The antifungal effect induced by itraconazole in Candida parapsilosis largely depends on the oxidative stress generated at the mitochondria
Curr Genet. 2023 Jun;69(2-3):165-173. doi: 10.1007/s00294-023-01269-z. Epub 2023 Apr 29.ABSTRACTIn Candida parapsilosis, homozygous disruption of the two genes encoding trehalase activity increased the susceptibility to Itraconazole compared with the isogenic parental strain. The fungicidal effect of this azole can largely be counteracted by preincubating growing cells with rotenone and the protonophore 2,4-Dinitrophenol. In turn, measurement of endogenous reactive oxygen species formation by flow cytometry confirmed that Itraconazole clearly induced an internal oxidative stress, which can be significantly abolished in rot...
Source: Current Genetics - April 29, 2023 Category: Genetics & Stem Cells Authors: M ª Luz Muñoz-Megías Ruth S ánchez-Fresneda Francisco Solano Sergi Maicas Mar ía Martínez-Esparza Juan-Carlos Arg üelles Source Type: research
The antifungal effect induced by itraconazole in Candida parapsilosis largely depends on the oxidative stress generated at the mitochondria
Curr Genet. 2023 Jun;69(2-3):165-173. doi: 10.1007/s00294-023-01269-z. Epub 2023 Apr 29.ABSTRACTIn Candida parapsilosis, homozygous disruption of the two genes encoding trehalase activity increased the susceptibility to Itraconazole compared with the isogenic parental strain. The fungicidal effect of this azole can largely be counteracted by preincubating growing cells with rotenone and the protonophore 2,4-Dinitrophenol. In turn, measurement of endogenous reactive oxygen species formation by flow cytometry confirmed that Itraconazole clearly induced an internal oxidative stress, which can be significantly abolished in rot...
Source: Current Genetics - April 29, 2023 Category: Genetics & Stem Cells Authors: M ª Luz Muñoz-Megías Ruth S ánchez-Fresneda Francisco Solano Sergi Maicas Mar ía Martínez-Esparza Juan-Carlos Arg üelles Source Type: research
The antifungal effect induced by itraconazole in Candida parapsilosis largely depends on the oxidative stress generated at the mitochondria
Curr Genet. 2023 Jun;69(2-3):165-173. doi: 10.1007/s00294-023-01269-z. Epub 2023 Apr 29.ABSTRACTIn Candida parapsilosis, homozygous disruption of the two genes encoding trehalase activity increased the susceptibility to Itraconazole compared with the isogenic parental strain. The fungicidal effect of this azole can largely be counteracted by preincubating growing cells with rotenone and the protonophore 2,4-Dinitrophenol. In turn, measurement of endogenous reactive oxygen species formation by flow cytometry confirmed that Itraconazole clearly induced an internal oxidative stress, which can be significantly abolished in rot...
Source: Current Genetics - April 29, 2023 Category: Genetics & Stem Cells Authors: M ª Luz Muñoz-Megías Ruth S ánchez-Fresneda Francisco Solano Sergi Maicas Mar ía Martínez-Esparza Juan-Carlos Arg üelles Source Type: research
The antifungal effect induced by itraconazole in Candida parapsilosis largely depends on the oxidative stress generated at the mitochondria
Curr Genet. 2023 Jun;69(2-3):165-173. doi: 10.1007/s00294-023-01269-z. Epub 2023 Apr 29.ABSTRACTIn Candida parapsilosis, homozygous disruption of the two genes encoding trehalase activity increased the susceptibility to Itraconazole compared with the isogenic parental strain. The fungicidal effect of this azole can largely be counteracted by preincubating growing cells with rotenone and the protonophore 2,4-Dinitrophenol. In turn, measurement of endogenous reactive oxygen species formation by flow cytometry confirmed that Itraconazole clearly induced an internal oxidative stress, which can be significantly abolished in rot...
Source: Current Genetics - April 29, 2023 Category: Genetics & Stem Cells Authors: M ª Luz Muñoz-Megías Ruth S ánchez-Fresneda Francisco Solano Sergi Maicas Mar ía Martínez-Esparza Juan-Carlos Arg üelles Source Type: research
The antifungal effect induced by itraconazole in Candida parapsilosis largely depends on the oxidative stress generated at the mitochondria
Curr Genet. 2023 Apr 29. doi: 10.1007/s00294-023-01269-z. Online ahead of print.ABSTRACTIn Candida parapsilosis, homozygous disruption of the two genes encoding trehalase activity increased the susceptibility to Itraconazole compared with the isogenic parental strain. The fungicidal effect of this azole can largely be counteracted by preincubating growing cells with rotenone and the protonophore 2,4-Dinitrophenol. In turn, measurement of endogenous reactive oxygen species formation by flow cytometry confirmed that Itraconazole clearly induced an internal oxidative stress, which can be significantly abolished in rotenone-ex...
