The N-terminal disordered region of ChsB regulates its efficient transport to the hyphal apical surface in Aspergillus nidulans
In this study, we showed that ChsB is phosphorylated in vivo. We characterized strains that produce ChsB using stepwise truncations of its N-terminal disordered region or deletions of some residues in that region and demonstrated its involvement in ChsB abundance on the hyphal apical surface and in hyphal tip localization. Furthermore, we showed that some deletions in this region affected the phosphorylation states of ChsB, raising the possibility that these states are important for the localization of ChsB to the hyphal surface and the growth of A. nidulans. Our findings indicate that ChsB transport is regulated by its N-...
Source: Current Genetics - April 18, 2023 Category: Genetics & Stem Cells Authors: Jingyun Jin Ryo Iwama Hiroyuki Horiuchi Source Type: research
The N-terminal disordered region of ChsB regulates its efficient transport to the hyphal apical surface in Aspergillus nidulans
In this study, we showed that ChsB is phosphorylated in vivo. We characterized strains that produce ChsB using stepwise truncations of its N-terminal disordered region or deletions of some residues in that region and demonstrated its involvement in ChsB abundance on the hyphal apical surface and in hyphal tip localization. Furthermore, we showed that some deletions in this region affected the phosphorylation states of ChsB, raising the possibility that these states are important for the localization of ChsB to the hyphal surface and the growth of A. nidulans. Our findings indicate that ChsB transport is regulated by its N-...
Source: Current Genetics - April 18, 2023 Category: Genetics & Stem Cells Authors: Jingyun Jin Ryo Iwama Hiroyuki Horiuchi Source Type: research
The N-terminal disordered region of ChsB regulates its efficient transport to the hyphal apical surface in Aspergillus nidulans
In this study, we showed that ChsB is phosphorylated in vivo. We characterized strains that produce ChsB using stepwise truncations of its N-terminal disordered region or deletions of some residues in that region and demonstrated its involvement in ChsB abundance on the hyphal apical surface and in hyphal tip localization. Furthermore, we showed that some deletions in this region affected the phosphorylation states of ChsB, raising the possibility that these states are important for the localization of ChsB to the hyphal surface and the growth of A. nidulans. Our findings indicate that ChsB transport is regulated by its N-...
Source: Current Genetics - April 18, 2023 Category: Genetics & Stem Cells Authors: Jingyun Jin Ryo Iwama Hiroyuki Horiuchi Source Type: research
The N-terminal disordered region of ChsB regulates its efficient transport to the hyphal apical surface in Aspergillus nidulans
In this study, we showed that ChsB is phosphorylated in vivo. We characterized strains that produce ChsB using stepwise truncations of its N-terminal disordered region or deletions of some residues in that region and demonstrated its involvement in ChsB abundance on the hyphal apical surface and in hyphal tip localization. Furthermore, we showed that some deletions in this region affected the phosphorylation states of ChsB, raising the possibility that these states are important for the localization of ChsB to the hyphal surface and the growth of A. nidulans. Our findings indicate that ChsB transport is regulated by its N-...
Source: Current Genetics - April 18, 2023 Category: Genetics & Stem Cells Authors: Jingyun Jin Ryo Iwama Hiroyuki Horiuchi Source Type: research
The N-terminal disordered region of ChsB regulates its efficient transport to the hyphal apical surface in Aspergillus nidulans
In this study, we showed that ChsB is phosphorylated in vivo. We characterized strains that produce ChsB using stepwise truncations of its N-terminal disordered region or deletions of some residues in that region and demonstrated its involvement in ChsB abundance on the hyphal apical surface and in hyphal tip localization. Furthermore, we showed that some deletions in this region affected the phosphorylation states of ChsB, raising the possibility that these states are important for the localization of ChsB to the hyphal surface and the growth of A. nidulans. Our findings indicate that ChsB transport is regulated by its N-...
Source: Current Genetics - April 18, 2023 Category: Genetics & Stem Cells Authors: Jingyun Jin Ryo Iwama Hiroyuki Horiuchi Source Type: research
Tfs1, transcription elongation factor TFIIS, has an impact on chromosome segregation affected by pka1 deletion in Schizosaccharomyces pombe
Curr Genet. 2023 Apr 13. doi: 10.1007/s00294-023-01268-0. Online ahead of print.ABSTRACTThe cAMP-dependent protein kinase (PKA) pathway in Schizosaccharomyces pombe plays an important role in microtubule organization and chromosome segregation. Typically, loss of functional Pka1 induces sensitivity to the microtubule-destabilizing drug thiabendazole (TBZ) and chromosome mis-segregation. To determine the mechanism via which Pka1 is involved in these events, we explored the relevance of transcription factors by creating a double-deletion strain of pka1 and 102 individual genes encoding transcription factors. We found that rs...
