Correction to: The effect of the peritoneal tumor microenvironment on invasion of peritoneal metastases of high-grade serous ovarian cancer and the impact of neoadjuvant chemotherapy
A correction to this paper has been published: (Source: Virchows Archiv)
Source: Virchows Archiv - December 2, 2020 Category: Pathology Source Type: research

Myopericytoma arising from myopericytosis —a hitherto unrecognized entity within the lung
AbstractTwo cases of myopericytosis combined with pericytoma originating within the lung are reported. These are rare pulmonary tumors. The differential diagnosis for hemangiopericytoma and pericytic tumors with glomus elements is discussed. Both myopericytic lesions mimic other lesions, which are more commonly seen in the lung. Based on the expression of vascular growth factor receptors 2 and 3, an antiangiogenic therapy was suggested for the patient with the myopericytoma. A treatment with an angiogenesis inhibitor resulted in a regression of the tumor, but not the precursor lesion. Probably a more specific therapy using...
Source: Virchows Archiv - November 26, 2020 Category: Pathology Source Type: research

ALK alterations in salivary gland carcinomas
AbstractSalivary gland carcinomas represent a heterogeneous group of poorly characterized head and neck tumors. The purpose of this study was to evaluateALK gene and protein aberrations in a large, well-characterized cohort of these tumors. A total of 182 salivary gland carcinomas were tested for anaplastic lymphoma kinase (ALK) positivity by immunohistochemistry (IHC) using the cut-off of 10% positive cells. ALK positive tumors were subjected to FISH analysis and followed by hybrid capture –based next generation sequencing (NGS). Of the 182 tumors, 8 were ALK positive by IHC. Further analysis using hybrid capture NG...
Source: Virchows Archiv - November 25, 2020 Category: Pathology Source Type: research

(Source: Virchows Archiv)
Source: Virchows Archiv - November 25, 2020 Category: Pathology Source Type: research

Correlation of PD-L1 expression with immunohistochemically determined molecular profile in endometrial carcinomas
AbstractEndometrial carcinoma programmed death-ligand 1 (PD-L1) expression in tumor cells (TCs) and tumor-associated inflammatory cells (ICs) have recently been reported in several studies which vary in terms of their cohort size, design, and methodology. We aimed to assess PD-L1 staining in endometrial carcinomas and correlate this with clinical and pathological factors and PTEN, ARID1A, p53, and MMR protein expression. PD-L1 immunohistochemistry was performed on whole tissue sections of all tumor blocks of 59 consecutive unselected endometrial carcinomas between November 2018 and September 2019. TC and IC PD-L1 positivit...
Source: Virchows Archiv - November 24, 2020 Category: Pathology Source Type: research

CD68- and CD163-positive tumor-associated macrophages in triple negative cancer of the breast
AbstractTumor-associated macrophages (TAMs) have recently been reported as an important factor in tumor growth and the progression of cancer. The prognostic significance of localizations and densities of TAMs in triple negative cancer (TNC) of the breast is not well understood. The aim of this study was to assess the localizations and densities of the TAMs subtype in TNC and examine their clinicopathological features. The study was based on 107 TNC cases operated on at Dokkyo Medical University Hospital using the pan-macrophage marker CD68 and the M2 macrophage marker CD163 in the tumor stroma (TS) and tumor nest (TN), res...
Source: Virchows Archiv - November 24, 2020 Category: Pathology Source Type: research

A combined histo-score based on tumor differentiation and lymphocytic infiltrate is a robust prognostic marker for mobile tongue cancer
This study shows that a histo-score combining tumor differentiation and lymphocytic infiltration should be given special consideration in treatment planning. Our results also illustrate that many variables can be scored with fewer options than previously suggested to increase their reproducibility, and still maintain their prognostic value. (Source: Virchows Archiv)
Source: Virchows Archiv - November 24, 2020 Category: Pathology Source Type: research

