Expression of the potential therapeutic target claudin-18.2 is frequently decreased in gastric cancer: results from a large Caucasian cohort study
In conclusion, we demonstrate frequently decreased expression of CLDN18.2 in a GC cohort of appropriate size. Correlating CLD N18.2 expression with clinico-pathological patient characteristics reveals new linkages to αvβ5, EpEX, and lysozyme, which may pave the way for further investigations regarding the role of tight junction proteins in GC progression. Though CLDN18.2 continues to pose an attractive target candidate, we conclude that a rather low overall expression rate challenges its significance in advanced GC therapy and indicates the need for further investigations across different populations. (Source: Virchows Archiv)
Source: Virchows Archiv - July 22, 2019 Category: Pathology Source Type: research

SOX11 expression in a case of papillary thyroid carcinoma with fibromatosis/fasciitis-like stroma containing BRAF c.1799_1801delTGA and CTNNB1 c.133T & gt;C mutations
We describe a case of papillary thyroid carcinoma with fibromatosis/fasciitis-like stroma (PTC-FLS) that contained the rareBRAF c.1799_1801delTGA (p.V600_K601delinsE) mutation, which has not previously been reported in this tumour, as well as theCTNNB1 c.133T>C (p.S45P) mutation. We also report the novel observation that spindle cells of the mesenchymal component exhibit diffuse nuclear but not cytoplasmic expression of SOX11, whereas the malignant epithelial cells did not. This suggests that immunoreactivity for SOX11 can be an alternative diagnostic tool for evaluating cases of PTC-FLS where the nuclear expression of ...
Source: Virchows Archiv - July 20, 2019 Category: Pathology Source Type: research

Clonal evolution of chronic lymphocytic leukemia to Langerhans cell histiocytosis: a case report
AbstractThe traditional concept of unidirectional maturation of hematopoietic cells has been called into question due to the recognition of lineage plasticity, which is increasingly found also in the clonal evolution of hematopoietic and lymphoid malignancies. Here we present an unusual case of a patient withTP53-mutated chronic lymphocytic leukemia (CLL) treated with a PI3K δ inhibitor evolving to clonally related Langerhans cell histiocytosis (LCH) with acquiredBRAF V600E andSTK11 mutations and loss of expression of PAX-5 and other examined B cell markers. In indolent B cell lymphoma, transformation to a more aggre...
Source: Virchows Archiv - July 17, 2019 Category: Pathology Source Type: research

In this issue
(Source: Virchows Archiv)
Source: Virchows Archiv - July 15, 2019 Category: Pathology Source Type: research

Immunohistochemical assessment of basal and luminal markers in non-muscle invasive urothelial carcinoma of bladder
AbstractThe Cancer Genome Atlas project introduced genomic taxonomy of basal and luminal molecular subtypes in muscle invasive bladder cancer. Fewer studies have addressed the molecular classification in non-muscle invasive bladder cancer (NMIBC). Our aim is to assess the applicability of the proposed phenotypic classification for NMIBC. Three TMAs were constructed from 193 TURBT specimens of 60 bladder cancer patients treated at one of the authors ’ institutions (1998–2008). Follow-up data on recurrence, grade, or stage progression was obtained. Immunohistochemistry was performed using an automated Ventana Sys...
Source: Virchows Archiv - July 12, 2019 Category: Pathology Source Type: research

The spectrum of myocarditis: from pathology to the clinics
AbstractMyocarditis is an inflammatory disease of the myocardium, which may occur in isolation or as part of systemic infectious/immune/autoimmune conditions, characterized by vast aetiologic, clinical and histopathologic heterogeneity. The broad spectrum of myocarditis can be categorized according to the prevalent histopathologic pattern including lymphocytic, lympho-histiocytic, eosinophilic and neutrophilic forms, giant cell myocarditis and myocarditis with granulomata. Diverse histopathologic substrates generally reflect different aetiologies and pathogenetic mechanisms and may be critical to clinical decision-making. ...
Source: Virchows Archiv - July 11, 2019 Category: Pathology Source Type: research

