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Xenopus Resources: Transgenic, Inbred and Mutant Animals, Training Opportunities, and Web-Based Support
This reporter group also includes lines driven by ubiquitous promoters like CMV and human ubiquitin C. These are particularly useful for cut-and-paste, transplantation-based experiments to label and fate map regions of host embryos.
The second group of reporter expression lines includes transgenics marking subcellular organelles (Supplementary Table S1B). These are highly useful for the study of molecular processes involved in cell function and are especially effective when utilized in the context of Xenopus egg extracts, the only cell-free system that permits full investigation of all DNA transactions related to cell cyc...
Source: Frontiers in Physiology - April 24, 2019 Category: Physiology Source Type: research
Techniques for Studying Decoding of Single Cell Dynamics
Stevan Jeknić1,2†, Takamasa Kudo2,3† and Markus W. Covert1,2,3*
1Department of Bioengineering, Stanford University, Stanford, CA, United States
2Allen Discovery Center for Systems Modeling of Infection, Stanford, CA, United States
3Department of Chemical and Systems Biology, Stanford University, Stanford, CA, United States
Cells must be able to interpret signals they encounter and reliably generate an appropriate response. It has long been known that the dynamics of transcription factor and kinase activation can play a crucial role in selecting an individual cell's response. The ...
Source: Frontiers in Immunology - April 10, 2019 Category: Allergy & Immunology Source Type: research
The Functional and Molecular Effects of Doxycycline Treatment on Borrelia burgdorferi Phenotype
In this study, we aimed to identify gene expression patterns associated with the survival and re-growth of B. burgdorferi in the antibiotic environment. While a similar study in which RNASeq was applied to antibiotic treatment of B. burgdorferi was conducted (Feng et al., 2015), several important distinctions between that study and ours should be made. In that report by Feng et al, in vitro-cultured Bb were treated with either doxycycline or amoxicillin (50 μg/mL) for 6 days and then subjected to RNASeq, with comparison to the untreated control. Genes that were up-regulated by 2-fold or more were ascribed significan...
Source: Frontiers in Microbiology - April 17, 2019 Category: Microbiology Source Type: research
Antibiotics in mariculture organisms of different growth stages: Tissue-specific bioaccumulation and influencing factors
Environ Pollut. 2021 Jul 7;288:117715. doi: 10.1016/j.envpol.2021.117715. Online ahead of print.ABSTRACTMaricultured organisms are chronically exposed to water containing antibiotics but the bioaccumulative behavior of antibiotics in exposed organisms at different growth stages has received little attention. Here, we investigated the concentrations and tissue-specific bioaccumulation characteristics of 19 antibiotics during three growth stages (youth stage, growth stage, and adult stage) of various organisms (Scophthalmus maximus, Penaeus vannamei, Penaeus japonicus, and Apostichopus japonicus) cultivated in typical marine...
Source: Environmental Pollution - July 13, 2021 Category: Environmental Health Authors: Xuanrui Zhang Jiachao Zhang Qianfan Han Xiaoli Wang Shuguang Wang Xianzheng Yuan Baiyu Zhang Shan Zhao Source Type: research
Antibiotics and resistant genes in the gut of Chinese nine kinds of freshwater or marine fish
In this study, nine kinds of Chinese freshwater and marine fish were acquired in a city of northern China to test the amount of antibiotics and ARGs residues in their intestinal contents. The results showed that 4 kinds of antibiotics were detected from the intestinal contents, including Doxycycline (DOX), Tetracycline (TC), Sulfamethoxazole (SMX) and Roxithromycin (ROX), and the antibiotics with the largest detected amount was ROX in Sardinops sagax (2.83 μg kg-1). Ten kinds of ARGs were detected from the intestinal contents, including strA, strB, ermB, blaTEM, oxa-30, qnrB, qnrD, sul1, sul2 and tetB, as well as one type...
Source: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes - March 16, 2022 Category: Environmental Health Authors: Wenpeng Li Zhiguang Niu Xiaohan Zhang Kai Zhang Susu Luo Source Type: research
Detection of pathogenic Vibrio species and antibiogram activity in Asian Seabass (Lates calcarifer) in Tumpat, Kelantan
This study was conducted to investigate the prevalence of Vibrio spp. isolated from seabass (Lates calcarifer) in Sri Tujuh Lagoon, Tumpat, Kelantan. Then, to determine the antibiotic resistance in Vibrio isolates. Polymerase chain reaction (PCR) was used to detect Vibrio species using specific primer VR169 and VR744 with estimation base pair size band, 597 bp and further identified by sequencing. On the other hand, antibiotic susceptibility tests were continued by using 13 types of antibiotics; kanamycin (K30), chloramphenicol (C30), neomycin (N10), ampicillin (AMP10), nitrofurantoin (F300), tetracycline (TE30), streptomy...
Source: Tropical Biomedicine - January 5, 2023 Category: Tropical Medicine Authors: A M Z Auzureen M S Michael M Mohamed T L Peng F Fauzi N F A Mohamad N S Ahmad C W Z C W Salma R H Hamdan Source Type: research