Causes behind error rates for predictive biomarker testing: the utility of sending post-EQA surveys
AbstractExternal quality assessment (EQA) schemes assess the performance of predictive biomarker testing in lung and colorectal cancer and have previously demonstrated variable error rates. No information is currently available on the underlying causes of incorrect EQA results in the laboratories. Participants in EQA schemes by the European Society of Pathology between 2014 and 2018 for lung and colorectal cancer were contacted to complete a survey if they had at least one analysis error or test failure in the provided cases. Of the 791 surveys that were sent, 325 were completed including data from 185 unique laboratories ...
Source: Virchows Archiv - November 23, 2020 Category: Pathology Source Type: research

Periostin as a key molecule defining desmoplastic environment in colorectal cancer
AbstractCategorizing desmoplastic reaction (DR) based on the histological findings of cancer-associated fibroblasts is shown to be a promising novel method to predict prognosis of patients with colorectal cancer (CRC). Periostin (POSTN) in cancer-associated stroma is reportedly associated with poor clinical outcomes. Immunohistochemical staining with an anti-POSTN antibody was performed in 73 patients with pStage III CRC (cohort 1). In addition, to evaluate mRNA and protein expression levels of POSTN, we analyzed paired normal and invasive cancer frozen specimens by quantitative real-time polymerase chain reaction and west...
Source: Virchows Archiv - November 19, 2020 Category: Pathology Source Type: research

Correction to: A case of primary distal-type epithelioid sarcoma of the lumbar vertebra with a review of literature
A correction to this paper has been published: (Source: Virchows Archiv)
Source: Virchows Archiv - November 18, 2020 Category: Pathology Source Type: research

In this issue
(Source: Virchows Archiv)
Source: Virchows Archiv - November 17, 2020 Category: Pathology Source Type: research

Identification of immune-related genes with prognostic significance in the microenvironment of cutaneous melanoma
In this study, cutaneous melanoma –associated TME was systematically analyzed using the ESTIMATE algorithm based on the gene transcriptome data obtained from the TCGA database. Totally, 471 patients were included and 553 TME-related genes were screened. Afterwards, a 3-gene signature–based model (CLEC4A, GBP4, KIR2DL4) was const ructed via univariate Cox, LASSO, and multivariate Cox regression analyses. To validate the validity of this model, ROC analysis was conducted, and the model was further validated to be an independent prognostic biomarker through univariate and multivariate regression analyses. Finally,...
Source: Virchows Archiv - November 12, 2020 Category: Pathology Source Type: research

The impact of pre-analytical parameters on class II biomarkers by immunohistochemistry: concordance across four tissue processing protocols
In this study, we aimed to study the impact of tissue processing on biomarkers (including ER, PR, HER2, mismatch repair (MMR) proteins, BRAF V600E, and PD-L1) in a large prospective cohort of 109 tumors. We found that ER and MMR were not impacted; PR, HER2, and BRAF V600E were minimally affected; and PD-L1 regardless of the antibody clone was strongly influenced by a combination of tissue processing procedures and intratumoral heterogeneity. Our findings suggest that validation of pre-analytical parameters, such as tissue processing, is important for certain class II biomarkers, in particular PD-L1 IHC. (Source: Virchows Archiv)
Source: Virchows Archiv - November 11, 2020 Category: Pathology Source Type: research

Multi-center real-world comparison of the fully automated Idylla ™ microsatellite instability assay with routine molecular methods and immunohistochemistry on formalin-fixed paraffin-embedded tissue of colorectal cancer
In conclusion, lower failure rates and high concordance levels were found between the Idylla™ MSI Assay and routine tests. (Source: Virchows Archiv)
Source: Virchows Archiv - November 10, 2020 Category: Pathology Source Type: research

