A new heparan sulfate from the mollusk Nodipecten nodosus inhibits merozoite invasion and disrupts rosetting and cytoadherence of Plasmodium falciparum.
CONCLUSIONS: These findings support a potential use for mollusk HS as adjunct therapy for severe malaria. PMID: 31188952 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - June 14, 2019 Category: Infectious Diseases Authors: Bastos MF, Albrecht L, Gomes AM, Lopes SC, Vicente CP, de Almeida RP, Cassiano GC, Fonseca RJ, Werneck CC, Pavão MS, Costa FT Tags: Mem Inst Oswaldo Cruz Source Type: research

Criteria for identification of Schistosoma mansoni eggs in faecal sediments prepared with the Helmintex method and stained by ninhydrin.
In conclusion, ninhydrin staining and a combination of criteria are recommended for microscope examination of faecal sediments. PMID: 31166420 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - June 7, 2019 Category: Infectious Diseases Authors: de Souza RP, Favero V, Pascoal VF, Lindholz C, Bittencourt HR, Graeff-Teixeira C Tags: Mem Inst Oswaldo Cruz Source Type: research

Diversity of metallo- β-lactamase-encoding genes found in distinct species of Acinetobacter isolated from the Brazilian Amazon Region.
CONCLUSIONS: To the best of our knowledge, this is the first report describing the dissemination of bla NDM-1 among distinct Acinetobacter species recovered from the same hospital in South America. PMID: 31166421 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - June 7, 2019 Category: Infectious Diseases Authors: Brasiliense D, Cayô R, Streling AP, Nodari CS, Barata RR, Lemos PS, Massafra JM, Correa Y, Magalhães I, Gales AC, Sodré R Tags: Mem Inst Oswaldo Cruz Source Type: research

Rapid detection of human blood in triatomines (kissing bugs) utilizing a lateral flow immunochromatographic assay - A pilot study.
CONCLUSIONS: The pilot study shows that this rapid and specific test may have applications in triatomine research. Further study is needed to determine the sensitivity of this assay compared to other well-established techniques, such as DNA- and proteomics-based methodologies and the assay's application in the field. PMID: 31166422 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - June 7, 2019 Category: Infectious Diseases Authors: Beatty NL, Behrens-Bradley N, Love M, McCants F, Smith S, Schmidt JO, Hamer SA, Dorn PL, Ahmad N, Klotz SA Tags: Mem Inst Oswaldo Cruz Source Type: research

Effects of atmospheric oscillations on infectious diseases: the case of Chagas disease in Chile.
CONCLUSIONS: The Chagas disease frequency per year in Chile was found to depend mainly on SOI in previous year, whose values can be determined one year in advance. Therefore, it is possible to partially forecast annual frequency patterns. This could have important applications in public health strategies and for allocating resources for the management of the disease. PMID: 31166479 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - June 7, 2019 Category: Infectious Diseases Authors: Báez JC, Olivero J, Salazar-Aravena LE, Suazo-Galdames IC Tags: Mem Inst Oswaldo Cruz Source Type: research

Evolutionary study of potentially zoonotic hepatitis E virus genotype 3 from swine in Northeast Brazil.
il LH Abstract Hepatitis E virus (HEV), an emerging virus associated with acute hepatic disease, leads to thousands of deaths worldwide. HEV has already been reported in Brazil; however, there is a lack of epidemiological and molecular information on the genetic variability, taxonomy, and evolution of HEV. It is thus unclear whether hepatitis E is a neglected disease in Brazil or it has low relevance for public health in this country. Here, for the first time, we report the presence of HEV in Northeast Brazil. A total of 119 swine faecal samples were screened for the presence of HEV RNA using real-time polymerase ...
Source: Memorias do Instituto Oswaldo Cruz - June 7, 2019 Category: Infectious Diseases Authors: Oliveira-Filho EF, Dos Santos DR, Durães-Carvalho R, da Silva A, de Lima GB, Batista Filho AFB, Pena LJ, Gil LH Tags: Mem Inst Oswaldo Cruz Source Type: research

Genome-wide identification, characterisation and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata.
CONCLUSIONS: Our results show the presence, conservation and expression profile of the UPS in this mollusk, providing a basis and new knowledge for other studies involving this system. Due to the importance of the UPS and B. glabrata, this work may influence the search for new methodologies for the control of schistosomiasis. PMID: 31166481 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - June 7, 2019 Category: Infectious Diseases Authors: Portilho LG, Duarte BCD, Queiroz FR, Ribeiro THC, Jeremias WJ, Babá EH, Coelho PMZ, Morais ER, Cabral FJ, Caldeira RL, Gomes MS Tags: Mem Inst Oswaldo Cruz Source Type: research

