Characterization of mRNA therapeutics
Mass Spectrom Rev. 2023 Jul 4. doi: 10.1002/mas.21856. Online ahead of print.ABSTRACTTherapeutic messenger RNAs (mRNAs) have emerged as powerful tools in the treatment of complex diseases, especially for conditions that lack efficacious treatment. The successful application of this modality can be attributed to its ability to encode entire proteins. While the large nature of these molecules has supported their success as therapeutics, its extended size creates several analytical challenges. To further support therapeutic mRNA development and its deployment in clinical trials, appropriate methods to support their characteri...
Source: Mass Spectrometry Reviews - July 4, 2023 Category: Molecular Biology Authors: Guilherme J Guimaraes Jaeah Kim Michael G Bartlett Source Type: research
Ion-molecule studies of energetic oxygen allotropes in flow tubes: < mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" > < mml:semantics > < mml:mrow > < mml:msub > < mml:mi > O < /mml:mi > < mml:mn > 2 < /mml:mn > < /mml:msub > < mml:mo > ( < /mml:mo > < mml:mi > v < /mml:mi > < mml:mo > ) < /mml:mo > < mml:mo > , < /mml:mo > < mml:msub > < mml:mi > O < /mml:mi > < mml:mn > 2 < /mml:mn > < /mml:msub > < mml:mo > ( < /mml:mo > < mml:mi > a < /mml:mi > < mml:mmultiscripts > < mml:mi > Δ < /mml:mi > < mml:mi > g < /mml:mi > < mml:none > < /mml:none > < mml:mprescripts > < /mml:mprescripts > < mml:none > < /mml:none > < mml:mn > 1 < /mml:mn > < /mml:mmultiscripts > < mml:mo > ) < /mml:mo > < mml:mo > , < /mml:mo > < mml:msub > < mml:mi > O < /mml:mi > < mml:mn > 3 < /mml:mn > < /mml:msub > < mml:mo > , < /mml:mo > < mml:mi > and < /mml:mi > < mml:mi > O < /mml:mi > < /mml:mrow > < mml:annotation > ${{\rm{O}}}_{2}({\rm{v}}),{{\rm{O}}}_{2}({\rm{a}}{}^{1}{\rm{\Delta }}_{{\rm{g}}}),{{\rm{O}}}_{3},\mathrm{and}{\rm{O}}$ < /mml:annotation > < /mml:semantics > < /mml:math >
Mass Spectrom Rev. 2023 Jul 2. doi: 10.1002/mas.21846. Online ahead of print.ABSTRACTStarting in the 1960s, flow tube apparatuses have played a central role in the study of ion-molecule kinetics, allowing for immense chemical diversity of cationic, anionic, and neutral reactants. Here, we review studies of oxygen allotropes, excluding ground state O2 ( X 3 ∑ g - ${X}^{3}{<mpadded xmlns="http://www.w3.org/1998/Math/MathML">\sum </mpadded>}_{g}^{-}$ ), and focusing instead on reactions of cations, anions, and metal chemi-ionization reactions with ground state atomic oxygen (O 3 P), vibrationally excited mol...
Source: Mass Spectrometry Reviews - July 3, 2023 Category: Molecular Biology Authors: Nicole Eyet Shaun G Ard Nicholas S Shuman Albert A Viggiano Source Type: research
Peptides as "better biomarkers"? Value, challenges, and potential solutions to facilitate implementation
Mass Spectrom Rev. 2023 Jun 26. doi: 10.1002/mas.21854. Online ahead of print.ABSTRACTPeptides carry important functions in normal physiological and pathophysiological processes and can serve as clinically useful biomarkers. Given the ability to diffuse passively across endothelial barriers, endogenous peptides can be examined in several body fluids, including among others urine, blood, and cerebrospinal fluid. This review article provides an update on the recently published literature that reports on investigating native peptides in body fluids using mass spectrometry-based platforms, specifically those studies that focus...
Source: Mass Spectrometry Reviews - June 26, 2023 Category: Molecular Biology Authors: Agnieszka Latosinska Maria Frantzi Justyna Siwy Source Type: research
Peptides as "better biomarkers"? Value, challenges, and potential solutions to facilitate implementation
Mass Spectrom Rev. 2023 Jun 26. doi: 10.1002/mas.21854. Online ahead of print.ABSTRACTPeptides carry important functions in normal physiological and pathophysiological processes and can serve as clinically useful biomarkers. Given the ability to diffuse passively across endothelial barriers, endogenous peptides can be examined in several body fluids, including among others urine, blood, and cerebrospinal fluid. This review article provides an update on the recently published literature that reports on investigating native peptides in body fluids using mass spectrometry-based platforms, specifically those studies that focus...
