Monitoring galactolipid digestion and simultaneous changes in lipid-bile salt micellar organization by real-time NMR spectroscopy
Chem Phys Lipids. 2023 Nov 17:105361. doi: 10.1016/j.chemphyslip.2023.105361. Online ahead of print.ABSTRACTThe use of Nuclear Magnetic Resonance spectroscopy for studying lipid digestion in vitro most often consists of quantifying lipolysis products after they have been extracted from the reaction medium using organic solvents. However, the current sensitivity level of NMR spectrometers makes possible to avoid the extraction step and continuously quantify the lipids directly in the reaction medium. We used real-time 1H NMR spectroscopy and guinea pig pancreatic lipase-related protein 2 (GPLRP2) as biocatalyst to monitor i...
Source: Chemistry and Physics of Lipids - November 19, 2023 Category: Lipidology Authors: Moulay Sahaka Olivier Bornet Achille Marchand Dominique Lafont Brigitte Gontero Fr édéric Carrière H élène Launay Source Type: research
Monitoring galactolipid digestion and simultaneous changes in lipid-bile salt micellar organization by real-time NMR spectroscopy
Chem Phys Lipids. 2023 Nov 17:105361. doi: 10.1016/j.chemphyslip.2023.105361. Online ahead of print.ABSTRACTThe use of Nuclear Magnetic Resonance spectroscopy for studying lipid digestion in vitro most often consists of quantifying lipolysis products after they have been extracted from the reaction medium using organic solvents. However, the current sensitivity level of NMR spectrometers makes possible to avoid the extraction step and continuously quantify the lipids directly in the reaction medium. We used real-time 1H NMR spectroscopy and guinea pig pancreatic lipase-related protein 2 (GPLRP2) as biocatalyst to monitor i...
Source: Chemistry and Physics of Lipids - November 19, 2023 Category: Lipidology Authors: Moulay Sahaka Olivier Bornet Achille Marchand Dominique Lafont Brigitte Gontero Fr édéric Carrière H élène Launay Source Type: research
Elucidating the functional role of human ABHD16B lipase in regulating triacylglycerol mobilization and membrane lipid synthesis in Saccharomyces cerevisiae
In this study, we reported the overexpression of ABHD16B in Saccharomyces cerevisiae to determine its physiological relevance in lipid metabolism. Through in vivo [14C]acetate labeling experiments, we observed that overexpression of ABHD16B causes a decrease in cellular triacylglycerol (TAG) levels and a concurrent increase in phospholipid synthesis in wild-type cells. Mass spectrometry (LC-MS/MS) analysis further corroborated these findings, showing a significant decrease in TAGs with a carbon chain length of 48 and an increase in major phospholipid species, specifically 34:2, upon overexpression of ABHD16B. Confocal micr...
Source: Chemistry and Physics of Lipids - November 9, 2023 Category: Lipidology Authors: Raja Narayanasamy Dandamudi Usharani Ram Rajasekharan Source Type: research
Elucidating the functional role of human ABHD16B lipase in regulating triacylglycerol mobilization and membrane lipid synthesis in Saccharomyces cerevisiae
In this study, we reported the overexpression of ABHD16B in Saccharomyces cerevisiae to determine its physiological relevance in lipid metabolism. Through in vivo [14C]acetate labeling experiments, we observed that overexpression of ABHD16B causes a decrease in cellular triacylglycerol (TAG) levels and a concurrent increase in phospholipid synthesis in wild-type cells. Mass spectrometry (LC-MS/MS) analysis further corroborated these findings, showing a significant decrease in TAGs with a carbon chain length of 48 and an increase in major phospholipid species, specifically 34:2, upon overexpression of ABHD16B. Confocal micr...
Source: Chemistry and Physics of Lipids - November 9, 2023 Category: Lipidology Authors: Raja Narayanasamy Dandamudi Usharani Ram Rajasekharan Source Type: research
Elucidating the Functional Role of Human ABHD16B Lipase in Regulating Triacylglycerol Mobilization and Membrane Lipid Synthesis in Saccharomyces cerevisiae
In this study, we reported the overexpression of ABHD16B in Saccharomyces cerevisiae to determine its physiological relevance in lipid metabolism. Through in vivo [14C]acetate labeling experiments, we observed that overexpression of ABHD16B causes a decrease in cellular triacylglycerol (TAG) levels and a concurrent increase in phospholipid synthesis in wild-type cells. Mass spectrophotometry (LC-MS/MS) analysis further corroborated these findings, showing a significant decrease in TAGs with a carbon chain length of 48 and an increase in major phospholipid species, specifically 34:2, upon overexpression of ABHD16B. Confocal...