Nodal EBV-positive polymorphic B cell lymphoproliferative disorder with plasma cell differentiation: clinicopathological analysis of five cases
AbstractPlasma cell differentiation (PCD) is frequently observed in some entities of non-Hodgkin B cell lymphoma, including both low-grade and high-grade lymphomas. However, except for plasmablastic lymphoma and primary effusion lymphoma, EBV+ B cell lymphoproliferative disorder (LPD) with PCD has not been well addressed due to its rarity. We clinicopathologically examined five cases of nodal EBV+ polymorphic B cell LPD with PCD (PBLPD-PCD) initially diagnosed as polymorphic EBV+ diffuse large B cell lymphoma, not otherwise specified (DLBCL-NOS) with PCD (n = 3) and methotrexate-associated B cell LPD (MTX-ass...
Source: Virchows Archiv - November 9, 2020 Category: Pathology Source Type: research

Molecular approach to the classification of chronic fibrosing lung disease —there and back again
AbstractChronic diffuse parenchymal lung disease (DPLD) is an umbrella term for a very heterogeneous group of lung diseases. Over the last decades, clinical, radiological and histopathological criteria have been established to define and separate these entities. More recently the clinical utility of this approach has been challenged as a unifying concept of pathophysiological mechanisms and a shared response to therapy across the disease spectrum have been described. In this review, we discuss molecular motifs for subtyping and the prediction of prognosis focusing on genetics and markers found in the blood, lavage and tiss...
Source: Virchows Archiv - November 9, 2020 Category: Pathology Source Type: research

Atypical nodular fasciitis with a novel PAFAH1B1-USP6 fusion in a 22-month-old boy
AbstractNodular fasciitis (NF) rarely occurs in infants aged
Source: Virchows Archiv - November 8, 2020 Category: Pathology Source Type: research

Clinicopathologic and molecular features of six cases of phosphaturic mesenchymal tumor
AbstractPhosphaturic mesenchymal tumors (PMT) are rare neoplasms characterized by secretion of FGF23, resulting in renal phosphate wasting and osteomalacia. This tumor-induced osteomalacia (TIO) is cured by complete resection; thus, diagnosis is important, particularly on biopsy. Although PMT have a classic histologic appearance of bland spindled cells with conspicuous vascular network and characteristic smudgy basophilic matrix, there is a broad histologic spectrum and variant histologic patterns can make recognition difficult. Recent studies have demonstratedFN1-FGFR1 andFN1-FGF1 gene fusions in PMT; however, approximate...
Source: Virchows Archiv - November 5, 2020 Category: Pathology Source Type: research

Spiradenoma of the breast: a rare diagnostic pitfall in the evaluation of solid-basaloid breast lesions with a dual cell population
We describe an instructive case of breast spiradenoma resembling adenoid cystic carcinoma (AdCC). A 71-year-old woman underwent excisional biopsy of a breast mass after a conclusive diagnosis was unable to be obtained from core needle biopsy showing an AdCC-like pattern. Histopathologically, the lesion demonstrated solid and cribriform foci comprising basaloid cells, luminal cells, and eosinophilic hyaline material, reminiscent of solid-basaloid AdCC, alongside convoluted lumens, stromal edema, lymphocytic infiltration, and c-kit negativity. On molecular analysis, neitherMYB fusion genes norCYLD gene abnormalities were ide...
Source: Virchows Archiv - November 5, 2020 Category: Pathology Source Type: research

Myoid gonadal tumor. Case series, systematic review, and Bayesian analysis
In conclusion, we foresee the use of th is statistical approach in pathology: our analysis allowed a more precise description of this rare entity. (Source: Virchows Archiv)
Source: Virchows Archiv - November 2, 2020 Category: Pathology Source Type: research

Breast ductal Carcinoma in situ associated with microinvasion induces immunological response and predicts ipsilateral invasive recurrence
This study investigated the potential influence of Mi on disease-free survival (DFS) and assessed its correlations with clinicopathological parameters, prognosis, molecular, and immune markers. CD4, CD8, forkhead box P3 (FOXP3), CD68, CD163, programmed cell death protein 1 (PD-1), and its ligand (PD-L1) expression in pure DCIS and DCIS-Mi, from a cohort of 198 patients, were determined by immunohistochemistry. DFS, clinicopathological parameters, immune markers, and biomarker expression were correlated with presence of Mi. Twelve out of 198 DCIS cases were associated with Mi. DCIS-Mi was significantly linked with ipsilater...
Source: Virchows Archiv - November 2, 2020 Category: Pathology Source Type: research

