Sequestration and Involucrum: Understanding Bone Necrosis and Revascularization in Pediatric Orthopedics
Orthop Clin North Am. 2024 Apr;55(2):233-246. doi: 10.1016/j.ocl.2023.09.005. Epub 2023 Oct 17.ABSTRACTSequestration, a condition where a section of bone becomes necrotic due to a loss of vascularity or thrombosis, can be a challenging complication of osteomyelitis. This review explores the pathophysiology of sequestration, highlighting the role of the periosteum in forming involucrum and creeping substitution which facilitate revascularization and bone formation. The authors also discuss the induced membrane technique, a two-stage surgical procedure for cases of failed healing of sequestration. Future directions include t...
Source: The Orthopedic Clinics of North America - February 25, 2024 Category: Orthopaedics Authors: Katherine S Hajdu Courtney E Baker Stephanie N Moore-Lotridge Jonathan G Schoenecker Source Type: research
Sequestration and Involucrum: Understanding Bone Necrosis and Revascularization in Pediatric Orthopedics
Orthop Clin North Am. 2024 Apr;55(2):233-246. doi: 10.1016/j.ocl.2023.09.005. Epub 2023 Oct 17.ABSTRACTSequestration, a condition where a section of bone becomes necrotic due to a loss of vascularity or thrombosis, can be a challenging complication of osteomyelitis. This review explores the pathophysiology of sequestration, highlighting the role of the periosteum in forming involucrum and creeping substitution which facilitate revascularization and bone formation. The authors also discuss the induced membrane technique, a two-stage surgical procedure for cases of failed healing of sequestration. Future directions include t...
Source: The Orthopedic Clinics of North America - February 25, 2024 Category: Orthopaedics Authors: Katherine S Hajdu Courtney E Baker Stephanie N Moore-Lotridge Jonathan G Schoenecker Source Type: research
The Differentiation Between Infection and Acute Charcot
Orthop Clin North Am. 2024 Apr;55(2):299-309. doi: 10.1016/j.ocl.2023.08.002. Epub 2023 Sep 7.ABSTRACTThe differentiation between acute Charcot neuroarthropathy and infection in the foot and ankle should be supported by multiple criteria. A detailed history and physical examination should always be completed. Plain radiographs should be performed, though advanced imaging, currently MRI, is more helpful in diagnosis. Scintigraphy and PET may become the standard imaging modalities once they are more clinically available due to their reported increased accuracy. Laboratory analysis can also act as a helpful diagnostic tool. H...
Source: The Orthopedic Clinics of North America - February 25, 2024 Category: Orthopaedics Authors: Ryan G Rogero Samhita Swamy Clayton C Bettin Source Type: research
The Differentiation Between Infection and Acute Charcot
Orthop Clin North Am. 2024 Apr;55(2):299-309. doi: 10.1016/j.ocl.2023.08.002. Epub 2023 Sep 7.ABSTRACTThe differentiation between acute Charcot neuroarthropathy and infection in the foot and ankle should be supported by multiple criteria. A detailed history and physical examination should always be completed. Plain radiographs should be performed, though advanced imaging, currently MRI, is more helpful in diagnosis. Scintigraphy and PET may become the standard imaging modalities once they are more clinically available due to their reported increased accuracy. Laboratory analysis can also act as a helpful diagnostic tool. H...
Source: The Orthopedic Clinics of North America - February 25, 2024 Category: Orthopaedics Authors: Ryan G Rogero Samhita Swamy Clayton C Bettin Source Type: research
Sequestration and Involucrum: Understanding Bone Necrosis and Revascularization in Pediatric Orthopedics
Orthop Clin North Am. 2024 Apr;55(2):233-246. doi: 10.1016/j.ocl.2023.09.005. Epub 2023 Oct 17.ABSTRACTSequestration, a condition where a section of bone becomes necrotic due to a loss of vascularity or thrombosis, can be a challenging complication of osteomyelitis. This review explores the pathophysiology of sequestration, highlighting the role of the periosteum in forming involucrum and creeping substitution which facilitate revascularization and bone formation. The authors also discuss the induced membrane technique, a two-stage surgical procedure for cases of failed healing of sequestration. Future directions include t...
Source: The Orthopedic Clinics of North America - February 25, 2024 Category: Orthopaedics Authors: Katherine S Hajdu Courtney E Baker Stephanie N Moore-Lotridge Jonathan G Schoenecker Source Type: research
Sequestration and Involucrum: Understanding Bone Necrosis and Revascularization in Pediatric Orthopedics
Orthop Clin North Am. 2024 Apr;55(2):233-246. doi: 10.1016/j.ocl.2023.09.005. Epub 2023 Oct 17.ABSTRACTSequestration, a condition where a section of bone becomes necrotic due to a loss of vascularity or thrombosis, can be a challenging complication of osteomyelitis. This review explores the pathophysiology of sequestration, highlighting the role of the periosteum in forming involucrum and creeping substitution which facilitate revascularization and bone formation. The authors also discuss the induced membrane technique, a two-stage surgical procedure for cases of failed healing of sequestration. Future directions include t...
