In-plane and out-of-plane gigahertz sound velocities of saturated and unsaturated phospholipid bilayers from cryogenic to room temperatures
Chem Phys Lipids. 2023 Aug 12:105335. doi: 10.1016/j.chemphyslip.2023.105335. Online ahead of print.ABSTRACTHere, we examined the gigahertz sound velocities of hydrated multibilayers of saturated (1,2-dimyristoyl-sn-glycero-3-phosphocholine, DMPC) and unsaturated (1,2-dioleoyl-sn-glycero-3-phosphocholine, DOPC) phospholipids by Brillouin spectroscopy. Out-of-plane and in-plane (lateral) phonons were studied independently of each other. Similar strong temperature dependences of the sound velocities were found for phonons of both types. The sound velocities in the low-temperature limit were two-fold higher than that at physi...
Source: Chemistry and Physics of Lipids - August 14, 2023 Category: Lipidology Authors: E A Dobrynina V A Zykova N V Surovtsev Source Type: research

Characterization of conformational states of POPC and DPPC < sub > d62 < /sub > in POPC/DPPC < sub > d62 < /sub > /cholesterol mixtures using Raman spectroscopy
Chem Phys Lipids. 2023 Aug 12:105337. doi: 10.1016/j.chemphyslip.2023.105337. Online ahead of print.ABSTRACTConformational states of phospholipid chains in ternary mixtures of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), deuterated 1,2-dipalmitoyl-d62-sn-glycero-3-phosphocholine (DPPCd62), and cholesterol (Chol) were studied by Raman spectroscopy. Parameters of Raman peaks sensitive to conformational order have been used to determine chain order for mixtures over a wide range of compositions. A ternary diagram of fractions of phospholipid chains in conformationally ordered and disordered states has been constru...
Source: Chemistry and Physics of Lipids - August 14, 2023 Category: Lipidology Authors: Yu V Zaytseva I V Zaytseva N V Surovtsev Source Type: research

In-plane and out-of-plane gigahertz sound velocities of saturated and unsaturated phospholipid bilayers from cryogenic to room temperatures
Chem Phys Lipids. 2023 Aug 12:105335. doi: 10.1016/j.chemphyslip.2023.105335. Online ahead of print.ABSTRACTHere, we examined the gigahertz sound velocities of hydrated multibilayers of saturated (1,2-dimyristoyl-sn-glycero-3-phosphocholine, DMPC) and unsaturated (1,2-dioleoyl-sn-glycero-3-phosphocholine, DOPC) phospholipids by Brillouin spectroscopy. Out-of-plane and in-plane (lateral) phonons were studied independently of each other. Similar strong temperature dependences of the sound velocities were found for phonons of both types. The sound velocities in the low-temperature limit were two-fold higher than that at physi...
Source: Chemistry and Physics of Lipids - August 14, 2023 Category: Lipidology Authors: E A Dobrynina V A Zykova N V Surovtsev Source Type: research

Characterization of conformational states of POPC and DPPC < sub > d62 < /sub > in POPC/DPPC < sub > d62 < /sub > /cholesterol mixtures using Raman spectroscopy
Chem Phys Lipids. 2023 Aug 12:105337. doi: 10.1016/j.chemphyslip.2023.105337. Online ahead of print.ABSTRACTConformational states of phospholipid chains in ternary mixtures of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), deuterated 1,2-dipalmitoyl-d62-sn-glycero-3-phosphocholine (DPPCd62), and cholesterol (Chol) were studied by Raman spectroscopy. Parameters of Raman peaks sensitive to conformational order have been used to determine chain order for mixtures over a wide range of compositions. A ternary diagram of fractions of phospholipid chains in conformationally ordered and disordered states has been constru...
Source: Chemistry and Physics of Lipids - August 14, 2023 Category: Lipidology Authors: Yu V Zaytseva I V Zaytseva N V Surovtsev Source Type: research

In-plane and out-of-plane gigahertz sound velocities of saturated and unsaturated phospholipid bilayers from cryogenic to room temperatures
Chem Phys Lipids. 2023 Aug 12:105335. doi: 10.1016/j.chemphyslip.2023.105335. Online ahead of print.ABSTRACTHere, we examined the gigahertz sound velocities of hydrated multibilayers of saturated (1,2-dimyristoyl-sn-glycero-3-phosphocholine, DMPC) and unsaturated (1,2-dioleoyl-sn-glycero-3-phosphocholine, DOPC) phospholipids by Brillouin spectroscopy. Out-of-plane and in-plane (lateral) phonons were studied independently of each other. Similar strong temperature dependences of the sound velocities were found for phonons of both types. The sound velocities in the low-temperature limit were two-fold higher than that at physi...
Source: Chemistry and Physics of Lipids - August 14, 2023 Category: Lipidology Authors: E A Dobrynina V A Zykova N V Surovtsev Source Type: research

