Digital spatial profiling application in breast cancer: a user ’s perspective
We present the application of this technology to breast cancer samples. Applying the ‘off the shelf’ cancer panel and a custom-conjugated E-cadher in antibody, we quantify vast intra-tumoural heterogeneity in immunological and tumour markers, and demonstrate a need for focussed selection of target cell populations. The technology offers enormous potential not only for making research advances but also for improving standard operating procedure s in diagnostic applications. (Source: Virchows Archiv)
Source: Virchows Archiv - May 26, 2020 Category: Pathology Source Type: research

Testing for ROS1 , ALK , MET , and HER2 rearrangements and amplifications in a large series of biliary tract adenocarcinomas
AbstractBiliary tract carcinomas are divided into intrahepatic, perihilar, distal extrahepatic cholangiocarcinomas, and gallbladder adenocarcinomas. Therapies targetingROS1,ALK,MET, andHER2 alterations are currently evaluated in clinical trials. We assessedROS1 andALK translocations/amplifications as well asMET andHER2 amplifications for each tumor subtype by fluorescent in situ hybridization (FISH) and immunohistochemistry (IHC) in 73 intrahepatic, 40 perihilar bile duct, 36 distal extrahepatic cholangiocarcinomas, and 45 gallbladder adenocarcinomas (n = 194). By FISH, we detected targetable alterations in 5...
Source: Virchows Archiv - May 24, 2020 Category: Pathology Source Type: research

Novel insights into the mixed germ cell-sex cord stromal tumor of the testis: detection of chromosomal aneuploidy and further morphological evidence supporting the neoplastic nature of the germ cell component
AbstractThe existence of a true mixed germ cell-sex cord stromal tumor (MGSCT) of the testis remains controversial. Based on our experience with rare testicular tumors in this spectrum, we sought to perform a detailed clinicopathologic and molecular study of MGCSCT. Eight cases of testicular MGSCT were morphologically reviewed, screened for chromosomal aberrations (using array comparative genomic hybridization (aCGH) and low pass genomic sequencing), and analyzed by next generation sequencing (The Illumina TruSight Tumor 170). Immunohistochemistry for OCT3/4, Nanog, SALL4, DMRT1, and inhibin was performed on the cohort. Cl...
Source: Virchows Archiv - May 23, 2020 Category: Pathology Source Type: research

The SWI/SNF chromatin-remodeling complex status in renal cell carcinomas with sarcomatoid or rhabdoid features
AbstractThe presence of sarcomatoid or rhabdoid features (which are associated with advanced disease and poor prognosis) is rarely observed in the subtypes of renal cell carcinoma (RCC). The SWI/SNF chromatin-remodeling complex, which is composed of evolutionarily conserved core subunits includingSMARCB1/INI1 (SMARCB1),SMARCA4/BRG1 (SMARCA4),SMARCC1/BAF155 (SMARCC1), andSMARCC2/BAF170 (SMARCC2), can be regarded as the prototype of an epigenetic regulator of gene expression that is involved in tumor suppression. We analyzed the histological, immunohistochemical, and clinicopathological status in 72 cases of RCC with sarcoma...
Source: Virchows Archiv - May 23, 2020 Category: Pathology Source Type: research

Prognostic relevance of the loss of stromal CD34 positive fibroblasts in invasive lobular carcinoma of the breast
AbstractCD34+ fibroblasts are constitutive stromal components of virtually all organs, including the mammary stroma, being involved in matrix synthesis, antigen presentation, and tumor-associated stromal remodeling. The most common subtype of invasive breast carcinoma, invasive carcinoma of no special type (IBC-NST), is known for its stromal loss of CD34+ fibroblasts while acquiring alpha smooth muscle actin-positive ( α-SMA+) myofibroblasts, i.e., cancer-associated fibroblasts (CAF), whereas invasive lobular carcinoma (ILC) displays partial preservation of CD34+ fibroblasts. The aim of this study was to evaluate the...
Source: Virchows Archiv - May 20, 2020 Category: Pathology Source Type: research

