronal apoptosis in early brain injury after subarachnoid hemorrhage in rats via GPR54/ARRB2/AKT/GSK3 β signaling pathway
In conclusion, our results suggested that administration of KP54 attenuated oxidative stress, neuronal apoptosis and neurobehavioral impairments through GPR54/ARRB2/AKT/GSK3β signaling pathway after SAH in rat. Thus, KP54 may provide an effective treatment strategy for SAH patients.PMID:33989759 | DOI:10.1016/j.freeradbiomed.2021.05.012
Source: Free Radical Biology and Medicine - May 14, 2021 Category: Biology Authors: Yi Huang Yong Guo Lei Huang Yuanjian Fang Dujuan Li Rui Liu Qin Lu Reng Ren Lihui Tang Lifei Lian Yongmei Hu Jiping Tang Gao Chen John H Zhang Source Type: research

Sodium fluoride activates the extrinsic apoptosis via regulating NOX4/ROS-mediated p53/DR5 signaling pathway in lung cells both in vitro and in vivo
In this study, we report that death receptor 5 (DR5), a major component of the extrinsic apoptotic pathway, is markedly induced upon NaF stimulation. Enhanced DR5 induction was necessary for the apoptotic effects of NaF, inasmuch as transfected BEAS-2B cells with DR5 siRNA attenuated NaF-induced caspase-8 activation in lung cells. Mechanism investigation indicated that the induction of DR5, following NaF exposure, was mediated by tumor protein 53 (p53)-dependent transcriptional activation. Notably, we demonstrated that NaF could induce a significant increase in intracellular reactive oxygen species (ROS) level derived from...
Source: Free Radical Biology and Medicine - April 15, 2021 Category: Biology Authors: Chao Song Dongmei Shi Kaiwen Chang Xianghui Li Qing Dong Xia Ma Xuefei Wang Zhenhuan Guo Yonglu Liu Jundong Wang Source Type: research

Dioscin ameliorates methotrexate-induced liver and kidney damages via adjusting miRNA-145-5p-mediated oxidative stress
Free Radic Biol Med. 2021 Apr 6:S0891-5849(21)00195-7. doi: 10.1016/j.freeradbiomed.2021.03.035. Online ahead of print.ABSTRACTDioscin, one natural product, has various pharmacological actions. However, its effects on methotrexate (MTX)-induced hepatorenal damages still remain unknown. In the present study, the data manifested that dioscin restored the viabilities of L-02 and NRK-52E cells, reduced ALT, AST, Cr, BUN levels, and ameliorated histopathological changes of liver and kidney. Besides, dioscin decreased ROS levels in cells, and adjusted SOD, MDA, GSH and GSH-Px levels in rats. Dioscin reduced the expression levels...
Source: Free Radical Biology and Medicine - April 9, 2021 Category: Biology Authors: Y Li M Gao L-H Yin L-N Xu Y Qi Pengyuan Sun J-Y Peng Source Type: research

C-C Motif Ligand 8 promotes atherosclerosis via NADPH Oxidase 2/Reactive Oxygen Species-induced endothelial permeability Increase
This study might contribute to a deeper understanding of the connection between chemokines and atherosclerosis.PMID:33741452 | DOI:10.1016/j.freeradbiomed.2021.02.022
Source: Free Radical Biology and Medicine - March 20, 2021 Category: Biology Authors: Song Xue Hanfei Tang Gefei Zhao Chao Fang Yang Shen Dong Yan Ye Yuan Weiguo Fu Zhenyu Shi Xiao Tang Daqiao Guo Source Type: research

LRRC8A contributes to angiotensin II-induced cardiac hypertrophy by interacting with NADPH oxidases via the C-terminal leucine-rich repeat domain.
Abstract Cardiac hypertrophy, an important cause of heart failure, is characterized by an increase in heart weight, the ventricular wall, and cardiomyocyte volume. The volume regulatory anion channel (VRAC) is an important regulator of cell volume. However, its role in cardiac hypertrophy remains unclear. The purpose of this study was to investigate the effect of leucine-rich repeat-containing 8A (LRRC8A), an essential component of the VRAC, on angiotensin II (AngII)-induced cardiac hypertrophy. Our results showed that LRRC8A expression, NADPH oxidase activity, and reactive oxygen species (ROS) production were inc...
Source: Free Radical Biology and Medicine - January 27, 2021 Category: Biology Authors: Huo C, Liu Y, Li X, Xu R, Jia X, Hou L, Wang X Tags: Free Radic Biol Med Source Type: research

NQO1 mediates the anti-inflammatory effects of nootkatone in lipopolysaccharide-induced neuroinflammation by modulating the AMPK signaling pathway.
Abstract Neuroinflammation and oxidative stress play key roles in the progression of neurodegenerative diseases. Thus, the use of potent anti-inflammatory/antioxidant agents has been suggested as a promising therapeutic strategy for neurodegenerative diseases. In the present study, we investigated the anti-inflammatory and antioxidant effects of nootkatone (NKT), a sesquiterpenoid compound isolated from grapefruit, in in vitro and in vivo models of neuroinflammation. In lipopolysaccharide (LPS)-stimulated BV2 microglial cells, NKT inhibited the expression of iNOS, COX-2, and pro-inflammatory cytokines, and increas...
Source: Free Radical Biology and Medicine - January 15, 2021 Category: Biology Authors: Park JE, Park JS, Leem YH, Kim DY, Kim HS Tags: Free Radic Biol Med Source Type: research