Solubilization of biomimetic lipid mixtures by some commonly used non-ionic detergents
Chem Phys Lipids. 2023 Sep;255:105327. doi: 10.1016/j.chemphyslip.2023.105327. Epub 2023 Jul 11.ABSTRACTDetergents are amphiphilic molecules often used to solubilize biological membranes and separate their components. Here we investigate the solubilization of lipid vesicles by the commonly used non-ionic detergents polyoxyethylene (20) oleyl ether (Brij 98), n-octyl-β-D-glucoside (OG), and n-dodecyl β-D maltoside (DDM) and compare the results with the standard detergent Triton X-100 (TX-100). The vesicles were composed of palmitoyl oleoyl phosphatidylcholine (POPC) or of a biomimetic ternary mixture of POPC, egg sphingom...
Source: Chemistry and Physics of Lipids - July 13, 2023 Category: Lipidology Authors: Amanda C Carit á Rafaela R M Cavalcanti Mariana S S Oliveira Karin A Riske Source Type: research

Solubilization of biomimetic lipid mixtures by some commonly used non-ionic detergents
Chem Phys Lipids. 2023 Jul 11;255:105327. doi: 10.1016/j.chemphyslip.2023.105327. Online ahead of print.ABSTRACTDetergents are amphiphilic molecules often used to solubilize biological membranes and separate their components. Here we investigate the solubilization of lipid vesicles by the commonly used non-ionic detergents polyoxyethylene (20) oleyl ether (Brij 98), n-octyl-β-D-glucoside (OG), and n-dodecyl β-D maltoside (DDM) and compare the results with the standard detergent Triton X-100 (TX-100). The vesicles were composed of palmitoyl oleoyl phosphatidylcholine (POPC) or of a biomimetic ternary mixture of POPC, egg ...
Source: Chemistry and Physics of Lipids - July 13, 2023 Category: Lipidology Authors: Amanda C Carit á Rafaela R M Cavalcanti Mariana S S Oliveira Karin A Riske Source Type: research

Solubilization of biomimetic lipid mixtures by some commonly used non-ionic detergents
Chem Phys Lipids. 2023 Jul 11;255:105327. doi: 10.1016/j.chemphyslip.2023.105327. Online ahead of print.ABSTRACTDetergents are amphiphilic molecules often used to solubilize biological membranes and separate their components. Here we investigate the solubilization of lipid vesicles by the commonly used non-ionic detergents polyoxyethylene (20) oleyl ether (Brij 98), n-octyl-β-D-glucoside (OG), and n-dodecyl β-D maltoside (DDM) and compare the results with the standard detergent Triton X-100 (TX-100). The vesicles were composed of palmitoyl oleoyl phosphatidylcholine (POPC) or of a biomimetic ternary mixture of POPC, egg ...
Source: Chemistry and Physics of Lipids - July 13, 2023 Category: Lipidology Authors: Amanda C Carit á Rafaela R M Cavalcanti Mariana S S Oliveira Karin A Riske Source Type: research

Solubilization of biomimetic lipid mixtures by some commonly used non-ionic detergents
Chem Phys Lipids. 2023 Jul 11;255:105327. doi: 10.1016/j.chemphyslip.2023.105327. Online ahead of print.ABSTRACTDetergents are amphiphilic molecules often used to solubilize biological membranes and separate their components. Here we investigate the solubilization of lipid vesicles by the commonly used non-ionic detergents polyoxyethylene (20) oleyl ether (Brij 98), n-octyl-β-D-glucoside (OG), and n-dodecyl β-D maltoside (DDM) and compare the results with the standard detergent Triton X-100 (TX-100). The vesicles were composed of palmitoyl oleoyl phosphatidylcholine (POPC) or of a biomimetic ternary mixture of POPC, egg ...
Source: Chemistry and Physics of Lipids - July 13, 2023 Category: Lipidology Authors: Amanda C Carit á Rafaela R M Cavalcanti Mariana S S Oliveira Karin A Riske Source Type: research

Solubilization of biomimetic lipid mixtures by some commonly used non-ionic detergents
Chem Phys Lipids. 2023 Jul 11;255:105327. doi: 10.1016/j.chemphyslip.2023.105327. Online ahead of print.ABSTRACTDetergents are amphiphilic molecules often used to solubilize biological membranes and separate their components. Here we investigate the solubilization of lipid vesicles by the commonly used non-ionic detergents polyoxyethylene (20) oleyl ether (Brij 98), n-octyl-β-D-glucoside (OG), and n-dodecyl β-D maltoside (DDM) and compare the results with the standard detergent Triton X-100 (TX-100). The vesicles were composed of palmitoyl oleoyl phosphatidylcholine (POPC) or of a biomimetic ternary mixture of POPC, egg ...
Source: Chemistry and Physics of Lipids - July 13, 2023 Category: Lipidology Authors: Amanda C Carit á Rafaela R M Cavalcanti Mariana S S Oliveira Karin A Riske Source Type: research