Prevalence of human papillomavirus and implication on survival in Chinese penile cancer
AbstractWe assessed the prevalence of HPV DNA in a large series of Chinese penile cancer and examine its association with the histological subtype, p16INK4a expression, and prognosis. We pathologically categorized 226 invasive penile squamous cell carcinomas and assessed HPV genotyping by real-time PCR and p16INK4a immunohistochemistry. The results were correlated with histopathological and clinical parameters and disease-specific survival (DSS). HPV DNA was detected in 32.7% (74/226) of penile cancer cases. The most frequent genotype was HPV 16 (64/74, 86.5%), followed by HPV 18 (6/74, 8.1%). Fifty-nine (26.1%) cases were...
Source: Virchows Archiv - May 19, 2020 Category: Pathology Source Type: research

The prognostic role of tissue TLR2 and TLR4 in colorectal cancer
This study aimed to explore the prognostic value of TLR2 and TLR4 tumor expressions in colorectal cancer patients. We studied the immunohistochemical expressions of TLR2 and TLR4 using tissue microarray specimens from 825 patients undergoing surgery in the Department of Surgery , Helsinki University Hospital, between 1982 and 2002. We assessed the relationships between TLR2 and TLR4 expressions and clinicopathological variables and patient survival. We generated survival curves using the Kaplan-Meier method, determining significance with the log-rank test. Among patients w ith lymph node–positive disease and no dista...
Source: Virchows Archiv - May 19, 2020 Category: Pathology Source Type: research

Negative prognostic impact of PD-L1 expression in tumor cells of undifferentiated (anaplastic) carcinoma with osteoclast-like giant cells of the pancreas: study of 13 cases comparing ductal pancreatic carcinoma and review of the literature
In this study, we collected, retrospectively, 13 cases of well-documented UCOGCs and performed immunohistochemistry focused on the expression of the programmed death-ligand 1 (PD-L1) and several other potential therapeutic and predictive markers (PanTRK, p53, MSH2, PMS2, and the number of tumor-infiltrating lymphocytes), to explore their correlation with the follow-up of the patients. As a control group, we examined 24 cases of conventional pancreatic ductal adenocarcinoma (PDAC). In our results, PanTRK was negative in all 24 cases. P53 did not show any significant differences between UCOGCs and PDACs, and the entire cohor...
Source: Virchows Archiv - May 18, 2020 Category: Pathology Source Type: research

In this issue
(Source: Virchows Archiv)
Source: Virchows Archiv - May 18, 2020 Category: Pathology Source Type: research

Personalized histopathology reporting for personalized medicine: a plea for improved communication
(Source: Virchows Archiv)
Source: Virchows Archiv - May 16, 2020 Category: Pathology Source Type: research

Upregulation of the heterogeneous nuclear ribonucleoprotein hnRNPA1 is an independent predictor of early biochemical recurrence in TMPRSS2:ERG fusion-negative prostate cancers
AbstractHeterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) is a ubiquitous RNA splicing factor that is overexpressed and prognostically relevant in various human cancer types. To study the impact of hnRNPA1 expression in prostate cancer, we analyzed a tissue microarray containing 17,747 clinical prostate cancer specimens by immunohistochemistry. hnRNPA1 was expressed in normal prostate glandular cells but often overexpressed in cancer cells. hnRNPA1 immunostaining was interpretable in 14,258 cancers and considered strong in 33.4%, moderate in 45.9%, weak in 15.3%, and negative in 5.4%. Moderate to strong hnRNPA1 immunost...
Source: Virchows Archiv - May 16, 2020 Category: Pathology Source Type: research

