Periodontitis, coronary heart disease and myocardial infarction: treat one, benefit all
Periodontal disease is conventionally defined as an inflammatory condition affecting the tissues surrounding and supporting the teeth (i.e. gum and periodontium). Recent statistics show that the prevalence of this condition is continuously growing worldwide, thus raising severe healthcare concerns, not only for local problems emerging from poor oral health, but also for the potential risk of developing systemic complications. Therefore, this article aims to provide an update on the intriguing association between periodontitis, coronary heart disease (CHD) and/or myocardial infarction (MI). Taken together, the available pub...
Source: Blood Coagulation and Fibrinolysis - August 1, 2020 Category: Hematology Tags: REVIEW ARTICLES Source Type: research
d-Dimer serum levels as a biomarker associated for the lethality in patients with coronavirus disease 2019: a meta-analysis
No abstract available (Source: Blood Coagulation and Fibrinolysis)
Source: Blood Coagulation and Fibrinolysis - July 1, 2020 Category: Hematology Tags: LETTER TO THE EDITOR Source Type: research
Familial macro thrombocytopenia: role of genetics where morphology fails
We report a young girl with family history of thrombocytopenia and hearing loss who presented with kidney dysfunction and later developed acute lymphoblastic leukemia. She lacked the characteristic inclusion bodies in her blood granulocytes, however a diagnosis of MYH9-related Epstein syndrome was confirmed on genetic testing. In the background of known causal association of MYH9 gene in solid organ malignancies, the role of MYH9 gene variant in malignant transformation in the index case remains conjectural. (Source: Blood Coagulation and Fibrinolysis)
Source: Blood Coagulation and Fibrinolysis - July 1, 2020 Category: Hematology Tags: CASE REPORTS Source Type: research
Plasma viscosity pattern and erythrocyte aggregation in two patients with congenital afibrinogenemia
In this case report, we examine the behavior of plasma viscosity, explored at high and low shear rates, and erythrocyte aggregation in two patients with congenital afibrinogenemia, a clinical disorder firstly described in 1920 and that has an estimated incidence of 1 : 1–200 0000. The two hemorheological parameters examined by us showed a marked decrease in both patients, in one of whom erythrocyte aggregation was even undetectable. Keeping in mind that spontaneous thrombosis (venous and arterial) has been often described in congenital afibrinogenemia, it can be hypothesized that the decrease in plasma viscosity an...
Source: Blood Coagulation and Fibrinolysis - July 1, 2020 Category: Hematology Tags: CASE REPORTS Source Type: research
Analytical performance evaluation of Sysmex CS-2500 and Stago STA Compact
In this study, we aimed to evaluate performance characteristics of the CS-2500 in two coagulation tests [prothrombin time (PT) and activated partial thromboplastin time (aPTT)] at our hospital laboratory. PT and aPTT measurements were performed using the CS-2500 and STA-Compact Diagnostica Stago coagulometers (STA-Compact). Then, precision, accuracy, reference range verification, and method comparison statistics were performed. In the precision study, which was performed with normal and pathologic controls for the PT-international normalized ratio (INR) and aPTT tests, all coefficient of variation% were found less than 3.5...
Source: Blood Coagulation and Fibrinolysis - July 1, 2020 Category: Hematology Tags: ORIGINAL ARTICLES Source Type: research
Standard and derived rotational thromboelastometry parameters for prediction of disseminated intravascular coagulation in septic patients
Waiting for lab tests results for the calculation of disseminated intravascular coagulation (DIC) scores leads to unwanted delays in diagnosis. The use of rotational thromboelastometry (ROTEM) for this purpose would allow for a more rapid DIC diagnosis at the bedside. The aim of this study was to assess the ability of standard ROTEM parameters and calculated parameters from the ROTEM velocity curve to predict DIC. The retrospective observational study included 97 septic patients. Japanese Association for Acute Medicine score was used for DIC diagnosis and whole-blood ROTEM was performed at study inclusion. Univariate analy...