Identification of barriers and facilitators in nationwide implementation of standardized structured reporting in pathology: a mixed method study
The objective of this study is to identify both barriers and facilitators that pathologists encounter in SSR, in order to develop tailored implementation tools to increase SSR usage. We used a mixed method design: a focus group interview helped to identify barriers and facilitators in SSR. The findings were classified into the following domains: innovation, individual professional, social setting, organization, and economic and political context. We used a web-based survey among Dutch pathologists to quantify the findings. Ten pathologists participated in the focus group interview, and 97 pathologists completed the survey....
Source: Virchows Archiv - July 3, 2019 Category: Pathology Source Type: research

Impact of delayed fixation and decalcification on PD-L1 expression: a comparison of two clones
AbstractThe bone is a frequent localization for lung non-small cell cancer metastasis; decalcification is required to permit tissue section. Pre-analytical conditions can influence the detection of immunohistochemical markers. The aim of our work is to evaluate PD-L1 expression in samples with delayed fixation and in decalcified tissue with chelating agent or acid at different time. Tumor-expressing PD-L1 and placental tissue were fixed at different times or decalcified with an acid decalcifier or EDTA for different durations. For 22C3 antibody, when tissues were decalcified with DC3, there was a significant decrease in th...
Source: Virchows Archiv - July 2, 2019 Category: Pathology Source Type: research

Multicentric analytical comparability study of programmed death-ligand 1 expression on tumor-infiltrating immune cells and tumor cells in urothelial bladder cancer using four clinically developed immunohistochemistry assays
We examined technical comparability and inter-reader agreement of four clinically developed PD-L1 assays in locally advanced disease. Archived, formalin-fixed, paraffin-embedded sections from 30 patients (73.3% cystectomies, 26.7% transurethral resections) were stained by PD-L1 immunohistochemistry using VENTANA SP142, VENTANA SP263, DAKO 22C3, and DAKO 28-8 at two sites per manufacturers ’ protocols and scored blinded at five sites for PD-L1 expression on IC (% per tumor area) and TC (%). Small, non-significant inter-assay differences were observed for IC. For TC, SP142 showed significantly lower staining percentage...
Source: Virchows Archiv - July 2, 2019 Category: Pathology Source Type: research

In this issue
(Source: Virchows Archiv)
Source: Virchows Archiv - July 2, 2019 Category: Pathology Source Type: research

Microfluidic-based immunohistochemistry for breast cancer diagnosis: a comparative clinical study
AbstractBreast cancer is a highly heterogeneous disease. The efficacy of tailored therapeutic strategies relies on the precise detection of diagnostic biomarkers by immunohistochemistry (IHC). Therefore, considering the increasing incidence of breast cancer cases, a concomitantly time-efficient and accurate diagnosis is clinically highly relevant. Microfluidics is a promising innovative technology in the field of tissue diagnostic, enabling for rapid, reliable, and automated immunostaining. We previously reported the microfluidic-based HER2 (human epidermal growth factor receptor 2) detection in breast carcinomas to greatl...
Source: Virchows Archiv - July 2, 2019 Category: Pathology Source Type: research

Polyomavirus JCPyV infrequently detectable in adenoid cystic carcinoma of the oral cavity and the airways
In conclusion, only JCPyV but not SV4 0, BKPyV, or HPV was found in ACC from the upper and lower airways. JCPyV copy numbers were low which might support its role as a “hit and run agent” in ACC carcinogenesis. (Source: Virchows Archiv)
Source: Virchows Archiv - July 1, 2019 Category: Pathology Source Type: research

ACTH-producing tumorlets and carcinoids of the lung: clinico-pathologic study of 63 cases and review of the literature
AbstractAdrenocorticotropic hormone (ACTH)-secreting lung carcinoids represent the principal cause of ectopic Cushing syndrome, but the prevalence of ACTH expression and the association between ACTH production and Cushing syndrome in lung carcinoids have scarcely been investigated. In addition, available information on the prognostic meaning of ACTH production is controversial. The aims of this multicentric retrospective study, also including a review of the literature, were to describe the clinico-pathologic features of ACTH-producing lung carcinoids, to assess recurrence and specific survival rates, and to evaluate poten...
Source: Virchows Archiv - July 1, 2019 Category: Pathology Source Type: research