Rare MDM2 amplification in a fat-predominant angiomyolipoma
We describe our findings in a series of 35 AMLs including epithelioid, fat-poor, and fat-predominant variants, following interrogation of theMDM2 locus by FISH and CISH assays.MDM2 amplification was detected in 1 fat-predominant AML. Our findings demonstrate that rareMDM2 amplifications can occur in AMLs. We favor that this finding likely represents a “molecular bystander” event since these tumors are mainly driven by aberrations in theTSC1/TSC2 genes. Nevertheless, the presence ofMDM2 amplification in a fat-predominant AML could present a potential diagnostic pitfall, particularly when confronted with the diff...
Source: Virchows Archiv - May 15, 2020 Category: Pathology Source Type: research

Duodenal bulb biopsy in the diagnostic work-up of coeliac disease
AbstractCoeliac disease (CD) is an autoimmune enteropathy which can present with patchy mucosal lesions. The aim of the present study is to investigate the significance of duodenal bulb biopsy in the diagnostic work-up of CD in both pediatric and adult patients, and to highlight the key points for pathologists. D1 (duodenal bulb) and D2 (distal duodenum) biopsies of 153 newly diagnosed serology-positive CD patients were evaluated for villous/crypt ratio and intraepithelial lymphocyte (IEL) counts on CD3-stained slides and were classified according to Marsh. Mucosal pathology was patchy in 15% (13% only D1 and 2% only D2) o...
Source: Virchows Archiv - May 13, 2020 Category: Pathology Source Type: research

Gastric- and intestinal-type IPMN: two of a kind?
(Source: Virchows Archiv)
Source: Virchows Archiv - May 12, 2020 Category: Pathology Source Type: research

Got nerve? Autonomic innervation of the human liver
(Source: Virchows Archiv)
Source: Virchows Archiv - May 11, 2020 Category: Pathology Source Type: research

Biologic profiles meet clinical priorities: incorporating pseudopyloric, and spasmolytic-expressing metaplasia in the assessment of gastric atrophy
(Source: Virchows Archiv)
Source: Virchows Archiv - May 11, 2020 Category: Pathology Source Type: research

Expression and prognostic significance of pyruvate dehydrogenase kinase 1 in bladder urothelial carcinoma
AbstractMuscular infiltrating bladder urothelial carcinoma (MIBC) is a highly malignant disease with a poor prognosis. Radical cystectomy is the standard treatment. However, due to surgery and postoperative complications, the quality of life of patients is seriously affected. Therefore, it is increasingly important to find prognostic markers and new therapeutic targets for MIBC. Here, we investigated the expression of PDK1, a key regulator of glucose metabolism, in bladder urothelial carcinoma (BLCA) and its effect on prognosis. The expression pattern of PDK1 was examined by bioinformatics analysis and immunohistochemistry...
Source: Virchows Archiv - May 9, 2020 Category: Pathology Source Type: research

The differential expression of micro-RNAs 21, 200c, 204, 205, and 211 in benign, dysplastic and malignant melanocytic lesions and critical evaluation of their role as diagnostic biomarkers
In conclusion, we have validated some miRNAs, including miRNA 21 and 211, as potential diagnostic biomarkers of benign, dysplastic, and malignant melanocytic lesions. However, we also highlight the crucial importance of considering tissue morphology and spatial expression patterns when using molecular techniques for the discovery and validation of new biomarkers. (Source: Virchows Archiv)
Source: Virchows Archiv - May 9, 2020 Category: Pathology Source Type: research

Heavy and light chain (AHL)-type cardiac amyloidosis: first histopathologic-proven case illustrating involvement of the heart
We present briefly the case of an 80-year-old man with concern for cardiac amyloidosis and a subsequent endomyocardial biopsy revealing significant deposition of amorphous Congo red-positive material. By immunofluorescence microscopy, the amyloidogenic material showed positive expression for IgG heavy chain and kappa light chain, with negative staining for IgM and IgA heavy chains and lambda light chain supporting a diagnosis of heavy and light chain (AHL)-type amyloidosis. Immunofluorescence staining for the IgG heavy chain subclasses supported and further classified the patient ’s AHL-type cardiac amyloidosis as be...
Source: Virchows Archiv - May 9, 2020 Category: Pathology Source Type: research

