Comparing plasma and skin imprint metabolic profiles in COVID-19 diagnosis and severity assessment
AbstractAs SARS-CoV-2 continues to produce new variants, the demand for diagnostics and a better understanding of COVID-19 remain key topics in healthcare. Skin manifestations have been widely reported in cases of COVID-19, but the mechanisms and markers of these symptoms are poorly described. In this cross-sectional study, 101 patients (64 COVID-19 positive patients and 37 controls) were enrolled between April and June 2020, during the first wave of COVID-19, in S ão Paulo, Brazil. Enrolled patients had skin imprints sampled non-invasively using silica plates; plasma samples were also collected. Samples were used for unt...
Source: Journal of Molecular Medicine - November 27, 2023 Category: Molecular Biology Source Type: research
IL-23 contributes to Particulate Matter induced allergic asthma in the early life of mice and promotes asthma susceptibility
This study aimed to investigate changes in allergic phenotypes and effects on allergen-specific memory T cells resulting from co-exposure of mice in the early life to PM and house dust mites (HDM) and to explore the role of interleukin-23 (IL-23) in this process. PM and low-dose HDM were administered intranasally in 4-day-old C57BL/6 mice. After confirming an increase in IL-23 expression in mouse lung tissues, changes in the asthma phenotype and lung effector/memory Th2 or Th17 cells were evaluated after intranasal administration of anti-IL-23 antibody (Ab) during co-exposure to PM and HDM. Evaluation was performed up to 7...
Source: Journal of Molecular Medicine - November 23, 2023 Category: Molecular Biology Source Type: research
Metabolomics reveals that chronic restraint stress alleviates carbon tetrachloride-induced hepatic fibrosis through the INSR/PI3K/AKT/AMPK pathway
AbstractHepatic fibrosis (HF) could be developed into liver cirrhosis or even hepatocellular carcinoma. Stress has an important role in the occurrence and development of various considerable diseases. However, the effect of a certain degree stress on HF is still controversial. In our study, stress was simulated with regular chronic restraint stress (CRS) and HF model was induced with CCl4 in mice. We found that CRS was able to attenuate CCl4-induced liver injury and fibrosis in mice. Surprisingly, behavioral analysis showed that the mice in the HF group exhibited depression-like behavior. Further, the metabolomic analysis ...
Source: Journal of Molecular Medicine - November 23, 2023 Category: Molecular Biology Source Type: research
The role of CD146 in renal disease: from experimental nephropathy to clinics
AbstractVascular endothelial dysfunction is a major risk factor in the development of renal diseases. Recent studies pointed out a major interest for the inter-endothelial junction protein CD146, as its expression is modulated during renal injury. Indeed, some complex mechanisms involving this adhesion molecule and its multiple ligands are observed in a large number of renal diseases in fundamental or clinical research. The purpose of this review is to summarize the most recent literature on the role of CD146 in renal pathophysiology, from experimental nephropathy to clinical trials. (Source: Journal of Molecular Medicine)
Source: Journal of Molecular Medicine - November 23, 2023 Category: Molecular Biology Source Type: research
A gene therapy targeting medium-chain acyl-CoA dehydrogenase (MCAD) did not protect against diabetes-induced cardiac pathology
AbstractDiabetic cardiomyopathy describes heart disease in patients with diabetes who have no other cardiac conditions but have a higher risk of developing heart failure. Specific therapies to treat the diabetic heart are limited. A key mechanism involved in the progression of diabetic cardiomyopathy is dysregulation of cardiac energy metabolism. The aim of this study was to determine if increasing the expression of medium-chain acyl-coenzyme A dehydrogenase (MCAD; encoded byAcadm), a key regulator of fatty acid oxidation, could improve the function of the diabetic heart. Male mice were administered streptozotocin to induc...
Source: Journal of Molecular Medicine - November 21, 2023 Category: Molecular Biology Source Type: research
CTHRC1 promotes colorectal cancer progression by recruiting tumor-associated macrophages via up-regulation of CCL15
In conclusion, CTHRC1 could promote the chemotactic ability of macrophages by up-regulating CCL15 via TGFβ/Smad pathway; additionally, a high level of CTHRC1 could promote macrop hage’s M2 polarization. This discovery may be related to tumor immune tolerance and tumor immunotherapy resistance in CRC.Key messagesCTHRC1 promotes CRC progression by up-regulating CCL15 via TGF- β/Smad pathways to further recruit tumor-associated macrophages.By the means of autocrine or paracrine, CTHRC1 can indeed promote macrophage chemotaxis and enhance the infiltration of macrophages in tumor tissues but in the presence of tumor cells.C...
