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Specialty: Molecular Biology
Condition: Diabetes

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Total 188 results found since Jan 2013.

Tripterygium glycoside suppresses epithelial ‑to‑mesenchymal transition of diabetic kidney disease podocytes by targeting autophagy through the mTOR/Twist1 pathway
Mol Med Rep. 2021 Aug;24(2):592. doi: 10.3892/mmr.2021.12231. Epub 2021 Jun 24.ABSTRACTTripterygium glycoside (TG) is a traditional Chinese medicine extract with immunosuppressive, anti‑inflammatory and anti‑renal fibrosis effects. Epithelial‑mesenchymal transition (EMT) and cell apoptosis are considered to be the major cause of podocyte injury in diabetic kidney disease (DKD). However, it remains unknown as to whether TG is able to alleviate podocyte injury to prevent DKD progression. Therefore, the present study aimed to clarify the podocyte protective effects of TG on DKD. TG, Twist1 small interfering RNA (siRNA) ...
Source: Molecular Medicine Reports - June 24, 2021 Category: Molecular Biology Authors: Mei Tao Danna Zheng Xudong Liang Diandian Wu Kang Hu Juan Jin Qiang He Source Type: research

Uncarboxylated osteocalcin alleviates the inhibitory effect of high glucose on osteogenic differentiation of mouse bone marrow-derived mesenchymal stem cells by regulating TP63
CONCLUSIONS: Our results indicate that GluOC reduces the inhibitory effect of high glucose on osteoblast differentiation by regulating the TP63/PTEN/Akt/GSK3β pathway. TP63 is a potential novel target for the prevention and treatment of diabetic osteoporosis.PMID:33906607 | DOI:10.1186/s12860-021-00365-7
Source: Mol Biol Cell - April 28, 2021 Category: Molecular Biology Authors: Fangzi Gong Le Gao Luyao Ma Guangxin Li Jianhong Yang Source Type: research

A novel role of kallikrein-related peptidase 8 in the pathogenesis of diabetic cardiac fibrosis
Conclusions: The present findings uncovered a novel pro-EndMT mechanism during the pathogenesis of diabetic cardiac fibrosis via the upregulation of KLK8, and may contribute to the development of future KLK8-based therapeutic strategies for diabetic cardiomyopathy.
Source: Theranostics - April 19, 2021 Category: Molecular Biology Authors: Jian-Kui Du, Qing Yu, Yu-Jian Liu, Shu-Fang Du, Li-Yang Huang, Dan-Hong Xu, Xin Ni, Xiao-Yan Zhu Tags: Research Paper Source Type: research

In pancreatic β-cells myosin 1b regulates glucose-stimulated insulin secretion by modulating an early step in insulin granule trafficking from the Golgi
Mol Biol Cell. 2021 Apr 7:mbcE21030094. doi: 10.1091/mbc.E21-03-0094. Online ahead of print.ABSTRACTPancreatic β-cells secrete insulin, which controls blood glucose levels, and defects in insulin secretion are responsible for diabetes mellitus. The actin cytoskeleton and some myosins support insulin granule trafficking and release, although a role for the class I myosin Myo1b, an actin- and membrane-associated load-sensitive motor, in insulin biology is unknown. We found by immunohistochemistry that Myo1b is expressed in islet cells of rat pancreas. In cultured rat insulinoma 832/13 cells Myo1b localized near actin patche...
Source: Mol Biol Cell - April 7, 2021 Category: Molecular Biology Authors: Hiroshi Tokuo Shigeru Komaba Lynne M Coluccio Source Type: research

Astragaloside IV inhibits palmitic acid-induced apoptosis through regulation of calcium homeostasis in mice podocytes.
Abstract Loss of podocytes is a hallmark of diabetic nephropathy, and a growing body of evidence indicates that podocytes are susceptible to palmitic acid (PA). We have previously shown that AS-IV inhibited PA-induced podocyte apoptosis by activating sarcoendoplasmic reticulum Ca2+ ATPase (SERCA), which indicate calcium regulation may involve in the process. Immunofluorescence staining, Western blot and flow cytometry were used to measure the protective efficacy of AS-IV to ameliorate PA-induced ER stress and podocyte apoptosis. Meanwhile, AS-IV inhibited cytochrome c release, decreased mitochondrial membrane pote...
Source: Molecular Biology Reports - February 19, 2021 Category: Molecular Biology Authors: Zang Y, Liu S, Cao A, Shan X, Deng W, Li Z, Wang H, Wang Y, Wang L, Peng W Tags: Mol Biol Rep Source Type: research

