Diminished miR-374c-5p negatively regulates IL (interleukin)-6 in unexplained recurrent spontaneous abortion
In this study, the miRNA expression profile in peripheral blood mononuclear cells (PBMCs) from URSA patients and normal pregnant (NP) women was assessed by miRNA mi croarray and real-time quantitative reverse-transcription polymerase chain reaction (qPCR). MiRNA target prediction tools and luciferase reporter assay were used to detect direct binding between miRNAs andIL6. Functional study of administering anti-IL-6 neutralizing antibody and miR-374c-5p mimics to an URSA animal model was performed to evaluate embryo resorption rates. In the results, compared with NP women, the expression of IL-6 increased markedly in PBMCs ...
Source: Journal of Molecular Medicine - June 10, 2022 Category: Molecular Biology Source Type: research
The FOXO family of transcription factors: key molecular players in gastric cancer
AbstractGastric cancer (GC) is the fifth most frequently diagnosed cancer worldwide and the third leading cause of cancer-related death with an oncological origin. Despite its decline in incidence and mortality in recent years, GC remains a global public problem that seriously threatens patients ’ health and lives. The forkhead box O proteins (FOXOs) are a family of evolutionarily conserved transcription factors (TFs) with crucial roles in cell fate decisions. In mammals, the FOXO family consists of four members FOXO1, 3a, 4, and 6. FOXOs play crucial roles in a variety of biological proc esses, such as development, meta...
Source: Journal of Molecular Medicine - June 10, 2022 Category: Molecular Biology Source Type: research
NF- κB in control of regulatory T cell development, identity, and function
AbstractRegulatory T cells (Treg cells) act as a major rheostat regulating the strength of immune responses, enabling tolerance of harmless foreign antigens, and preventing the development of pathogenic immune responses in various disease settings such as cancer and autoimmunity. Treg cells are present in all lymphoid and non-lymphoid tissues, and the latter often fulfill important tasks required for the physiology of their host organ. The activation of NF- κB transcription factors is a central pathway for the reprogramming of gene expression in response to inflammatory but also homeostatic cues. Genetic mouse models have...
Source: Journal of Molecular Medicine - June 8, 2022 Category: Molecular Biology Source Type: research
ZMIZ proteins: partners in transcriptional regulation and risk factors for human disease
AbstractCoregulator proteins interact with signal-dependent transcription factors to modify their transcriptional activity. ZMIZ1 and ZMIZ2 (zinc finger MIZ-type containing 1 and 2) are coregulators with nonredundant functions that share unique structural characteristics. Among other interacting domains, they possess a MIZ (Msx-interacting zinc finger) that relates them to members of the protein inhibitor of activated STAT (PIAS) family and provides them the capacity to function as SUMO E3 ligases. The ZMIZ proteins stimulate the activity of various signaling pathways, including the androgen receptor (AR), P53, SMAD3/4, WN...
Source: Journal of Molecular Medicine - June 7, 2022 Category: Molecular Biology Source Type: research
Somatic non-cancerous overgrowth syndrome of obscure molecular etiology: what are the causes and options?
(Source: Journal of Molecular Medicine)
Source: Journal of Molecular Medicine - June 3, 2022 Category: Molecular Biology Source Type: research
Correction to: The protean world of non-coding RNAs in glioblastoma
(Source: Journal of Molecular Medicine)
Source: Journal of Molecular Medicine - June 1, 2022 Category: Molecular Biology Source Type: research
Leukocyte cytokine responses in adult patients with mitochondrial DNA defects
AbstractPatients with oxidative phosphorylation (OxPhos) defects causing mitochondrial diseases appear particularly vulnerable to infections. Although OxPhos defects modulate cytokine production in vitro and in animal models, little is known about how circulating leukocytes of patients with inherited mitochondrial DNA (mtDNA) defects respond to acute immune challenges. In a small cohort of healthy controls (n = 21) and patients (n = 12) with either the m.3243A > G mutation or single, large-scale mtDNA deletions, we examined (i) cytokine responses (IL-6, TNF-α, IL-1β) in response to acute lipopolysaccharid...