Source: Mass Spectrometry Reviews - June 26, 2023 Category: Molecular Biology Authors: Agnieszka Latosinska Maria Frantzi Justyna Siwy Source Type: research
Peptides as "better biomarkers"? Value, challenges, and potential solutions to facilitate implementation
Mass Spectrom Rev. 2023 Jun 26. doi: 10.1002/mas.21854. Online ahead of print.ABSTRACTPeptides carry important functions in normal physiological and pathophysiological processes and can serve as clinically useful biomarkers. Given the ability to diffuse passively across endothelial barriers, endogenous peptides can be examined in several body fluids, including among others urine, blood, and cerebrospinal fluid. This review article provides an update on the recently published literature that reports on investigating native peptides in body fluids using mass spectrometry-based platforms, specifically those studies that focus...
Source: Mass Spectrometry Reviews - June 26, 2023 Category: Molecular Biology Authors: Agnieszka Latosinska Maria Frantzi Justyna Siwy Source Type: research
Peptides as "better biomarkers"? Value, challenges, and potential solutions to facilitate implementation
Mass Spectrom Rev. 2023 Jun 26. doi: 10.1002/mas.21854. Online ahead of print.ABSTRACTPeptides carry important functions in normal physiological and pathophysiological processes and can serve as clinically useful biomarkers. Given the ability to diffuse passively across endothelial barriers, endogenous peptides can be examined in several body fluids, including among others urine, blood, and cerebrospinal fluid. This review article provides an update on the recently published literature that reports on investigating native peptides in body fluids using mass spectrometry-based platforms, specifically those studies that focus...
Source: Mass Spectrometry Reviews - June 26, 2023 Category: Molecular Biology Authors: Agnieszka Latosinska Maria Frantzi Justyna Siwy Source Type: research
Peptides as "better biomarkers"? Value, challenges, and potential solutions to facilitate implementation
Mass Spectrom Rev. 2023 Jun 26. doi: 10.1002/mas.21854. Online ahead of print.ABSTRACTPeptides carry important functions in normal physiological and pathophysiological processes and can serve as clinically useful biomarkers. Given the ability to diffuse passively across endothelial barriers, endogenous peptides can be examined in several body fluids, including among others urine, blood, and cerebrospinal fluid. This review article provides an update on the recently published literature that reports on investigating native peptides in body fluids using mass spectrometry-based platforms, specifically those studies that focus...
Source: Mass Spectrometry Reviews - June 26, 2023 Category: Molecular Biology Authors: Agnieszka Latosinska Maria Frantzi Justyna Siwy Source Type: research
Peptides as "better biomarkers"? Value, challenges, and potential solutions to facilitate implementation
Mass Spectrom Rev. 2023 Jun 26. doi: 10.1002/mas.21854. Online ahead of print.ABSTRACTPeptides carry important functions in normal physiological and pathophysiological processes and can serve as clinically useful biomarkers. Given the ability to diffuse passively across endothelial barriers, endogenous peptides can be examined in several body fluids, including among others urine, blood, and cerebrospinal fluid. This review article provides an update on the recently published literature that reports on investigating native peptides in body fluids using mass spectrometry-based platforms, specifically those studies that focus...
Source: Mass Spectrometry Reviews - June 26, 2023 Category: Molecular Biology Authors: Agnieszka Latosinska Maria Frantzi Justyna Siwy Source Type: research
Analysis of triacylglycerol and phospholipid sn-positional isomers by liquid chromatographic and mass spectrometric methodologies
Mass Spectrom Rev. 2023 Jun 6. doi: 10.1002/mas.21853. Online ahead of print.ABSTRACTAnalysis of triacylglycerol (TG) and phospholipid sn-positional isomers can be divided into two main categories: (a) direct separation by chromatography or other means such as ion mobility mass spectrometry and (b) quantification of regioisomer ratios by structurally informative fragment ions with mass spectrometric methods. Due to long retention times and limited performance, researchers are moving away from direct chromatographic separation of isomers, using mass spectrometry instead. Many established analytical methods are targeting spe...
Source: Mass Spectrometry Reviews - June 6, 2023 Category: Molecular Biology Authors: Mikael Fabritius Baoru Yang Source Type: research
Analysis of triacylglycerol and phospholipid sn-positional isomers by liquid chromatographic and mass spectrometric methodologies
Mass Spectrom Rev. 2023 Jun 6. doi: 10.1002/mas.21853. Online ahead of print.ABSTRACTAnalysis of triacylglycerol (TG) and phospholipid sn-positional isomers can be divided into two main categories: (a) direct separation by chromatography or other means such as ion mobility mass spectrometry and (b) quantification of regioisomer ratios by structurally informative fragment ions with mass spectrometric methods. Due to long retention times and limited performance, researchers are moving away from direct chromatographic separation of isomers, using mass spectrometry instead. Many established analytical methods are targeting spe...