Correction to: Abstracts
The authors of the abstract PS-13-082 (page S192) request the addition of another affiliation. This is for Dr Shereen El-Mashed. Her affiliation is listed as ‘Beaumont Hospital, Pathology, Dublin, Ireland. She needs to have the additional affiliation added. ‘Lecturer in Histopathology, National Liver Institute, Menoufia University, Egypt’. All authors consent to this amendment. (Source: Virchows Archiv)
Source: Virchows Archiv - May 8, 2020 Category: Pathology Source Type: research

Factors influencing agreement of breast cancer luminal molecular subtype by Ki67 labeling index between core needle biopsy and surgical resection specimens
AbstractReliable determination of Ki67 labeling index (Ki67-LI) on core needle biopsy (CNB) is essential for determining breast cancer molecular subtype for therapy planning. However, studies on agreement between molecular subtype and Ki67-LI between CNB and surgical resection (SR) specimens are conflicting. The present study analyzed the influence of clinicopathological and sampling-associated factors on agreement. Molecular subtype was determined visually by Ki67-LI in 484 pairs of CNB and SR specimens of invasive estrogen receptor (ER) –positive, human epidermal growth factor (HER2)–negative breast cancer. L...
Source: Virchows Archiv - May 7, 2020 Category: Pathology Source Type: research

Clinicopathological study of intraductal carcinoma of the salivary gland, with emphasis on the apocrine type
In this study, immunohistochemistry and mutation analyses (includingHRAS/PIK3CA mutations,RET rearrangement, and human epidermal growth factor receptor 2 [HER2] amplification) of 9 ICs (including 3 pure ICs, 6 ICs with invasive carcinoma) and 24 conventional SDCs were performed and the results were compared. Four intercalated duct-type cases were positive for SOX10 and S100 and negative for AR; five apocrine-type cases showed opposite results. All five apocrine-type cases had cysts with relatively circumscribed tumor borders and morphologically mimicking breast low-grade ductal carcinoma in situ or papillary carcinoma.RET ...
Source: Virchows Archiv - May 7, 2020 Category: Pathology Source Type: research

Role of liver biopsy in the era of clinical prediction scores for “drug-induced liver injury” (DILI): experience of a tertiary referral hospital
AbstractThe clinical implications of the biopsy findings in cases of drug-induced liver injury (DILI) are not fully elucidated. The aim of this study was to evaluate the histopathological findings of cases diagnosed as DILI and to correlate them with clinical and biochemical findings (such as causality assessment algorithms). We searched our department database for all cases of liver biopsy with findings consistent with toxic liver disease and selected those with a clinical diagnosis of DILI. The causative relationships were established according to Roussel Uclaf Causality Assessment Method (RUCAM). A total of 53 cases of ...
Source: Virchows Archiv - May 6, 2020 Category: Pathology Source Type: research

Frequent KIT mutations in skin lesions of patients with BRAF wild-type Langerhans cell histiocytosis
AbstractLangerhans cell histiocytosis (LCH) is characterized by mutations of the RAS-RAF-MAPK signaling pathway. We analyzed MAP2K1, NRAS and KIT mutation incidence in skin lesions of BRAF wild-type (wt) LCH patients. We evaluated the occurrence of MAP2K1, NRAS and KIT mutations in seven LCH and one indeterminate cell histiocytosis (ICH) patients. MAP2K1 mutation frequency was found to be 3/7 (42.9%) in LCH and also found in ICH. Similarly, the KIT mutation frequency was found to be equally prevalent (4/7, 57.1%) in LCH and also occurred in ICH. Involvement of KIT exons in LCH-ICH indicated that exon 9/11/18 were equally p...
Source: Virchows Archiv - May 5, 2020 Category: Pathology Source Type: research

