Characterising ISWI chromatin remodeler in Trypanosoma cruzi.
CONCLUSIONS In addition to its participation in transcriptional silencing, as it was reported in T. brucei, the data generated here provide a framework that suggests a role for TcISWI chromatin remodeler in different nuclear processes in T. cruzi, including mRNA nuclear export control and chromatin compaction. Further work is necessary to clarify the TcISWI functional diversity that arises from this protein interaction study. PMID: 32428081 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - May 21, 2020 Category: Infectious Diseases Authors: Díaz-Olmos Y, Batista M, Ludwig A, Marchini FK Tags: Mem Inst Oswaldo Cruz Source Type: research

Influenza A(H1N1)pdm09 infection and viral load analysis in patients with different clinical presentations.
This study aimed to evaluate the rate of infection and the role of influenza VL on the clinical spectrum of illnesses among different patient groups attended at a tertiary hospital in Brazil. METHODS Samples were collected from patients presenting acute respiratory infection from 2009 to 2013. Overall, 2262 samples were analysed and distributed into three groups: (i) asymptomatic (AS); (ii) symptomatic outpatients (OP); and (iii) hospitalised patients (HP). VL (expressed in Log10 RNA copies/mL) was calculated through a quantitative real-time one-step reverse transcription-polymerase chain reaction (RT-PCR) assay aimed at t...
Source: Memorias do Instituto Oswaldo Cruz - May 21, 2020 Category: Infectious Diseases Authors: Rodrigues Guimarães Alves V, Perosa AH, de Souza Luna LK, Cruz JS, Conte DD, Bellei N Tags: Mem Inst Oswaldo Cruz Source Type: research

Infestation of an endemic arbovirus area by sympatric populations of Aedes aegypti and Aedes albopictus in Brazil.
This study aimed to investigate and compare the infestation pattern of these species in a neighbourhood of Recife, Brazil, endemic for arboviruses in 2005 (T1) and 2013 (T2). METHODS Infestation, distribution and relative abundance of these sympatric species were recorded by egg collection using a network of 59 sentinel ovitraps (s-ovt) at fixed sampling stations for 12 months in T1 and T2. FINDINGS A permanent occupation pattern was detected which was characterised by the presence of egg-laying females of one or both species with a high ovitrap positivity index (94.3 to 100%) throughout both years analysed. In terms of ab...
Source: Memorias do Instituto Oswaldo Cruz - May 21, 2020 Category: Infectious Diseases Authors: Barbosa RMR, Melo-Santos MAV, Silveira JC, Silva-Filha MHNL, Souza WV, Oliveira CMF, Ayres CFJ, Xavier MDN, Rodrigues MP, Santos SAD, Nakazawa MM, Regis LN Tags: Mem Inst Oswaldo Cruz Source Type: research

Characterisation of alternative expression vectors for recombinant Bacillus Calmette-Gu érin as live bacterial delivery systems.
CONCLUSION We have obtained a set of potentially stable mycobacterial vectors with a arrange of expression levels, to be used in the development of rBCG vaccines. PMID: 32428188 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - May 21, 2020 Category: Infectious Diseases Authors: Nascimento LV, Santos CC, Leite LC, Nascimento IP Tags: Mem Inst Oswaldo Cruz Source Type: research

Genomic detection of a virus lineage replacement event of dengue virus serotype 2 in Brazil, 2019.
CONCLUSIONS Our study describes the early detection of a newly introduced and rapidly-expanding DENV2 virus lineage in Brazil. PMID: 32428189 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - May 21, 2020 Category: Infectious Diseases Authors: de Jesus JG, Dutra KR, Sales FCDS, Claro IM, Terzian AC, Candido DDS, Hill SC, Thézé J, Torres C, D'Agostini TL, Felix AC, Reis AFN, Alcantara LCJ, de Abreu AL, Croda JH, de Oliveira WK, de Filipis AMB, Camis MDCRDS, Romano CM Tags: Mem Inst Oswaldo Cruz Source Type: research

Schistosoma mansoni granulomas in the skeletal striated muscles in the murine model of neuroschistosomiasis: histological findings.
We describe here, for the first time in the literature, injuries in the skeletal musculature due to Schistosoma mansoni nfection. PMID: 32401896 [PubMed - as supplied by publisher] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - May 14, 2020 Category: Infectious Diseases Authors: Fidelis TAA, Brasileiro-Filho G, Parreiras PM, Coelho PMZ, Araujo N, Chaud MV, Baldo DA, Dos Santos NB, Lambertucci JR Tags: Mem Inst Oswaldo Cruz Source Type: research