Tumor-infiltrating lymphocyte abundance and programmed death-ligand 1 expression in metaplastic breast carcinoma: implications for distinct immune microenvironments in different metaplastic components
AbstractBoth stromal tumor –infiltrating lymphocytes (sTILs) and programmed death-ligand 1 (PD-L1) affect responses to immunotherapy; however, the extent of sTIL and PD-L1 expression within various metaplastic components in metaplastic breast carcinoma (MBC), which are critical for the characterization of immune microenviro nments, remains unreported. We profiled sTIL infiltration and PD-L1 expression in different metaplastic components of specimens from 82 MBC patients. The overall positivity for high or intermediate (H/I) sTIL, immune cell-PD-L1 (IcPD-L1), and tumor cell-PD-L1 (TcPD-L1) was 34.1%, 47.6%, and 17.1%,...
Source: Virchows Archiv - October 21, 2020 Category: Pathology Source Type: research

Expression profiles of metallothionein-I/II and megalin/LRP-2 in uterine cervical squamous lesions
AbstractMetallothioneins (MTs) are phylogenetically old cysteine-rich proteins, which are implicated in a variety of physiological and pathological processes. Their growth-regulating, anti-apoptotic and anti-inflammatory functions have been attributed not only to intracellular free radical scavenging and to zinc and copper regulation but also to the ability of secreted MT to bind on surface lipoprotein receptor-megalin/LRP2, which enables the endocytosis of MT-I/II and a wide range of other functionally distinct ligands. In the present study, we analysed the expression pattern of both proteins in 55 cases of premalignant t...
Source: Virchows Archiv - October 21, 2020 Category: Pathology Source Type: research

Sclerosing epithelioid fibrosarcoma of bone: morphological, immunophenotypical, and molecular findings of 9 cases
AbstractPrimary sclerosing epithelioid fibrosarcoma (SEF) of bone is a rare and scarcely reported neoplasm. We document clinicopathological and molecular features of 9 additional cases. Five males and 4 females had a mean age of 39 years (14 –71 years). Most tumors affected flat/irregular bones; only 3 cases involved a long bone. By radiology, it has characteristic radiographic features of a predominantly lytic expansile lesion with a sclerotic rim. Referring diagnoses were SEF (n = 2), low-grade osteosarcoma (n = 2), chondrosarcoma (n = 1), and chondromyxoid fibroma (n = 1). Histologically, five cases revealed class...
Source: Virchows Archiv - October 21, 2020 Category: Pathology Source Type: research

In this issue
(Source: Virchows Archiv)
Source: Virchows Archiv - October 15, 2020 Category: Pathology Source Type: research

EGFR and BRAF mutations in inverted sinonasal papilloma — a more complex landscape?
AbstractInverted (Schneiderian) sinonasal papilloma (ISP) is a neoplasm derived from mucosa of the sinonasal tract characterized by local aggressive growth, a tendency to recur and an association with sinonasal carcinoma. The etiology of ISP remains unclear. Recently, identical mutations in exons 19 and 20 of the oncogeneEGFR were reported in ISP and ISP-associated sinonasal carcinoma. Nevertheless, it remains unclear whether recurring ISPs show identicalEGFR mutations at different time points or whether these mutations are identical throughout the respective ISP sample. We used Sanger sequencing to test 60 formalin-fixed ...
Source: Virchows Archiv - October 13, 2020 Category: Pathology Source Type: research