Source: Current Genetics - April 29, 2023 Category: Genetics & Stem Cells Authors: M ª Luz Muñoz-Megías Ruth S ánchez-Fresneda Francisco Solano Sergi Maicas Mar ía Martínez-Esparza Juan-Carlos Arg üelles Source Type: research
The antifungal effect induced by itraconazole in Candida parapsilosis largely depends on the oxidative stress generated at the mitochondria
Curr Genet. 2023 Apr 29. doi: 10.1007/s00294-023-01269-z. Online ahead of print.ABSTRACTIn Candida parapsilosis, homozygous disruption of the two genes encoding trehalase activity increased the susceptibility to Itraconazole compared with the isogenic parental strain. The fungicidal effect of this azole can largely be counteracted by preincubating growing cells with rotenone and the protonophore 2,4-Dinitrophenol. In turn, measurement of endogenous reactive oxygen species formation by flow cytometry confirmed that Itraconazole clearly induced an internal oxidative stress, which can be significantly abolished in rotenone-ex...
Source: Current Genetics - April 29, 2023 Category: Genetics & Stem Cells Authors: M ª Luz Muñoz-Megías Ruth S ánchez-Fresneda Francisco Solano Sergi Maicas Mar ía Martínez-Esparza Juan-Carlos Arg üelles Source Type: research
The antifungal effect induced by itraconazole in Candida parapsilosis largely depends on the oxidative stress generated at the mitochondria
Curr Genet. 2023 Apr 29. doi: 10.1007/s00294-023-01269-z. Online ahead of print.ABSTRACTIn Candida parapsilosis, homozygous disruption of the two genes encoding trehalase activity increased the susceptibility to Itraconazole compared with the isogenic parental strain. The fungicidal effect of this azole can largely be counteracted by preincubating growing cells with rotenone and the protonophore 2,4-Dinitrophenol. In turn, measurement of endogenous reactive oxygen species formation by flow cytometry confirmed that Itraconazole clearly induced an internal oxidative stress, which can be significantly abolished in rotenone-ex...
Source: Current Genetics - April 29, 2023 Category: Genetics & Stem Cells Authors: M ª Luz Muñoz-Megías Ruth S ánchez-Fresneda Francisco Solano Sergi Maicas Mar ía Martínez-Esparza Juan-Carlos Arg üelles Source Type: research
The antifungal effect induced by itraconazole in Candida parapsilosis largely depends on the oxidative stress generated at the mitochondria
Curr Genet. 2023 Apr 29. doi: 10.1007/s00294-023-01269-z. Online ahead of print.ABSTRACTIn Candida parapsilosis, homozygous disruption of the two genes encoding trehalase activity increased the susceptibility to Itraconazole compared with the isogenic parental strain. The fungicidal effect of this azole can largely be counteracted by preincubating growing cells with rotenone and the protonophore 2,4-Dinitrophenol. In turn, measurement of endogenous reactive oxygen species formation by flow cytometry confirmed that Itraconazole clearly induced an internal oxidative stress, which can be significantly abolished in rotenone-ex...
Source: Current Genetics - April 29, 2023 Category: Genetics & Stem Cells Authors: M ª Luz Muñoz-Megías Ruth S ánchez-Fresneda Francisco Solano Sergi Maicas Mar ía Martínez-Esparza Juan-Carlos Arg üelles Source Type: research
The N-terminal disordered region of ChsB regulates its efficient transport to the hyphal apical surface in Aspergillus nidulans
In this study, we showed that ChsB is phosphorylated in vivo. We characterized strains that produce ChsB using stepwise truncations of its N-terminal disordered region or deletions of some residues in that region and demonstrated its involvement in ChsB abundance on the hyphal apical surface and in hyphal tip localization. Furthermore, we showed that some deletions in this region affected the phosphorylation states of ChsB, raising the possibility that these states are important for the localization of ChsB to the hyphal surface and the growth of A. nidulans. Our findings indicate that ChsB transport is regulated by its N-...
Source: Current Genetics - April 18, 2023 Category: Genetics & Stem Cells Authors: Jingyun Jin Ryo Iwama Hiroyuki Horiuchi Source Type: research
The N-terminal disordered region of ChsB regulates its efficient transport to the hyphal apical surface in Aspergillus nidulans
In this study, we showed that ChsB is phosphorylated in vivo. We characterized strains that produce ChsB using stepwise truncations of its N-terminal disordered region or deletions of some residues in that region and demonstrated its involvement in ChsB abundance on the hyphal apical surface and in hyphal tip localization. Furthermore, we showed that some deletions in this region affected the phosphorylation states of ChsB, raising the possibility that these states are important for the localization of ChsB to the hyphal surface and the growth of A. nidulans. Our findings indicate that ChsB transport is regulated by its N-...