Apor é virus, a novel mammarenavirus (Bunyavirales: Arenaviridae) related to highly pathogenic virus from South America.
Aporé virus, a novel mammarenavirus (Bunyavirales: Arenaviridae) related to highly pathogenic virus from South America. Mem Inst Oswaldo Cruz. 2019;114:e180586 Authors: Fernandes J, Guterres A, de Oliveira RC, Jardim R, Dávila AMR, Hewson R, de Lemos ERS Abstract Here, we report the complete genome sequence of the Aporé virus (Bunyavirales: Arenaviridae), obtained from a wild rodent Oligoryzomys mattogrossae captured in Mato Grosso do Sul state, Brazil. The genome of this virus showed strong similarity to highly pathogenic mammarenavirus from South America. PMID: 31141019 [Pu...
Source: Memorias do Instituto Oswaldo Cruz - May 31, 2019 Category: Infectious Diseases Authors: Fernandes J, Guterres A, de Oliveira RC, Jardim R, Dávila AMR, Hewson R, de Lemos ERS Tags: Mem Inst Oswaldo Cruz Source Type: research

Association between MBL2 haplotypes and dengue severity in children from Rio de Janeiro, Brazil.
CONCLUSIONS: This is the first study to demonstrate the association between MBL2 haplotypes and dengue severity in Brazilians including adjustment for genetic ancestry. These results reinforce the role of mannose binding lectin in immune response to DENV. PMID: 31141020 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - May 31, 2019 Category: Infectious Diseases Authors: Ornelas AMM, Xavier-de-Carvalho C, Alvarado-Arnez LE, Ribeiro-Alves M, Rossi ÁD, Tanuri A, de Aguiar RS, Moraes MO, Cardoso CC Tags: Mem Inst Oswaldo Cruz Source Type: research

Analysis of COI and ITS2 regions of DNA obtained from Paragonimus westermani eggs in ancient coprolites on Joseon dynasty mummies.
In this study, we found that ancient P. westermani sequences in Korea belong to Group I, adding invaluable information to the existing knowledge of Paragonimus paleogenetics. PMID: 31116227 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - May 24, 2019 Category: Infectious Diseases Authors: Hong JH, Oh CS, Seo M, Shin DH Tags: Mem Inst Oswaldo Cruz Source Type: research

Development of Leishmania mexicana in Lutzomyia longipalpis in the absence of sugar feeding.
Abstract The leishmaniases are caused by Leishmania parasites and transmitted through the bites of phlebotomine sand flies. During parasite development inside the vector's midgut, promastigotes move towards the stomodeal valve, a mechanism that is crucial for transmission. It has been reported that the sugar meal acquired by sand flies during feeding between bloodmeals is essential for the development and migration of parasites. We demonstrated that the distribution of Leishmania mexicana parasites was affected by the sugar meals obtained by the sand flies. Promastigote migration towards the cardia region seems to...
Source: Memorias do Instituto Oswaldo Cruz - May 24, 2019 Category: Infectious Diseases Authors: da Costa SG, Moraes CDS, Bates P, Dillon R, Genta FA Tags: Mem Inst Oswaldo Cruz Source Type: research

Non-clonal occurrence of pmrB mutations associated with polymyxin resistance in carbapenem-resistant Klebsiella pneumoniae in Brazil.
CONCLUSIONS: Deleterious mutations in pmrB gene is the main chromosomal target for induction of polymyxin resistance in carbapenem-resistant K. pneumoniae in public hospitals in the Mid-West of Brazil. PMID: 31116243 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - May 24, 2019 Category: Infectious Diseases Authors: Rodrigues ACS, Santos ICO, Campos CC, Rezende IN, Ferreira YM, Chaves CEV, Rocha-de-Souza CM, Carvalho-Assef APD, Chang MR Tags: Mem Inst Oswaldo Cruz Source Type: research

Evaluation of pathogen P21 protein as a potential modulator of the protective immunity induced by Trypanosoma cruzi attenuated parasites.
CONCLUSION: Our results demonstrated that rP21 may have adjuvant capacity able to modify the cytokine immune profile elicited by attenuated parasites. PMID: 31116244 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - May 24, 2019 Category: Infectious Diseases Authors: Pérez Brandán C, Mesias AC, Acuña L, Teixeira TL, da Silva CV Tags: Mem Inst Oswaldo Cruz Source Type: research