AMPK inhibits Smad3-mediated autoinduction of TGF- β1 in gastric cancer cells.
AMPK inhibits Smad3-mediated autoinduction of TGF-β1 in gastric cancer cells. J Cell Mol Med. 2021 Feb 03;: Authors: Zou J, Li C, Jiang S, Luo L, Yan X, Huang D, Luo Z Abstract We have previously shown that adenine monophosphate-activated protein kinase (AMPK) regulates transforming growth factor β (TGF-β)-triggered Smad3 phosphorylation. Here we report that AMPK inhibits TGF-β1 production. First, metformin reduced mRNA levels of TGF-β1 in gastric cancer cells, in parallel to the decrease of its protein abundance. The effects were more prominent in the cells containing LKB1, an upstream kinase of...
Source: J Cell Mol Med - February 3, 2021 Category: Molecular Biology Authors: Zou J, Li C, Jiang S, Luo L, Yan X, Huang D, Luo Z Tags: J Cell Mol Med Source Type: research

Osmotic regulation of aquaporin-8 expression in retinal pigment epithelial cells in vitro: Dependence on KATP channel activation.
Conclusions: The data indicate that hyperosmotic expression of AQP8 in RPE cells is dependent on the activation of KATP channels. The data suggest that AQP8 activity decreases the hypoxic VEGF expression and improves the viability of RPE cells which may have impact for ischemic retinal diseases like diabetic retinopathy and age-related macular degeneration. PMID: 33456300 [PubMed - in process]
Source: Molecular Vision - January 20, 2021 Category: Molecular Biology Tags: Mol Vis Source Type: research

Loureirin B activates GLP-1R and promotes insulin secretion in Ins-1 cells.
Abstract Loureirin B (LB) is a natural product derived from Sanguis draconis, which has hypoglycaemic effects. In order to research the possible target of LB in the treatment of diabetes, molecular docking was used to simulate the interaction between LB and potential targets, and among them, glucagon-like peptide-1 receptor (GLP-1R) had the optimal results. Further, spectroscopy and surface plasmon resonance (SPR) experiments were applied to detect the interaction between LB and GLP-1R. Ultimately, after GLP-1R siRNA interfering the expression of GLP-1R in Ins-1 cell, the promoting insulin secretion of LB was weak...
Source: J Cell Mol Med - December 10, 2020 Category: Molecular Biology Authors: Ding Y, Xia S, Zhang H, Chen Q, Niu B Tags: J Cell Mol Med Source Type: research

Exogenous sodium hydrosulfide protects against high glucose ‑induced injury and inflammation in human umbilical vein endothelial cells by inhibiting necroptosis via the p38 MAPK signaling pathway.
In conclusion, the findings of the present study indicated that NaHS may protect HUVECs against HG‑induced injury and inflammation by inhibiting necroptosis via the p38 MAPK signaling pathway, which may represent a promising drug for the therapy of diabetic vascular complications. PMID: 33215220 [PubMed - in process]
Source: Molecular Medicine Reports - November 21, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Distinct roles for Notch1 and Notch3 in human adipose-derived stem/stromal cell adipogenesis.
This study deepens the understanding of the Notch pathway by clarifying the distinct roles of Notch1 and Notch3 during adipogenesis. We showed that Notch3 is involved in early adipogenic differentiation, while Notch1 functions later in the process. In addition, we begin to uncover the interaction between the Notch and Wnt signaling pathways that may offer novel therapeutic targets aimed at obesity and diabetes. PMID: 33021719 [PubMed - as supplied by publisher]
Source: Molecular Biology Reports - October 5, 2020 Category: Molecular Biology Authors: Liu MC, Logan H, Newman JJ Tags: Mol Biol Rep Source Type: research

