Peroxisome Proliferator-Activated Receptor < em > γ < /em > Regulates Lipid Metabolism in Sheep Trophoblast Cells through mTOR Pathway-Mediated Autophagy
In this study, we used PPARγ agonist rosiglitazone and inhibitor GW9662, as well as autophagy activator rapamycin and inhibitor 3-methyladenine, to unravel the probable mechanism of PPARγ engaged in lipid metabolism in sheep trophoblast cells (STCs). After 12 h, 24 h, and 48 h of drug treatment, the levels of autophagy-related proteins were detected by Western blot, the triglyceride content and MDA level of cells were detected by colorimetry, and the lipid droplets and lysosomes were localized by immunofluorescence. We found that PPARγ inhibited the activity of mammalian target of rapamycin (mTOR) pathway in STCs for a ...
Source: PPAR Research - November 29, 2023 Category: Genetics & Stem Cells Authors: Kexing Hao Jing Wang Hengbin Yu Lei Chen Weibin Zeng Zhengrong Wang Guangdong Hu Source Type: research