Up-regulation of thioredoxin system by puerarin inhibits lipid uptake in macrophages.
In this study, we investigated the effects of puerarin on lipid uptake and explored the underlying molecular regulation. We found puerarin up-regulated thioredoxin-1 (Trx1) and Trx reductase-1 (TrxR1) expression; it increased TrxR1 activity, cellular thiols contents and decreased oxidized form of Trx1, thus inhibiting cellular ROS generation. Confocal microscope and flow cytometry analysis showed fluorescence labeled Dil-oxLDL uptake was dramatically inhibited by puerarin in RAW264.7 cells as well as in primary bone marrow derived macrophages and peritoneal macrophages. The effects were reversed when Trx1 was inhibited by ...
Source: Free Radical Biology and Medicine - November 23, 2020 Category: Biology Authors: Li W, Xu X, Dong D, Lei T, Ou H Tags: Free Radic Biol Med Source Type: research

Role of BMAL1 and CLOCK in regulating the secretion of melatonin in chick retina under monochromatic green light.
Abstract As the circadian pacemaker of birds, the retina possesses the ability to receive light information, generate circadian oscillation, and secrete melatonin. Previous studies have confirmed that monochromatic green light can accelerate the circadian rhythmic expression of clock genes in the chick retina, thereby increasing cAanat mRNA level and melatonin secretion. However, as the core components of the transcriptional-translational negative feedback loop, the role that cBmal1 and cClock plays in the regulation of the retinal molecular clock system and melatonin secretion under monochromatic green light is u...
Source: Chronobiology International - October 28, 2020 Category: Biology Authors: Bian J, Wang Z, Dong Y, Cao J, Chen Y Tags: Chronobiol Int Source Type: research

Angelica dahurica promoted angiogenesis and accelerated wound healing in db/db mice via the HIF-1 α/PDGF-β signaling pathway.
In this study, we found that Angelica dahurica (A. dahurica) stimulated angiogenesis and benefited wound healing in genetic mouse models of diabetes. In HUVECs, A. dahurica promoted cell proliferation and tube formation, which was accompanied by increased nuclear translocation of HIF-1α under hypoxic conditions and led to elevated PDGF-β protein expression. A. dahurica activated the PI3K/AKT signaling pathway in human umbilical vein endothelial cells (HUVECs), which was abrogated by the PI3K inhibitor LY294002. Furthermore, the cellular expression of PDGF-β decreased significantly after treatment with a HIF-1α-siRNA, a...
Source: Free Radical Biology and Medicine - August 23, 2020 Category: Biology Authors: Guo J, Hu Z, Yan F, Lei S, Li T, Li X, Xu C, Sun B, Pan C, Chen L Tags: Free Radic Biol Med Source Type: research

Down regulation of glutathione and glutamate cysteine ligase in the inflammatory response of macrophages.
In conclusion, these data suggest that GCLC down-regulation in the inflammatory response of macrophages is mediated through both increased mRNA decay and caspase-5-mediated GCLC protein degradation, and γ-GC is an efficient agent to restore GSH and regulate the inflammatory response. PMID: 32682927 [PubMed - as supplied by publisher]
Source: Free Radical Biology and Medicine - July 15, 2020 Category: Biology Authors: Zhang H, Zhang SJ, Lyn N, Florentino A, Li A, Davies KJA, Forman HJ Tags: Free Radic Biol Med Source Type: research

Melatonin induces mitochondrial apoptosis in osteoblasts by regulating the STIM1/cytosolic calcium elevation/ERK pathway
This study aimed to determine the mechanism by which melatonin inhibits the proliferation of osteoblasts.Main methodsCell viability experiment, DNA fragment detection and alkaline phosphatase (ALP) activity assays were performed to determine the effects of melatonin on the proliferation, apoptosis and differentiation of osteoblasts. We used immunofluorescence to detect the expression of STIM1 in melatonin-treated osteoblasts. STIM1 interference was achieved using a specific siRNA, and a TRPC inhibitor was used to block the influx of Ca2+. The mRNA expression was determined by RT-qPCR, and protein levels were measured by We...
Source: Life Sciences - February 21, 2020 Category: Biology Source Type: research

Decreasing Arl4c expression by inhibition of AKT signal in human lung adenocarcinoma cells
ConclusionHere, we highlighted the AKT pathway as an important regulatory pathway for Arl4c expression in lung cancer cells and identified HCPT as a promising drug for lung adenocarcinoma treatment that functioned by targeting Arl4c expression.
Source: Life Sciences - February 13, 2020 Category: Biology Source Type: research