Newly sequenced genomes of four Bacillus Calmette Guerin vaccines.
ys PN Abstract Bacillus Calmette Guerin (BCG) vaccines comprise a family of related strains. Whole genome sequencing has allowed the better characterisation of the differences between many of the BCG vaccines. As sequencing technologies improve, updating of publicly available sequence data becomes common practice. We hereby announce the draft genome of four commonly used BCG vaccines in Brazil, Argentina and Venezuela. PMID: 32401897 [PubMed - as supplied by publisher] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - May 14, 2020 Category: Infectious Diseases Authors: Sisco MC, Silva MG, Lopez B, Arguelles C, Mendonça-Lima L, de Waard JH, Duarte RS, Suffys PN Tags: Mem Inst Oswaldo Cruz Source Type: research

Frequency of first and second-line drug resistance-associated mutations among resistant Mycobacterium tuberculosis clinical isolates from S ão Paulo, Brazil.
CONCLUSIONS The present study is the first to report the frequency of mutations related to second-line drug resistance in MDR-TB, pre-XDR-TB and XDR-TB isolates. The results could lead to the improvement of available technologies for the rapid detection of drug resistant TB. PMID: 32401997 [PubMed - as supplied by publisher] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - May 14, 2020 Category: Infectious Diseases Authors: Matsui T, Pinhata JMW, Rabello MCDS, Brandão AP, Ferrazoli L, Leão SC, Viana-Niero C, Oliveira RS Tags: Mem Inst Oswaldo Cruz Source Type: research

Computational prediction and characterisation of miRNAs and their pathway genes in human schistosomiasis caused by Schistosoma haematobium.
CONCLUSIONS miRNA pathway occurs in the S. haematobium, including endogenous miRNAs and miRNA pathway components, suggesting a role of this type of non-coding RNAs in gene regulation in the parasite. The results found in this work will open up a new avenue for studying miRNAs in the S. haematobium biology in helping to understand the mechanism of gene silencing in the human parasite Schistosome. PMID: 32401998 [PubMed - as supplied by publisher] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - May 14, 2020 Category: Infectious Diseases Authors: Cardoso TCS, de Araújo CB, Portilho LG, Mendes LGA, Alves TC, Silva GC, Ribeiro THC, Gandolfi PE, Morais ER, do Amaral LR, Gomes MS Tags: Mem Inst Oswaldo Cruz Source Type: research

Aedes aegypti spreading in South America: new coldest and southernmost records.
Abstract The geographic distribution of Aedes (Stegomyia) aegypti (L.) in South America has been expanding during the last decades. Herein we present two new distribution records that extend its southern limits towards localities with extremer environmental conditions than reported to date. San Antonio Oeste constitutes the southernmost finding for the continent (40º44'S), whereas Tandil is the infested locality with the coldest mean annual temperature in Argentina (14.17ºC). The projection of a previous distribution model for Ae. aegypti predicts these two cities as positive and suggests several other l...
Source: Memorias do Instituto Oswaldo Cruz - May 14, 2020 Category: Infectious Diseases Authors: Rubio A, Cardo MV, Vezzani D, Carbajo AE Tags: Mem Inst Oswaldo Cruz Source Type: research

A cross-sectional approach including dog owner characteristics as predictors of visceral leishmaniasis infection in dogs.
CONCLUSION: This is the first study that relates higher socioeconomic status of dog owners as an independent factor associated with higher prevalence of VL infection, along with other strongly associated factors related to receptive environment for phlebotomines. Our findings strengthen the need for exploration of the biological and behavioural bases linking dog owner characteristics to the risk of canine infection in prospective cohort studies. PMID: 32348406 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - May 1, 2020 Category: Infectious Diseases Authors: Teixeira AIP, Silva DM, de Freitas LRS, Romero GAS Tags: Mem Inst Oswaldo Cruz Source Type: research

Minor temperature shifts do not affect chromosomal ploidy but cause transcriptomic changes in Leishmania braziliensis promastigotes in vitro.
CONCLUSIONS: This study provides evidence that L. braziliensis promastigotes exhibit a decrease in cell density, and noticeable changes in the transcriptomic profiles. However, there were not perceptible changes at chromosome CNVs and only ~3% of the genes changed their copies in each treatment. PMID: 32348407 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - May 1, 2020 Category: Infectious Diseases Authors: Ballesteros N, Vásquez NM, Patiño LH, Cruz-Saavedra L, Ramírez JD Tags: Mem Inst Oswaldo Cruz Source Type: research

DNA nanovaccines prepared using LemA antigen protect Golden Syrian hamsters against Leptospira lethal infection.
Abstract BACKGROUND: Nanoparticles (NPs) are viable candidates as carriers of exogenous materials into cells via transfection and can be used in the DNA vaccination strategy against leptospirosis. OBJECTIVES: We evaluated the efficiency of halloysite clay nanotubes (HNTs) and amine-functionalised multi-walled carbon nanotubes (NH2-MWCNTs) in facilitating recombinant LemA antigen (rLemA) expression and protecting Golden Syrian hamsters (Mesocricetus auratus) against Leptospira interrogans lethal infection. METHODS: An indirect immunofluorescent technique was used to investigate the potency of HNTs and NH2...
Source: Memorias do Instituto Oswaldo Cruz - April 24, 2020 Category: Infectious Diseases Authors: Oliveira TL, Bacelo KL, Forster KM, Ilha V, Rodrigues OE, Hartwig DD Tags: Mem Inst Oswaldo Cruz Source Type: research