Biomarker testing in oncology – Requirements for organizing external quality assessment programs to improve the performance of laboratory testing: revision of an expert opinion paper on behalf of IQNPath ABSL
AbstractIn personalized medicine, predictive biomarker testing is the basis for an appropriate choice of therapy for patients with cancer. An important tool for laboratories to ensure accurate results is participation in external quality assurance (EQA) programs. Several providers offer predictive EQA programs for different cancer types, test methods, and sample types. In 2013, a guideline was published on the requirements for organizing high-quality EQA programs in molecular pathology. Now, after six years, steps were taken to further harmonize these EQA programs as an initiative by IQNPath ABSL, an umbrella organization ...
Source: Virchows Archiv - October 13, 2020 Category: Pathology Source Type: research

Co-existence of lung carcinoma metastasis and enchondroma in the femur of a patient with Ollier disease
We report a case of lung squamous cell carcinoma metastasized to an enchondroma in the femur of a patient with Ollier disease. A 60-year-old female had a history of a poorly differentiated squamous cell carcinoma of the lung. She underwent a video-assisted thoracoscopic lobectomy, and a follow-up MRI scan showed three lesions in the left distal femur and proximal tibia, which were initially interpreted as metastasis on radiology. Resection of the left proximal tibial lesion was performed, and the pathological findings were consistent with enchondroma with no evidence of metastasis. Subsequent curettage of lesions in the di...
Source: Virchows Archiv - October 13, 2020 Category: Pathology Source Type: research

Expanding spectrum of “spitzoid” lesions: a small series of 4 cases with MAP2K1 mutations
We report four cases of melanocytic tumors with spitzoid features in which aMAP2K1 mutation was detected. The lesions did not show a single distinct phenotype and ranged from benign to malignant. Two cases resembled desmoplastic Spitz nevi. Based on the combination of morphological, immunohistochemical, and molecular findings, one case was classified as benign, one as probably benign, possibly intermediate low-grade (MELTUMP —melanocytic tumor of unknown malignant potential), one case was classified as intermediate (MELTUMP), and one case was considered a superficial spreading melanoma with spitzoid features. Based o...
Source: Virchows Archiv - October 11, 2020 Category: Pathology Source Type: research

TAp63 and ΔNp63 (p40) in prostate adenocarcinomas: ΔNp63 associates with a basal-like cancer stem cell population but not with metastasis
AbstractLike other malignancies, prostate tumors are thought to contain cancer stem-like cells (CSCs) that are responsible for growth, metastasis, and therapy resistance. ΔNp63 (also called p40) is a regulator of normal prostate stem/progenitor cell activities and a marker of normal basal epithelial cells. The levels of ΔNp63 are reduced in prostate adenocarcinomas, although there is also evidence that ΔNp63 is involved in CSC regulation and drives metastasis to t he bone. We studied metastatic deposits of prostate cancers with isoform-specific ΔNp63 and TAp63 antibodies. We identified p63-positive ...
Source: Virchows Archiv - October 10, 2020 Category: Pathology Source Type: research

Duodenal intraepithelial lymphocytosis in Helicobacter pylori gastritis: comparison before and after treatment
This study confirms previous reports of increased duodenal intraepithelial lymphocytes in patients with concurrentHelicobacter pylori gastritis. Intraepithelial lymphocyte counts of>  15 per villus or>  10 per 100 enterocytes were predictive of infection. Duodenal lymphocytosis decreases significantly after successful eradication therapy but remains elevated when treatment fails. (Source: Virchows Archiv)
Source: Virchows Archiv - October 5, 2020 Category: Pathology Source Type: research

Insulinoma-associated protein 1 (INSM1) expression in breast carcinomas with neuroendocrine morphologies: application and future prospective
AbstractHerein, we describe the first cases of neuroendocrine (NE) phenotype mammary carcinomas in which the NE nature of the tumors was confirmed only by insulinoma-associated protein 1 (INSM1), without expressions of traditional “gold standard” NE indicators. This is also the first analysis to use INSM1, a promising antibody with high sensitivity and specificity, in the field of breast oncology. Three patients were, respectively, 42-, 58-, and 64-year-old Japanese women with breast tumors showing characteristic NE morph ologies. Immunohistochemically, these malignancies revealed diffuse nuclear expressions of...
Source: Virchows Archiv - October 5, 2020 Category: Pathology Source Type: research