Impact of delayed and prolonged fixation on the evaluation of immunohistochemical staining on lung carcinoma resection specimen
AbstractPre-analytical factors, such as fixation time, influence morphology of diagnostic and predictive immunohistochemical staining, which are increasingly used in the evaluation of lung cancer. Our aim was to investigate if variations in fixation time influence the outcome of immunohistochemical staining in lung cancer. From lung resections, specimen with tumor size bigger than 4  cm, 10 samples were obtained: 2 were put through the standard fixation protocol, 5 through the delayed, and 3 through the prolonged fixation protocol. After paraffin embedding, tissue microarrays (TMAs) were made. They were stained with 2...
Source: Virchows Archiv - July 1, 2019 Category: Pathology Source Type: research

Thirteen cases of pulmonary dirofilariasis in a single institution in Okinawa Island
AbstractPulmonary dirofilariasis is an infection caused byDirofilaria immitis, which is an endemic parasite in Japan. We experienced 13 surgical cases of pulmonary dirofilariasis in our hospital. Of the 13 patients, 61.5% were men. The responsible lesions were located in the right lung in all cases, and 76.9% of them were in the lower lobe. Histologically, 12 cases showed necrotic nodules with peripheral granuloma with worms inside the pulmonary artery. One case did not show a necrotic nodule but showed only thickening and hyalinization of the pulmonary artery wall with a degenerated worm inside. Eosinophils were found his...
Source: Virchows Archiv - June 28, 2019 Category: Pathology Source Type: research

Comparative genome-wide analysis of gastric adenocarcinomas with hyperplastic polyp components
AbstractGastric hyperplastic polyps are common and generally regarded as benign lesions, whereas gastric adenocarcinomas infrequently occur from gastric hyperplastic polyps. Although gastric hyperplastic polyps have received a lot of attention because of their association with malignant transformation, it remains unclear whether gastric hyperplastic polyps are neoplastic lesions that have sporadic genetic changes similar to colorectal hyperplastic polyps. We performed genome-wide analyses of two gastric adenocarcinomas with hyperplastic polyp components. The interface between “adenocarcinoma” and “hyperpl...
Source: Virchows Archiv - June 28, 2019 Category: Pathology Source Type: research

The molecular pathogenesis of penile carcinoma —current developments and understanding
AbstractPenile squamous cell carcinoma is a rare malignancy with various distinct histological subtypes, each with distinct appearances, histotypic specific associations with human papillomavirus (HPV) and clinical behaviour. Despite a wealth of pathological knowledge, there still remains a limited understanding of the fundamental molecular drivers that govern penile carcinogenesis with their underlying prognostic and therapeutic importance. However, recent work has improved our fundamental understanding of the molecular pathogenesis of penile cancer: commonly divided into the HPV-dependent and HPV-independent pathways. Th...
Source: Virchows Archiv - June 26, 2019 Category: Pathology Source Type: research

Predicting outcomes in non-muscle invasive (Ta/T1) bladder cancer: the role of molecular grade based on luminal/basal phenotype
AbstractBladder cancer tumors can be divided into two molecular subtypes referred to as luminal or basal. Each subtype may react differently to current chemotherapy or immunotherapy. Likewise, the technology required for comprehensive molecular analysis is expensive and not yet applicable for routine clinical diagnostics. Therefore, it has been suggested that the immunohistochemical expressions of only two markers, luminal (CK20+, CK5/6 –) and basal (CK5/6+, CK20–), is sufficient to identify the molecular subtypes of bladder cancer. This would represent a molecular grade that could be used in daily practice. Mo...
Source: Virchows Archiv - June 25, 2019 Category: Pathology Source Type: research