Rapid detection of Mycobacterium tuberculosis DNA and genetic markers for Isoniazid resistance in Ziehl-Neelsen stained slides.
CONCLUSIONS: qPCR genotyping proved to be an efficient method to detect genes that confer M. tuberculosis resistance to INH. Thus, qPCR genotyping may be an alternative instead of sequencing. PMID: 32321155 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - April 24, 2020 Category: Infectious Diseases Authors: Bello GL, Morais FCL, de Jesus SP, Wolf JM, Gehlen M, de Almeida IN, Figueiredo LJA, Soares TDS, Barcellos RB, Dalla Costa ER, de Miranda SS, Rossetti MLR Tags: Mem Inst Oswaldo Cruz Source Type: research

Leishmania infantum induces high phagocytic capacity and intracellular nitric oxide production by human proinflammatory monocyte.
CONCLUSIONS: Our results showed that L. braziliensis and L. infantum induce different innate immune responses and a highly inflammatory profile, which is characteristic of infection by L. infantum, the species associated with visceral disease. PMID: 32321156 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - April 24, 2020 Category: Infectious Diseases Authors: Ribeiro CV, Rocha BFB, Oliveira E, Teixeira-Carvalho A, Martins-Filho OA, Murta SMF, Peruhype-Magalhães V Tags: Mem Inst Oswaldo Cruz Source Type: research

Mapping the tuberculosis scientific landscape among BRICS countries: a bibliometric and network analysis.
CONCLUSIONS: An overview of BRICS scientific publications and interactions highlighted the necessity to develop a BRICS TB research plan to increase efforts and funding to ensure that basic science research successfully translates into products and policies to help end the TB epidemic. PMID: 32187325 [PubMed - as supplied by publisher] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - March 20, 2020 Category: Infectious Diseases Authors: Castor K, Mota FB, da Silva RM, Cabral BP, Maciel EL, de Almeida IN, Arakaki-Sanchez D, Andrade KB, Testov V, Vasilyeva I, Zhao Y, Zhang H, Singh M, Rao R, Tripathy S, Gray G, Padayatchi N, Bhagwandin N, Swaminathan S, Kasaeva T, Kritski A Tags: Mem Inst Oswaldo Cruz Source Type: research

Involvement of lipid microdomains in human endothelial cells infected by Streptococcus agalactiae type III belonging to the hypervirulent ST-17.
CONCLUSIONS: The collection of our results suggests that lipid microdomain affects the interaction of S. agalactiae type III belonging to the hypervirulent ST-17 with HUVEC cells through PI3K/Akt signaling pathway. PMID: 32187326 [PubMed - as supplied by publisher] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - March 20, 2020 Category: Infectious Diseases Authors: Ferreira BJ, Lannes-Costa PS, Santos GDS, Mermelstein C, Einicker-Lamas M, Nagao PE Tags: Mem Inst Oswaldo Cruz Source Type: research

Human papillomavirus type 18 E5 oncoprotein cooperates with E6 and E7 in promoting cell viability and invasion and in modulating the cellular redox state.
CONCLUSIONS: This study demonstrates for the first time that HPV-18 E5, E6, and E7 oncoproteins can cooperate to enhance malignant transformation. PMID: 32187327 [PubMed - as supplied by publisher] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - March 20, 2020 Category: Infectious Diseases Authors: Hochmann J, Parietti F, Martínez J, Lopez AC, Carreño M, Quijano C, Boccardo E, Sichero L, Möller MN, Mirazo S, Arbiza J Tags: Mem Inst Oswaldo Cruz Source Type: research

Phylogenetics applied to the human immunodeficiency virus type 1 (HIV-1): from the cross-species transmissions to the contact network inferences.
o G Abstract Phylogenetic analyses were crucial to elucidate the origin and spread of the pandemic human immunodeficiency virus type 1 (HIV-1) group M virus, both during the pre-epidemic period of cryptic dissemination in human populations as well as during the epidemic phase of spread. The use of phylogenetics and phylodynamics approaches has provided important insights to track the founder events that resulted in the spread of HIV-1 strains across vast geographic areas, specific countries and within geographically restricted communities. In the recent years, the use of phylogenetic analysis combined with the hug...
Source: Memorias do Instituto Oswaldo Cruz - March 20, 2020 Category: Infectious Diseases Authors: Gräf T, Delatorre E, Bello G Tags: Mem Inst Oswaldo Cruz Source Type: research