Occult primary cardiac lymphomas causing unexpected/sudden death or acute heart failure
AbstractThree cases of unexpected/sudden death (N = 2) or acute heart failure (N = 1) were investigated in our centre. The first patient died unexpectedly after surgery for cardiac tamponade and constrictive pericarditis; at autopsy, gross features mimicked a pericardial mesothelioma. The second patient died suddenly after recovering from a respiratory insufficiency episode ; autopsy revealed an epicardial mass encircling the right coronary artery. The third patient presenting symptoms mimicked a fulminant myocarditis and she underwent endomyocardial biopsy. In all cases, histology disclosed a d...
Source: Virchows Archiv - May 5, 2020 Category: Pathology Source Type: research

In this issue
(Source: Virchows Archiv)
Source: Virchows Archiv - May 4, 2020 Category: Pathology Source Type: research

Topography, morphology, and etiology of lymphocytic gastritis: a focus on celiac disease
(Source: Virchows Archiv)
Source: Virchows Archiv - May 4, 2020 Category: Pathology Source Type: research

Early histologic findings of pulmonary SARS-CoV-2 infection detected in a surgical specimen
AbstractDespite the current pandemic season, reports on pathologic features of coronavirus disease 19 (Covid-19) are exceedingly rare at the present time. Here we describe the pathologic features of early lung involvement by Covid-19 in a surgical sample resected for carcinoma from a patient who developed SARS-CoV-2 infection soon after surgery. The main histologic findings observed were pneumocyte damage, alveolar hemorrhages with clustering of macrophages, prominent and diffuse neutrophilic margination within septal vessels, and interstitial inflammatory infiltrates, mainly represented by CD8+ T lymphocytes. These featur...
Source: Virchows Archiv - April 30, 2020 Category: Pathology Source Type: research

Histological changes associated with pyloric and pseudopyloric metaplasia after Helicobacter pylori eradication
In conclusion, PM and PPM are considered different modulations of the same line of differentiation, which are both reversible, with PM potentially emerging from PPM upon progression. (Source: Virchows Archiv)
Source: Virchows Archiv - April 30, 2020 Category: Pathology Source Type: research

The autopsy debate during the COVID-19 emergency: the Italian experience
(Source: Virchows Archiv)
Source: Virchows Archiv - April 29, 2020 Category: Pathology Source Type: research

Expression of potential therapeutic target SSTR2a in primary and metastatic non-keratinizing nasopharyngeal carcinoma
AbstractSomatostatin receptor 2a (SSTR2a) is an important diagnostic and scintigraphic marker in several tumors, as well as a potential therapeutic target. However, the expression and clinicopathologic significance of SSTR2a in nasopharyngeal carcinoma (NPC) remain unknown. The expression of SSTR2a was retrospectively analyzed in a large series of NPC tissue samples (106 primary NPC samples, comprising 99 primary non-keratinizing NPC (NK-NPC) and 7 keratinizing NPC (K-NPC) samples, and 41 metastatic NPC samples) by immunohistochemistry, with 24 cases of normal nasopharyngeal mucosa tissues used as a control group. Normal e...
Source: Virchows Archiv - April 23, 2020 Category: Pathology Source Type: research

CD20-positive extranodal NK/T cell lymphoma: clinicopathologic and prognostic features
AbstractExtranodal natural killer (NK)/T cell lymphoma (ENKTCL) with aberrant CD20 expression is extremely rare. Here, we describe the clinicopathologic features of 11 CD20-positive ENKTCLs from three institutions in China along with a literature review. Membranous expression of CD20 was identified in 1.29% (11/851) of ENKTCLs. CD20-positive ENKTCLs primarily occurred in extra-nasal sites (72.2%, 13/18) rather than in the nasal cavity (27.8%, 5/18). Most evaluated patients (71.4%, 10/14) presented ENKTCL at advanced stage IV. The percentage of CD20-positive tumor cells ranged from 20 to 90%, and the CD20 staining intensity...
Source: Virchows Archiv - April 20, 2020 Category: Pathology Source Type: research