ALK-positive histiocytosis associated with chronic lymphocytic leukaemia/small lymphocytic lymphoma: a multitarget response under ibrutinib
This report extends the spectrum of composite hematolymphoid neoplasms and shows thatALK rearrangement should be considered in all histiocytosis subtypes. Moreover, both tumours eradication under ibrutinib suggests that BTK inhibitors may also be effective in histiocytic neoplasms. (Source: Virchows Archiv)
Source: Virchows Archiv - October 2, 2020 Category: Pathology Source Type: research

Histological classification of mucinous ovarian tumors: inter-observer reproducibility, clinical relevance, and role of genetic biomarkers
AbstractThe morphological distinction between the various types of mucinous ovarian tumors has major prognostic implications but may be challenging. The aims of our study were to describe inter-observer reproducibility in the morphological diagnosis of mucinous ovarian tumors, to evaluate the clinical relevance of possible diagnostic discrepancies, and to identify molecular abnormalities correlated with the histological type. Seventy-nine ovarian mucinous borderline tumors (MOB) and either expansile or infiltrative carcinomas (MOC) were independently reviewed by two gynecological pathologists. Molecular analysis was perfor...
Source: Virchows Archiv - October 2, 2020 Category: Pathology Source Type: research

Clear cell sarcoma-like/malignant gastrointestinal neuroectodermal tumor of the tongue: a clinicopathologic and molecular case report
AbstractMalignant gastrointestinal neuroectodermal tumor (M-GNET) and clear cell sarcoma (CCS) of soft tissue represent closely related, extremely rare, malignant mesenchymal neoplasm of uncertain differentiation. Both entities are characterized genetically by the same molecular alterations represented by the presence ofEWSR1-ATF1 and, more rarely,EWSR1-CREB1 fusion genes. The latter translocation seems to be more represented in M-GNET that, despite significant morphologic overlap with CCS, tends to lack overt features of melanocytic differentiation. Most M-GNET occur in the lower gastrointestinal tract, whereas occurrence...
Source: Virchows Archiv - October 1, 2020 Category: Pathology Source Type: research

Evidence of a common cell origin in a case of pancreatic mixed intraductal papillary mucinous neoplasm –neuroendocrine tumor
In this report, a pancreatic intraductal papillary mucinous neoplasm (IPMN) plus a NET is described, and the molecular investigations showed the presence in both populations of the same KRAS, GNAS, and CDKN2A mutations and the amplification of the CCND1 gene. These data prove clonality and support a common origin of both components, therefore confirming the true mixed nature. For this reason, mixed neuroendocrine-exocrine neoplasms, in which the exocrine component is represented by a glandular precursor lesion (adenoma/IPMN) only, should be included into the MiNEN family. (Source: Virchows Archiv)
Source: Virchows Archiv - September 30, 2020 Category: Pathology Source Type: research

Fibrotic progression and radiologic correlation in matched lung samples from COVID-19 post-mortems
AbstractData on the pathology of COVID-19 are scarce; available studies show diffuse alveolar damage; however, there is scarce information on the chronologic evolution of COVID-19 lung lesions. The primary aim of the study is to describe the chronology of lung pathologic changes in COVID-19 by using a post-mortem transbronchial lung cryobiopsy approach. Our secondary aim is to correlate the histologic findings with computed tomography patterns. SARS-CoV-2-positive patients, who died while intubated and mechanically ventilated, were enrolled. The procedure was performed 30 min after death, and all lung lobes sampled. Histop...
Source: Virchows Archiv - September 27, 2020 Category: Pathology Source Type: research

Human kidney graft survival correlates with structural parameters in baseline biopsies: a quantitative observational cohort study with more than 14 years ’ follow-up
AbstractThis prospective cohort study evaluates associations between structural and ultrastructural parameters in baseline biopsies from human kidney transplants and long-term graft survival after more than 14 years ’ follow-up. Baseline kidney graft biopsies were obtained prospectively from 54 consecutive patients receiving a kidney transplant at a single institution. Quantitative measurements were performed on the baseline biopsies by computer-assisted light microscopy and electron microscopy. Stereology-ba sed techniques estimated the fraction of interstitial tissue, the volume of glomeruli, mesangial fraction, an...
Source: Virchows Archiv - September 27, 2020 Category: Pathology Source Type: research