A new gene inventory of the ubiquitin and ubiquitin-like conjugation pathways in Giardia intestinalis.
Abstract BACKGROUND: Ubiquitin (Ub) and Ub-like proteins (Ub-L) are critical regulators of complex cellular processes such as the cell cycle, DNA repair, transcription, chromatin remodeling, signal translation, and protein degradation. Giardia intestinalis possesses an experimentally proven Ub-conjugation system; however, a limited number of enzymes involved in this process were identified using basic local alignment search tool (BLAST). This is due to the limitations of BLAST's ability to identify homologous functional regions when similarity between the sequences dips to
Source: Memorias do Instituto Oswaldo Cruz - March 5, 2020 Category: Infectious Diseases Authors: Castellanos IC, Calvo EP, Wasserman M Tags: Mem Inst Oswaldo Cruz Source Type: research

Effects of El Ni ño-Southern Oscillation on human visceral leishmaniasis in the Brazilian State of Mato Grosso do Sul.
CONCLUSIONS: The results demonstrated that the interannual cycles of incidence of human VL are significantly associated with the occurrence of the ENSO phenomenon and its phases, El Niño and La Niña. PMID: 32130366 [PubMed - as supplied by publisher] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - March 5, 2020 Category: Infectious Diseases Authors: Silva Neto ABD, Oliveira EF, Encina CCC, Figueiredo HR, Paranhos Filho AC, Oliveira AG Tags: Mem Inst Oswaldo Cruz Source Type: research

Expression of NLRP3 inflammasome in leprosy indicates immune evasion of Mycobacterium leprae.
CONCLUSIONS: Our results demonstrate that the NLRP3 inflammasome is inactive in leprosy, suggesting immune evasion of M. leprae. PMID: 32130367 [PubMed - as supplied by publisher] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - March 5, 2020 Category: Infectious Diseases Authors: Mendes ALG, Joaquim HDM, Zamae MIS, Assis RM, Peixoto JRM, de Araújo MMG, Guedes ACM, Oliveira EJ, Magalhães VP, Pascoal-Xavier MA Tags: Mem Inst Oswaldo Cruz Source Type: research

Oropouche virus detection in saliva and urine.
Abstract Oropouche virus (OROV) is an arthropod-borne virus of the Peribunyaviridae family, transmitted to humans primarily by Culicoides paraensis. It is one of the main arboviruses infecting humans in Brazil, primarily in the Amazon Region. Here, we report the detection of OROV in the saliva and urine of a patient whose samples were collected five days after the onset of symptoms. Nucleotide sequencing and phylogenetic analysis further confirmed the results. To our knowledge, this is the first study reporting the detection of OROV in the saliva and urine of an infected patient. In addition, the results of our st...
Source: Memorias do Instituto Oswaldo Cruz - March 5, 2020 Category: Infectious Diseases Authors: Nascimento VAD, Santos JHA, Monteiro DCDS, Pessoa KP, Cardoso AJL, Souza VC, Abdalla LF, Naveca FG Tags: Mem Inst Oswaldo Cruz Source Type: research

Glycogen synthase kinase 3 ß participates in late stages of Dengue virus-2 infection.
CONCLUSIONS: The results suggest that GSK3β participates at the late stages of the DENV replication cycle, where viral activation may promote apoptosis and release of viral particles. PMID: 32130369 [PubMed - as supplied by publisher] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - March 5, 2020 Category: Infectious Diseases Authors: Cuartas-López AM, Gallego-Gómez JC Tags: Mem Inst Oswaldo Cruz Source Type: research

FLI1 gene influences lesion size and skin test may predict therapeutic response in cutaneous leishmaniasis.
In this study, we examined whether the genes previously associated with CL influenced the clinical outcome. Patients were genotyped and retrospectively classified as responders, who were cured with a single course of pentavalent antimony (Sbv), or as refractories, who did not respond to Sbv. Patients characterised as responders showed a stronger response to the leishmanin skin test (LST) when compared to the refractory subjects (p = 0.0003). Furthermore, we observed an association between the FLI1 CC genotype and an increased size of ulcers (p = 0.0170). We suggest that the leishmanin skin test may be a predictive tool for...
Source: Memorias do Instituto Oswaldo Cruz - March 5, 2020 Category: Infectious Diseases Authors: da Hora AS, de Almeida LF, do Lago TS, Machado PR, Castellucci LC Tags: Mem Inst Oswaldo Cruz Source Type: research

Human acute Chagas disease: changes in factor VII, activated protein C and hepatic enzymes from patients of oral outbreaks in Par á State (Brazilian Amazon).
In conclusion, the present study raises the possibility that a disturbance in coagulation and hepatic function may be linked to human ACD. PMID: 32130371 [PubMed - as supplied by publisher] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - March 5, 2020 Category: Infectious Diseases Authors: Santos VRCD, Antunes D, Souza DDSM, Moreira OC, Lima ICA, Farias-de-Oliveira DA, Lobo JP, de Meis E, Coura JR, Savino W, Junqueira ACV, de Meis J Tags: Mem Inst Oswaldo Cruz Source Type: research