IDH1 immunohistochemistry reactivity and mosaic IDH1 or IDH2 somatic mutations in pediatric sporadic enchondroma and enchondromatosis
AbstractMosaic somatic mutations in the isocitrate dehydrogenase 1/2 (IDH1/2) genes have been identified in most enchondromas by targeted mutation analysis. Next-generation sequencing (NGS), that may detect even low-level mosaic mutation rates, has not previously been applied to enchondromas. Immunohistochemistry using the H09 clone is routinely used as a surrogate for the common R132HIDH1 mutation in gliomas. We compared immunohistochemistry and NGS results in a series of 13 enchondromas from 8 pediatric patients. NGS identified a heterozygousIDH mutation in all enchondromas, showing identical mutation status in patients ...
Source: Virchows Archiv - June 25, 2019 Category: Pathology Source Type: research

Machine learning approaches for pathologic diagnosis
AbstractMachine learning techniques, especially deep learning techniques such as convolutional neural networks, have been successfully applied to general image recognitions since their overwhelming performance at the 2012 ImageNet Large Scale Visual Recognition Challenge. Recently, such techniques have also been applied to various medical, including histopathological, images to assist the process of medical diagnosis. In some cases, deep learning –based algorithms have already outperformed experienced pathologists for recognition of histopathological images. However, pathological images differ from general images in ...
Source: Virchows Archiv - June 20, 2019 Category: Pathology Source Type: research

Two cases of phenotypic switch of primary cutaneous T cell lymphoma after treatment with an aggressive course and review of the literature
We report two cases of pa tients that were diagnosed with a CD4+ mycosis fungoides with positive T cell receptor gene rearrangement studies. Both patients originally responded to treatment, but subsequently, their CTCL came back with a different phenotype of a CD4− CTCL. Gene rearrangement studies were performed on the sec ond occurrence in order to prove that this was the same lymphoma. Both patients died from their CTCL. Additionally, we collected seven cases of primary CTCL from the literature with tissue samples from before and after treatment with molecular studies confirming these neoplasms contained the same T...
Source: Virchows Archiv - June 19, 2019 Category: Pathology Source Type: research

CD39 downregulation in chronic intervillositis of unknown etiology
AbstractChronic intervillositis of unknown etiology (CIUE) is a rare placental lesion associated with infiltration of mononuclear inflammatory cells into the intervillous space, poor perinatal outcomes (intrauterine fetal demise or fetal growth restriction), and high rates of recurrence. CD39 is the ectonucleotidase that protects tissues from inflammatory stress and cell injury, which is localized on the surface of villi in normal placentas; however, its expression and role in CIUE are unknown. The aims of this retrospective study were to determine the expression of CD39 in CIUE and its significance in pregnancy outcomes. ...
Source: Virchows Archiv - June 19, 2019 Category: Pathology Source Type: research

Pathologist second opinion significantly alters clinical management of pT1 endoscopically resected colorectal cancer
In conclusion, second opinion significantly alters risk perception of endoscopically removed early colorectal carcinomas representing a valuable tool for their appropriate clinical management. (Source: Virchows Archiv)
Source: Virchows Archiv - June 17, 2019 Category: Pathology Source Type: research

Recent advances in invasive adenocarcinoma of the cervix
AbstractEndocervical adenocarcinomas (ECAs) are currently classified according to the 2014 World Health Organization (WHO) system, which is predominantly based on descriptive morphologic characteristics, considers factors bearing minimal etiological, clinical, or therapeutic relevance, and lacks sufficient reproducibility. The 2017 International Endocervical Adenocarcinoma Criteria and Classification (IECC) system was developed by a group of international collaborators to address these limitations. The IECC system separates ECAs into two major groups —those that are human papillomavirus-associated (HPVA) and those th...
Source: Virchows Archiv - June 17, 2019 Category: Pathology Source Type: research

