Retraction Note: Antioxidative effects of polypyrimidine tract-binding protein-associated splicing factor against pathological retinal angiogenesis through promotion of mitochondrial function
(Source: Journal of Molecular Medicine)
Source: Journal of Molecular Medicine - August 10, 2023 Category: Molecular Biology Source Type: research
Suppression of PGE2/EP2 signaling alleviates Hirschsprung disease by upregulating p38 mitogen-activated protein kinase activity
In this study, anEdnrb-deficient mouse model of HSCR was generated and used to investigate if PGE2/EP2 pathway could be a potential therapeutic target for HSCR. We found that downregulation of PGE2/EP2 signaling by siRNA-mediated ablation of a PGE2 synthase or pharmacologic blockage of EP2 enhanced ENCC colonization in the distal bowel ofEdnrb−/− mice and alleviated their HSCR-like symptoms. Furthermore, blockage of EP2 was shown to promote ENCC migration through upregulating p38 mitogen-activated protein kinase activity, which was downregulated in the colon ofEdnrb−/− mice and in the distal aganglionic bowel of HS...
Source: Journal of Molecular Medicine - July 31, 2023 Category: Molecular Biology Source Type: research
Molecular therapy of cardiac ischemia –reperfusion injury based on mitochondria and ferroptosis
AbstractExcessive death of myocardial cells can lead to various cardiovascular diseases and even develop into heart failure, so developing ideal treatment plans based on pathogenesis is of great significance for cardiopathy. After the heart undergoes ischemia ‒reperfusion (I/R), myocardial cells accumulate a large amount of peroxides, leading to mitochondrial dysfunction and inducing ferroptosis. Ferroptosis is a form of iron-dependent regulatory cell death (RCD) caused by imbalanced redox and iron metabolism that leads to severe cell damage through th e accumulation of peroxides. The mechanism of ferroptosis is highly c...
Source: Journal of Molecular Medicine - July 28, 2023 Category: Molecular Biology Source Type: research
CREB-H is a stress-regulator of hepcidin gene expression during early postnatal development
In conclusion, CREB-H has a role in maintaining the homeostatic balance of iron traffic through hepcidin during the critical postnatal period and in response to iron challenge.Key messagesCREB-H KO mice develop liver iron overload shortly after weaning that normalizes in adulthood.CHEB-H is involved in hepcidin gene response to oral iron in vivo.CREB-H loss hampers hepcidin promoter response to BMP6.CREB-H is a key stress-sensor controlling hepcidin gene transcription in physiologic and pathophysiologic states. (Source: Journal of Molecular Medicine)
Source: Journal of Molecular Medicine - July 26, 2023 Category: Molecular Biology Source Type: research
Transcriptional repression of lncRNA and miRNA subsets mediated by LRF during erythropoiesis
AbstractNon-coding RNA (ncRNA) species, mainly long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) have been currently imputed for lesser or greater involvement in human erythropoiesis. These RNA subsets operate within a complex circuit with other epigenetic components and transcription factors (TF) affecting chromatin remodeling during cell differentiation. Lymphoma/leukemia-related (LRF) TF exerts higher occupancy on DNA CpG rich sites and is implicated in several differentiation cell pathways and erythropoiesis among them and also directs the epigenetic regulation of hemoglobin transversion from fetal (HbF) to adult (...
Source: Journal of Molecular Medicine - July 24, 2023 Category: Molecular Biology Source Type: research
Eucalyptol induces osteoblast differentiation through ERK phosphorylation in vitro and in vivo
AbstractEucalyptol (EU) is monoterpene oxide that is the main component of the essential oil extracted from aromatic plants such asEucalyptus globules. EU has therapeutic effects such as antibacterial, anti-inflammatory and antioxidant in chronic diseases including inflammation disorder, respiratory disease, and diabetic disease. However, the effects of EU on osteoblast differentiation and bone diseases such as osteoporosis have not been studied. The present study investigated the effects of EU on osteoblast differentiation and bone formation. EU induces mRNA and protein expression of osteogenic genes in osteoblast cell li...
Source: Journal of Molecular Medicine - July 20, 2023 Category: Molecular Biology Source Type: research
Genotypic analysis of a large cohort of patients with suspected atypical hemolytic uremic syndrome
AbstractAtypical hemolytic uremic syndrome (aHUS) is characterized by microangiopathic hemolytic anemia, thrombocytopenia, and renal impairment. Complement and coagulation gene variants have been associated with aHUS susceptibility. We assessed the diagnostic yield of a next-generation sequencing (NGS) panel in a large cohort of Canadian patients with suspected aHUS. Molecular testing was performed on peripheral blood DNA samples from 167 patients, collected between May 2019 and December 2021, using a clinically validated NGS pipeline. Coding exons with 20 base pairs of flanking intronic regions for 21 aHUS-associated or c...
Source: Journal of Molecular Medicine - July 19, 2023 Category: Molecular Biology Source Type: research
The artificial oxygen carrier erythrocruorin —characteristics and potential significance in medicine
AbstractThe diminishing supply and increasing costs of donated blood have motivated research into novel hemoglobin-based oxygen carriers (HBOCs) that can serve as red blood cell (RBC) substitutes. HBOCs are versatile agents that can be used in the treatment of hemorrhagic shock. However, many of the RBC substitutes that are based on mammalian hemoglobins have presented key limitations such as instability and toxicity. In contrast, erythrocruorins (Ecs) are other types of HBOCs that may not suffer these disadvantages. Ecs are giant metalloproteins found in annelids, crustaceans, and some other invertebrates. Thus far, the E...