Lipase and factor V (but not viral load) are prognostic factors for the evolution of severe yellow fever cases.
CONCLUSIONS: YF acute severe cases show a generalised involvement of different organs (liver, spleen, heart, kidneys, intestines and pancreas), and different parameters were related to outcome. Factor V and lipase are independent variables associated with death, reinforcing the importance of hemorrhagic events due to fulminant liver failure and pointing to pancreatitis as a relevant event in the outcome of the disease. PMID: 31116245 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - May 24, 2019 Category: Infectious Diseases Authors: Casadio LVB, Salles APM, Malta FM, Leite GF, Ho YL, Gomes-Gouvêa MS, Malbouisson LMS, Levin AS, de Azevedo Neto RS, Carrilho FJ, Nastri ACSS, Pinho JRR Tags: Mem Inst Oswaldo Cruz Source Type: research

Mycobacterium genus and tRNA arrays.
Abstract The presence of tRNA array, a region with high tRNA gene number and density, has been demonstrated in Mycobacterium genus. However, a recent phylogenomic study revealed the existence of five distinct monophyletic groups (genera) within this genus. Considering this new scenario, and based on in-silico analyses, we have identified and characterised the abundance and diversity of tRNA array units within Mycobacterium, Mycolicibacterium gen. nov., Mycolicibacillus gen. nov., and Mycobacteroides gen. nov. The occurrence and prevalence of tRNA arrays among the genera belonging to Actinobacteria indicate their p...
Source: Memorias do Instituto Oswaldo Cruz - May 17, 2019 Category: Infectious Diseases Authors: Morgado SM, Vicente ACP Tags: Mem Inst Oswaldo Cruz Source Type: research

Therapeutic response and safety of the topical, sequential use of antiseptic, keratolytic, and pentamidine creams (3-PACK) on Leishmania (Viannia) braziliensis-infected mice.
bar P Abstract BACKGROUND: Topical treatment of New World cutaneous leishmaniasis can be affected by bacterial coinfection, hyperkeratosis, and transdermal drug delivery. OBJECTIVE: The aim of this work was to evaluate the therapeutic response and safety of the topical, sequential use of antiseptic, keratolytic, and pentamidine isethionate (PMD) creams (3-PACK kit) on CL-infected BALB/c mice. METHODS: A 0.5% chlorhexidine solution (CGH), 10% salicylic acid (SA), and 3% or 6% PMD were used as antiseptic, keratolytic, and antileishmanial drugs, respectively. During the first seven days, antiseptic, followe...
Source: Memorias do Instituto Oswaldo Cruz - May 17, 2019 Category: Infectious Diseases Authors: Muñoz BY, Mantilla JC, Escobar P Tags: Mem Inst Oswaldo Cruz Source Type: research

Novel molecular evidence of population structure in Anopheles (Kerteszia) bellator from Brazilian Atlantic Forest.
Abstract Anopheles bellator is a primary malaria vector in the Atlantic Forest. Partial sequences of timeless and Clock genes were used to assess the genetic differentiation of five Brazilian populations, which showed strong population structure (e.g. high F ST values and fixed differences) in all pairwise comparisons between Bahia sample and the others from Paraná, São Paulo and Rio de Janeiro states. Also, the resulting phylogenetic trees clearly grouped the sequences from Bahia in a different cluster with high bootstrap values. Among southern and southeastern populations low levels of genetic diff...
Source: Memorias do Instituto Oswaldo Cruz - May 17, 2019 Category: Infectious Diseases Authors: Voges K, da Rosa MPC, Westphal-Ferreira B, Navarro-Silva MA, Pontes CLM, Pitaluga AN, de Carvalho-Pinto CJ, Rona LD Tags: Mem Inst Oswaldo Cruz Source Type: research

Determination of the spatial susceptibility to Yellow Fever using a multicriteria analysis.
CONCLUSIONS: The multicriteria analysis revealed that the elements that were used are suited for and consistent in the prediction of the areas that are vulnerable to SYF. The results obtained indicate the proximity of the areas that are most vulnerable to the disease to densely populated areas where an Aedes aegypti infestation was observed, which confers a high risk of re-urbanisation of YF. PMID: 31066755 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - May 9, 2019 Category: Infectious Diseases Authors: de Paiva CA, Oliveira APS, Muniz SS, Calijuri ML, Dos Santos VJ, Alves SDC Tags: Mem Inst Oswaldo Cruz Source Type: research

Life cycle of International Congress of Parasitology (ICOPA).
PMID: 31066756 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - May 9, 2019 Category: Infectious Diseases Authors: Pikka J Tags: Mem Inst Oswaldo Cruz Source Type: research