Solubilization of biomimetic lipid mixtures by some commonly used non-ionic detergents
Chem Phys Lipids. 2023 Jul 11;255:105327. doi: 10.1016/j.chemphyslip.2023.105327. Online ahead of print.ABSTRACTDetergents are amphiphilic molecules often used to solubilize biological membranes and separate their components. Here we investigate the solubilization of lipid vesicles by the commonly used non-ionic detergents polyoxyethylene (20) oleyl ether (Brij 98), n-octyl-β-D-glucoside (OG), and n-dodecyl β-D maltoside (DDM) and compare the results with the standard detergent Triton X-100 (TX-100). The vesicles were composed of palmitoyl oleoyl phosphatidylcholine (POPC) or of a biomimetic ternary mixture of POPC, egg ...
Source: Chemistry and Physics of Lipids - July 13, 2023 Category: Lipidology Authors: Amanda C Carit á Rafaela R M Cavalcanti Mariana S S Oliveira Karin A Riske Source Type: research

Solubilization of biomimetic lipid mixtures by some commonly used non-ionic detergents
Chem Phys Lipids. 2023 Jul 11;255:105327. doi: 10.1016/j.chemphyslip.2023.105327. Online ahead of print.ABSTRACTDetergents are amphiphilic molecules often used to solubilize biological membranes and separate their components. Here we investigate the solubilization of lipid vesicles by the commonly used non-ionic detergents polyoxyethylene (20) oleyl ether (Brij 98), n-octyl-β-D-glucoside (OG), and n-dodecyl β-D maltoside (DDM) and compare the results with the standard detergent Triton X-100 (TX-100). The vesicles were composed of palmitoyl oleoyl phosphatidylcholine (POPC) or of a biomimetic ternary mixture of POPC, egg ...
Source: Chemistry and Physics of Lipids - July 13, 2023 Category: Lipidology Authors: Amanda C Carit á Rafaela R M Cavalcanti Mariana S S Oliveira Karin A Riske Source Type: research

Solubilization of biomimetic lipid mixtures by some commonly used non-ionic detergents
Chem Phys Lipids. 2023 Jul 11;255:105327. doi: 10.1016/j.chemphyslip.2023.105327. Online ahead of print.ABSTRACTDetergents are amphiphilic molecules often used to solubilize biological membranes and separate their components. Here we investigate the solubilization of lipid vesicles by the commonly used non-ionic detergents polyoxyethylene (20) oleyl ether (Brij 98), n-octyl-β-D-glucoside (OG), and n-dodecyl β-D maltoside (DDM) and compare the results with the standard detergent Triton X-100 (TX-100). The vesicles were composed of palmitoyl oleoyl phosphatidylcholine (POPC) or of a biomimetic ternary mixture of POPC, egg ...
Source: Chemistry and Physics of Lipids - July 13, 2023 Category: Lipidology Authors: Amanda C Carit á Rafaela R M Cavalcanti Mariana S S Oliveira Karin A Riske Source Type: research

Solubilization of biomimetic lipid mixtures by some commonly used non-ionic detergents
Chem Phys Lipids. 2023 Jul 11;255:105327. doi: 10.1016/j.chemphyslip.2023.105327. Online ahead of print.ABSTRACTDetergents are amphiphilic molecules often used to solubilize biological membranes and separate their components. Here we investigate the solubilization of lipid vesicles by the commonly used non-ionic detergents polyoxyethylene (20) oleyl ether (Brij 98), n-octyl-β-D-glucoside (OG), and n-dodecyl β-D maltoside (DDM) and compare the results with the standard detergent Triton X-100 (TX-100). The vesicles were composed of palmitoyl oleoyl phosphatidylcholine (POPC) or of a biomimetic ternary mixture of POPC, egg ...
Source: Chemistry and Physics of Lipids - July 13, 2023 Category: Lipidology Authors: Amanda C Carit á Rafaela R M Cavalcanti Mariana S S Oliveira Karin A Riske Source Type: research

Solubilization of biomimetic lipid mixtures by some commonly used non-ionic detergents
Chem Phys Lipids. 2023 Jul 11;255:105327. doi: 10.1016/j.chemphyslip.2023.105327. Online ahead of print.ABSTRACTDetergents are amphiphilic molecules often used to solubilize biological membranes and separate their components. Here we investigate the solubilization of lipid vesicles by the commonly used non-ionic detergents polyoxyethylene (20) oleyl ether (Brij 98), n-octyl-β-D-glucoside (OG), and n-dodecyl β-D maltoside (DDM) and compare the results with the standard detergent Triton X-100 (TX-100). The vesicles were composed of palmitoyl oleoyl phosphatidylcholine (POPC) or of a biomimetic ternary mixture of POPC, egg ...
Source: Chemistry and Physics of Lipids - July 13, 2023 Category: Lipidology Authors: Amanda C Carit á Rafaela R M Cavalcanti Mariana S S Oliveira Karin A Riske Source Type: research