Antiparasitic and anti-inflammatory activities of ß-lapachone-derived naphthoimidazoles in experimental acute Trypanosoma cruzi infection.
CONCLUSION: N1 showed moderate trypanocidal and promising immunomodulatory activities, and its use in combination with benznidazole and/or anti-arrhythmic drugs as well as the efficacy of its alternative formulations must be investigated in the near future. PMID: 32074167 [PubMed - as supplied by publisher] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - February 20, 2020 Category: Infectious Diseases Authors: Cascabulho CM, Meuser-Batista M, Moura KCG, Pinto MDC, Duque TLA, Demarque KC, Guimarães ACR, Manso PPA, Pelajo-Machado M, Oliveira GM, Castro SL, Menna-Barreto RF Tags: Mem Inst Oswaldo Cruz Source Type: research

Increasing putative vector importance of Trichophoromyia phlebotomines (Diptera: Psychodidae).
Abstract Despite some phlebotomines being well recognised as vectors of leishmaniasis agents, vector importance of those belonging to the genus Trichophoromyia has not been extensively studied. The present study provides evidence regarding the putative vector role played by some species of Trichophoromyia on leishmanine enzootics, based on literature reports and findings obtained from field experiments conducted in the ecotopes of Pará State, Brazil. The species Th. ubiquitalis, Th. velascoi, Th. auraensis, Th. ininii and Th. brachipyga possess minimal criteria to be included in the list of suspected leishm...
Source: Memorias do Instituto Oswaldo Cruz - February 14, 2020 Category: Infectious Diseases Authors: Santos TVD, Silveira FT Tags: Mem Inst Oswaldo Cruz Source Type: research

Characterisation of the in vitro activity of a Nitazoxanide-N-methyl-1H-benzimidazole hybrid molecule against albendazole and nitazoxanide susceptible and resistant strains of Giardia intestinalis and its in vivo giardicidal activity.
CONCLUSIONS: The in vitro and in vivo giardicidal activity of CMC-20 suggests its potential use in the treatment of giardiasis. PMID: 32049098 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - February 14, 2020 Category: Infectious Diseases Authors: Matadamas-Martínez F, Nogueda-Torres B, Castillo R, Hernández-Campos A, Barrera-Valdes ML, León-Ávila G, Hernández JM, Yépez-Mulia L Tags: Mem Inst Oswaldo Cruz Source Type: research

Assessment of Culex pipiens bioforms in the world's southernmost distribution limit.
Abstract The mosquito Culex pipiens s.s. L. occurs as two bioforms that differ in physiology and behaviour affecting virus transmission cycles. To assess the occurrence of Cx. pipiens bioforms in the southernmost limit of its distribution, specimens were collected aboveground in southern Buenos Aires Province and east Patagonia, Argentina. Ten larvae and 25 adults were individually processed and identified by polymerase chain reaction (PCR) amplification of Ace-2 and CQ11 loci. Culex quinquefasciatus Say (one larva, two adults), Cx. pipiens f. molestus (one larva, one adult) and one adult of hybrid origin were ide...
Source: Memorias do Instituto Oswaldo Cruz - February 14, 2020 Category: Infectious Diseases Authors: Cardo MV, Rubio A, Vezzani D, Carbajo AE Tags: Mem Inst Oswaldo Cruz Source Type: research

Gestation and breastfeeding in schistosomotic mice differentially alters the expression of histone deacetylases (HDACs) in adult offspring.
CONCLUSIONS: Breastfeeding induced the expression of HDACs from various classes involved in reducing inflammatory responses. However, gestation enhanced the expression of a single HDAC and breastfeeding or gestation appears to favour multiple IL-10-dependent pathways, but not cells with a regulatory phenotype. PMID: 32022099 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - February 7, 2020 Category: Infectious Diseases Authors: Holanda GCR, Souto FO, Silva MDC, Lorena VMB, Costa VMA, Albuquerque MCPA, Souza VMO, Lima Filho JL Tags: Mem Inst Oswaldo Cruz Source Type: research

Plasmodium infection and its association with biochemical and haematological parameters in free-living Alouatta guariba clamitans (Cabrera, 1940) (Primates: Atelidae) in Southern Brazil.
CONCLUSIONS: Therefore, Plasmodium infection in howler monkeys may causes haematological/biochemical alterations which might suggest hepatic impairment. Moreover, infection must be monitored for the eco-epidemiological surveillance of malaria in the Atlantic Forest and during primate conservation program that involves the animal movement, such as translocations. PMID: 32022168 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - February 7, 2020 Category: Infectious Diseases Authors: Nunes AJD, Alvarenga DAM, de Souza Junior JC, Peruchi AR, Gonçalves GHP, Hirano ZMB, Brito CFA, Cremer MJ Tags: Mem Inst Oswaldo Cruz Source Type: research