Genomic basis of antibiotic resistance in Vibrio parahaemolyticus strain JPA1.
Abstract A multi-resistant strain of Vibrio parahaemolyticus was isolated from a tropical estuary in Rio de Janeiro, Brazil. Genome sequencing was conducted to establish the molecular basis of antibiotic resistance in this organism. The genetic content of this strain revealed it to be a non-virulent lineage that nevertheless possesses several antibiotic resistance determinants. PMID: 31038542 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - May 2, 2019 Category: Infectious Diseases Authors: Coutinho FH, Tschoeke DA, Clementino MM, Thompson CC, Thompson FL Tags: Mem Inst Oswaldo Cruz Source Type: research

Resistance to temephos and deltamethrin in Aedes aegypti from Brazil between 1985 and 2017.
CONCLUSIONS: (i) A robust dataset on the assessments of the insecticide resistance of Brazilian Ae. aegypti populations performed since 1985 was made available through our study. (ii) Our findings call into question the efficacy of chemical control as the sole methodology of vector control. (iii) It is necessary to ensure that sustainable insecticide resistance monitoring is maintained as a key component of integrated vector management. (iv) Consideration of additional parameters, beyond the supply of insecticides distributed by the MoH or the diverse local dynamics of dengue incidence, is necessary to find consistent corr...
Source: Memorias do Instituto Oswaldo Cruz - May 2, 2019 Category: Infectious Diseases Authors: Valle D, Bellinato DF, Viana-Medeiros PF, Lima JBP, Martins Junior AJ Tags: Mem Inst Oswaldo Cruz Source Type: research

Overview of artemisinin effectiveness during outset years of its implementation in the western Brazilian Amazon.
CONCLUSIONS: Artemisinin derivatives remained effective in the treatment of falciparum malaria during first 12-years of use in north area of Brazil. PMID: 31038549 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - May 2, 2019 Category: Infectious Diseases Authors: Pinto RM, Sampaio VS, de Melo GC, Alecrim MDGC, Mattos K, Perdomo RT, Cordeiro SDC, Parente AFA, de Carvalho LR, Mendes RP, Lacerda MVG, Monteiro WM, Weber SS Tags: Mem Inst Oswaldo Cruz Source Type: research

Combination of surveillance tools reveals that Yellow Fever virus can remain in the same Atlantic Forest area at least for three transmission seasons.
CONCLUSIONS: Collectively, our data revealed that YFV transmission persisted at the same Atlantic Forest area for at least three consecutive transmission seasons without the need of new introductions. Our real-time surveillance strategy permitted health authorities to take preventive actions within 48 h after the detection of the sick non-human primate. The local virus persistence and the proximity of the epizootic forest to urban areas reinforces the concern with regards to the risk of re-urbanisation and seasonal re-emergence of YFV, stressing the need for continuous effective surveillance and high vaccination coverage i...
Source: Memorias do Instituto Oswaldo Cruz - May 2, 2019 Category: Infectious Diseases Authors: Abreu FVS, Delatorre E, Dos Santos AAC, Ferreira-de-Brito A, de Castro MG, Ribeiro IP, Furtado ND, Vargas WP, Ribeiro MS, Meneguete P, Bonaldo MC, Bello G, Lourenço-de-Oliveira R Tags: Mem Inst Oswaldo Cruz Source Type: research

ERRATUM: Occurrence, antifungal susceptibility, and virulence factors of opportunistic yeasts isolated from Brazilian beaches.
Authors: Abstract [This corrects the article doi: 10.1590/0074-02760180566]. PMID: 30994734 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - April 18, 2019 Category: Infectious Diseases Tags: Mem Inst Oswaldo Cruz Source Type: research

Neutrophil CD64 expression levels in IGRA-positive individuals distinguish latent tuberculosis from active disease.
CONCLUSIONS: The positive regulation of CD64 expression in circulating neutrophils of patients with active TB could represent an additional biomarker for diagnosis of active TB and could be used for monitoring individuals with LTBI before progression of TB disease. PMID: 30970080 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - April 12, 2019 Category: Infectious Diseases Authors: Corrêa RDS, Rodrigues LS, Pereira LHL, Nogueira OC, Leung J, Sousa MDS, Hacker MA, Siqueira HR, Capone D, Alves RLR, Pessolani MCV, Schmitz V, Pereira GMB Tags: Mem Inst Oswaldo Cruz Source Type: research

Nanoemulsion containing essential oil from Xylopia ochrantha Mart. produces molluscicidal effects against different species of Biomphalaria (Schistosoma hosts).
CONCLUSION: Thus, these results suggest the use of nanoemulsified essential oil from X. ochrantha as a possible alternative in controlling some Biomphalaria species involved in the schistosomiasis cycle. PMID: 30970079 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - April 8, 2019 Category: Infectious Diseases Authors: Araújo FP, Albuquerque RDDG, Rangel LDS, Caldas GR, Tietbohl LAC, Santos MG, Ricci-Júnior E, Thiengo S, Fernandez MA, Santos JAAD, Faria RX, Rocha L Tags: Mem Inst Oswaldo Cruz Source Type: research