Source: Journal of Molecular Medicine - July 18, 2023 Category: Molecular Biology Source Type: research
Novel insights into the nervous system affected by prolonged hyperglycemia
AbstractMultiple molecular pathways including the receptor for advanced glycation end-products-diaphanous related formin 1 (RAGE-Diaph1) signaling are known to play a role in diabetic peripheral neuropathy (DPN). Evidence suggests that neuropathological alterations in type 1 diabetic spinal cord may occur at the same time as or following peripheral nerve abnormalities. We demonstrated that DPN was associated with perturbations of RAGE-Diaph1 signaling pathway in peripheral nerve accompanied by widespread spinal cord molecular changes. More than 500 differentially expressed genes (DEGs) belonging to multiple functional path...
Source: Journal of Molecular Medicine - July 18, 2023 Category: Molecular Biology Source Type: research
Auricular fistula: a review of its clinical manifestations, genetics, and treatments
This article provides a comprehensive review of auricular fistula, especially congenital preauricular fistula, which accounts for the majority of auricular fistula, by summarizing the clinical manifestations, histological and embryological development, genetics, examinations, and treatments, as well as syndromes with auricular fistula. (Source: Journal of Molecular Medicine)
Source: Journal of Molecular Medicine - July 17, 2023 Category: Molecular Biology Source Type: research
Clinical significance of mutational variants in beta and alpha genes in patients with hemoglobinopathies from two large Greek centers: a complex interplay between genotype and phenotype
AbstractHemoglobinopathies affect patients in the wider Mediterranean area consisting of 4 distinct subgroups: beta thalassemia major (TM), beta thalassemia intermedia (TI), sickle cell disease (SCD) and hemoglobin H disease (alpha thalassemia). The clinical spectrum varies from mild to severe. Complex interactions between genes and environmental factors form the clinical manifestations. There is an unmet need to clarify these multifactorial mechanisms. This is the first Greek study describing mutational alleles (HBB and HBA1/HBA2 gene variants) in 217 patients with hemoglobinopathies of two large centers in Greece (Lari...
Source: Journal of Molecular Medicine - July 7, 2023 Category: Molecular Biology Source Type: research
Interferons as negative regulators of ILC2s in allergic lung inflammation and respiratory viral infections
AbstractGroup 2 innate lymphoid cells (ILC2s), characterized by a lack of antigen receptors, have been regarded as an important component of type 2 pulmonary immunity. Analogous to Th2 cells, ILC2s are capable of releasing type 2 cytokines and amphiregulin, thus playing an essential role in a variety of diseases, such as allergic diseases and virus-induced respiratory diseases. Interferons (IFNs), an important family of cytokines with potent antiviral effects, can be triggered by microbial products, microbial exposure, and pathogen infections. Interestingly, the past few years have witnessed encouraging progress in reveali...
Source: Journal of Molecular Medicine - July 6, 2023 Category: Molecular Biology Source Type: research
Nischarin expression may have differing roles in male and female melanoma patients
AbstractDue to the development of resistance to previously effective therapies, there is a constant need for novel treatment modalities for metastatic melanoma. Nischarin (NISCH) is a druggable scaffolding protein reported as a tumor suppressor and a positive prognostic marker in breast and ovarian cancers through regulation of cancer cell survival, motility and invasion. The aim of this study was to examine the expression and potential role of nischarin in melanoma. We found that nischarin expression was decreased in melanoma tissues compared to the uninvolved skin, and this was attributed to the presence of microdeletion...
Source: Journal of Molecular Medicine - June 29, 2023 Category: Molecular Biology Source Type: research
Esophageal organoids: applications and future prospects
AbstractOrganoids have been developed in the last decade as a new research tool to simulate organ cell biology and disease. Compared to traditional 2D cell lines and animal models, experimental data based on esophageal organoids are more reliable. In recent years, esophageal organoids derived from multiple cell sources have been established, and relatively mature culture protocols have been developed. Esophageal inflammation and cancer are two directions of esophageal organoid modeling, and organoid models of esophageal adenocarcinoma, esophageal squamous cell carcinoma, and eosinophilic esophagitis have been established. ...
Source: Journal of Molecular Medicine - June 29, 2023 Category: Molecular Biology Source Type: research
PARP2 promotes inflammation in psoriasis by modulating estradiol biosynthesis in keratinocytes
In this study, we assessed the role of PARP2 in a Th17-mediated inflammatory skin condition, psoriasis. We found that PARP2 mRNA expression is increased in human psoriatic lesions. Therefore, we studied the functional consequence of decreased PARP2 expression in murine and cellular human models of psoriasis. We observed that the deletion of PARP2 attenuated the imiquimod-induced psoriasis-like dermatitis in mice. Silencing of PARP2 in human keratinocytes prevented their hyperproliferation, maintained their terminal differentiation, and reduced their production of inflammatory mediators after treatment with psoriasis-mimick...
Source: Journal of Molecular Medicine - June 23, 2023 Category: Molecular Biology Source Type: research