Source: Mass Spectrometry Reviews - June 6, 2023 Category: Molecular Biology Authors: Mikael Fabritius Baoru Yang Source Type: research
Analysis of triacylglycerol and phospholipid sn-positional isomers by liquid chromatographic and mass spectrometric methodologies
Mass Spectrom Rev. 2023 Jun 6. doi: 10.1002/mas.21853. Online ahead of print.ABSTRACTAnalysis of triacylglycerol (TG) and phospholipid sn-positional isomers can be divided into two main categories: (a) direct separation by chromatography or other means such as ion mobility mass spectrometry and (b) quantification of regioisomer ratios by structurally informative fragment ions with mass spectrometric methods. Due to long retention times and limited performance, researchers are moving away from direct chromatographic separation of isomers, using mass spectrometry instead. Many established analytical methods are targeting spe...
Source: Mass Spectrometry Reviews - June 6, 2023 Category: Molecular Biology Authors: Mikael Fabritius Baoru Yang Source Type: research
Analysis of triacylglycerol and phospholipid sn-positional isomers by liquid chromatographic and mass spectrometric methodologies
Mass Spectrom Rev. 2023 Jun 6. doi: 10.1002/mas.21853. Online ahead of print.ABSTRACTAnalysis of triacylglycerol (TG) and phospholipid sn-positional isomers can be divided into two main categories: (a) direct separation by chromatography or other means such as ion mobility mass spectrometry and (b) quantification of regioisomer ratios by structurally informative fragment ions with mass spectrometric methods. Due to long retention times and limited performance, researchers are moving away from direct chromatographic separation of isomers, using mass spectrometry instead. Many established analytical methods are targeting spe...
Source: Mass Spectrometry Reviews - June 6, 2023 Category: Molecular Biology Authors: Mikael Fabritius Baoru Yang Source Type: research
Analysis of triacylglycerol and phospholipid sn-positional isomers by liquid chromatographic and mass spectrometric methodologies
Mass Spectrom Rev. 2023 Jun 6. doi: 10.1002/mas.21853. Online ahead of print.ABSTRACTAnalysis of triacylglycerol (TG) and phospholipid sn-positional isomers can be divided into two main categories: (a) direct separation by chromatography or other means such as ion mobility mass spectrometry and (b) quantification of regioisomer ratios by structurally informative fragment ions with mass spectrometric methods. Due to long retention times and limited performance, researchers are moving away from direct chromatographic separation of isomers, using mass spectrometry instead. Many established analytical methods are targeting spe...
Source: Mass Spectrometry Reviews - June 6, 2023 Category: Molecular Biology Authors: Mikael Fabritius Baoru Yang Source Type: research
Analysis of triacylglycerol and phospholipid sn-positional isomers by liquid chromatographic and mass spectrometric methodologies
Mass Spectrom Rev. 2023 Jun 6. doi: 10.1002/mas.21853. Online ahead of print.ABSTRACTAnalysis of triacylglycerol (TG) and phospholipid sn-positional isomers can be divided into two main categories: (a) direct separation by chromatography or other means such as ion mobility mass spectrometry and (b) quantification of regioisomer ratios by structurally informative fragment ions with mass spectrometric methods. Due to long retention times and limited performance, researchers are moving away from direct chromatographic separation of isomers, using mass spectrometry instead. Many established analytical methods are targeting spe...
Source: Mass Spectrometry Reviews - June 6, 2023 Category: Molecular Biology Authors: Mikael Fabritius Baoru Yang Source Type: research
Analysis of triacylglycerol and phospholipid sn-positional isomers by liquid chromatographic and mass spectrometric methodologies
Mass Spectrom Rev. 2023 Jun 6. doi: 10.1002/mas.21853. Online ahead of print.ABSTRACTAnalysis of triacylglycerol (TG) and phospholipid sn-positional isomers can be divided into two main categories: (a) direct separation by chromatography or other means such as ion mobility mass spectrometry and (b) quantification of regioisomer ratios by structurally informative fragment ions with mass spectrometric methods. Due to long retention times and limited performance, researchers are moving away from direct chromatographic separation of isomers, using mass spectrometry instead. Many established analytical methods are targeting spe...
Source: Mass Spectrometry Reviews - June 6, 2023 Category: Molecular Biology Authors: Mikael Fabritius Baoru Yang Source Type: research