Source: Current Genetics - April 13, 2023 Category: Genetics & Stem Cells Authors: Kouhei Takenaka Shiho Nishioka Yuki Nishida Makoto Kawamukai Yasuhiro Matsuo Source Type: research
Tfs1, transcription elongation factor TFIIS, has an impact on chromosome segregation affected by pka1 deletion in Schizosaccharomyces pombe
Curr Genet. 2023 Apr 13. doi: 10.1007/s00294-023-01268-0. Online ahead of print.ABSTRACTThe cAMP-dependent protein kinase (PKA) pathway in Schizosaccharomyces pombe plays an important role in microtubule organization and chromosome segregation. Typically, loss of functional Pka1 induces sensitivity to the microtubule-destabilizing drug thiabendazole (TBZ) and chromosome mis-segregation. To determine the mechanism via which Pka1 is involved in these events, we explored the relevance of transcription factors by creating a double-deletion strain of pka1 and 102 individual genes encoding transcription factors. We found that rs...
Source: Current Genetics - April 13, 2023 Category: Genetics & Stem Cells Authors: Kouhei Takenaka Shiho Nishioka Yuki Nishida Makoto Kawamukai Yasuhiro Matsuo Source Type: research
Tfs1, transcription elongation factor TFIIS, has an impact on chromosome segregation affected by pka1 deletion in Schizosaccharomyces pombe
Curr Genet. 2023 Apr 13. doi: 10.1007/s00294-023-01268-0. Online ahead of print.ABSTRACTThe cAMP-dependent protein kinase (PKA) pathway in Schizosaccharomyces pombe plays an important role in microtubule organization and chromosome segregation. Typically, loss of functional Pka1 induces sensitivity to the microtubule-destabilizing drug thiabendazole (TBZ) and chromosome mis-segregation. To determine the mechanism via which Pka1 is involved in these events, we explored the relevance of transcription factors by creating a double-deletion strain of pka1 and 102 individual genes encoding transcription factors. We found that rs...
Source: Current Genetics - April 13, 2023 Category: Genetics & Stem Cells Authors: Kouhei Takenaka Shiho Nishioka Yuki Nishida Makoto Kawamukai Yasuhiro Matsuo Source Type: research
Tfs1, transcription elongation factor TFIIS, has an impact on chromosome segregation affected by pka1 deletion in Schizosaccharomyces pombe
Curr Genet. 2023 Apr 13. doi: 10.1007/s00294-023-01268-0. Online ahead of print.ABSTRACTThe cAMP-dependent protein kinase (PKA) pathway in Schizosaccharomyces pombe plays an important role in microtubule organization and chromosome segregation. Typically, loss of functional Pka1 induces sensitivity to the microtubule-destabilizing drug thiabendazole (TBZ) and chromosome mis-segregation. To determine the mechanism via which Pka1 is involved in these events, we explored the relevance of transcription factors by creating a double-deletion strain of pka1 and 102 individual genes encoding transcription factors. We found that rs...
Source: Current Genetics - April 13, 2023 Category: Genetics & Stem Cells Authors: Kouhei Takenaka Shiho Nishioka Yuki Nishida Makoto Kawamukai Yasuhiro Matsuo Source Type: research
Tfs1, transcription elongation factor TFIIS, has an impact on chromosome segregation affected by pka1 deletion in Schizosaccharomyces pombe
Curr Genet. 2023 Apr 13. doi: 10.1007/s00294-023-01268-0. Online ahead of print.ABSTRACTThe cAMP-dependent protein kinase (PKA) pathway in Schizosaccharomyces pombe plays an important role in microtubule organization and chromosome segregation. Typically, loss of functional Pka1 induces sensitivity to the microtubule-destabilizing drug thiabendazole (TBZ) and chromosome mis-segregation. To determine the mechanism via which Pka1 is involved in these events, we explored the relevance of transcription factors by creating a double-deletion strain of pka1 and 102 individual genes encoding transcription factors. We found that rs...