Artificial intelligence to detect MYC translocation in slides of diffuse large B-cell lymphoma
AbstractIn patients with suspected lymphoma, the tissue biopsy provides lymphoma confirmation, classification, and prognostic factors, including genetic changes. We developed a deep learning algorithm to detect MYC rearrangement in scanned histological slides of diffuse large B-cell lymphoma. The H&E-stained slides of 287 cases from 11 hospitals were used for training and evaluation. The overall sensitivity to detect MYC rearrangement was 0.93 and the specificity 0.52, showing that prediction of MYC translocation based on morphology alone was possible in 93% of MYC-rearranged cases. This would allow a simple and fast p...
Source: Virchows Archiv - September 25, 2020 Category: Pathology Source Type: research

The use of digital microscopy as a teaching method for human pathology: a systematic review
This study presents the efficacy of DM for human pathology education, although the high heterogenei ty of the included articles did not permit outlining a specific method of performance evaluation. (Source: Virchows Archiv)
Source: Virchows Archiv - September 22, 2020 Category: Pathology Source Type: research

Stromal categorization in early oral tongue cancer
In conclusion, categorization of the stroma based on the amount of stroma and its infiltrating lymphocytes shows clinical relevance in early OTSCC superior to categorization based on the maturity of stroma. (Source: Virchows Archiv)
Source: Virchows Archiv - September 20, 2020 Category: Pathology Source Type: research

Association of tumor-infiltrating T lymphocytes with intestinal-type gastric cancer molecular subtypes and outcome
AbstractWhile host immune response is likely to be important for the prognosis of gastric cancer patients, detailed information on the T lymphocyte infiltration in different gastric cancer subtypes is lacking. Here, we studied the presence of CD3, CD8, and FOXP3 (Forkhead box p3) expressing T lymphocytes in a retrospective cohort of 190 intestinal gastric and gastroesophageal adenocarcinomas. The cancers represented four distinct molecular subtypes: Epstein-Barr virus –positive (EBV+), mismatch-repair-deficient (MMR-D), aberrant TP53, and the “other” subtype. The absolute numbers of CD3+, CD8+, and FOXP3+...
Source: Virchows Archiv - September 20, 2020 Category: Pathology Source Type: research

Elastosis in ER α-positive male breast cancer
AbstractIn female breast cancer (BC), elastosis is strongly related to estrogen receptor alpha (ER α) expression. Male breast cancers almost invariably express ERα; so, the aim of this study was to investigate elastosis frequency in invasive male BC as well as clinicopathological correlations, in comparison with females. A total of 177 male BC cases and 135 female BC cases were included, all ER α-positive and invasive carcinoma of no special type. Elastosis on H&E-stained slides was scored in a four-tiered system as elastosis grade (EG) 0 (no elastosis) to EG3 (high amount of elastosis). EG scores in ...
Source: Virchows Archiv - September 14, 2020 Category: Pathology Source Type: research

Primary glomus tumour of the pituitary gland: diagnostic challenges of a rare and potentially aggressive neoplasm
We describe two cases of clinically aggressive primary glomus tumour of the pituitary gland. The lesions occurred in a 63-year-old male and a 30-year-old female who presented with headache, blurred vision and hypopituitarism. Neuroimaging demonstrated large sellar and suprasellar tumours invading the surrounding structures. Histologically, the lesions were characterised by angiocentric sheets and nests of atypical cells that expressed vimentin, smooth muscle actin and CD34. Perivascular deposition of collagen IV was also a feature. Case 2 expressed synaptophysin. INI-1 (SMARCB1) expression was preserved. Both lesions were ...
Source: Virchows Archiv - September 11, 2020 Category: Pathology Source Type: research