Immunohistochemical expression of mismatch repair proteins (MSH2, MSH6, MLH1, and PMS2) in prostate cancer: correlation with grade groups (WHO 2016) and ERG and PTEN status
AbstractThe role of DNA MMR genes in prostate cancer (PrCa) is controversial, as genetic alterations leading to microsatellite instability are incompletely defined in these tumors.ERG rearrangements andPTEN loss are concomitant events in PrCa. The aim of this study has been to analyze the immunohistochemical (IHC) expression of MSH2, MSH6, MLH1, PMS2, ERG, and PTEN and their potential association with the grade group (GG) grading system (WHO 2016) and PSA recurrence in a series of 200 PrCa (PSMAR-Biobank, Barcelona, Spain). MSH2, MLH1, PMS2, and PTEN losses were documented in 8%, 5%, 2%, and 36.5%, respectively. ERG expres...
Source: Virchows Archiv - June 17, 2019 Category: Pathology Source Type: research

Identification of a novel PRR15L-RSPO2 fusion transcript in a sigmoid colon cancer derived from superficially serrated adenoma
AbstractSuperficially serrated adenoma (SuSA) is a recently proposed subtype of colorectal serrated lesion. We here report a sigmoid colon cancer derived from SuSA, which exhibited aggressive clinical behavior. Endoscopically, the tumor appeared as a superficial elevated lesion with a large nodule. Histological examination of the surgically resected material showed tubular adenocarcinoma associated with SuSA. Although tumor invasion was limited to the submucosal layer, lymph node and extranodal metastases were detected. The patient subsequently developed peritoneal metastases and died 15  months after surgery. Molecul...
Source: Virchows Archiv - June 17, 2019 Category: Pathology Source Type: research

Follicular dendritic cells display microvesicle-associated LMP1 in reactive germinal centers of EBV+ classic Hodgkin lymphoma
AbstractExpression of the latent membrane protein-1 (LMP1) of Epstein-Barr virus (EBV) was investigated in 153 cases of EBV+ classic Hodgkin lymphoma (cHL); 120 cases were pediatric patients (
Source: Virchows Archiv - June 15, 2019 Category: Pathology Source Type: research

Spread through air spaces (STAS) is a predictor of poor outcome in atypical carcinoids of the lung
In conclusion, the presence of STAS is an additional relevant adverse prognostic factor in pulmonary AC that currently has the most unpredictable outcome and the most controversial treatment strategy. (Source: Virchows Archiv)
Source: Virchows Archiv - June 15, 2019 Category: Pathology Source Type: research

Constitutional abnormality of nuclear membrane proteins in small cell lung carcinoma
In conclusion, we suggest tha t downregulation of the nuclear membrane proteins emerin and lamin A/C is characteristic of SCLC cells, and this constitutional abnormality of the nuclear membrane may be related to the biological and/or clinical nature of SCLC. In addition, knowing the nuclear protein profile in SCLC cells may cont ribute to our understanding of nuclear fragility known as the crush artifact in pulmonary biopsy specimens. (Source: Virchows Archiv)
Source: Virchows Archiv - June 15, 2019 Category: Pathology Source Type: research

Performance of the minimally invasive autopsy tool for cause of death determination in adult deaths from the Brazilian Amazon: an observational study
In conclusion, the MIA showed a substantial performance for CoD identification. Clinico-pathological discrepancies remain high and justify the need for post-mortem studies, even in referral hospitals. The MIA is a robust substitute of th e CDA for CoD surveillance and quality improvement of clinical practice in low- and middle-income settings. (Source: Virchows Archiv)
Source: Virchows Archiv - June 14, 2019 Category: Pathology Source Type: research

Sinusoidal and pericellular fibrosis in adult post-transplant liver biopsies: association with hepatic stellate cell activation and patient outcome
AbstractPost-transplant sinusoidal fibrosis (SF) and pericellular fibrosis (PCF) have not been extensively investigated in adults. Fifty-two post-transplant liver biopsies from 28 consented patients (12 men, mean age 49, range 33 –67 years) were studied. Tissue morphology, including an arbitrary summative fibrosis score was assessed in detail. Collagen proportionate area (CPA) and alpha-smooth muscle actin (α-SMA) immunostain were evaluated by digital image analysis (DIA). Anti-keratin 7, anti-C4d and anti-sonic hedgehog (Shh) immunostains were scored semi-quantitatively. SF was observed in 36/52 (69.2%) b...
Source: Virchows Archiv - June 14, 2019 Category: Pathology Source Type: research