Human sapovirus GI.2 and GI.3 from children with acute gastroenteritis in northern Brazil.
a ACD Abstract Human sapoviruses (HSaV) are considered important causative agents of acute gastroenteritis in humans worldwide. However, knowledge of the genetic characteristics of the whole genome of HSaV in Brazil is limited. Here we report the complete genome sequences of six HSaVs GI.2 and two GI.3 strains obtained from children with acute gastroenteritis in the Northern region of Brazil. Next generation sequencing was used to obtain the full genome and molecular characterization of the genome was performed. Phylogenetic analysis of the genome was also performed. Only one complete HSaV GI.2 genome characteriza...
Source: Memorias do Instituto Oswaldo Cruz - April 4, 2019 Category: Infectious Diseases Authors: Cilli A, Luchs A, Leal E, Gill D, Milagres FAP, Komninakis SV, Brustulin R, Teles MDAR, Lobato MCABS, Chagas RTD, Abrão MFNDS, Soares CVDA, Deng X, Delwart E, Sabino EC, Costa ACD Tags: Mem Inst Oswaldo Cruz Source Type: research

Diagnosis of Schistosoma mansoni infections: what are the choices in Brazilian low-endemic areas?
Abstract The population of Brazil is currently characterised by many individuals harbouring low-intensity Schistosoma mansoni infections. The Kato-Katz technique is the diagnostic method recommended by the World Health Organization (WHO) to assess these infections, but this method is not sensitive enough in the context of low egg excretion. In this regard, potential alternatives are being employed to overcome the limits of the Kato-Katz technique. In the present review, we evaluated the performance of parasitological and immunological approaches adopted in Brazilian areas. Currently, the diagnostic choices involve...
Source: Memorias do Instituto Oswaldo Cruz - March 28, 2019 Category: Infectious Diseases Authors: Silva-Moraes V, Shollenberger LM, Siqueira LMV, Castro-Borges W, Harn DA, Grenfell RFQE, Rabello ALT, Coelho PMZ Tags: Mem Inst Oswaldo Cruz Source Type: research

Evaluation of insecticide resistance in Aedes aegypti populations connected by roads and rivers: the case of Tocantins state in Brazil.
CONCLUSIONS: With the exception of Palmas, the towns with the highest indexes (ovitrap positivity, number and density of eggs), as well with high levels of resistance and kdr alleles were located along the BR-153 road, indicating that the flow of people and cargo can contribute to the dispersion of the vector and potentially resistance. This study contributes substantially to knowledge regarding the insecticide resistance profile of Tocantins mosquito populations; the data generated via the study could facilitate the judicious use of insecticides by vector control programs. PMID: 30916115 [PubMed - in process] (Source...
Source: Memorias do Instituto Oswaldo Cruz - March 25, 2019 Category: Infectious Diseases Authors: Sá ELR, Rodovalho CM, Sousa NPR, Sá ILR, Bellinato DF, Dias LDS, Silva LCD, Martins AJ, Lima JBP Tags: Mem Inst Oswaldo Cruz Source Type: research

Protein-protein interactions of the nicotinamide/nicotinate mononucleotide adenylyltransferase of Leishmania braziliensis.
CONCLUSION: We constructed a model of PPI of LbNMNAT and showed its association with proteins involved in various functions such as protein folding, redox homeostasis, translation, and NAD synthesis. PMID: 30916117 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - March 21, 2019 Category: Infectious Diseases Authors: Ortiz-Joya L, Contreras-Rodríguez LE, Ramírez-Hernández MH Tags: Mem Inst Oswaldo Cruz Source Type: research

Molecular characterisation of the emerging measles virus from Roraima state, Brazil, 2018.
In this study, we report the complete genome sequencing of one measles virus isolate, genotype D8, that was obtained directly from a urine sample in Boa Vista city, the capital of Roraima state in Brazil. Phylogenetic reconstruction grouped the genome described in this study with that of samples from Australia, South Korea, and Italy. To our knowledge, this is the first complete genome sequence of a wild-type measles virus reported from Latin America. Therefore, the present data strengthen the current knowledge on the molecular epidemiology of measles worldwide. PMID: 30892375 [PubMed - in process] (Source: Memorias d...
Source: Memorias do Instituto Oswaldo Cruz - March 18, 2019 Category: Infectious Diseases Authors: Meneses CAR, Nascimento VAD, Souza VC, Maito RM, Gomes MA, Cunha CRSB, Antony IA, Silva MEOE, Campos DPS, Corado ALG, Pessoa KP, Monteiro DCDS, Okumoto O, Cunha MC, Pacheco FC, Naveca FG Tags: Mem Inst Oswaldo Cruz Source Type: research