Temporal heterogeneity of placental segmental fetal vascular malperfusion: timing but not etiopathogenesis
AbstractClinicopathologic correlations of segmental villous avascularity and other histological lesions of segmental fetal vascular malperfusion (SFVM) were analyzed retrospectively to determine whether lesions of various durations reflect different etiopathogeneses. The frequencies of 25 independent clinical and 43 placental phenotypes were statistically compared by ANOVA or Chi-square among 3 groups containing a total of 378 placentas with SFVM: group 1 contained 44 cases of recent SFVM (endothelial fragmentation, villous hypovascularity by CD34 immunostain, and/or stromal vascular karyorrhexis); group 2 contained 264 ca...
Source: Virchows Archiv - September 11, 2020 Category: Pathology Source Type: research

Characterization of a distinct low-grade oncocytic renal tumor (CD117-negative and cytokeratin 7-positive) based on a tertiary oncology center experience: the new evidence from China
We described a group of low-grade oncocytic renal tumors identified retrospectively in a large tertiary cancer center, which was probably the first report originated from China or even Asia in the English literature so far. These tumors demonstra ted eosinophilic cytoplasm and low-grade appearing nuclei with a “CD117 negative/CK7 positive” immunoprofile. The incidence rate was about 3.7% of the oncocytic renal tumors and 0.18% of all the renal tumors that were received in our lab during the four-year period. It is necessary to separate t his group of tumors by its characteristic morphologic and immunophenotypic...
Source: Virchows Archiv - September 11, 2020 Category: Pathology Source Type: research

PD-L1 expression is associated with tumor infiltrating lymphocytes that predict response to NACT in squamous cell cervical cancer
AbstractCancer immunotherapy has significantly improved the management of many malignancies in recent years. Although cervical cancer is the second most common women ’s cancer in the world, there are still few information about the role of checkpoint inhibitors in this neoplasm, especially in the neoadjuvant setting. In the present study, we retrieved 38 consecutive patients with squamous cell cervical cancer who underwent platinum-based neoadjuvant chemothera py (NACT) followed by radical surgery. Pre-therapy biopsies were evaluated for the presence of tumor-infiltrating lymphocytes (TILs), including T (both cytotox...
Source: Virchows Archiv - September 10, 2020 Category: Pathology Source Type: research

Immunohistochemistry as a screening tool for NTRK gene fusions: results of a first Belgian ring trial
AbstractA Belgian ring trial for pan-TRK immunohistochemistry (IHC) staining was organised to harmonise pan-TRK IHC staining protocols and interpretation. As a reference method, the VENTANA pan-TRK Assay (clone EPR17341) on the Benchmark Ultra platform was selected. Six samples were selected: 2 negative, 2 fusion positive and 2 samples with wild-type endogenous TRK expression. Each participating laboratory stained the slides using their routine pan-TRK IHC and reported their results. In addition, they were asked to return one TRK-stained slide from each case. The coordinating lab evaluated these slides, compared them with ...
Source: Virchows Archiv - September 10, 2020 Category: Pathology Source Type: research

Increased number of pulmonary megakaryocytes in COVID-19 patients with diffuse alveolar damage: an autopsy study with clinical correlation and review of the literature
AbstractPulmonary megakaryocytes participate in the pathogenesis of lung damage, particularly in acute lung injury. Although megakaryocytes are not mentioned as a characteristic histologic finding associated to pulmonary injury, a few studies reveal that their number is increased in diffuse alveolar damage (DAD). In this autopsy study, we have observed a relevant number of pulmonary megakaryocytes in COVID-19 patients dying with acute lung injury (7.61 ± 5.59 megakaryocytes per 25 high-power fields vs. 1.14 ± 0.86 for the control group,p
Source: Virchows Archiv - September 10, 2020 Category: Pathology Source Type: research

In this issue
(Source: Virchows Archiv)
Source: Virchows Archiv - September 10, 2020 Category: Pathology Source Type: research