Mist1: a novel nuclear marker for acinic cell carcinoma of the salivary gland
AbstractMuscle, intestine, and stomach expression 1 (Mist1) is a transcription factor that functions in the development of granule organisation in serous exocrine cells of the gastrointestinal tract. The aim of this study was to investigate whether Mist1 can be used as a marker for acinic cell carcinoma (AciCC) of the salivary gland. Immunohistochemistry (IHC) was used to analyse Mist1 expression in AciCC (n = 26), secretory carcinoma (SC;n = 18) and 12 other types of salivary gland tumours (n = 99). Strong Mist1 staining was observed in normal serous acinar cells and plasma cells....
Source: Virchows Archiv - June 11, 2019 Category: Pathology Source Type: research

Sarcomatoid carcinomas of the gallbladder: clinicopathologic characteristics
In this study, 656 gallbladder carcinomas (GBC) were reviewed. Eleven (1.7%) with a sarcomatoid component were identified and analyzed in comparison with ordinary GBC (O-GBC). Patients included 9 females a nd 2 males (F/M = 4.5 vs. 3.9) with a mean age-at-diagnosis of 71 (vs. 64). The median tumor size was 4.6 cm (vs. 2.5;P = 0.01). Nine patients (84%) presented with advanced stage (pT3/4) tumor (vs. 48%). An adenocarcinoma component constituting 1–75% of the tumor was present in nine, and eight had surface dysplasia/CIS; either in situ or invasive carcinoma was present in all cases. ...
Source: Virchows Archiv - June 8, 2019 Category: Pathology Source Type: research

In this issue
(Source: Virchows Archiv)
Source: Virchows Archiv - June 8, 2019 Category: Pathology Source Type: research

Synoptic reporting increases quality of upper gastrointestinal cancer pathology reports
AbstractIntroductionTraditionally, surgical pathology reports are narrative. These report types are prone to error and missing data; therefore, structured standardized reporting was introduced. However, the effect of synoptic reporting on the completeness of esophageal and gastric carcinoma pathology reports is not yet established.Materials and methodsA population-based retrospective nationwide cohort study in the Netherlands was conducted over a period of 2012 –2016, utilizing the Netherlands Cancer Registry for patient data and the nationwide network and registry of histology for pathology data.ResultsIn total, 114...
Source: Virchows Archiv - May 29, 2019 Category: Pathology Source Type: research

Prognostic significance of combining immunohistochemical markers for cancer-associated fibroblasts in lung adenocarcinoma tissue
AbstractCancer-associated fibroblasts (CAFs), activated fibroblasts in a cancer microenvironment, exert various effects upon carcinoma cells including lung adenocarcinoma cells. Various markers identifying CAFs have been proposed, but the correlations among these markers proposed and their clinicopathological significance have remained largely unknown. Therefore, in this study, we immunohistochemically evaluated the expression of alpha-smooth muscle actin ( α-SMA), podoplanin, and periostin among these proposed markers in 92 cases of lung adenocarcinoma. These three markers were weakly correlated, but the relative ab...
Source: Virchows Archiv - May 27, 2019 Category: Pathology Source Type: research

PD-L1 testing of non-small cell lung cancer using different antibodies and platforms: a Swiss cross-validation study
AbstractWith the approval of pembrolizumab for first- and second-line treatment of PD-L1+ non-small cell lung cancer (NSCLC), PD-L1 testing by immunohistochemistry (IHC) has become a necessity. However, the DAKO autostainer ASL48 for the FDA approved DAKO 22C3 pharmDx assay is not broadly available in Switzerland and other parts of Europe. The primary goal of this study was to cross-validate the 22C3 anti-PD-L1 antibody on Benchmark Ultra (VBMU) and Leica Bond (LBO) immunostainers. IHC protocols were developed for 22C3 on both platforms with the 22C3phDx using ASL48 as reference. A tissue microarray (TMA) was constructed f...
Source: Virchows Archiv - May 24, 2019 Category: Pathology Source Type: research

