Effects of Momordica charantia exosomes on platelet activation, adhesion, and aggregation
In this study, we have attempted to study the effects of extracted Momordica charantia exosomes (MCEs) on platelet activation, adhesion, and aggregation. Adult platelets isolated from healthy individuals were dose-dependently treated with MCEs (0.1, 40, and 200 μg/ml). We performed flow cytometry to detect the expression of platelet activation protein marker-activated GP IIb/IIIa (PAC-1) and P-selectin (CD62P). Platelet adhesion was analyzed through fluorescence labeling assays. The effect of MCEs on platelet-mediated cell migration of HCT116 cells was observed by transwell. Furthermore, the MCAO model of Sprague–Dawl...
Source: Blood Coagulation and Fibrinolysis - October 1, 2022 Category: Hematology Tags: ORIGINAL ARTICLES Source Type: research
Labeling fibrin fibers with beads alter single fibrin fiber lysis, external clot lysis, and produce large fibrin aggregates upon lysis
Fluorescent beads are often used as a tool for visualizing fibrin fibers in single fiber mechanics studies and studies of single fiber lysis. Here we investigate the effect of beads on fibrin fiber lysis and extensibility to enhance understanding of this common research technique. We selected beads of the same diameter as those used in previous studies, as well as, beads of similar size to microparticles in the bloodstream. We used fluorescence microscopy, atomic force microscopy (AFM), and scanning electron microscopy (SEM) to quantify changes in fiber lysis, fiber extensibility, and clot structure in the presence and abs...
Source: Blood Coagulation and Fibrinolysis - October 1, 2022 Category: Hematology Tags: ORIGINAL ARTICLES Source Type: research
CREST: clinical bleeding and risk evaluation in hematology–oncology patients: a systematic review and meta-analysis of thromboelastography's role
Thrombocytopenia and bleeding are common complications of hematologic malignancies. Often, prophylactic platelets are administered to minimize bleeding risk, based on total platelet count (TPC). However, TPC is a poor predictor, and does not provide rapid information. This review presents a novel prospective in the use of point-of-care viscoelastic studies to assess bleeding risk and guide transfusion therapy in a haematological oncological population, where its use can be extended to a ward level as a bedside test. Monitoring TEG maximum amplitude trends may be useful to guide transfusion protocols, especially for patient...
Source: Blood Coagulation and Fibrinolysis - October 1, 2022 Category: Hematology Tags: REVIEW ARTICLE Source Type: research
The coexistence of antiβ2 glycoprotein 1 antibody antibody has no effect on hemophilia A patient
No abstract available (Source: Blood Coagulation and Fibrinolysis)
Source: Blood Coagulation and Fibrinolysis - August 21, 2022 Category: Hematology Tags: LETTER TO THE EDITOR Source Type: research
Challenging treatment for refractory acquired haemophilia A complicated with severe severe acute respiratory coronavirus 2 infection
Immunosuppressive treatment and bypassing agents are used to treat acquired haemophilia A (AHA). On the other hand, COVID-19 infection induces a hypercoagulable state. Managing bleeding, risk of thrombosis, bypassing agents, active infection and immunosuppressive treatment can be challenging. A 72-year-old man was diagnosed with acquired hemophilia A. He received steroids, rituximab and recombinant activated factor VII (rFVIIa). He developed severe SARS-CoV-2 infection. Due to thrombotic risk, he received low-molecular-weight heparin (LMWH) and developed an iliopsoas hematoma. Because of the risk of thrombosis, treatment w...
Source: Blood Coagulation and Fibrinolysis - August 21, 2022 Category: Hematology Tags: CASE REPORT Source Type: research
A novel F13A1 gene mutation (Arg208Pro) in a Chinese patient with factor XIII deficiency
We reported a novel mutation in the F13A1 gene, which can flesh out the mutant library. (Source: Blood Coagulation and Fibrinolysis)
Source: Blood Coagulation and Fibrinolysis - August 21, 2022 Category: Hematology Tags: MUTATION REPORT Source Type: research
Characterization of thrombophilia-related plasmas evaluated by anticoagulants-mediated thrombin and plasmin generation assays
Disturbances in the balance between coagulation, anticoagulation and fibrinolysis may lead to thrombosis or haemorrhage. Simultaneous assessments of thrombin and plasmin facilitate overall understandings of pathological haemostasis, especially for thrombophilia. Here, we characterized coagulation–fibrinolysis potentials in plasmas with thrombophilia using anticoagulants-mediated thrombin-plasmin generation assay (T/P-GA). T/P-GA was initiated by adding tissue factor, tissue-type plasminogen activator and anticoagulants [recombinant-thrombomodulin (rTM), activated protein (P)C (APC) and antithrombin (AT)], followed by sim...
Source: Blood Coagulation and Fibrinolysis - August 21, 2022 Category: Hematology Tags: ORIGINAL ARTICLES Source Type: research
Left ventricular-aortic angle is associated with platelet reactivity in patients with aortic stenosis
The impact of aortic stenosis on platelet reactivity is unclear. Previous studies reported contradicting results. The reason for this is unknown. It is known that flow alterations enhance platelet reactivity. A steep left ventricular-aortic angle (LV-AO-angle) is associated with turbulent flow in the aorta ascendens. Therefore, in this study, we hypothesized that LV-AO-angle is associated with platelet reactivity in patients with severe aortic stenosis. We included 289 patients with severe aortic stenosis and performed cardiac computertomography to assess the LV-AO-angle. Platelet function was evaluated by light transmissi...