Identification of CTNNB1-PLAG1 gene rearrangement in a patient with pulmonary pleomorphic adenoma
AbstractPulmonary pleomorphic adenoma (PA) is a rare salivary gland-type neoplasm, which predominantly occurs in the proximal airway. Rearrangement of the pleomorphic adenoma gene 1 (PLAG1) is the most frequent genetic event in PAs of salivary glands. However, whether pulmonary PA also harborsPLAG1 rearrangement has not been elucidated. Here, we present a case of pulmonary PA, located at the middle lobar bronchus, in a 54-year-old man.CTNNB1-PLAG1 gene fusion was identified by reverse transcription-polymerase chain reaction using formalin-fixed paraffin-embedded tissue (FFPE). Furthermore, immunohistochemical analysis reve...
Source: Virchows Archiv - April 19, 2020 Category: Pathology Source Type: research

Complement activation is associated with crescent formation in IgA nephropathy
This study included 132 IgAN patients diagnosed by renal biopsies. The clinical parameters at the time of the biopsies were obtained from patient data records. We classified the patients into C  ≥ 1 and C0 groups, and compared clinical, light microscopic, and immunofluorescent features. In the C ≥ 1 group, 2 (1.5%) and 31 (23.5%) patients were assigned to C2 and C1, respectively. The remaining 99 patients (75%) were classified as C0. The C ≥ 1 group had lower average age and rate of hypertension, and higher score of urinary occult blood and E score. The C ≥&thins...
Source: Virchows Archiv - April 16, 2020 Category: Pathology Source Type: research

Pleuropulmonary blastoma type I and congenital pulmonary airway malformation type 4: distinct entities or sides of the same coin?
(Source: Virchows Archiv)
Source: Virchows Archiv - April 16, 2020 Category: Pathology Source Type: research

pT1 high-grade bladder cancer: histologic criteria, pitfalls in the assessment of invasion, and substaging
AbstractMost patients with bladder carcinoma are diagnosed with non-muscle-invasive disease, stage Ta, and pT1. Stage remains as the single most important prognostic indicator in urothelial carcinoma. Among the pT1 bladder cancer patients, recurrence and progression of disease occur in 50% and 10%, respectively. The identification of high-risk patients within the pT1 subgroup remains an important clinical goal and an active field of research. Substaging of pT1 disease has been claimed as important histologic discriminator by the 2016 World Health Organization (WHO) classification of the genitourinary tract tumors and by th...
Source: Virchows Archiv - April 15, 2020 Category: Pathology Source Type: research

Utility of DNA flow cytometry in distinguishing between malignant and benign intrahepatic biliary lesions
In conclusion, the frequency of aneuploidy was significantly higher in iCCA (47%) than in its benign morphological mimics (0%), suggesting that it may potentially serve as a diagnostic marker of malignancy in challenging situations. Our findings also suggest that most BDAs and BDHs, if not all, are benign entities and may not represent precursor lesions to iCCAs that often harbor aneuploidy. Although a larger cohort will be necessary to further determine the prognostic significance of aneuploidy in iCCA patients after resection, the patients with aneup loid tumors may have a higher risk for tumor progression, especially du...
Source: Virchows Archiv - April 15, 2020 Category: Pathology Source Type: research

How does intestinal-type intraductal papillary mucinous neoplasm emerge? CDX2 plays a critical role in the process of intestinal differentiation and progression
AbstractIntestinal-type intraductal papillary mucinous neoplasm (IPMN) of the pancreas is clinicopathologically distinctive. Our research aimed to elucidate the molecular mechanism of the development and progression of the intestinal-type IPMN. In 60 intestinal-type IPMN specimens, histological transitions from gastric-type epithelia to intestinal-type epithelia were observed in 48 cases (80%). CDX2/MUC2/alcian blue triple staining indicated that CDX2 appeared to precede MUC2 expression and subsequent alcian blue-positive mucin production. Expression of p21 and Ki-67 seemed to be accelerated by CDX2 expression (p =&...
Source: Virchows Archiv - April 15, 2020 Category: Pathology Source Type: research