Source: Chemistry and Physics of Lipids - November 9, 2023 Category: Lipidology Authors: Raja Narayanasamy Dandamudi Usharani Ram Rajasekharan Source Type: research
Elucidating the Functional Role of Human ABHD16B Lipase in Regulating Triacylglycerol Mobilization and Membrane Lipid Synthesis in Saccharomyces cerevisiae
In this study, we reported the overexpression of ABHD16B in Saccharomyces cerevisiae to determine its physiological relevance in lipid metabolism. Through in vivo [14C]acetate labeling experiments, we observed that overexpression of ABHD16B causes a decrease in cellular triacylglycerol (TAG) levels and a concurrent increase in phospholipid synthesis in wild-type cells. Mass spectrophotometry (LC-MS/MS) analysis further corroborated these findings, showing a significant decrease in TAGs with a carbon chain length of 48 and an increase in major phospholipid species, specifically 34:2, upon overexpression of ABHD16B. Confocal...
Source: Chemistry and Physics of Lipids - November 9, 2023 Category: Lipidology Authors: Raja Narayanasamy Dandamudi Usharani Ram Rajasekharan Source Type: research
Elucidating the Functional Role of Human ABHD16B Lipase in Regulating Triacylglycerol Mobilization and Membrane Lipid Synthesis in Saccharomyces cerevisiae
In this study, we reported the overexpression of ABHD16B in Saccharomyces cerevisiae to determine its physiological relevance in lipid metabolism. Through in vivo [14C]acetate labeling experiments, we observed that overexpression of ABHD16B causes a decrease in cellular triacylglycerol (TAG) levels and a concurrent increase in phospholipid synthesis in wild-type cells. Mass spectrophotometry (LC-MS/MS) analysis further corroborated these findings, showing a significant decrease in TAGs with a carbon chain length of 48 and an increase in major phospholipid species, specifically 34:2, upon overexpression of ABHD16B. Confocal...
Source: Chemistry and Physics of Lipids - November 9, 2023 Category: Lipidology Authors: Raja Narayanasamy Dandamudi Usharani Ram Rajasekharan Source Type: research
Elucidating the Functional Role of Human ABHD16B Lipase in Regulating Triacylglycerol Mobilization and Membrane Lipid Synthesis in Saccharomyces cerevisiae
In this study, we reported the overexpression of ABHD16B in Saccharomyces cerevisiae to determine its physiological relevance in lipid metabolism. Through in vivo [14C]acetate labeling experiments, we observed that overexpression of ABHD16B causes a decrease in cellular triacylglycerol (TAG) levels and a concurrent increase in phospholipid synthesis in wild-type cells. Mass spectrophotometry (LC-MS/MS) analysis further corroborated these findings, showing a significant decrease in TAGs with a carbon chain length of 48 and an increase in major phospholipid species, specifically 34:2, upon overexpression of ABHD16B. Confocal...
Source: Chemistry and Physics of Lipids - November 9, 2023 Category: Lipidology Authors: Raja Narayanasamy Dandamudi Usharani Ram Rajasekharan Source Type: research
Elucidating the Functional Role of Human ABHD16B Lipase in Regulating Triacylglycerol Mobilization and Membrane Lipid Synthesis in Saccharomyces cerevisiae
In this study, we reported the overexpression of ABHD16B in Saccharomyces cerevisiae to determine its physiological relevance in lipid metabolism. Through in vivo [14C]acetate labeling experiments, we observed that overexpression of ABHD16B causes a decrease in cellular triacylglycerol (TAG) levels and a concurrent increase in phospholipid synthesis in wild-type cells. Mass spectrophotometry (LC-MS/MS) analysis further corroborated these findings, showing a significant decrease in TAGs with a carbon chain length of 48 and an increase in major phospholipid species, specifically 34:2, upon overexpression of ABHD16B. Confocal...
Source: Chemistry and Physics of Lipids - November 9, 2023 Category: Lipidology Authors: Raja Narayanasamy Dandamudi Usharani Ram Rajasekharan Source Type: research
Elucidating the Functional Role of Human ABHD16B Lipase in Regulating Triacylglycerol Mobilization and Membrane Lipid Synthesis in Saccharomyces cerevisiae
In this study, we reported the overexpression of ABHD16B in Saccharomyces cerevisiae to determine its physiological relevance in lipid metabolism. Through in vivo [14C]acetate labeling experiments, we observed that overexpression of ABHD16B causes a decrease in cellular triacylglycerol (TAG) levels and a concurrent increase in phospholipid synthesis in wild-type cells. Mass spectrophotometry (LC-MS/MS) analysis further corroborated these findings, showing a significant decrease in TAGs with a carbon chain length of 48 and an increase in major phospholipid species, specifically 34:2, upon overexpression of ABHD16B. Confocal...