Iron intake is positively associated with viral load in antiretroviral na ïve Brazilian men living with HIV.
Iron intake is positively associated with viral load in antiretroviral naïve Brazilian men living with HIV. Mem Inst Oswaldo Cruz. 2020;114:e190350 Authors: Gonçalves JL, Silva MCA, Roma EH, Grinsztejn B, Lemos ADS, Gorni N, Cruz AM, Almeida CF, Quintana MSB, Bonecini-Almeida MDG, Brito PD Abstract BACKGROUND: Iron homeostasis contribute for the human immunodeficiency virus (HIV) pathogenesis. OBJECTIVES: We assessed the iron intake pattern in antiretroviral naïve Brazilian men living with HIV correlating with clinical and nutritional parameters. METHODS: The iron consumptio...
Source: Memorias do Instituto Oswaldo Cruz - February 7, 2020 Category: Infectious Diseases Authors: Gonçalves JL, Silva MCA, Roma EH, Grinsztejn B, Lemos ADS, Gorni N, Cruz AM, Almeida CF, Quintana MSB, Bonecini-Almeida MDG, Brito PD Tags: Mem Inst Oswaldo Cruz Source Type: research

Erythrocytes morphology and hemorheology in severe bacterial infection.
CONCLUSIONS: The inflammatory process triggered by bacterial infection induced pathological changes in RBCs and platelets activation. Treatment of infected animals with CpLP prevented the emergence of RBCs abnormal morphology and this may have implications in the protective effect of CpLP, avoiding animal death. PMID: 31859703 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - December 22, 2019 Category: Infectious Diseases Authors: Silva AF, Sousa JS, Cunha PL, Lima-Filho JV, Alencar NM, Freitas CD, Oliveira CL, Ramos MV Tags: Mem Inst Oswaldo Cruz Source Type: research

Extracellular vesicles isolated from Trypanosoma cruzi affect early parasite migration in the gut of Rhodnius prolixus but not in Triatoma infestans.
Abstract The protozoan Trypanosoma cruzi has the ability to spontaneously secrete extracellular vesicles (EVs). In this paper, T. cruzi EVs derived from epimastigote forms were evaluated during interaction with triatomine bugs Rhodnius prolixus and Triatoma infestans. T. cruzi EVs were purified and artificially offered to the insects prior to infection with epimastigote forms. No effect of EVs was detected in the parasite counts in the guts of both vectors after 49-50 days. On the other hand, pre-feeding with EVs delayed parasite migration to rectum only in the gut in R. prolixus after 21-22 days. Those data sugge...
Source: Memorias do Instituto Oswaldo Cruz - December 19, 2019 Category: Infectious Diseases Authors: Paranaiba LF, Guarneri AA, Torrecilhas AC, Melo MN, Soares RP Tags: Mem Inst Oswaldo Cruz Source Type: research

Generation of Yellow Fever virus vaccine in skeletal muscle cells of chicken embryos.
CONCLUSIONS: In conclusion, the primary culture of chicken skeletal muscle cells is a good model for studying muscle cells infected with YF17DD virus. This culture system displays satisfactory emulation of the in vitro phenomenon observed, contributing to our understanding of virus infection dynamics and leading to the development of alternative methods of vaccine production. PMID: 31826129 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - December 12, 2019 Category: Infectious Diseases Authors: Souza YRM, Manso PPA, Oliveira BCD, Terra MABL, Paschoal T, Caminha G, Ribeiro IP, Raphael LMS, Bonaldo MC, Pelajo-Machado M Tags: Mem Inst Oswaldo Cruz Source Type: research

Increasing the digital repository of DNA barcoding sequences of sand flies (Psychodidae: Phlebotominae).
This study incorporates barcode sequences, for the first time, for Evandromyia cortelezzii and Migonemyia migonei from Argentina. The nucleotide sequence divergences were estimated to generate a neighbour-joining (NJ) tree. The automatic barcode gap discovery (ABGD) approach was employed to find the barcode gaps and the operational taxonomic unit (OTU) delimitation. Other species of the subtribe were included. The frequency histogram of divergences showed a barcoding gap. The ABGD analysis identified 14 operational taxonomic units (OTUs) from 13 morphological species. Sequences of Ev. cortelezzii and Mg. migonei formed wel...
Source: Memorias do Instituto Oswaldo Cruz - December 6, 2019 Category: Infectious Diseases Authors: Laurito M, Ontivero IM, Almirón WR Tags: Mem Inst Oswaldo Cruz Source Type: research

