Lidocaine effects on coagulation assessed by whole blood rotational thromboelastometry
Lidocaine may be beneficial when added in solutions for the preservation of vascular grafts or solid organs as it has anti-inflammatory, endothelial protective, and antithrombotic effects. However, the mechanisms of lidocaine-induced changes in hemostasis were not elucidated until now. The aim of the study was to examine the effect of increasing concentrations of lidocaine on coagulation parameters and blood-clotting kinetics using velocity curves of clot formation assessed by rotational thromboelastometry. Ex-vivo blood coagulation using whole blood from healthy volunteers was studied with rotational thromboelastometry. F...
Source: Blood Coagulation and Fibrinolysis - February 11, 2021 Category: Hematology Tags: ORIGINAL ARTICLES Source Type: research
Comparison of different activators of coagulation by turbidity analysis of hereditary dysfibrinogenemia and controls
Turbidity analysis is widely used as a quantitative technique in hereditary dysfibrinogenemia. We aimed to compare several coagulation triggers in hereditary dysfibrinogenemia and control plasmas. We included 20 patients with hereditary dysfibrinogenemia, 19 with hotspot mutations Aα Arg35His (n = 9), Aα Arg35Cys (n = 2), γ Arg301His (n = 6), γ Arg301Cys (n = 2), and one with Aα Phe27Tyr, and a commercial pooled normal plasma. Fibrin polymerization was activated by bovine or human thrombin or tissue factor (TF), in the presence or absence of tissue type plasminogen activator. The lag time (min), slope ...
Source: Blood Coagulation and Fibrinolysis - February 11, 2021 Category: Hematology Tags: ORIGINAL ARTICLES Source Type: research
Combinations of fibrinolytic gene polymorphisms (plasminogen activator inhibitor type 1 4G/5G, factor XIII Val34Leu and angiotensin-converting enzyme I/D) in women with idiopathic infertility
During normal pregnancy depressed fibrinolytic system is caused by changes in many factors, which could be influenced by different gene polymorphisms. The aim of this study was to investigate the combination of fibrinolysis-related gene polymorphisms in women with idiopathic infertility. We genotype polymorphisms 4G/5G in plasminogen activator inhibitor type 1 (PAI-1), Val34Leu in factor XIII (FXIII) and I/D in angiotensin-converting enzyme (ACE) gene. The patients group consisted of 83 females with idiopathic infertility, while the control group included 121 females with at least one born child. The alleles and genotypes ...
Source: Blood Coagulation and Fibrinolysis - February 11, 2021 Category: Hematology Tags: ORIGINAL ARTICLES Source Type: research
Global sources of cryoprecipitate demonstrate variability in coagulant factor levels and functional hemostasis
In this study of global cryo sources, procoagulant activity and in-vitro clot formation varied by source. This could be explained by variance in production and storage protocols. Further study is warranted to assess functional variance in cryo to optimize and standardize the use of cryo products. (Source: Blood Coagulation and Fibrinolysis)
Source: Blood Coagulation and Fibrinolysis - February 11, 2021 Category: Hematology Tags: ORIGINAL ARTICLES Source Type: research
Derangement of the coagulation process using subclinical markers and viscoelastic measurements in critically ill patients with coronavirus disease 2019 pneumonia and non-coronavirus disease 2019 pneumonia
Systemic coagulation abnormalities including clotting activation and inhibition of anticoagulant factors have been observed in patients with pneumonia. In severe coronavirus disease 2019 (COVID-19) the alteration of coagulation parameters was associated with poor prognosis. We evaluated the difference in coagulopathy between critically ill patients with COVID-19 pneumonia (COVID group) and non-COVID-19 pneumonia (non-COVID group), using traditional coagulation markers and rotational thromboelastometry (ROTEM). Standard laboratory and ROTEM parameters were evaluated in 45 patients (20 COVID group patients and 25 non-COVID g...
Source: Blood Coagulation and Fibrinolysis - February 11, 2021 Category: Hematology Tags: ORIGINAL ARTICLES Source Type: research
Antiphospholipid antibodies in patients with coronavirus disease 2019 infection hospitalized in conventional unit
The objective of this work was to evaluate the incidence of aPL antibodies in patients hospitalized in conventional unit for coronavirus disease 2019 (COVID-19) infection and confirmed venous thromboembolic events (VTE) associated with aPL antibodies. 41 patients infected with COVID-19 were tested for aPL antibodies. None had reported history of aPL syndrome. Arterial and venous duplex ultrasound of lower limbs was performed in all patients at Day 0 and Day 5. All patients had antithrombotic-prophylaxis upon admission using lower molecular weight heparin with Enoxaparin. Biological parameters were collected and analyzed. N...
