Breast tissue imaging atlas using ultra-fast confocal microscopy to identify cancer lesions
The objective of the study was to create an atlas of ex vivo UFCM images of breast tissues and breast carcinomas based on the first extensive collection of large field-of-view UFCM breast images. One hundred sixty patients who underwent conserving surgery for breast cancer were included. Their fresh surgical specimens were sliced, stained with acridine orange, and imaged at high resolution with large-field-of-view UFCM. The resulting images were digitally false colored to resemble frozen sections. Each UFCM image was correlated with the corresponding definitive histology. Representative images of normal tissue, inflammatio...
Source: Virchows Archiv - March 19, 2024 Category: Pathology Source Type: research
Prognostic implications of tumor histology and microenvironment in surgically resected intrahepatic cholangiocarcinoma: a single institutional experience
AbstractIntrahepatic cholangiocarcinoma (ICC) is a highly aggressive malignant neoplasm. Certain histologic features and the tumor microenvironment may impact disease progression. We aim to characterize the clinicopathologic features of ICC to identify prognostic factors. A total of 50 surgically resected ICC (partial or transplant) cases were analyzed. The cohort included 26 men and 24 women with a median age of 62 years. Eighteen (36%) cases were multifocal ICC with a mean largest tumor size of 6.5 cm. Neoadjuvant and adjuvant chemotherapy was done in eight (16%) and 33 (66%) patients, respectively. Histologically, 42...
Source: Virchows Archiv - March 19, 2024 Category: Pathology Source Type: research
P53 Overexpression May Represent an Early Marker of Clinicopathologic Progression in Vasculogenic Mesenchymal Lesions of Germ Cell Tumor Origin
AbstractVasculogenic mesenchymal lesions (VMLs) of germ cell tumor origin are thought to originate in postpubertal-type yolk sac tumor components and include a spectrum of lesions from teratoma with vasculogenic stroma (TVS), to low and high-grade vasculogenic mesenchymal tumors (VMTs). VMLs exhibit rudimentary to well-developed neoplastic vessels within primitive mesenchyme, being considered a neoplastic reiteration of embryonic vasculogen esis in the splanchnic mesoderm of the yolk sac. They occur in patients with primary mediastinal germ cell tumors after chemotherapy, and a subset progresses to “somatic-type” sar...
Source: Virchows Archiv - March 19, 2024 Category: Pathology Source Type: research
Perinatal arterial ischemic stroke: how informative is the placenta?
We report here the histological placental findings in a series of 16 neonates with radiologically confirmed PAIS. Findings were grouped into 3 categories of lesions: (1) inflammation, (2) placental and fetal hypoxic lesions, and (3) placentas with a high birthweight/placenta weight ratio. Matched control placentas were compared to the pathological placentas when feasible. The eight term singleton placentas were compared to a series of 20 placentas from a highly controlled amniotic membrane donation program; in three twin pregnancies, the placental portions from the affected twin and unaffected co-twin were compared. Slight...
Source: Virchows Archiv - March 19, 2024 Category: Pathology Source Type: research
Molecularly defined sinonasal malignancies: an overview with focus on the current WHO classification and recently described provisional entities
AbstractClassification of tumors of the head and neck has evolved in recent decades including a widespread application of molecular testing in tumors of the sinonasal tract, salivary glands, and soft tissues with a predilection for the head and neck. The availability of new molecular techniques has allowed for the definition of multiple novel tumor types unique to head and neck sites. Moreover, an expanding spectrum of immunohistochemical markers specific to genetic alterations facilitates rapid identification of diagnostic molecular abnormalities. As such, it is currently possible for head and neck pathologists to benefit...
Source: Virchows Archiv - March 16, 2024 Category: Pathology Source Type: research
Multicenter evaluation of an automated, multiplex, RNA-based molecular assay for detection of ALK, ROS1, RET fusions and MET exon 14 skipping in NSCLC
AbstractThe current study assessed the performance of the fully automated RT-PCR-based Idylla ™ GeneFusion Assay, which simultaneously covers the advanced non-small cell lung carcinoma (aNSCLC) actionableALK,ROS1,RET, andMET exon 14 rearrangements, in a routine clinical setting involving 12 European clinical centers. The Idylla ™ GeneFusion Assay detects fusions using fusion-specific as well as expression imbalance detection, the latter enabling detection of uncommon fusions not covered by fusion-specific assays. In total, 326 archival aNSCLC formalin-fixed paraffin-embedded (FFPE) samples were included of which 44% we...
Source: Virchows Archiv - March 16, 2024 Category: Pathology Source Type: research
Clinical significance of location of perineural cancer invasion detected on prostate needle core biopsy
AbstractThe clinical impact of site-specific perineural invasion (PNI) in prostate cancer remains poorly understood. We compared radical prostatectomy findings and oncologic outcomes in 434 patients with single-site PNI on systematic sextant biopsy. PNI was present in the right apex (n = 62; 14%), right mid (n = 70; 16%), right base (n = 89; 21%), left apex (n = 64; 15%), left mid (n = 58; 13%), and left base (n = 91; 21%). There were no significant differences in biopsy or prostatectomy findings, when comparing apex vs. mid vs. base PNI. Univariate analysis revealed that apex-localized PNI was asso...
