The Functional and Molecular Effects of Doxycycline Treatment on Borrelia burgdorferi Phenotype

The Functional and Molecular Effects of Doxycycline Treatment on Borrelia burgdorferi Phenotype John R. Caskey1,2†, Nicole R. Hasenkampf1, Dale S. Martin1, Vladimir N. Chouljenko2, Ramesh Subramanian2, Mercedes A. Cheslock1 and Monica E. Embers1* 1Division of Bacteriology and Parasitology, Tulane National Primate Research Center, Tulane University Health Sciences, Covington, LA, United States 2Division of Biotechnology and Molecular Medicine, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, United States Recent studies have shown that Borrelia burgdorferi can form antibiotic-tolerant persisters in the presence of microbiostatic drugs such as doxycycline. Precisely how this occurs is yet unknown. Our goal was to examine gene transcription by B. burgdorferi following doxycycline treatment in an effort to identify both persister-associated genes and possible targets for antimicrobial intervention. To do so, we performed next-generation RNA sequencing on doxycycline-treated spirochetes and treated spirochetes following regrowth, comparing them to untreated B. burgdorferi. A number of genes were perturbed and most of those which were statistically significant were down-regulated in the treated versus the untreated or treated/re-grown. Genes upregulated in the treated B. burgdorferi included a number of Erp genes and rplU, a 50S ribosomal protein. Among those genes associated with post-treatment regrowth were bba74 (Oms28), bba03, several...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

Related Links:

ConclusionsThe implication of these results is that policies aimed at reducing inequalities between residential neighborhoods are likely to beneficially affect all racial/ethnic groups but are not sufficient in reducing racial/ethnic disparities in intergenerational transmission of socio-economic status.
Source: Journal of Adolescence - Category: Child Development Source Type: research
The objective of this study was to examine the safety and functional properties of Staphylococcus simulans PMRS35 isolated from Budu. This strain possessed good lipase activity and moderate protease activity, tolerated 25% of salt, and did not exhibit decarboxylase activity. In addition, this strain displayed not only negative results towards pathogenic genes, hemolytic activity, and biofilm formation ability but also sensitivity to conventional antibiotics which included chloramphenicol, vancomycin, erythromycin, tetracycline, and gentamycin. Most importantly, the isolate maintained its viability towards simulated gastroi...
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
Publication date: Available online 19 February 2020Source: International Journal of Gastronomy and Food ScienceAuthor(s): Máirtín Mac Con Iomaire
Source: International Journal of Gastronomy and Food Science - Category: Food Science Source Type: research
Publication date: Available online 20 February 2020Source: Food HydrocolloidsAuthor(s): Bowen Yan, Xidong Jiao, Huaping Zhu, Qian Wang, Jianlian Huang, Jianxin Zhao, Hongwei Cao, Wenguo Zhou, Wenhai Zhang, Weijian Ye, Hao Zhang, Daming FanAbstractThe present study was conducted to elucidate the gelation mechanism of surimi protein-fish oil composite gels heated by microwave. Changes in chemical interactions, secondary structures, protein profiles and microwave-absorbing ability were investigated. The results clearly indicated that microwave heating could markedly (P 
Source: Food Hydrocolloids - Category: Food Science Source Type: research
Publication date: Available online 19 February 2020Source: Food ControlAuthor(s): John Burnett, Sophie Tongyu Wu, Henk C. den Bakker, Peter W. Cook, Deklin R. Veenhuizen, Susan R. Hammons, Manpreet Singh, Haley F. OliverAbstractThe purpose of this study was to determine the prevalence of Listeria monocytogenes and Salmonella enterica in retail produce environments and to elucidate possible ecological niches. Thirty environmental samples per store were collected during daily operations monthly for six months in 30 retail produce departments across seven states. Selected samples were serially diluted and plated to determine ...
Source: Food Control - Category: Food Science Source Type: research
In conclusion, unlike traditional alheiras which often contain pathogenic agents, no harmful organisms nor chemical hazards were found in these new products, even though produced by the same companies.
Source: Food Control - Category: Food Science Source Type: research
We report a case of a fatal Strongyloides stercoralis hyperinfection syndrome (SHS) in a migrant from Kenya, who had been living in Germany for three decades. A short-course oral steroid treatment for Chronic Obstructive Lung Disease (COPD) exacerbation had been administered four weeks prior to the presentation. The initial clinical and radiological findings suggested a mechanical small bowel obstruction as a cause of ileus. Our case highlights the importance of maintaining a high index of suspicion for strongyloidiasis in patients from endemic areas even years after they left the country of origin. It demonstrates that ev...
Source: Parasitology International - Category: Parasitology Source Type: research
Publication date: Available online 19 February 2020Source: Research in Veterinary ScienceAuthor(s): Ying-Feng Sun, Xuan Jiang, Ao Zhang, Ji-Fei Ma, Xiao-Xue Yu, Liu-An Li, Hai YuAbstractModified-live virus (MLV) vaccines derived from highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) were wildly used in China, which resulted in the emergence of MLV-like strains in pigs. Previous studies demonstrated that secondary bacterial infection could enhance HP-PRRSV infection-mediated inflammatory responses, but it is unknown whether early bacterial infection could enhance the HP-PRRSV MLV-like infectio...
Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research
Authors: Kikutani K, Itai J, Ota K, Chosa K, Yamane Y, Shime N Abstract Bronchial artery aneurysms are a rare but potentially life-threatening complications because of the massive hemothorax or hemoptysis that occurs with ruptures. A 79-year-old woman was transferred to our hospital because of the sudden onset of back pain, syncope, and subsequent hypotension. Computed tomography showed a left bronchial artery aneurysm with bilateral hemothorax and hemomediastinum. Transcatheter bronchial artery embolization failed because of the anatomical location, and she went into cardiopulmonary arrest. Cardiopulmonary resusci...
Source: Internal Medicine - Category: Internal Medicine Tags: Intern Med Source Type: research
Publication date: Available online 19 February 2020Source: Journal of Molecular BiologyAuthor(s): Iván Devesa-Guerra, Teresa Morales-Ruiz, Juan Pérez-Roldán, Jara Teresa Parrilla-Doblas, Macarena Dorado-León, María Victoria García-Ortiz, Rafael R. Ariza, Teresa Roldán-ArjonaAbstractTools for active targeted DNA demethylation are required to increase our knowledge about regulation and specific functions of this important epigenetic modification. DNA demethylation in mammals involve TET-mediated oxidation of 5-methylcytosine (5-meC), which may promote its replication-dependent...
Source: Journal of Molecular Biology - Category: Molecular Biology Source Type: research
More News: Academia | Amoxicillin | Arthritis | Biochemistry | Biotechnology | Borrelia | Cardiology | Ceftriaxone | Clinical Trials | Databases & Libraries | Doxycycline | Environmental Health | Fish | France Health | Funding | Gastroschisis Repair | Genetics | Germany Health | Grants | Heart | Infectious Diseases | International Medicine & Public Health | Italy Health | Laboratory Medicine | Legislation | Lyme Disease | Medical Ethics | Microbiology | Molecular Biology | Nanotechnology | Nutrition | Parasitology | Pathology | Reproduction Medicine | Rheumatology | Rocephin | Salmonella | Skin | Skin Biopsy | Statistics | Study | USA Health | Vaccines | Veterinary Research | Veterinary Vaccinations | Websites | Yale | Zoonoses