Acinetobacter baumannii infections in Amazon Region driven by extensively drug resistant international clones, 2016-2018.
CONCLUSIONS: The A. baumannii epidemiology in Brazilian Amazon Region was unknown. It was demonstrated that A. baumannii XDR international clones were responsible for nosocomial infections in Boa Vista during 2016-2018, revealing that the epidemiological scenario of A. baumannii infections in Amazon Region resembles those from the cosmopolitan regions worldwide. PMID: 31778426 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - December 1, 2019 Category: Infectious Diseases Authors: Caldart RV, Fonseca EL, Freitas F, Rocha L, Vicente AC Tags: Mem Inst Oswaldo Cruz Source Type: research

Where, when, and how the diagnosis of human visceral leishmaniasis is defined: answers from the Brazilian control program.
CONCLUSIONS: VL diagnosis was predominantly conducted in hospitals with a long TD and wide application of serology. These findings may support measures focused on early diagnosis, including a greater involvement of the primary health care system. PMID: 31664313 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - November 1, 2019 Category: Infectious Diseases Authors: Luz JGG, Carvalho AG, Naves DB, Dias JVL, Fontes CJF Tags: Mem Inst Oswaldo Cruz Source Type: research

Description of a human Bocavirus recombinant strain in the Americas.
In this study three viral sequences were obtained from positives HBoV sewage samples collected in two Uruguayan cities and were characterised by different methods as recombinant strains. This recombination event was localised in the 5' end of VP1 gene and the parental strains belonged to subtypes 3 and 4. These three Uruguayan strains are identical at the nucleotide sequences in the analysed genome region of the virus. As far as we known, this study represents the first detection of HBoV recombinants strains in the Americas. PMID: 31644704 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - October 25, 2019 Category: Infectious Diseases Authors: Salvo M, Mir D, Tort LFL, Lizasoain A, Colina R, Victoria M Tags: Mem Inst Oswaldo Cruz Source Type: research

A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses.
CONCLUSIONS: These findings indicate that the patient was infected by two genetically and biologically distinct S. brasiliensis that vary in their production of melanin in their environmental forms. The results underscore the importance of characterizing phenotypically different isolates found in the same clinical specimen or patient. PMID: 31644705 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - October 25, 2019 Category: Infectious Diseases Authors: Oliveira MME, Almeida-Paes R, Corrêa-Moreira D, Borba CM, Menezes RC, Freitas DFS, do Valle ACF, Schubach AO, Barros MBL, Nosanchuk JD, Gutierrez-Galhardo MC, Zancopé-Oliveira RM Tags: Mem Inst Oswaldo Cruz Source Type: research

Human virome in nasopharynx and tracheal secretion samples.
CONCLUSIONS: Wide virus diversity was found by different methodologies and high frequency of rhinovirus occurrence was confirmed in population in winter, showing its relevance for public health. PMID: 31596309 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - October 10, 2019 Category: Infectious Diseases Authors: Souza LDC, Blawid R, Silva JMF, Nagata T Tags: Mem Inst Oswaldo Cruz Source Type: research

A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry.
Abstract As phagocytosis is the first line of defense against malaria, we developed a phagocytosis assay with Plasmodium vivax (P. vivax) merozoites that can be applied to evaluate vaccine candidates. Briefly, after leukocyte removal with loosely packed cellulose powder in a syringe, P. vivax trophozoites matured to the merozoite-rich schizont stages in the presence of the E64 protease inhibitor. The Percoll gradient-enriched schizonts were chemically disrupted to release merozoites that were submitted to merozoite opsonin-dependent phagocytosis in two phagocytic lines with human and mouse antibodies against the N...
Source: Memorias do Instituto Oswaldo Cruz - October 10, 2019 Category: Infectious Diseases Authors: Farias E, Bezerra F, Baia-da-Silva DC, Chaves YO, Cardoza TB, de Almeida MEM, Oliveira LB, Lalwani P, Orlandi PP, Lacerda MVG, Lopes SCP, Nogueira PA Tags: Mem Inst Oswaldo Cruz Source Type: research

Genetic diversity of Echinococcus vogeli in the western Brazilian Amazon.
Abstract Human polycystic echinococcosis is a parasitic infection caused by the larval stage of Echinococcus vogeli, which occurs in rural areas of Central and South America. Until now, little information on the genetic variability of E. vogeli is available. Here, 32 samples from human-excised E. vogeli cysts had a 396-bp sequence of the mitochondrial cytochrome oxidase I (COI) gene sequenced and compared to another 17 COI sequences representing nine Echinococcus species. A Bayesian COI tree revealed that all E. vogeli sequences formed a monophyletic and well-supported clade with an E. vogeli reference sequence. T...
Source: Memorias do Instituto Oswaldo Cruz - October 3, 2019 Category: Infectious Diseases Authors: Daipert-Garcia D, Pavan MG, Neves LBD, Almeida FB, Siqueira NG, Santos GBD, Dias-Correia TP, Ferreira HB, Rodrigues-Silva R Tags: Mem Inst Oswaldo Cruz Source Type: research