Memento for interprofessional learning
AbstractThe vast increase of technical, diagnostic, and treatment possibilities and deepened understanding of molecular biology has revolutionized diagnosis and treatment of cancer and thus has great impact on pathology. Different professionals are responsible for proper evaluation of the results and their translating into an accurate diagnosis and appropriate treatment. Next to expertise, a close interaction between clinical molecular biologists, pathologists, and oncologists is required; it is crucial that these professionals speak “the same language.” Key to this is communication skills and creating possibil...
Source: Virchows Archiv - April 8, 2020 Category: Pathology Source Type: research

Immunohistochemical expression of Napsin A in normal human fetal lungs at different gestational ages and in acquired and congenital pathological pulmonary conditions
In conclusion, this study demonstrates that Napsin A is produced early in fetal life, and that its production is increased in many diseases, presumably in an effort to r emedy functional pulmonary failure. (Source: Virchows Archiv)
Source: Virchows Archiv - April 8, 2020 Category: Pathology Source Type: research

Autopsy in adults with congenital heart disease (ACHD)
AbstractThe adult congenital heart diseases (ACHD) population is exceeding the pediatric congenital heart diseases (CHD) population and is progressively expanding each year, representing more than 90% of patients with CHD. Of these, about 75% have undergone surgical and/or percutaneous intervention for palliation or correction. Autopsy can be a very challenging procedure in ACHD patients. The approach and protocol to be used may vary depending on whether the pathologists are facing native disease without surgical or percutaneous interventions, but with various degrees of cardiac remodeling, or previously palliated or corre...
Source: Virchows Archiv - April 7, 2020 Category: Pathology Source Type: research

Poorly differentiated thyroid carcinoma with pleomorphic giant cells —a case report
We described the case of a 43-year-old woman with a 34-mm nodule in her thyroid right lobe. Microscopic examination revealed an encapsulated tumour with a main solid growth pattern and extensive capsular invasion. Multiple images of angioinvasion were observed. There was neither necrosis nor inflammation. Most of the tumour cells were medium-sized and intermingled with pleomorphic giant tumour cells with bizarre features. The immunoprofile (keratins +, TTF1+, Pax 8+) proved their thyroid origin. By NGS, no molecular alteration was identified. The patient was treated by surgery and radioiodine therapy and she has no recurre...
Source: Virchows Archiv - April 1, 2020 Category: Pathology Source Type: research

Tumor infiltrative growth pattern correlates with the immune microenvironment and is an independent factor for lymph node metastasis and prognosis in stage T1 esophageal squamous cell carcinoma
In conclusion, INFc is an independent risk factor for lymph node metastasis and an independent inferior prognostic factor for stage T1 ESCC. Furthermore, INFc is associated with immunosuppression, and the combination of the INF and TILs is useful for the risk stratification of prognosis. (Source: Virchows Archiv)
Source: Virchows Archiv - March 30, 2020 Category: Pathology Source Type: research

MAML2 rearrangement as a useful diagnostic marker discriminating between Warthin tumour and Warthin-like mucoepidermoid carcinoma
AbstractWarthin tumour is the second most common benign neoplasm of salivary glands. Despite its relatively characteristic histology, it may sometimes mimic other lesions. Here, we report two female non-smoker patients diagnosed with low-grade mucoepidermoid carcinoma with oncocytic epithelium and prominent lymphoid (Warthin-like) stroma and with molecularly confirmedMAML2 rearrangement. In addition, we screened a consecutive series of 114 Warthin tumour cases by means ofMAML2 break apart fluorescence in situ hybridization to assess its value in differential diagnosis.MAML2 rearrangement was detected in both mucoepidermoid...
Source: Virchows Archiv - March 28, 2020 Category: Pathology Source Type: research