Source: Journal of Molecular Medicine - November 21, 2023 Category: Molecular Biology Source Type: research
Progress in diagnosis and treatment of Leber ’s hereditary optic neuropathy
This article summarizes the research progress on molecular pathogenesis, clinical symptoms, and treatment of LHON in recent years, aiming to summarize the genetic pathogenesis and clinical treatment points of LHON. (Source: Journal of Molecular Medicine)
Source: Journal of Molecular Medicine - November 20, 2023 Category: Molecular Biology Source Type: research
Identifying survival of pan-cancer patients under immunotherapy using genomic mutation signature with large sample cohorts
AbstractAlthough immune checkpoint inhibitors have led to durable clinical response in multiple cancers, only a small proportion of patients respond to this treatment. Therefore, we aim to develop a predictive model that utilizes gene mutation profiles to accurately identify the survival of pan-cancer patients with immunotherapy. Here, we develop and evaluate three different nomograms using two cohorts containing 1,594 cancer patients whose mutation profiles are obtained by MSK-IMPACT sequencing and 230 cancer patients receiving whole-exome sequencing, respectively. Using eighteen genes (SETD2, BRAF, NCOA3, LATS1, IL7R, CR...
Source: Journal of Molecular Medicine - November 18, 2023 Category: Molecular Biology Source Type: research
Correction to: Downregulation of microRNA-193a-3p is involved in invertebral disc degeneration by targeting MMP14
(Source: Journal of Molecular Medicine)
Source: Journal of Molecular Medicine - November 16, 2023 Category: Molecular Biology Source Type: research
Correction to: Myeloid ‑specific deletion of chitinase‑3‑like 1 protein ameliorates murine diet‑induced steatohepatitis progression
(Source: Journal of Molecular Medicine)
Source: Journal of Molecular Medicine - November 12, 2023 Category: Molecular Biology Source Type: research
Two-trace two-dimensional correlation spectra (2T2D-COS) analysis using FTIR spectra to monitor the immune response by COVID-19
AbstractThere is a growing trend in using saliva for SARS-CoV-2 detection with reasonable accuracy. We have studied the responses of IgA, IgG, and IgM in human saliva by directly comparing disease with control analyzing two-trace two-dimensional correlation spectra (2T2D-COS) employing Fourier transform infrared (FTIR) spectra. It explores the molecular-level variation between control and COVID-19 saliva samples. The advantage of 2T2D spectra is that it helps in discriminating remarkably subtle features between two simple pairs of spectra. It gives spectral information from highly overlapped bands associated with different...
Source: Journal of Molecular Medicine - November 10, 2023 Category: Molecular Biology Source Type: research
Correction to: Microdevices for cancer stem cell culture as a predictive chemotherapeutic response platform
(Source: Journal of Molecular Medicine)
Source: Journal of Molecular Medicine - November 6, 2023 Category: Molecular Biology Source Type: research
Autophagy and nuclear morphometry are associated with histopathologic features in esophageal squamous cell carcinoma
This study evaluated the differential levels of three key regulators of autophagy (SQSTM1, MAP1LC3B, and BECN1) in patients with ESCC, associating autophagy with histopathologic features, including the grade of differentiation, mitotic rate, inflammation score, and the intensity of tumor-infiltrating lymphocytes. Nuclear morphometry of the tumor parenchyma was also assessed, associating it with autophagy and histopathology. All three markers significantly increased in patients with ESCC compared to the control group. Based on the mean expression of each protein in the control group, 57% of patients with ESCC had high level...
Source: Journal of Molecular Medicine - October 25, 2023 Category: Molecular Biology Source Type: research
Circulating extracellular vesicles are associated with pathophysiological condition including metabolic syndrome-related dysmetabolism in children and adolescents with obesity
AbstractObesity of children and adolescents (OCA) is often accompanied by metabolic syndrome (MetS), which often leads to adult obesity and subsequent complications, yet the entire pathophysiological response is not fully understood. The number and composition of circulating extracellular vesicles (EV) reflect overall patient condition; therefore, we investigated the pathophysiological condition of OCA, including MetS-associated dysmetabolism, using circulating EVs. In total, 107 children and adolescents with or without obesity (boys,n = 69; girls,n = 38; median age, 10 years) were enrolled. Circulating EV number ...
Source: Journal of Molecular Medicine - October 24, 2023 Category: Molecular Biology Source Type: research
Soluble LDL-receptor is induced by TNF- α and inhibits hepatocytic clearance of LDL-cholesterol
AbstractDefective LDL-C clearance and hence its elevation in the circulation is an established risk factor for cardiovascular diseases (CVDs) such as myocardial infarction (MI). A soluble LDL-receptor (sLDL-R) has been detected in human plasma which correlates strongly with circulating LDL-C and classical conditions that promote chronic inflammation. However, the mechanistic interplay between sLDL-R, inflammation, and CVDs remains to be investigated. Here, we report that stimulation of HepG2 cells with TNF-α induces the release of sLDL-R into culture supernatants. In addition, TNF-α induces gene expression of peptidase...
Source: Journal of Molecular Medicine - October 20, 2023 Category: Molecular Biology Source Type: research