Source: The Orthopedic Clinics of North America - February 25, 2024 Category: Orthopaedics Authors: Katherine S Hajdu Courtney E Baker Stephanie N Moore-Lotridge Jonathan G Schoenecker Source Type: research
The Differentiation Between Infection and Acute Charcot
Orthop Clin North Am. 2024 Apr;55(2):299-309. doi: 10.1016/j.ocl.2023.08.002. Epub 2023 Sep 7.ABSTRACTThe differentiation between acute Charcot neuroarthropathy and infection in the foot and ankle should be supported by multiple criteria. A detailed history and physical examination should always be completed. Plain radiographs should be performed, though advanced imaging, currently MRI, is more helpful in diagnosis. Scintigraphy and PET may become the standard imaging modalities once they are more clinically available due to their reported increased accuracy. Laboratory analysis can also act as a helpful diagnostic tool. H...
Source: The Orthopedic Clinics of North America - February 25, 2024 Category: Orthopaedics Authors: Ryan G Rogero Samhita Swamy Clayton C Bettin Source Type: research
Sequestration and Involucrum: Understanding Bone Necrosis and Revascularization in Pediatric Orthopedics
Orthop Clin North Am. 2024 Apr;55(2):233-246. doi: 10.1016/j.ocl.2023.09.005. Epub 2023 Oct 17.ABSTRACTSequestration, a condition where a section of bone becomes necrotic due to a loss of vascularity or thrombosis, can be a challenging complication of osteomyelitis. This review explores the pathophysiology of sequestration, highlighting the role of the periosteum in forming involucrum and creeping substitution which facilitate revascularization and bone formation. The authors also discuss the induced membrane technique, a two-stage surgical procedure for cases of failed healing of sequestration. Future directions include t...
Source: The Orthopedic Clinics of North America - February 25, 2024 Category: Orthopaedics Authors: Katherine S Hajdu Courtney E Baker Stephanie N Moore-Lotridge Jonathan G Schoenecker Source Type: research
The Differentiation Between Infection and Acute Charcot
Orthop Clin North Am. 2024 Apr;55(2):299-309. doi: 10.1016/j.ocl.2023.08.002. Epub 2023 Sep 7.ABSTRACTThe differentiation between acute Charcot neuroarthropathy and infection in the foot and ankle should be supported by multiple criteria. A detailed history and physical examination should always be completed. Plain radiographs should be performed, though advanced imaging, currently MRI, is more helpful in diagnosis. Scintigraphy and PET may become the standard imaging modalities once they are more clinically available due to their reported increased accuracy. Laboratory analysis can also act as a helpful diagnostic tool. H...
Source: The Orthopedic Clinics of North America - February 25, 2024 Category: Orthopaedics Authors: Ryan G Rogero Samhita Swamy Clayton C Bettin Source Type: research
P2RX7 gene variants associate with altered inflammasome assembly and reduced pyroptosis in chronic nonbacterial osteomyelitis (CNO)
This study investigated CNO-associated variants in P2RX7, encoding for the ATP-dependent trans-membrane K+ channel P2X7, and their effects on NLRP3 inflammasome assembly. Whole exome sequencing in two related transgenerational CNO patients, and target sequencing of P2RX7 in a large CNO cohort (N = 190) were conducted. Results were compared with publicly available datasets and regional controls (N = 1873). Findings were integrated with demographic and clinical data. Patient-derived monocytes and genetically modified THP-1 cells were used to investigate potassium flux, inflammasome assembly, pyroptosis, and cytokine release....
Source: Journal of Autoimmunity - February 24, 2024 Category: Allergy & Immunology Authors: Amandine Charras Sigrun R Hofmann Allison Cox Felix Schulze Susanne Russ Sarah Northey Xuan Liu Yongxiang Fang Sam Haldenby Hella Hartmann Alexander G Bassuk Ana Carvalho Francesca Sposito Lev Grinstein Angela R ösen-Wolff Almut Meyer-Bahlburg Michael W Source Type: research
P2RX7 gene variants associate with altered inflammasome assembly and reduced pyroptosis in chronic nonbacterial osteomyelitis (CNO)
This study investigated CNO-associated variants in P2RX7, encoding for the ATP-dependent trans-membrane K+ channel P2X7, and their effects on NLRP3 inflammasome assembly. Whole exome sequencing in two related transgenerational CNO patients, and target sequencing of P2RX7 in a large CNO cohort (N = 190) were conducted. Results were compared with publicly available datasets and regional controls (N = 1873). Findings were integrated with demographic and clinical data. Patient-derived monocytes and genetically modified THP-1 cells were used to investigate potassium flux, inflammasome assembly, pyroptosis, and cytokine release....