Source: Virchows Archiv - March 15, 2024 Category: Pathology Source Type: research
The diagnostic utility of cytology specimen in a case of EWSR1::NFATC2 sarcoma
This report describes the cytological features ofEWSR1::NFATC2 sarcoma and highlights the diagnostic utility of archival cytology specimens. (Source: Virchows Archiv)
Source: Virchows Archiv - March 14, 2024 Category: Pathology Source Type: research
Epidermodysplasia verruciformis –associated eccrine neoplasm: a rare entity with distinctive clinical and histopathologic features
We report here a distinctive form of EDV-associated eccrine neoplasia. Seven tumors from two patients were analyzed and show highly uniform features including multiple clustered clinical lesions, multifocal epidermal origin, eccrine differentiation with close association with the acrosyringium, an anastomosing growth pattern, and a bland monotonous poroid-to-basaloid cytomorphology. Clinical follow-up for one patient has been benign to date. These tumors show strong similarity to two previously reported cases, suggesting that this type of EDV-associated eccrine neoplasia may represent a rare but reproducible form of skin a...
Source: Virchows Archiv - March 14, 2024 Category: Pathology Source Type: research
Predominance of T-bet-positive Th1 cells in infiltrating T-lymphocytes in most of active arteritis lesions of giant cell arteritis
AbstractImmunological mechanisms through the activation of CD4-positive T-cells have been assumed to be involved in the pathogenesis of giant cell arteritis (GCA). Many studies employing frozen tissues of temporal artery biopsy, peripheral blood lymphocytes, and plasma of GCA patients have revealed the contribution of interferon- γ and interleukin-17 in both protein and mRNA levels. However, the analyses using formalin-fixed and paraffin-embedded (FFPE) tissue specimens, in which the correlation between histopathologic pictures and immunological circumstances would be elucidated, have been limited. Here, we performed the ...
Source: Virchows Archiv - March 13, 2024 Category: Pathology Source Type: research
Clinicopathological analysis of thyroid carcinomas with the RET and NTRK fusion genes: characterization for genetic analysis
This study characterized the pathological findings associated with these genes to identify the specimens suitable for genetic analysis. Thyroid carcinoma cases positive for the fusion genes were analyzed using the Oncomine Dx Target Test. Clinicopathological data were collected and assessed. Among the 74 patients tested, 8 hadRET and 1 hadNTRK3 fusion gene. Specifically, of theRET fusion gene cases, 6 exhibited “BRAF-like ” atypia and 2 showed “RAS-like ” atypia, while the single case with anNTRK3 fusion gene presented “RAS-like ” atypia. Apart from one poorly differentiated thyroid carcinoma, most cases involv...
Source: Virchows Archiv - March 12, 2024 Category: Pathology Source Type: research
Flanagan ’s condensed protocol for neurodegenerative diseases. Implementation in a clinical autopsy setting with partial supervision of a neuropathologist
AbstractThe Condensed Protocol (CP) was originally developed for the evaluation of Alzheimer ’s Disease (AD) and other neurodegenerative diseases as a workable alternative to the complex and costly established autopsy guidelines. The study objective is to examine the degree of implementation of the CP in the pathology department of a third level university hospital in a period of 5 years . Clinical autopsies performed between 2016 and 2021 on patients aged 65 years or over and did not require a specific neuropathological examination were reviewed. Histological screening and staging of neurodegenerative diseases was per...
Source: Virchows Archiv - March 12, 2024 Category: Pathology Source Type: research
Mutational, immune microenvironment, and clinicopathological profiles of diffuse large B-cell lymphoma and follicular lymphoma with BCL6 rearrangement
In conclusion, bothBCL6-R-positive FL andBCL6-R-positive DLBCL had a common mutational profile; but also, differences. DLBCL cases had a higher density of microenvironment markers. (Source: Virchows Archiv)
Source: Virchows Archiv - March 11, 2024 Category: Pathology Source Type: research
The enigma of glutamine synthetase and b-catenin expression in hepatocellular adenoma in familial adenomatous polyposis coli
(Source: Virchows Archiv)
Source: Virchows Archiv - March 8, 2024 Category: Pathology Source Type: research
Molecular diagnostics of hepatobiliary and pancreatic neoplasias
AbstractNeoplasias of the hepatopancreatobiliary tract are growing in numbers, have the poorest prognosis of all major cancer entities, and thus represent a rising clinical problem. Their molecular diagnostic has dramatically improved, contributing to tumor subtyping, definition of malignancy, and uncovering cases with hereditary predisposition. Most of all, predictive molecular testing allows to identify cases amenable to treatment with the rising number of approved targeted drugs, immune-oncological treatment, and clinical trials. In this review, the current state of molecular testing and its contribution to clinical dec...
Source: Virchows Archiv - March 1, 2024 Category: Pathology Source Type: research