Source: Blood Coagulation and Fibrinolysis - July 1, 2020 Category: Hematology Tags: ORIGINAL ARTICLES Source Type: research
Rapamycin induces megakaryocytic differentiation through increasing autophagy in Dami cells
This study aims to explore the role of autophagy in megakaryocyte differentiation. To test our hypothesis, we used the Dami cell line in-vitro experiments. Rapamycin and Bafilomycin A1 were used to stimulate Dami cells. CD41 expression and apoptosis were analysed by flow cytometry. Autophagy-related proteins were detected by Western blotting. 12-O-Tetradecanoylphorbol 13-acetate-treated Dami cells can simulate endomitosis of megakaryocytes in vitro. Rapamycin-induced autophagic cell death was verified by LC3-II conversion upregulation. Meanwhile, Bafilomycin A1 blocked endomitosis and autophagy of Dami cells. Our results p...
Source: Blood Coagulation and Fibrinolysis - July 1, 2020 Category: Hematology Tags: ORIGINAL ARTICLES Source Type: research
Evaluation of analytic and clinical performance of thrombin–antithrombin complex and d-dimer assay in prognosis of acute ischemic stroke
To evaluate analytic and clinical performance of plasma thrombin–antithrombin complex (TAT) and d-dimer assay in assessing the severity and outcome of acute ischemic stroke. The prospective study was conducted and extended from January 2018 to December 2018. A total of 236 patients admitted within 24 h after neurologic symptoms onset were recruited. The median TAT and d-dimer levels were significantly higher in the acute ischemic stroke patients than in the controls. The average TAT levels in patients with mild, moderately severe and severe stroke were 1.75 [interquartile ranges (IQR), 1.1–2.6], 3.3 (IQR, 1.8–4.5) ...
Source: Blood Coagulation and Fibrinolysis - July 1, 2020 Category: Hematology Tags: ORIGINAL ARTICLES Source Type: research
Caesalpinia crista coat extract protects red blood cell from sodium nitrite-induced oxidative stress and exhibits antiplatelet activity
To understand the RBC protecting efficiency and antiplatelet activity of methanolic extract of Caesalpinia crista coat (MECCC). RBC-protecting activity of MECCC was evaluated using assays, such as DPPH, level of lipid peroxidation, protein carbonyl content, superoxide dismutase and catalase as a marker of oxidative stress whereas, platelet aggregation inhibition was performed using human platelet-rich plasma (PRP). MECCC showed about 76% of DPPH-scavenging activity, with an IC50 value of 71.89 μg/ml. The MECCC reduced the level of lipid peroxidation and protein carboxylation in RBC caused by NaNO2 in a dose-dependent ma...
Source: Blood Coagulation and Fibrinolysis - July 1, 2020 Category: Hematology Tags: ORIGINAL ARTICLES Source Type: research
Effectiveness of intravenous immunoglobulin use in heparin-induced thrombocytopenia
Heparin-induced thrombocytopenia (HIT) syndrome is an immune-mediated disorder producing thrombocytopenia and thrombosis, with or without prior exposure to heparin. Although avoidance of heparin products and nonheparin anticoagulants are used, immune-based therapies including intravenous immunoglobulin (IVIg) have been tried when the thrombocytopenia persists or there is breakthrough thrombosis. We sought to systematically review and analyze the published literature on use of IVIg in the treatment of HIT. A systematic search of PubMed, Google Scholar, EMBASE and SCOPUS for all study designs and reports were carried out fro...
Source: Blood Coagulation and Fibrinolysis - July 1, 2020 Category: Hematology Tags: REVIEW ARTICLE Source Type: research
Enzymatic deinduction phenomenon and clinical implications with a focus on direct-acting oral anticoagulants
The aim of the study was to describe the deinduction process and clinically relevant cases reported in the medical literature. Performed PubMed database search for key words ‘enzymatic deinduction’. Findings are summarized. Although many unwanted drug interactions occur from the additive effects of combined medications or through competition at binding sites, some interactions occur because of alterations in homeostasis of natural metabolizing pathways. Enzymatic induction is a natural process in which normal metabolizing enzymes are increased because of RNA signaling from xenobiotics. Strong inducing medications can l...