Transient receptor potential vanilloid (TRPV) channel expression in meningiomas: prognostic and predictive significance
AbstractThe TRPV1 –4 members of TRPV cation channel subfamily are mainly regarded as polymodal receptors that may be activated by diverse changes in cellular microenvironment and endogenous and exogenous agents. Abnormal expression of these channels has been reported in various tumors but not in meningiomas. Mening ioma cells are thought to originate from arachnoid cap cells due to cytological and functional similarities between the two types of cells. To investigate the expression profile of TRPV1–4 channels in meningiomas and compare with TRPV1–4 channel expression in leptomeninges, we used immunohistoc...
Source: Virchows Archiv - May 22, 2019 Category: Pathology Source Type: research

Low-grade endometrial stromal sarcoma with a novel MEAF6-SUZ12 fusion
AbstractEndometrial stromal sarcoma (ESS) is a rare mesenchymal neoplasm. Herein, we report a low-grade ESS with a novelMEAF6-SUZ12 fusion gene. A 40-year-old woman presented with a 9.0-cm abdominal wall mass juxtaposed to the postoperative scar of surgeries for uterine “leiomyomas” and cesarean section. Histologically, mostly hypocellular and myxoid nodules were comprised of uniform spindle cells and exhibited tongue-like infiltration. Immunohistochemically, the tumor cells were positive for CD10, estrogen receptor, and CD34 (focal). There were occasional h-ca ldesmon-positive cohesive nests. RNA sequencing al...
Source: Virchows Archiv - May 17, 2019 Category: Pathology Source Type: research

Deep learning for automatic Gleason pattern classification for grade group determination of prostate biopsies
AbstractHistopathologic grading of prostate cancer using Gleason patterns (GPs) is subject to a large inter-observer variability, which may result in suboptimal treatment of patients. With the introduction of digitization and whole-slide images of prostate biopsies, computer-aided grading becomes feasible. Computer-aided grading has the potential to improve histopathological grading and treatment selection for prostate cancer. Automated detection of GPs and determination of the grade groups (GG) using a convolutional neural network. In total, 96 prostate biopsies from 38 patients are annotated on pixel-level. Automated det...
Source: Virchows Archiv - May 16, 2019 Category: Pathology Source Type: research

Dataset for the reporting of prostate carcinoma in radical prostatectomy specimens: updated recommendations from the International Collaboration on Cancer Reporting
This article presents the up-to-date, evidence-based ICCR dataset and associated commentary for reporting prostate cancer in radical prostatectomy specimens. PubMed and Google search engines were used to review the published literatu re on the subject, and the dataset was developed in line with the previously published ICCR framework for the development of cancer datasets. Substantial changes have been incorporated into the second edition of the ICCR prostate cancer (radical prostatectomy) dataset. These include revisions to pro state cancer grading, reporting of intraductal carcinoma of prostate and surgical margins, amon...
Source: Virchows Archiv - May 16, 2019 Category: Pathology Source Type: research

Emerging relationships between papillary proliferation of the endometrium and endometrial carcinoma: evidence from an immunohistochemical and molecular analysis
In this study, we analyzed the clinicopathological and immunohistochemical features in 30 PPEs (22 simple PPEs and 8 complex papillary hyperplasia (CPH)). Hotspot mutations ofKRAS,PI3KCA,AKT1,PTEN (exons 3, 5, and 7), andARID1A (exons 1 and 14) were detected by pyrosequencing or bidirectional Sanger sequencing. We found that endometrial hyperplasia and carcinoma were more common in CPHs (4/6, 66.7%) than in simple PPEs (4/21, 19.0%) (p   0.05).KRAS mutations were identified in 14 PPEs and 1 concurrent endometrial carcinoma. The prevalence of KRAS mutations was not statistically different between simple PPEs (...
Source: Virchows Archiv - May 12, 2019 Category: Pathology Source Type: research