Occurrence, antifungal susceptibility, and virulence factors of opportunistic yeasts isolated from Brazilian beaches.
CONCLUSIONS: The identification of opportunistic yeast species in seawater and sand samples from Brazilian beaches suggest a potential risk to the health of people who use these environments for recreational purposes. PMID: 30892381 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - March 14, 2019 Category: Infectious Diseases Authors: Maciel NO, Johann S, Brandão LR, Kucharíková S, Morais CG, Oliveira AP, Freitas GJ, Borelli BM, Pellizzari FM, Santos DA, Dijck PV, Rosa CA Tags: Mem Inst Oswaldo Cruz Source Type: research

Immunogenicity and safety of the combined vaccine for measles, mumps, and rubella isolated or combined with the varicella component administered at 3-month intervals: randomised study.
CONCLUSION: The demonstration of batch consistency and non-inferiority of the Bio-MMR vaccine completed the technology transfer. This is a significant technological achievement with implications for immunisation programs. PMID: 30843921 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - March 7, 2019 Category: Infectious Diseases Authors: Santos EMD, Noronha TG, Alves IS, Cruz RLS, Ferroco CLV, Brum RC, Oliveira PMN, Siqueira MM, Lima MC, Ramos FLP, Bragagnolo CM, Camacho LAB, Maia MLS Tags: Mem Inst Oswaldo Cruz Source Type: research

A localized outbreak of Chikungunya virus in Salvador, Bahia, Brazil.
Abstract A localized Chikungunya virus (CHIKV; East/Central/South African genotype) outbreak (50 cases, 70% laboratory-confirmed; attack rate: 5.3 confirmed cases/100 people) occurred in a Salvador, Brazil neighborhood, between Apr-Jun/2017. Highly clustered cases in space and time, mostly along a single street, highlight an increased risk of CHIKV transmission among pockets of susceptible populations. This finding underscores the need for ongoing local level surveillance for arboviral outbreaks. PMID: 30843962 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - February 28, 2019 Category: Infectious Diseases Authors: Tauro LB, Cardoso CW, Souza RL, Nascimento LC, Santos DRD, Campos GS, Sardi S, Reis OBD, Reis MG, Kitron U, Ribeiro GS Tags: Mem Inst Oswaldo Cruz Source Type: research

Brazilian malaria molecular targets (BraMMT): selected receptors for virtual high-throughput screening experiments.
CONCLUSIONS: The BraMMT is available to perform vHTS experiments using OCTOPUS or Raccoon software to improve the search for new antimalarial compounds. It can be retrieved from www.drugdiscovery.com.br or download of Supplementary data. PMID: 30810604 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - February 25, 2019 Category: Infectious Diseases Authors: Nunes RR, Fonseca ALD, Pinto ACS, Maia EHB, Silva AMD, Varotti FP, Taranto AG Tags: Mem Inst Oswaldo Cruz Source Type: research

Tuberculosis-HIV treatment with rifampicin or rifabutin: are the outcomes different?
Abstract BACKGROUND: Rifamycins are a group of antibiotics mainly used in the treatment of tuberculosis (TB), however they interact with antiretroviral therapy (ART). Rifabutin allows more regimens options for concomitant imunodeficiency virus (HIV) treatment compared to rifampicin. OBJECTIVE: Compare the outcomes of TB-HIV co-infected patients who used rifampicin or rifabutin. METHODS: We analysed data from a prospective cohort study at National Institute of Infectious Diseases Evandro Chagas, Rio de Janeiro (RJ), Brazil. Patients who were treated for TB and HIV with rifampicin or rifabutin, from Februa...
Source: Memorias do Instituto Oswaldo Cruz - February 11, 2019 Category: Infectious Diseases Authors: Schmaltz CAS, Demitto FO, Sant'Anna FM, Rolla VC Tags: Mem Inst Oswaldo Cruz Source Type: research

Detection and clearance of a mosquito densovirus contaminant from laboratory stocks of Zika virus.
CONCLUSIONS: Since the putative impact of viral contaminants in ZIKV strains generally used for research purposes is unknown, researchers working in the field must be aware of potential contaminants and test viral stocks to certify sample purity. PMID: 30758394 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - February 7, 2019 Category: Infectious Diseases Authors: Cataneo AHD, Kuczera D, Mosimann ALP, Silva EG, Ferreira ÁGA, Marques JT, Wowk PF, Santos CNDD, Bordignon J Tags: Mem Inst Oswaldo Cruz Source Type: research