Source: Blood Coagulation and Fibrinolysis - May 11, 2020 Category: Hematology Tags: SHORT COMMUNICATION Source Type: research
An exceptional case of severe combined inherited thrombophilia successfully treated with rivaroxaban
We herein report the case of a young patient who presented with premature thromboembolic venous disease secondary to combined heterozygous G20210A prothrombin mutation, dual homozygosity for Factor V Leiden, and severe protein S deficiency. This association has never been reported to date and is likely to be exceptional, even in populations wherein these thrombophilia traits are more common. Long-term antithrombotic prophylaxis with rivaroxaban has proven successful in preventing clinical recurrence under prolonged treatment. (Source: Blood Coagulation and Fibrinolysis)
Source: Blood Coagulation and Fibrinolysis - May 11, 2020 Category: Hematology Tags: CASE REPORTS Source Type: research
Complete hemostasis achieved by factor XIII concentrate administration in a patient with bleeding after teeth extraction as a complication of aplastic anemia and chronic disseminated intravascular coagulation
Hemostatic treatment of disseminated intravascular coagulation (DIC) due to aortic aneurysm involves numerous difficulties. An 89-year-old man with aplastic anemia and chronic DIC developed periodontitis and loose teeth requiring extraction, after which hemostasis was difficult. Platelet concentrates and fresh-frozen plasma transfusions were ineffective, and there was a risk of hemorrhage; therefore, administration of anticoagulant agents for DIC was inappropriate. A decrease in factor XIII (FXIII) was discovered, and FXIII concentrate was administered, resulting in hemostasis together with wound healing. No complications ...
Source: Blood Coagulation and Fibrinolysis - May 11, 2020 Category: Hematology Tags: CASE REPORTS Source Type: research
Venous thromboembolism associated with hyperhomocysteinemia, homozygosity for the methylenetetrahydrofolate reductase 677C>T gene variant, and secondary polycythemia
The development of pulmonary embolism/deep vein thrombosis (DVT) in the extremities is influenced by various risk factors. Hyperhomocysteinemia is one such risk factor, which may be associated with vitamin B12/folate deficiency, or the methylenetetrahydrofolate reductase gene variant, 677C>T. Here, we report a 47-year-old male who developed pulmonary embolism/DVT, associated with hyperhomocysteinemia (plasma homocysteine: 71.9 nmol/ml; reference range: 6.3–18.9 nmol/ml) and was homozygous (T/T) for the methylenetetrahydrofolate reductase variant. Serum levels of vitamin B12 and folate were within the normal range, wh...
Source: Blood Coagulation and Fibrinolysis - May 11, 2020 Category: Hematology Tags: CASE REPORTS Source Type: research
Thrombosis risk of Alport syndrome patients: evaluation of cardiological, clinical, biochemical, genetic and possible causes of inherited thrombophilia and identification of a novel: COL4A3: variant
To evaluate cases with Alport syndrome for laboratory, radiological, ophthalmological, auditory tests, cardiological and inherited thrombophilia risk. Laboratory findings, abdominal and urinary ultrasonography, ophthalmological and auditory tests and cardiological examination of 21 Alport syndrome suspicious cases were performed. Also, collagen type IV alpha three chain (COL4A3) gene, four chain (COL4A4) gene and five chain (COL4A5) genes were sequenced by next-generation sequencing system. In addition, possible causes of inherited thrombophilia were evaluated. A novel (c.2806C>T/p.Gln936Ter) variation in COL4A3 gene was d...
Source: Blood Coagulation and Fibrinolysis - May 11, 2020 Category: Hematology Tags: ORIGINAL ARTICLES Source Type: research