Source: Blood Coagulation and Fibrinolysis - August 21, 2022 Category: Hematology Tags: ORIGINAL ARTICLES Source Type: research
Evaluation of paediatric immune thrombocytopenia patients with clinical and laboratory findings: emphasizing the role of monocytosis
We aimed to investigate the relationship between demographics, clinical features, laboratory findings including monocytosis and clinical course in children with immune thrombocytopenia (ITP). Data of 100 ITP patients were analysed. Complete blood count findings of the patients at certain time points were evaluated to classify the disease as acute, persistent and chronic. An effect of sex on chronicity was not observed (P = 0.166). Of the patients enrolled in the study, 38% (n = 38) had chronic course. The mean age of patients with the chronic course was 7 ± 4.1 years, which was significantly higher than the ...
Source: Blood Coagulation and Fibrinolysis - August 21, 2022 Category: Hematology Tags: ORIGINAL ARTICLES Source Type: research
Molecular heterogeneity of factor XI deficiency in Tunisia
This report represents the first genetic study analyzing the molecular characteristics of factor XI deficiency within Tunisian population. Identification of the Jewish type II mutation in two families, as well as one missense previously reported mutation and one novel mutation confirmed the genetic heterogeneity of this disorder. Screening a large number of Tunisian factor XI deficient would reveal the spectrum mutations causing factor XI deficiency in Tunisia. (Source: Blood Coagulation and Fibrinolysis)
Source: Blood Coagulation and Fibrinolysis - August 21, 2022 Category: Hematology Tags: ORIGINAL ARTICLES Source Type: research
Risk factors for recurrent venous thromboembolism: a real-world analysis
This study was conducted in patients treated for initial venous thromboembolism (VTE) for approximately 6 months to enhance understanding of the risk factors to inform clinical decision-making about long-term anticoagulation therapy. This retrospective cohort study was conducted using a large administrative claims database in the United States. A Cox proportional hazards model was used to examine demographic and clinical characteristics associated with recurrent VTE. A total of 13 831 patients had an index VTE event, and recurrent VTE occurred in 844 (6.1%) of these patients over a median follow-up of 22.8 months. Ba...
Source: Blood Coagulation and Fibrinolysis - August 21, 2022 Category: Hematology Tags: ORIGINAL ARTICLES Source Type: research
Pulmonary vein stump thrombosis after lung resection for lung cancer: clinical features and outcome
Pulmonary vein stump thrombosis (PVST) is uncommonly encountered postoperative in-situ thrombosis in the stump of pulmonary veins after lung resection. Data regarding the incidence and clinical behaviour of PVST are scarce. Thus, this study aims to investigate the incidence, clinical characteristics and outcome of PVST after lung resection in patients with lung cancer. Follow-up enhanced chest computed tomography (CT) scans acquired after the surgery were retrospectively reviewed to determine PVST presence for patients with lung cancer who underwent lung resection in two tertiary referral centres. Out of the 1885 patients ...
Source: Blood Coagulation and Fibrinolysis - August 21, 2022 Category: Hematology Tags: ORIGINAL ARTICLES Source Type: research
Primary immune thrombocytopenia: a ‘diagnosis of exclusion’?
Current diagnosis of primary immune thrombocytopenia (ITP) is presumptive, centered on excluding other causes of thrombocytopenia. The diagnosis of ITP is challenging because of the wide range of potential inherited and acquired causes of thrombocytopenia. The treatment of ITP is empiric with steroids, high-dose immunoglobulin, immunosuppressants and thrombopoietin agonists with potential side effects. We searched Medline and Cochrane databases, reviewed the study data and analyzed the individual diagnostic tests for their evidence-based role in the diagnosis of ITP. We then analyzed the strength of the scientific evidence...
Source: Blood Coagulation and Fibrinolysis - August 21, 2022 Category: Hematology Tags: REVIEW ARTICLE Source Type: research
Postacute elevation of D-dimer levels in severe acute respiratory syndrome coronavirus 2-positive nonhospitalized patients with mild symptoms
No abstract available (Source: Blood Coagulation and Fibrinolysis)
Source: Blood Coagulation and Fibrinolysis - July 1, 2022 Category: Hematology Tags: RESEARCH LETTER Source Type: research
Clinical phenotype and F7 gene genotype in 40 Tunisian patients with congenital factor VII deficiency
This study aimed to determine the genetic alterations of 40 Tunisian patients and to evaluate their relationships with the collected clinical and biological data. Forty FVII-deficient Tunisian patients have been included in this study. First, diagnosis of the FVII deficiency was made on the basis of FVII coagulant activity (FVII:c) levels performed using the prothrombin time assay. Then, clinical and anamnesis data were set up and filed out from the regional registry of bleeding disorders and the medical file of each patient. Finally, genetic alterations were determined by direct sequencing of the coding regions, intron/ex...
Source: Blood Coagulation and Fibrinolysis - July 1, 2022 Category: Hematology Tags: ORIGINAL ARTICLES Source Type: research