Source: Chemistry and Physics of Lipids - November 9, 2023 Category: Lipidology Authors: Raja Narayanasamy Dandamudi Usharani Ram Rajasekharan Source Type: research
Saturation of fatty acids in phosphatidic acid uniquely alters transthyretin stability changing morphology and toxicity of amyloid fibrils
In this study, we investigated the effect of saturation of fatty acids (FAs) in PA on the rate of TTR aggregation. We also reveal the extent to which PAs with different length and saturation of FAs altered the morphology and secondary structure of TTR aggregates. Our results showed that TTR aggregation in the equimolar presence of PAs with different length and saturation of FAs yielded structurally and morphologically different fibrils compared to those formed in the lipid-free environment. We also found that PAs drastically lowered the toxicity of TTR aggregates formed in the presence of this phospholipid. These results s...
Source: Chemistry and Physics of Lipids - October 20, 2023 Category: Lipidology Authors: Abid Ali Kiryl Zhaliazka Tianyi Dou Aidan P Holman Dmitry Kurouski Source Type: research
The small-molecule kinase inhibitor ceritinib, unlike imatinib, causes a significant disturbance of lipid membrane integrity: A combined experimental and MD study
Chem Phys Lipids. 2023 Oct 18;257:105351. doi: 10.1016/j.chemphyslip.2023.105351. Online ahead of print.ABSTRACTCeritinib and imatinib are small-molecule protein kinase inhibitors which are applied as therapeutic agents against various diseases. The fundamentals of their clinical use, i.e. their pharmacokinetics as well as the mechanisms of the inhibition of the respective kinases, are relatively well studied. However, the interaction of the drugs with membranes, which can be a possible cause of side effects, has hardly been investigated so far. Therefore, we have characterized the interaction of both drugs with lipid memb...
Source: Chemistry and Physics of Lipids - October 20, 2023 Category: Lipidology Authors: Markus Fischer Meike Luck Max Werle Alexander Vogel Mohammad Bashawat Kai Ludwig Holger A Scheidt Peter M üller Source Type: research
Saturation of fatty acids in phosphatidic acid uniquely alters transthyretin stability changing morphology and toxicity of amyloid fibrils
In this study, we investigated the effect of saturation of fatty acids (FAs) in PA on the rate of TTR aggregation. We also reveal the extent to which PAs with different length and saturation of FAs altered the morphology and secondary structure of TTR aggregates. Our results showed that TTR aggregation in the equimolar presence of PAs with different length and saturation of FAs yielded structurally and morphologically different fibrils compared to those formed in the lipid-free environment. We also found that PAs drastically lowered the toxicity of TTR aggregates formed in the presence of this phospholipid. These results s...
Source: Chemistry and Physics of Lipids - October 20, 2023 Category: Lipidology Authors: Abid Ali Kiryl Zhaliazka Tianyi Dou Aidan P Holman Dmitry Kurouski Source Type: research
The small-molecule kinase inhibitor ceritinib, unlike imatinib, causes a significant disturbance of lipid membrane integrity: A combined experimental and MD study
Chem Phys Lipids. 2023 Oct 18;257:105351. doi: 10.1016/j.chemphyslip.2023.105351. Online ahead of print.ABSTRACTCeritinib and imatinib are small-molecule protein kinase inhibitors which are applied as therapeutic agents against various diseases. The fundamentals of their clinical use, i.e. their pharmacokinetics as well as the mechanisms of the inhibition of the respective kinases, are relatively well studied. However, the interaction of the drugs with membranes, which can be a possible cause of side effects, has hardly been investigated so far. Therefore, we have characterized the interaction of both drugs with lipid memb...
Source: Chemistry and Physics of Lipids - October 20, 2023 Category: Lipidology Authors: Markus Fischer Meike Luck Max Werle Alexander Vogel Mohammad Bashawat Kai Ludwig Holger A Scheidt Peter M üller Source Type: research
Saturation of fatty acids in phosphatidic acid uniquely alters transthyretin stability changing morphology and toxicity of amyloid fibrils
In this study, we investigated the effect of saturation of fatty acids (FAs) in PA on the rate of TTR aggregation. We also reveal the extent to which PAs with different length and saturation of FAs altered the morphology and secondary structure of TTR aggregates. Our results showed that TTR aggregation in the equimolar presence of PAs with different length and saturation of FAs yielded structurally and morphologically different fibrils compared to those formed in the lipid-free environment. We also found that PAs drastically lowered the toxicity of TTR aggregates formed in the presence of this phospholipid. These results s...
Source: Chemistry and Physics of Lipids - October 20, 2023 Category: Lipidology Authors: Abid Ali Kiryl Zhaliazka Tianyi Dou Aidan P Holman Dmitry Kurouski Source Type: research