Lutzomyia longipalpis (Diptera: Psychodidae) Argentina-Bolivia border: new report and genetic diversity.
oacute;n OD Abstract American visceral leishmaniasis (AVL) has two main scenarios of transmission as follows: scattered cases in rural areas and urban outbreaks. Urban AVL is in active dispersion from the northeastern border of Argentina-Paraguay-Brazil to the South. The presence of Lutzomyia longipalpis was initially reported in urban environments in the northwestern border of the country. The presence of Lu. longipalpis, environmental variables associated with its distribution, and its genetic diversity were assessed in Salvador Mazza, Argentina, on the border with Bolivia. The genetic analysis showed high haplo...
Source: Memorias do Instituto Oswaldo Cruz - October 3, 2019 Category: Infectious Diseases Authors: Quintana MG, Pech-May A, Fuenzalida AD, Direni Mancini JM, Barroso PA, Yadón ZE, Zaidenberg M, Salomón OD Tags: Mem Inst Oswaldo Cruz Source Type: research

Liaisons dangereuses: cross-border gene flow and dispersal of insecticide resistance-associated genes in the mosquito Aedes aegypti from Brazil and French Guiana.
CONCLUSIONS: The present study highlights the need for regional-local vector surveillance and transnational collaboration between neighboring countries to assess the impact of implemented vector control strategies, promote timely actions and develop preparedness plans. PMID: 31553370 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - September 26, 2019 Category: Infectious Diseases Authors: Salgueiro P, Restrepo-Zabaleta J, Costa M, Galardo AKR, Pinto J, Gaborit P, Guidez A, Martins AJ, Dusfour I Tags: Mem Inst Oswaldo Cruz Source Type: research

Calpains of Leishmania braziliensis: genome analysis, differential expression, and functional analysis.
CONCLUSION: This report adds novel insights into the domain structure, expression, and localisation of L. braziliensis calpain-like molecules. PMID: 31553371 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - September 26, 2019 Category: Infectious Diseases Authors: Ennes-Vidal V, Vitório BDS, Menna-Barreto RFS, Pitaluga AN, Gonçalves-da-Silva SA, Branquinha MH, Santos ALS, d'Avila-Levy CM Tags: Mem Inst Oswaldo Cruz Source Type: research

Molecular epidemiology of Hepatitis delta virus infection in Minas Gerais state from Brazil, an area outside the hyperendemic region of the Amazon Basin.
CONCLUSIONS: This study confirmed the circulation of HDV in Minas Gerais, an area previously considered non-endemic for hepatitis D in Brazil. The prevalence found in this study is much higher when compared to other studies performed in Brazil, probably because the population in our study was selected with minimal bias. Furthermore, in 26 anti-HD positive plasma samples, we were also able to detect the viral genome, indicating that these patients were experienced an active infection at the time of sample collection. These findings emphasise the importance of anti-HD testing in HBV infected individuals, which may contribute...
Source: Memorias do Instituto Oswaldo Cruz - August 30, 2019 Category: Infectious Diseases Authors: Scarponi CF, Kroon EG, Vieira DS, Fernandes AP, Gomes KB, Mota BE Tags: Mem Inst Oswaldo Cruz Source Type: research

Genetic and biological characterisation of Zika virus isolates from different Brazilian regions.
CONCLUSIONS: Genomic analysis of three recent ZIKV isolates revealed some nonsynonymous substitutions, which could have an impact on the viral fitness in mammalian and insect cells. PMID: 31432892 [PubMed - in process] (Source: Memorias do Instituto Oswaldo Cruz)
Source: Memorias do Instituto Oswaldo Cruz - August 24, 2019 Category: Infectious Diseases Authors: Strottmann DM, Zanluca C, Mosimann ALP, Koishi AC, Auwerter NC, Faoro H, Cataneo AHD, Kuczera D, Wowk PF, Bordignon J, Duarte Dos Santos CN Tags: Mem Inst Oswaldo Cruz Source Type: research

Decentralising scientific publishing: can the blockchain improve science communication?
We present a decentralised solution for managing scientific communication, based on distributed ledger technologies, also called blockchains. The proposed system aims to solve incentive problems displayed by traditional systems in scientific communication and publication. A minimal working model is presented, defining roles, processes, and expected results from the novel system. The proposed solution is viable, given the current status of blockchain technology, and should lead to a rethinking of current practices and their consequences for scientific communication. PMID: 31432893 [PubMed - in process] (Source: Memoria...
Source: Memorias do Instituto Oswaldo Cruz - August 24, 2019 Category: Infectious Diseases Authors: Coelho FC, Brandão A Tags: Mem Inst Oswaldo Cruz Source Type: research