Source: Current Genetics - April 18, 2023 Category: Genetics & Stem Cells Authors: Jingyun Jin Ryo Iwama Hiroyuki Horiuchi Source Type: research
The N-terminal disordered region of ChsB regulates its efficient transport to the hyphal apical surface in Aspergillus nidulans
In this study, we showed that ChsB is phosphorylated in vivo. We characterized strains that produce ChsB using stepwise truncations of its N-terminal disordered region or deletions of some residues in that region and demonstrated its involvement in ChsB abundance on the hyphal apical surface and in hyphal tip localization. Furthermore, we showed that some deletions in this region affected the phosphorylation states of ChsB, raising the possibility that these states are important for the localization of ChsB to the hyphal surface and the growth of A. nidulans. Our findings indicate that ChsB transport is regulated by its N-...
Source: Current Genetics - April 18, 2023 Category: Genetics & Stem Cells Authors: Jingyun Jin Ryo Iwama Hiroyuki Horiuchi Source Type: research
The N-terminal disordered region of ChsB regulates its efficient transport to the hyphal apical surface in Aspergillus nidulans
In this study, we showed that ChsB is phosphorylated in vivo. We characterized strains that produce ChsB using stepwise truncations of its N-terminal disordered region or deletions of some residues in that region and demonstrated its involvement in ChsB abundance on the hyphal apical surface and in hyphal tip localization. Furthermore, we showed that some deletions in this region affected the phosphorylation states of ChsB, raising the possibility that these states are important for the localization of ChsB to the hyphal surface and the growth of A. nidulans. Our findings indicate that ChsB transport is regulated by its N-...
Source: Current Genetics - April 18, 2023 Category: Genetics & Stem Cells Authors: Jingyun Jin Ryo Iwama Hiroyuki Horiuchi Source Type: research
The N-terminal disordered region of ChsB regulates its efficient transport to the hyphal apical surface in Aspergillus nidulans
In this study, we showed that ChsB is phosphorylated in vivo. We characterized strains that produce ChsB using stepwise truncations of its N-terminal disordered region or deletions of some residues in that region and demonstrated its involvement in ChsB abundance on the hyphal apical surface and in hyphal tip localization. Furthermore, we showed that some deletions in this region affected the phosphorylation states of ChsB, raising the possibility that these states are important for the localization of ChsB to the hyphal surface and the growth of A. nidulans. Our findings indicate that ChsB transport is regulated by its N-...
Source: Current Genetics - April 18, 2023 Category: Genetics & Stem Cells Authors: Jingyun Jin Ryo Iwama Hiroyuki Horiuchi Source Type: research
The N-terminal disordered region of ChsB regulates its efficient transport to the hyphal apical surface in Aspergillus nidulans
In this study, we showed that ChsB is phosphorylated in vivo. We characterized strains that produce ChsB using stepwise truncations of its N-terminal disordered region or deletions of some residues in that region and demonstrated its involvement in ChsB abundance on the hyphal apical surface and in hyphal tip localization. Furthermore, we showed that some deletions in this region affected the phosphorylation states of ChsB, raising the possibility that these states are important for the localization of ChsB to the hyphal surface and the growth of A. nidulans. Our findings indicate that ChsB transport is regulated by its N-...
Source: Current Genetics - April 18, 2023 Category: Genetics & Stem Cells Authors: Jingyun Jin Ryo Iwama Hiroyuki Horiuchi Source Type: research
The N-terminal disordered region of ChsB regulates its efficient transport to the hyphal apical surface in Aspergillus nidulans
In this study, we showed that ChsB is phosphorylated in vivo. We characterized strains that produce ChsB using stepwise truncations of its N-terminal disordered region or deletions of some residues in that region and demonstrated its involvement in ChsB abundance on the hyphal apical surface and in hyphal tip localization. Furthermore, we showed that some deletions in this region affected the phosphorylation states of ChsB, raising the possibility that these states are important for the localization of ChsB to the hyphal surface and the growth of A. nidulans. Our findings indicate that ChsB transport is regulated by its N-...
Source: Current Genetics - April 18, 2023 Category: Genetics & Stem Cells Authors: Jingyun Jin Ryo Iwama Hiroyuki Horiuchi Source Type: research