Primary biliary adenosarcoma of the liver —a special and new entity
AbstractPrimary sarcoma of the liver (PSL) is a rare entity accounting for less than 1% of all liver cancers, with unknown aetiology. Several subtypes have been recognized, with histology playing an essential role in patient tailoring and management. We are about to report a case of an unusual PSL, with peculiar morphologic and immunohistochemical properties. A 65-year-old female with a multicystic hepatic lesion underwent surgery due to spontaneous rupture. Pathology revealed dilated bile duct-like structures lined by benign cuboidal/columnar epithelium surrounded by neoplastic proliferation of spindle and epithelioid cel...
Source: Virchows Archiv - March 26, 2020 Category: Pathology Source Type: research

In this issue
(Source: Virchows Archiv)
Source: Virchows Archiv - March 26, 2020 Category: Pathology Source Type: research

Frozen section diagnosis of gastrointestinal poorly cohesive and signet-ring cell adenocarcinoma: useful morphologic features to avoid misdiagnosis
AbstractFrozen section examination of adenocarcinomas with poorly cohesive growth, including signet-ring cell carcinoma, is challenging. Due to their diffuse morphology, the tumor cells may be indistinct and difficult to distinguish from inflammatory or stromal cells. Misdiagnosis may result in significant adverse clinical outcome. We performed a detailed retrospective analysis of such cases to identify features that are helpful to avoid misdiagnosis at the time of frozen section. We reviewed the original frozen section slides from 50 patients with poorly cohesive carcinoma (PCC) including 32 with positive and 18 with nega...
Source: Virchows Archiv - March 25, 2020 Category: Pathology Source Type: research

Comparative genomic profiling of glandular bladder tumours
AbstractPrimary glandular bladder tumours (bladder adenocarcinoma [BAC], urachal adenocarcinoma [UAC], urothelial carcinoma with glandular differentiation [UCg]) are rare malignancies with histological resemblance to colorectal adenocarcinoma (CORAD) in the majority of this subgroup. Definite case numbers are very low, molecular data are limited and the pathogenesis remains poorly understood. Therefore, this study was designed to complement current knowledge by in depth analysis of BAC (n = 12), UAC (n = 13), UCg (n = 11) and non-invasive glandular lesions (n = 19). I...
Source: Virchows Archiv - March 20, 2020 Category: Pathology Source Type: research

Prominent entrapment of respiratory epithelium in primary and metastatic intrapulmonary non-epithelial neoplasms: a frequent morphological pattern closely mimicking adenofibroma and other biphasic pulmonary lesions
This study highlights frequent respiratory epithelium entrapment in diverse non-epithelial lung tumors, both primary and metastatic. Recognition of this finding and use of adjunct IHC combined with clinical history should help to avoid misinterpretation as primary pulmonary biphasic neoplasm or as harmless adenofibroma. The vast majority of morphologically defined lung adenofibromas represent adenofibroma-like variants of histogenetically diverse entities so that a diagnosis of adenofibroma should be rendered only very rest rictively and then as a diagnosis by exclusion. (Source: Virchows Archiv)
Source: Virchows Archiv - March 19, 2020 Category: Pathology Source Type: research

Pleuropulmonary blastoma type I might arise in congenital pulmonary airway malformation type 4 by acquiring a Dicer 1 mutation
AbstractCongenital pulmonary airway malformation (CPAM) occurs most commonly in infants. It is divided into 5 types. The most common types 1 and 2 are cystic, type 0 presents as bronchial buds without alveolar tissue, most likely corresponding to alveolar dysgenesis, while type 3 is composed of branching bronchioles and appears as a solid lesion. A defect in the epithelial-mesenchymal crosstalk might be the underlying mechanism for all. Type 4 is a peripheral cystic lesion with a thin cyst wall covered by pneumocytes. CPAM 4 has been mixed up with pleuropulmonary blastoma (PPB) type I and some authors question its existenc...
Source: Virchows Archiv - March 19, 2020 Category: Pathology Source Type: research