Prognostic significance of histological categorization of desmoplastic reaction in colorectal liver metastases
In conclusion, DRliver categorization may be valuable for predicting prognosis after hepatectomy among patients with CRLM. (Source: Virchows Archiv)
Source: Virchows Archiv - May 10, 2019 Category: Pathology Source Type: research

Book Review —Diagnostic pathology: placenta, second edition
(Source: Virchows Archiv)
Source: Virchows Archiv - May 10, 2019 Category: Pathology Source Type: research

IL-33 in the tumor microenvironment is associated with the accumulation of FoxP3-positive regulatory T cells in human esophageal carcinomas
In this study, we have therefore characterized the expression of IL-33 by immunohistochemistry (IHC) and double immunofluorescence staining and analyzed its relationship with FoxP3+ Treg accumulation in the microenvironment in 80 patients with ESCC. IHC observation revealed a high expression level of IL-33 in both ESCC mass and stroma, which paralleled to a high density of FoxP3+ Tregs accumulated in the same compartments. Statistical analysis showed that the scores for cell densities of tumor- and stroma-expressing IL-33 were significantly correlated with the scores for density of FoxP3+ Tregs in the tumor stroma. Further...
Source: Virchows Archiv - May 6, 2019 Category: Pathology Source Type: research

Mutation in BRAF and SMAD4 associated with resistance to neoadjuvant chemoradiation therapy in locally advanced rectal cancer
AbstractOur study was done in order to identify novel molecular markers to predict which locally advanced rectal cancers (LARCs) might be resistant to neoadjuvant chemoradiotherapy (nCRT). Seventy-four patients with LARCs treated with nCRT were collected. Pathological evaluation after nCRT was performed according to the tumor regression grading (TRG) system. Next-generation sequencing kit including 279 exons of 59 genes was performed on Illumina Miseq Platform. Sanger sequencing was performed to confirm some mutations. Four of the tumors (4/74, 5.4%) hadBRAF mutation, which presented in one TRG 2 tumor and three TRG 3 tumo...
Source: Virchows Archiv - May 6, 2019 Category: Pathology Source Type: research

Incidental lymphoma in lymph node dissection for carcinoma in the abdominopelvic cavity: a single-institution experience
AbstractIncidental detection of lymphoma in lymph node (LN) dissection for carcinoma is extremely rare. The occurrence and clinicopathological features of this rare condition have not been characterised. The medical records of 11,889 consecutive patients who underwent LN dissection for carcinoma in the abdominopelvic cavity were retrospectively reviewed. Among these patients, 11 had lymphomas detected in LN dissections and 7 had no previous history of lymphoma, representing an incidental detection rate of 0.06% (7/11889). The patients had a median age of 63  years (range, 48–69 years), and the male-to-femal...
Source: Virchows Archiv - May 2, 2019 Category: Pathology Source Type: research

IQN path ASBL report from the first European cfDNA consensus meeting: expert opinion on the minimal requirements for clinical ctDNA testing
AbstractLiquid biopsy testing is a new laboratory-based method that detects tumour mutations in circulating free DNA (cfDNA) derived from minimally invasive blood sampling techniques. Recognising the significance for clinical testing, in 2017, IQN Path provided external quality assessment for liquid biopsy testing. Representatives of those participating laboratories were invited to attend a workshop to discuss the findings and how to achieve quality implementation of cfDNA testing in the clinical setting, the discussion and outcomes of this consensus meeting are described below. Predictive molecular profiling using tumour ...
Source: Virchows Archiv - April 26, 2019 Category: Pathology Source Type: research

In this issue
(Source: Virchows Archiv)
Source: Virchows Archiv - April 24, 2019 Category: Pathology Source Type: research

In this issue May
(Source: Virchows Archiv)
Source: Virchows Archiv - April 24, 2019 Category: Pathology Source Type: research