Loss of lncRNA MIAT ameliorates proliferation and fibrosis of diabetic nephropathy through reducing E2F3 expression.
In this study, we tested expression level of MIAT in DN patients and mesangial cells treated by high glucose (HG). Up-regulation of MIAT was observed in DN. Then, functional assays displayed that silence of MIAT by siRNA significantly repressed the proliferation and cycle progression in mesangial cells induced by HG. Meanwhile, we found that collagen IV, fibronectin and TGF-β1 protein expression was obviously triggered by HG, which could be rescued by loss of MIAT. Then, further assessment indicated that MIAT served as sponge harbouring miR-147a. Moreover, miR-147a was decreased in DN, which exhibited an antagonistic effe...
Source: J Cell Mol Med - October 2, 2020 Category: Molecular Biology Authors: Ji TT, Qi YH, Li XY, Tang B, Wang YK, Zheng PX, Li W, Qu X, Feng L, Bai SJ Tags: J Cell Mol Med Source Type: research

HDAC6-mediated α-tubulin deacetylation suppresses autophagy and enhances motility of podocytes in diabetic nephropathy.
Abstract Histone deacetylase 6 (HDAC6) is the specific subtype of HDACs which preferentially located in the cytoplasm, and is crucial in insulin signalling. However, the role of HDAC6 in type 2 diabetic nephropathy (DN) remains undefined. In current study, we observed that HDAC6 was markedly activated in the kidneys of type 2 diabetic patients and db/db mice with albuminuria, along with the advanced glycation end products (AGE)-treated podocytes. Selective inhibition of HDAC6 activity protected kidneys from hyperglycaemia in db/db mice. Notably, overexpressing HDAC6 inhibited autophagy and promoted motility aside ...
Source: J Cell Mol Med - September 3, 2020 Category: Molecular Biology Authors: Liang T, Qi C, Lai Y, Xie J, Wang H, Zhang L, Lin T, Jv M, Li J, Wang Y, Zhang Y, Chen Z, Qiu X, Li R, Li Z, Ye Z, Liu S, Liang X, Shi W, Wang W Tags: J Cell Mol Med Source Type: research

Tacrolimus ameliorates tubulointerstitial inflammation in diabetic nephropathy via inhibiting the NFATc1/TRPC6 pathway.
Abstract Tubulointerstitial inflammation is crucial for the progression of diabetic nephropathy (DN), and tubular cells act as a driving force in the inflammatory cascade. Emerging data suggested that tacrolimus (TAC) ameliorates podocyte injury and macrophage infiltration in streptozotocin (STZ) mice. However, the effect of TAC on tubulointerstitial inflammation remains unknown. We found that albuminuria and tubulointerstitial damage improved in db/db mice treated with TAC. Macrophage infiltration and expression of IL-6, TNF-α, fibronectin, collagen 1 and cleaved caspase 3 were inhibited as well. In addition, th...
Source: J Cell Mol Med - August 10, 2020 Category: Molecular Biology Authors: Zhang S, Wang H, Liu Y, Yang W, Liu J, Han Y, Liu Y, Liu F, Sun L, Xiao L Tags: J Cell Mol Med Source Type: research

Dysbiosis of intestinal microbiota mediates tubulointerstitial injury in diabetic nephropathy via the disruption of cholesterol homeostasis
Conclusion: Our studies for the first time demonstrated that the acetate produced from gut microbiota mediated the dysregulation of cholesterol homeostasis through the activation of GPR43, thereby contributing to the tubulointerstitial injury of DN, suggesting that gut microbiota reprogramming might be a new strategy for DN prevention and therapy.
Source: Theranostics - July 3, 2020 Category: Molecular Biology Authors: Ze Bo Hu, Jian Lu, Pei Pei Chen, Chen Chen Lu, Jia Xiu Zhang, Xue Qi Li, Ben Yin Yuan, Si Jia Huang, Xiong Zhong Ruan, Bi Cheng Liu, Kun Ling Ma Tags: Research Paper Source Type: research