Source: Current Genetics - April 13, 2023 Category: Genetics & Stem Cells Authors: Kouhei Takenaka Shiho Nishioka Yuki Nishida Makoto Kawamukai Yasuhiro Matsuo Source Type: research
Application of nanotags and nanobodies for live cell single-molecule imaging of the Z-ring in Escherichia coli
Curr Genet. 2023 Apr 6. doi: 10.1007/s00294-023-01266-2. Online ahead of print.ABSTRACTUnderstanding where proteins are localized in a bacterial cell is essential for understanding their function and regulation. This is particularly important for proteins that are involved in cell division, which localize at the division septum and assemble into highly regulated complexes. Current knowledge of these complexes has been greatly facilitated by super-resolution imaging using fluorescent protein fusions. Herein, we demonstrate with FtsZ that single-molecule PALM images can be obtained in-vivo using a genetically fused nanotag (...
Source: Current Genetics - April 6, 2023 Category: Genetics & Stem Cells Authors: Emma Westlund Axel Bergenstr åle Alaska Pokhrel Helena Chan Ulf Skoglund Daniel O Daley Bill S öderström Source Type: research
Application of nanotags and nanobodies for live cell single-molecule imaging of the Z-ring in Escherichia coli
Curr Genet. 2023 Apr 6. doi: 10.1007/s00294-023-01266-2. Online ahead of print.ABSTRACTUnderstanding where proteins are localized in a bacterial cell is essential for understanding their function and regulation. This is particularly important for proteins that are involved in cell division, which localize at the division septum and assemble into highly regulated complexes. Current knowledge of these complexes has been greatly facilitated by super-resolution imaging using fluorescent protein fusions. Herein, we demonstrate with FtsZ that single-molecule PALM images can be obtained in-vivo using a genetically fused nanotag (...
Source: Current Genetics - April 6, 2023 Category: Genetics & Stem Cells Authors: Emma Westlund Axel Bergenstr åle Alaska Pokhrel Helena Chan Ulf Skoglund Daniel O Daley Bill S öderström Source Type: research
Application of nanotags and nanobodies for live cell single-molecule imaging of the Z-ring in Escherichia coli
Curr Genet. 2023 Apr 6. doi: 10.1007/s00294-023-01266-2. Online ahead of print.ABSTRACTUnderstanding where proteins are localized in a bacterial cell is essential for understanding their function and regulation. This is particularly important for proteins that are involved in cell division, which localize at the division septum and assemble into highly regulated complexes. Current knowledge of these complexes has been greatly facilitated by super-resolution imaging using fluorescent protein fusions. Herein, we demonstrate with FtsZ that single-molecule PALM images can be obtained in-vivo using a genetically fused nanotag (...
Source: Current Genetics - April 6, 2023 Category: Genetics & Stem Cells Authors: Emma Westlund Axel Bergenstr åle Alaska Pokhrel Helena Chan Ulf Skoglund Daniel O Daley Bill S öderström Source Type: research
Application of nanotags and nanobodies for live cell single-molecule imaging of the Z-ring in Escherichia coli
Curr Genet. 2023 Apr 6. doi: 10.1007/s00294-023-01266-2. Online ahead of print.ABSTRACTUnderstanding where proteins are localized in a bacterial cell is essential for understanding their function and regulation. This is particularly important for proteins that are involved in cell division, which localize at the division septum and assemble into highly regulated complexes. Current knowledge of these complexes has been greatly facilitated by super-resolution imaging using fluorescent protein fusions. Herein, we demonstrate with FtsZ that single-molecule PALM images can be obtained in-vivo using a genetically fused nanotag (...
Source: Current Genetics - April 6, 2023 Category: Genetics & Stem Cells Authors: Emma Westlund Axel Bergenstr åle Alaska Pokhrel Helena Chan Ulf Skoglund Daniel O Daley Bill S öderström Source Type: research
Application of nanotags and nanobodies for live cell single-molecule imaging of the Z-ring in Escherichia coli
Curr Genet. 2023 Apr 6. doi: 10.1007/s00294-023-01266-2. Online ahead of print.ABSTRACTUnderstanding where proteins are localized in a bacterial cell is essential for understanding their function and regulation. This is particularly important for proteins that are involved in cell division, which localize at the division septum and assemble into highly regulated complexes. Current knowledge of these complexes has been greatly facilitated by super-resolution imaging using fluorescent protein fusions. Herein, we demonstrate with FtsZ that single-molecule PALM images can be obtained in-vivo using a genetically fused nanotag (...
Source: Current Genetics - April 6, 2023 Category: Genetics & Stem Cells Authors: Emma Westlund Axel Bergenstr åle Alaska Pokhrel Helena Chan Ulf Skoglund Daniel O Daley Bill S öderström Source Type: research