Evaluation of three recombinant proteins for the development of ELISA and immunochromatographic tests for visceral leishmaniasis serodiagnosis.
CONCLUSIONS : The three antigens selected by us are promising candidates for VL diagnosis regardless of the test format, although the antigen combinations and test parameters may warrant further optimisation. PMID: 30726344 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - February 4, 2019 Category: Infectious Diseases Authors: Santos ARRD, Serufo ÂV, Figueiredo MM, Godoi LC, Vitório JG, Marcelino AP, Avelar DM, Rodrigues FTG, Machado-Coelho GLL, Medeiros FAC, Jerônimo SMB, Oliveira EJ, Nascimento FC, Teixeira SMR, Gazzinelli RT, Nagem RAP, Fernandes AP Tags: Mem Inst Oswaldo Cruz Source Type: research

Lack of quadruple and quintuple mutant alleles associated with sulfadoxine-pyrimethamine resistance in Plasmodium vivax isolates from Brazilian endemic areas.
CONCLUSION: No evidence of molecular signatures associated with P. vivax resistance to SP was observed in the Brazilian samples. PMID: 30726345 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - February 4, 2019 Category: Infectious Diseases Authors: Gomes LR, Lavigne A, Brasil P, Peterka CL, Ménard D, Daniel-Ribeiro CT, Ferreira-da-Cruz MF Tags: Mem Inst Oswaldo Cruz Source Type: research

Analytical validation of real-time quantitative PCR assays for optimum diagnosis of vivax malaria.
CONCLUSION: Real-time PCR may replace conventional PCR in reference laboratories for P. vivax detection due to its rapidity. The TaqMan™ system is the most indicated when quantification assays are required. Performing tests in triplicate when diagnosing Plasmodium-infected-asymptomatic individuals is recommended to minimise diagnostic errors. PMID: 30726341 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - January 31, 2019 Category: Infectious Diseases Authors: Almeida-de-Oliveira NK, Moreira OC, de Lavigne AR, Mendonça-Lima L, Werneck GL, Daniel-Ribeiro CT, Ferreira-da-Cruz MF Tags: Mem Inst Oswaldo Cruz Source Type: research

Drug development for cryptococcosis treatment: what can patents tell us?
CONCLUSION: The low number of patents in this field indicates that efforts to mitigate the unmet needs for cryptococcosis treatment remain insufficient. Without investment to drive research and innovation, patients will likely continue to face inadequate assistance. Given the current scenario characterised by poor funding and low interest for technological development, drug repurposing may be the best alternative for cryptococcosis treatment. PMID: 30726342 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - January 31, 2019 Category: Infectious Diseases Authors: Santos-Gandelman J, Machado-Silva A Tags: Mem Inst Oswaldo Cruz Source Type: research

Improving the reference standard for the diagnosis of canine visceral leishmaniasis: a challenge for current and future tests.
CONCLUSION: We concluded that improving the sensitivity of serum testing for diagnosing asymptomatic dogs must constitute a priority in the process of developing new diagnostic tests to be used in the visceral leishmaniasis control program in Brazil. PMID: 30726343 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - January 31, 2019 Category: Infectious Diseases Authors: Teixeira AIP, Silva DM, Vital T, Nitz N, de Carvalho BC, Hecht M, Oliveira D, Oliveira E, Rabello A, Romero GAS Tags: Mem Inst Oswaldo Cruz Source Type: research

First isolation of West Nile virus in Brazil.
CONCLUSIONS: Here we report the first isolation of WNV in Brazil from a horse with neurologic disease, which was clustered into lineage 1a with others US WNV strains isolated in beginning of 2000's decade. PMID: 30672980 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - January 17, 2019 Category: Infectious Diseases Authors: Martins LC, Silva EVPD, Casseb LMN, Silva SPD, Cruz ACR, Pantoja JAS, Medeiros DBA, Martins Filho AJ, Cruz EDRMD, Araújo MTF, Cardoso JF, Cunha MACRD, Almada GL, Romano APM, Santos MGDP, Rodrigues GAP, Chiang JO, Quaresma JAS, Carvalho VL, Vasconcelos PF Tags: Mem Inst Oswaldo Cruz Source Type: research