Source: Journal of Molecular Medicine - May 30, 2022 Category: Molecular Biology Source Type: research
Correction to: Matrix metalloproteinase ‑7 induces E‑cadherin cleavage in acid‑exposed primary human pharyngeal epithelial cells via the ROS/ERK/c‑Jun pathway
(Source: Journal of Molecular Medicine)
Source: Journal of Molecular Medicine - May 26, 2022 Category: Molecular Biology Source Type: research
Adenoviral vectors for cardiovascular gene therapy applications: a clinical and industry perspective
AbstractDespite the development of novel pharmacological treatments, cardiovascular disease morbidity and mortality remain high indicating an unmet clinical need. Viral gene therapy enables targeted delivery of therapeutic transgenes and represents an attractive platform for tackling acquired and inherited cardiovascular diseases in the future. Current cardiovascular gene therapy trials in humans mainly focus on improving cardiac angiogenesis and function. Encouragingly, local delivery of therapeutic transgenes utilising first-generation human adenovirus serotype (HAd)-5 is safe in the short term and has shown some efficac...
Source: Journal of Molecular Medicine - May 24, 2022 Category: Molecular Biology Source Type: research
Regulation of wound healing and fibrosis by galectins
AbstractGalectins are a family of proteins with at least one carbohydrate-recognition domain. Galectins are present in various tissues and organs and participate in different physiological and pathological molecular reactions in vivo. Wound healing is the basic process of traumatic disease recovery. Wound healing involves three overlapping stages: inflammation, proliferation, and remodelling. Furthermore, a comparison of wound healing with the tumour microenvironment revealed that galectin plays a key role in the wound healing process. The current review describes the role of galectin in inflammation, angiogenesis, re-epit...
Source: Journal of Molecular Medicine - May 19, 2022 Category: Molecular Biology Source Type: research
Valproic acid modulates collagen architecture in the postoperative conjunctival scar
In this study, we investigated the capacity of VPA to modulate the postoperative collagen architecture. Histochemical examination revealed that VPA treatment was associated with the formation of thinner collagen fibers in the postoperative days 7 and 14 scars. At the micrometer scale, measurements by quantitative multiphoton microscopy indicated that VPA reduced mean collagen fiber thickness by 1.25-fold. At the nanometer scale, collagen fibril thickness and diameter measured by transmission electron microscopy were decreased by 1.08- and 1.20-fold, respectively. Moreover, delicate filamentous structures in random aggregat...
Source: Journal of Molecular Medicine - May 18, 2022 Category: Molecular Biology Source Type: research
X-linked genes exhibit miR6891-5p-regulated skewing in Sj ögren’s syndrome
AbstractMany autoimmune diseases exhibit a strikingly increased prevalence in females, with primary Sj ögren’s syndrome (pSS) being the most female-predominant example. However, the molecular basis underlying the female-bias in pSS remains elusive. To address this knowledge gap, we performed genome-wide, allele-specific profiling of minor salivary gland-derived mesenchymal stromal cells (MSCs) fro m pSS patients and control subjects, and detected major differences in the regulation of X-linked genes. In control female MSCs, X-linked genes were expressed from both paternal and maternal X chromosomes with a median paterna...
Source: Journal of Molecular Medicine - May 10, 2022 Category: Molecular Biology Source Type: research
Aquaporin-4 prevents exaggerated astrocytosis and structural damage in retinal inflammation
AbstractAquaporin-4 (AQP4) is the molecular target of the immune response in neuromyelitis optica (NMO) that leads to severe structural damage in the central nervous system (CNS) and in the retina. Conversely, AQP4 might be upregulated in astrocytes as a compensatory event in multiple sclerosis. Thus, the functional relevance of AQP4 in neuroinflammation needs to be defined. Here, we tested the role of AQP4 in the retina in MOG(35 –55)-induced experimental autoimmune encephalomyelitis (EAE) using optical coherence tomography (OCT), OCT angiography, immunohistology, flow cytometry, and gene expression analysis in wild-typ...
Source: Journal of Molecular Medicine - May 10, 2022 Category: Molecular Biology Source Type: research
Correction to: ASK1 is a novel molecular target for preventing aminoglycoside ‑induced hair cell death
(Source: Journal of Molecular Medicine)
Source: Journal of Molecular Medicine - May 10, 2022 Category: Molecular Biology Source Type: research
PTEN-induced kinase 1 deficiency alters albumin permeability and insulin signaling in podocytes
In conclusion, we found that PINK1 plays an essential role in insulin signaling and the maintenance of proper permeability in podocytes. Therefore, PINK1 may be a potential therapeutic target for the treatment or prevention of diabetic nephropathy. (Source: Journal of Molecular Medicine)
Source: Journal of Molecular Medicine - May 9, 2022 Category: Molecular Biology Source Type: research