Source: Journal of Autoimmunity - February 24, 2024 Category: Allergy & Immunology Authors: Amandine Charras Sigrun R Hofmann Allison Cox Felix Schulze Susanne Russ Sarah Northey Xuan Liu Yongxiang Fang Sam Haldenby Hella Hartmann Alexander G Bassuk Ana Carvalho Francesca Sposito Lev Grinstein Angela R ösen-Wolff Almut Meyer-Bahlburg Michael W Source Type: research
P2RX7 gene variants associate with altered inflammasome assembly and reduced pyroptosis in chronic nonbacterial osteomyelitis (CNO)
This study investigated CNO-associated variants in P2RX7, encoding for the ATP-dependent trans-membrane K+ channel P2X7, and their effects on NLRP3 inflammasome assembly. Whole exome sequencing in two related transgenerational CNO patients, and target sequencing of P2RX7 in a large CNO cohort (N = 190) were conducted. Results were compared with publicly available datasets and regional controls (N = 1873). Findings were integrated with demographic and clinical data. Patient-derived monocytes and genetically modified THP-1 cells were used to investigate potassium flux, inflammasome assembly, pyroptosis, and cytokine release....
Source: Journal of Autoimmunity - February 24, 2024 Category: Allergy & Immunology Authors: Amandine Charras Sigrun R Hofmann Allison Cox Felix Schulze Susanne Russ Sarah Northey Xuan Liu Yongxiang Fang Sam Haldenby Hella Hartmann Alexander G Bassuk Ana Carvalho Francesca Sposito Lev Grinstein Angela R ösen-Wolff Almut Meyer-Bahlburg Michael W Source Type: research
P2RX7 gene variants associate with altered inflammasome assembly and reduced pyroptosis in chronic nonbacterial osteomyelitis (CNO)
This study investigated CNO-associated variants in P2RX7, encoding for the ATP-dependent trans-membrane K+ channel P2X7, and their effects on NLRP3 inflammasome assembly. Whole exome sequencing in two related transgenerational CNO patients, and target sequencing of P2RX7 in a large CNO cohort (N = 190) were conducted. Results were compared with publicly available datasets and regional controls (N = 1873). Findings were integrated with demographic and clinical data. Patient-derived monocytes and genetically modified THP-1 cells were used to investigate potassium flux, inflammasome assembly, pyroptosis, and cytokine release....
Source: Journal of Autoimmunity - February 24, 2024 Category: Allergy & Immunology Authors: Amandine Charras Sigrun R Hofmann Allison Cox Felix Schulze Susanne Russ Sarah Northey Xuan Liu Yongxiang Fang Sam Haldenby Hella Hartmann Alexander G Bassuk Ana Carvalho Francesca Sposito Lev Grinstein Angela R ösen-Wolff Almut Meyer-Bahlburg Michael W Source Type: research
P2RX7 gene variants associate with altered inflammasome assembly and reduced pyroptosis in chronic nonbacterial osteomyelitis (CNO)
This study investigated CNO-associated variants in P2RX7, encoding for the ATP-dependent trans-membrane K+ channel P2X7, and their effects on NLRP3 inflammasome assembly. Whole exome sequencing in two related transgenerational CNO patients, and target sequencing of P2RX7 in a large CNO cohort (N = 190) were conducted. Results were compared with publicly available datasets and regional controls (N = 1873). Findings were integrated with demographic and clinical data. Patient-derived monocytes and genetically modified THP-1 cells were used to investigate potassium flux, inflammasome assembly, pyroptosis, and cytokine release....
Source: Journal of Autoimmunity - February 24, 2024 Category: Allergy & Immunology Authors: Amandine Charras Sigrun R Hofmann Allison Cox Felix Schulze Susanne Russ Sarah Northey Xuan Liu Yongxiang Fang Sam Haldenby Hella Hartmann Alexander G Bassuk Ana Carvalho Francesca Sposito Lev Grinstein Angela R ösen-Wolff Almut Meyer-Bahlburg Michael W Source Type: research
P2RX7 gene variants associate with altered inflammasome assembly and reduced pyroptosis in chronic nonbacterial osteomyelitis (CNO)
This study investigated CNO-associated variants in P2RX7, encoding for the ATP-dependent trans-membrane K+ channel P2X7, and their effects on NLRP3 inflammasome assembly. Whole exome sequencing in two related transgenerational CNO patients, and target sequencing of P2RX7 in a large CNO cohort (N = 190) were conducted. Results were compared with publicly available datasets and regional controls (N = 1873). Findings were integrated with demographic and clinical data. Patient-derived monocytes and genetically modified THP-1 cells were used to investigate potassium flux, inflammasome assembly, pyroptosis, and cytokine release....
Source: Journal of Autoimmunity - February 24, 2024 Category: Allergy & Immunology Authors: Amandine Charras Sigrun R Hofmann Allison Cox Felix Schulze Susanne Russ Sarah Northey Xuan Liu Yongxiang Fang Sam Haldenby Hella Hartmann Alexander G Bassuk Ana Carvalho Francesca Sposito Lev Grinstein Angela R ösen-Wolff Almut Meyer-Bahlburg Michael W Source Type: research