The cost of a disease targeted for elimination in Brazil: the case of schistosomiasis mansoni.
CONCLUSIONS: The economic burden of schistosomiasis mansoni in Brazil is high and results in the loss of productivity. Its persistence in Brazil is a challenge to public health and requires inter-sectorial interventions in areas such as indoor water supply, basic sanitation, and education. PMID: 30652735 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - January 14, 2019 Category: Infectious Diseases Authors: Nascimento GL, Pegado HM, Domingues ALC, Ximenes RAA, Itria A, Cruz LN, Oliveira MRF Tags: Mem Inst Oswaldo Cruz Source Type: research

Origin, tempo, and mode of the spread of DENV-4 Genotype IIB across the state of S ão Paulo, Brazil during the 2012-2013 outbreak.
CONCLUSIONS: The spread pattern of DENV-4 Genotype IIB strains across the country supports two independent introductions of the virus in São Paulo in a short period of time. Furthermore, São Paulo appears to have played a pivotal role in the dissemination of DENV-4 to other locations in Brazil. PMID: 30624458 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - January 7, 2019 Category: Infectious Diseases Authors: Ortiz-Baez AS, Cunha MDP, Vedovello D, Colombo TE, Nogueira ML, Villabona-Arenas CJ, Zanotto PMA Tags: Mem Inst Oswaldo Cruz Source Type: research

Exploring tRNA gene cluster in archaea.
CONCLUSIONS: tRNA gene cluster occurs in the three domains of life, suggesting a role of this type of tRNA gene organisation in the biology of the living organisms. PMID: 30624459 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - January 7, 2019 Category: Infectious Diseases Authors: Morgado SM, Vicente ACP Tags: Mem Inst Oswaldo Cruz Source Type: research

Rodent-borne viruses survey in rural settlers from Central Brazil.
This study aimed to investigate the prevalence of rodent-borne virus infections in seven rural settlements from Midwestern Brazil. Of the 466 individuals tested 12 (2.57%) were reactive for orthohantavirus and 3 (0.64%) for mammarenavirus. These rural settlers lived under unfavorable infrastructure, socioeconomic disadvantages, and unsanitary conditions, representing a risk for rodent-borne infections. Development of public policies towards the improvement of health, sanitation and awareness of rodent-borne diseases in improvised camps and settlements is imperative, in order to reduce morbidity and mortality caused by thes...
Source: Memorias do Instituto Oswaldo Cruz - December 17, 2018 Category: Infectious Diseases Authors: Fernandes J, Oliveira RC, Coelho TA, Martins RMB, Caetano KAA, Horta MAP, Levis S, Carneiro MADS, Teles SA, Lemos ERS Tags: Mem Inst Oswaldo Cruz Source Type: research

Methylation of p16 ink4a promoter is independent of human papillomavirus DNA physical state: a comparison between cervical pre-neoplastic and neoplastic samples.
CONCLUSIONS There was an increase in methylation of the p16 ink4a promoter from pre-neoplastic lesions to cancer. Additionally, a high frequency of E1/E2 disruptions in LSIL/HSIL suggested that viral DNA integration was an early event in cervical disease. Moreover, the methylation status was apparently independent of HPV16 integrity. PMID: 30569945 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - December 17, 2018 Category: Infectious Diseases Authors: Carestiato FN, Amaro-Filho SM, Moreira MAM, Cavalcanti SMB Tags: Mem Inst Oswaldo Cruz Source Type: research

Complete and de novo assembly of the Leishmania braziliensis (M2904) genome.
a/ JM Abstract Leishmania braziliensis is the etiological agent of American mucosal leishmaniasis, one of the most severe clinical forms of leishmaniasis. Here, we report the assembly of the L. braziliensis (M2904) genome into 35 continuous chromosomes. Also, the annotation of 8395 genes is provided. The public availability of this information will contribute to a better knowledge of this pathogen and help in the search for vaccines and novel drug targets aimed to control the disease caused by this Leishmania species. PMID: 30540030 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - December 10, 2018 Category: Infectious Diseases Authors: Fuente SG, Camacho E, Peiró-Pastor R, Rastrojo A, Carrasco-Ramiro F, Aguado B, Requena/ JM Tags: Mem Inst Oswaldo Cruz Source Type: research

Importance of cerebrospinal fluid investigation during dengue infection in Brazilian Amazonia Region.
CONCLUSIONS We found intense inflammatory CSF that is unusual in CNS disorders caused by dengue infection. It may be due co-infections or the immunogenetic background of the local Amerindian Brazilian population. CSF examination is an important diagnostic support tool for neurological dengue diagnosis. PMID: 30540031 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - December 10, 2018 Category: Infectious Diseases Authors: Bastos MS, Martins VDCA, Silva NLD, Jezine S, Pinto S, Aprigio V, Monte RL, Fragoso S, Puccioni-Sohler M Tags: Mem Inst Oswaldo Cruz Source Type: research