Source: Blood Coagulation and Fibrinolysis - February 11, 2021 Category: Hematology Tags: ORIGINAL ARTICLES Source Type: research
The impact of atorvastatin on dabigatran plasma levels in patients with atrial fibrillation
No abstract available (Source: Blood Coagulation and Fibrinolysis)
Source: Blood Coagulation and Fibrinolysis - January 1, 2021 Category: Hematology Tags: LETTERS TO THE EDITOR Source Type: research
Evaluating the potential benefits of the extravascular pool of factor IX
No abstract available (Source: Blood Coagulation and Fibrinolysis)
Source: Blood Coagulation and Fibrinolysis - January 1, 2021 Category: Hematology Tags: LETTERS TO THE EDITOR Source Type: research
Thromboelastometry-guided neuraxial anesthesia in a parturient with severe thrombocytopenia due to large granular lymphocytic leukemia
Severe thrombocytopenia (platelet count (Source: Blood Coagulation and Fibrinolysis)
Source: Blood Coagulation and Fibrinolysis - January 1, 2021 Category: Hematology Tags: CASE REPORT Source Type: research
Type II antithrombin deficiency caused by a novel missense mutation (p.Leu417Gln) in a Chinese family
The aim of the report was t
o explore the phenotype and genotype of a hereditary antithrombin deficient Chinese family. Functional and molecular analysis of the proband and his family members was performed. Online bioinformatics software was used to predict the pathogenicity of the novel mutation. ClustalX-2.1-win and PyMol software were applied to conservative analysis and generate molecular graphic images, respectively. Functional analysis had shown that the antithrombin (AT):A of the proband was reduced to 32% whereas AT:Ag was normal. Molecular analysis revealed a heterozygous missense mutation p. Leu417Gln in exon...
Source: Blood Coagulation and Fibrinolysis - January 1, 2021 Category: Hematology Tags: MUTATION REPORT Source Type: research
Microkinetic coagulation assays for human and zebrafish plasma
In conclusion, we established a microkinetic assay that could measure blood coagulation activity in models like zebrafish and human blood samples obtained from a finger prick in adults or heel prick in infants. (Source: Blood Coagulation and Fibrinolysis)
Source: Blood Coagulation and Fibrinolysis - January 1, 2021 Category: Hematology Tags: TECHNICAL REPORT Source Type: research
Coronavirus disease 2019 and coagulopathy: other prothrombotic coagulation factors
There is an increasing evidence supporting the existence of coagulopathy in coronavirus disease 2019 (COVID-19) patients. Most of reports are mainly focused on d-dimer. Our objective is to describe coagulation parameters in these patients that could be involved in a hypercoagulate state and to test platelet function to see if there are short closure times. We analyzed coagulation samples from 80 patients admitted with COVID-19 in our hospital. We also tested platelet function by closure times in a small subgroup of patients. Most of samples had increased d-dimer (96.2%) (median of d-dimer: 1158 ng/ml FEU), increased fi...
Source: Blood Coagulation and Fibrinolysis - January 1, 2021 Category: Hematology Tags: ORIGINAL ARTICLES Source Type: research
The incidence of venous thromboembolic events in trauma patients after tranexamic acid administration: an EAST multicenter study
To determine if there is a significant association between administration of tranexamic acid (TXA) in severely bleeding, injured patients, and venous thromboembolism (VTE), myocardial infarction (MI), or cerebrovascular accident (CVA). A multicenter, retrospective study was performed. Inclusion criteria were: age 18–80 years old and need for 5 units or more of blood in the first 24 h after injury. Exclusion criteria included: death within 24 h, pregnancy, administration of TXA more than 3 h following injury, and routine ultrasound surveillance for deep venous thrombosis. Incidence of VTE was the primary outcome. ...
Source: Blood Coagulation and Fibrinolysis - January 1, 2021 Category: Hematology Tags: ORIGINAL ARTICLES Source Type: research
Fibrin clot susceptibility to lysis is impaired after on-pump coronary artery by-pass grafting with tranexamic acid: clinical implications
Coronary artery bypass grafting (CABG) done on-pump may cause a significant blood loss. Low fibrinogen is associated with perioperative bleeding. The influence of cardiopulmonary bypass on fibrin clot properties is poorly investigated. We studied 55 patients with isolated coronary artery disease on aspirin undergoing on-pump CABG with tranexamic acid. Fibrinogen levels, fibrinolytic capacity expressed as clot lysis time (CLT), thrombin generation potential and platelet count were assessed before and after the surgery (prior to admission to the intensive care unit). A postoperative drop in haemoglobin (−30% from baseline)...
Source: Blood Coagulation and Fibrinolysis - January 1, 2021 Category: Hematology Tags: ORIGINAL ARTICLES Source Type: research
Factors associated with deep venous thrombosis in patients infected with coronavirus disease 2019: a meta-analysis
Coronavirus disease 2019 (COVID-19) has affected more than 6 million patients worldwide. Deep venous thrombosis (DVT) has been increasingly recognized complication in these patients and is associated with increased morbidity and mortality. However, the factors associated with development of DVT in patients with COVID-19 have not been elucidated due to the novelty of the virus. We performed a meta-analysis of published studies comparing laboratory results in COVID-19 patients with and without DVT with the aim of identifying risk factors. We searched major databases for studies evaluating DVT in COVID-positive patients and p...
Source: Blood Coagulation and Fibrinolysis - January 1, 2021 Category: Hematology Tags: ORIGINAL ARTICLES Source Type: research
